BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Hongsen Zhang,Gang Liu,Jian Zhang,Jie Bao
High titer gluconic acid and xylonic acid were simultaneously fermented by Gluconobacter oxydans DSM 2003 using corn stover feedstock after dry dilute sulfuric acid pretreatment, biodetoxification and high solids content hydrolysis. Maximum sodium gluconate and xylonate were produced at the titer of 132.46 g/L and 38.86 g/L with the overall yield of 97.12% from glucose and 90.02% from xylose, respectively. The drawbacks of filamentous fungus Aspergillus niger including weak inhibitor tolerance, large pellet formation and no xylose utilization were solved by using the bacterium strain G. oxydans . The obtained sodium gluconate/xylonate product was highly competitive as cement retarder additive to the commercial product from corn feedstock. The techno-economic analysis (TEA) based on the Aspen Plus modeling was performed and the minimum sodium gluconate/xylonate product selling price (MGSP) was calculated as $0.404/kg. This study provided a practical and economic competitive process of lignocellulose utilization for production of value-added biobased chemicals.
研究领域:
生物工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hongsen Zhang,Jian Zhang,Jie Bao
This study reported a high titer gluconic acid fermentation using dry dilute acid pretreated corn stover (DDAP) hydrolysate without detoxification. The selected fermenting strain Aspergillus niger SIIM M276 was capable of inhibitor degradation thus no detoxification on pretreated corn stover was required. Parameters of gluconic acid fermentation in corn stover hydrolysate were optimized in flasks and in fermentors to achieve 76.67 g/L gluconic acid with overall yield of 94.91%. The sodium gluconate obtained from corn stover was used as additive for extending setting time of cement mortar and similar function was obtained with starch based sodium gluconate. This study provided the first high titer gluconic acid production from lignocellulosic feedstock with potential of industrial applications.
研究领域:
生物技术
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2020
·Vol. 22
·期 8
·2588-2597
·
IF 9.48
Xintong Meng,Zengyong Li,Di Li,Yiming Huang,Jiaojiao Ma,Chuanfu Liu,Xinwen Peng
Catalytic and selective oxidation of monosaccharides into value-added sugar acids is highly desirable from both scientific and industrial perspectives. Here, we report a hierarchical porous carbon (NC-3) supported gold catalyst (Au/NC-3) and its application in the base-free aerobic oxidation of D-glucose and D-xylose into value-added sugar acids under mild conditions (100 °C). The catalyst exhibited excellent catalytic performance with the yields of D-gluconic acid and D-xylonic acid reaching 97.62% and 98.76%, respectively. We studied the effect of the ball-milling process on the hierarchical porous preparation, which is critical to the formation of the fibrous structure during the pyrosis process. This specific structure provides active sites for Au adsorption. DFT calculation revealed that the Au NPs prefer to anchor on pyridinic N sites; meanwhile, the initial reactants and essential intermediates are found to be well stabilized by Au/NC-3. Moreover, upon recycling, gold was stable toward the recovery procedure. In this work, we provide a green method for an improved generally selective oxidation of D-glucose and D-xylose using Au/NC-3 as an efficient heterogeneous catalyst.
研究领域:
化学
·
催化科学
✓ 已验证
DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 7
·857
·
IF 4.9
Bolun Li,Mengyan Wang,Huixiang Luo,Kaina Li,Yanlong Jia,Mingjie Fu,Chenyu Jiang,Shuangquan Yao,Yongjun Yin
As a carbon-neutral and renewable raw material, cellulose can be transformed into biomass fuels to reduce the dependence on fossil fuels and carbon dioxide emissions. In view of harsh reaction conditions, low selectivity of product, and easy deactivation of the catalyst, this study studied the use of photothermal catalytic technology to convert cellulose into bio-oil rich in levulinic acid. It was discovered that a synergistic effect between heating and photocatalysis is present in cellulose degradation. Different metals were loaded on carbon nanotubes doped with titanium dioxide to prepare different photothermal catalysts, and their catalytic effects on cellulose were compared. It was found that TiO2-CNT loaded with platinum metal exhibited the highest catalytic performance. By adopting Pt/TiO2-CNT as the catalyst, the conversion rate of bio-oil reached 99.44%, and the selectivity of LA reached 44.41% at 220 °C for 3 h. As the photothermal catalysis increased the H/C ratio and decreased the O/C ratio of the liquid product, the calorific value reached 21.01 MJ/kg. This study can promote the further industrial application of lignocellulose to prepare fuel oil and decrease the environmental pollution caused by the massive consumption of fossil fuels.
研究领域:
能源科学
·
催化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2015
·
IF 3.476
Guizhuan Xu,Chun Chang,Shuqi Fang,Xiaojian Ma
Cellulose reactivity in ethanol at elevated temperature (170–210 °C) was investigated in this study. Water and acid catalyst can improve the solubilization and the conversion of cellulose in ethanol. In ethanol/water medium, more humic solids will be formed, but the amounts of diethyl ether decreased greatly. In ethanol medium, the ethanolysis reaction of cellulose played a dominant role, with ethyl levulinate (EL) as the main liquid product. In addition, organic esters and furan derivatives were the main small molecules in the liquid, and diethoxymethane and diethyl sulfate were considered as the main liquid by-products. On the basis, the kinetics of one-pot preparation of EL from cellulose was further investigated at a temperature range of 170–210 °C and an acid concentration range of 0.5–2.0 wt%. Artificial Neural Network (ANN) was employed to develop an approach for the evaluation of the process. A good agreement of the ANN model results and the experimental data was obtained, and the optimum reaction conditions for one-pot preparation of EL were temperature 188 °C, reaction time 30 min, acid concentration 1.2 wt%. Under the conditions, higher EL yield can be obtained, which was close to the ANN model result.
研究领域:
化学工程
·
生物化学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Shasha Qin,Jiamin Li,Zhiling Pan,Can Wang,Bing-Feng Zhang
Prodrug nanoassemblies fabricated by anticancer drug conjugates exhibited more advantages in controlled drug release and bioavailability and favorable antitumor efficacy. In this paper, lactobionic acid (LA) was connected with polyethylene glycol through amido linkages, and paclitaxel was joined with polyethylene glycol by means of ester bonds to form the prodrug copolymer LA-PEG-PTX. Then, LA-PEG-PTX was automatically assembled into LA-PEG-PTX nanoparticles (LPP NPs) by dialysis. The LPP NPs had a relatively uniform size of approximately 200 nm, a negative potential (−13.68 mV), and a spherical shape under TEM. The drug loading of LPP NPs was 3.91%, which was measured by HPLC. The in vitro release profile of LPP NPs exhibited a sustained release feature. The results of the pharmacokinetic test in rats showed that LPP NPs had higher T 1/2 and AUC values than the control group (free PTX) and a prolonged in vivo circulation time, thus increasing the bioavailability of PTX. Remarkably, the LPP NPs were absorbed into HepG2 cells after galactose-directed internalization and enhanced cytotoxicity. Consequently, LPP NPs displayed notable antitumor activity in Kunming mice with H22 hepatocellular carcinoma. Collectively, these findings suggested that paclitaxel prodrug-based self-assembled nanoparticles were a promising alternative for improving the bioavailability and antitumor effect of PTX.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Hongli Wu,Ting Huang,Fei Cao,Qiaogen Zou,Ping Wei,Pingkai Ouyang
Co-production of multi-products is one of the core principles of chemical industry, and it is also an important way to improve the atom economy. Herein, we proposed an approach to co-producing two valuable platform compounds, gluconic acid (GA) and 5-hydroxymethyl furfural (HMF), from sucrose by successive hydrolysis, oxidation and dehydration. In the enzymatic oxidation step, only glucose was oxidized to GA, meanwhile fructose was 100% retained. In the further biphasic dehydration system, only fructose was converted into HMF, whereas GA was maintained with over 95% recovery. After three reactions, the yields of HMF and GA were respectively 42.5% and 48% when the initial feedstock of sucrose was 200 g/L. Two products were easily separated because GA was completely existed in aqueous phase, and HMF was mainly in organic phase. In the whole process, only commercial enzymes and mineral acid were used instead of self-made catalysts.
研究领域:
生物化学
·
化工
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2014
·
IF 2.354
Yan Liang,Kui Yu,Jiexin Wang,Jianfeng Chen,Baochang Sun,Lei Shao
The effect of erythorbic acid (EA) on the aqueous formation of CdS quantum dots (QDs) at room temperature was explored in this work. Without N 2 protection, CdS QDs were synthesized in water by a one-pot non-hot-injection approach at room temperature. The Cd and S sources were CdCl 2 and Na 2 S, respectively, together with 3-mercapto-propionic acid (MPA) as the passivating ligand. The experimental results indicate that the use of the oxygen scavenger, i.e., EA, was critical for the formation of the CdS QDs with reasonably good optical properties, and it is also important to tune experimental parameters such as MPA-to-Cd molar ratios, pH, and reactant concentrations. The mechanism about the EA promoted formation of CdS QDs was discussed in terms of NMR, IR, in situ absorption, and photoemission studies. The very reason for the EA promoted formation of CdS QDs is due to its reducibility and passivation on the QD surface (particularly in alkaline environment). The present study suggests that the use of EA could be a practical means to enable the room-temperature formation of QDs in water.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2019
·
IF 2.584
Chen Liang,Yan Wang,Yangdong Hu,Lianying Wu,Weitao Zhang
Butyl levulinate (BL) is a versatile chemical utilized widely in food and chemical industries, the production of which by using cellulose in biomass resources is of great significance to its sustainable development. Traditional synthesis processes for n-butyl levulinate are confronted with various problems such as high cost of raw materials, difficulty in separating products, etc. In this paper, a novel process for the preparation of BL from cellulose is proposed. The process is composed of five main unit operations including fed-batch hydrolysis, decolorization, extraction, esterification and purification. A 171.63 g/L concentration of the intermediate levulinic acid was obtained at the fifth feeding through the fed-batch hydrolysis process. The resin-activated carbon secondary decolorization method was adopted to remove the soluble humin byproducts with an accumulative decolorization rate of 89%. In the extraction process, the product BL was chosen as the extractant to avoid the introduction of new impurities. After purification, the purity of the final product BL reaches up to 98 wt %. The proposed technique allows for cost-effective and eco-friendly production of BL from biomass resources.
研究领域:
生物化学工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Ruyun Lou,Weiting Yu,Yizhe Song,Ying Ren,Huizhen Zheng,Xin Guo,Yunfei Lin,Guoyu Pan,Xiuli Wang,Xiaojun Ma
Galactose moieties are covalently coupled with sodium alginate to enhance liver-specific functions in microcapsules owing to the specific interaction between the galactose moieties and the asialoglycoprotein receptors (ASGPRs) of hepatocytes. In this study, galactosylated alginate (L-NH 2 -OH-alginate) based microcapsules with desirable stability and a suitable 3D microenvironment are designed and fabricated for primary hepatocyte applications. The designed L-NH 2 -OH-alginate is fabricated via the application of ethylenediamine grafted lactobionic acid (L-NH 2 ) onto the hydroxyl groups of sodium alginate so that the negatively charged carboxyl groups intact in L-NH 2 -OH-alginate can effectively bond with Ca 2+ to form a stable three-dimensional gel network; a subsequent reaction with polycations forms a stable membrane of microcapsules. As a result, L-NH 2 -OH-alginate based microcapsules exhibit an excellent mechanical stability. Moreover, with a higher degree of substitution in L-NH 2 -OH-alginate (DS 0.41), the hepatocytes entrapped in L-NH 2 -OH-alginate microcapsules exhibit better viability and well-maintained liver-specific functions.
研究领域:
生物材料学
·
细胞工程
·
肝脏研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 8
·3398-3409
·
IF 3.9
Dianying Shen,Ge Song,Xinyang Sun,Fengjiao Fan,Jian Ding,Yong Fang,Peng Li
Cadmium (Cd) accumulation in rice protein has long been considered a significant threat to human health. In the present study, a costless and effective method based on gluconic acid (GA) rinsing of rice protein was developed to reduce Cd contamination in rice protein. Moreover, the effect of GA on the structural and functional properties of rice protein was evaluated. With liquid–solid ratio of 30 mL/g and oscillation time of 120 min, 96.0% and 93.6% of Cd were eliminated from rice protein-H and rice protein-L, respectively. In addition, the results of scanning electron microscopy, Fourier transform infrared, and sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses showed that GA treatment did not significantly change the structural properties of rice protein. However, GA treatment increased foaming properties, water holding capacity, and oil holding capacity of the rice protein, without affecting its further applicability. Thus, the proposed GA rinsing method can be considered a green and efficient strategy to solve the issue brought by Cd residual contamination in rice protein. Practical Application Given the advantages of green and efficient agriculture, gluconic acid (GA) has emerged as a powerful strategy for removing the Cd from rice protein. The method developed herein showed great potentials for applications in the manufacture of rice-based products.
研究领域:
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2023
·Vol. 16
·期 8
·10748-10755
·
IF 9.9
Li Junhua,Jiang Yi,Zhang Xu,Huo Yidan,Du Fanglin,Tuo Yongxiao,Guo Zhiyan,Chen Dawei,Chen Shenghua
The electrooxidation of the alcohol and aldehyde molecules instead of water coupled with H 2 production has been proven to be effective for producing high-value fine chemicals under alkaline conditions. It is also noteworthy that under acidic conditions, the stability of non-noble metal water oxidation catalysts remains a great challenge due to the lattice oxygen mechanism. Hence, we coupled the biomass-derived glucose oxidation for high-value D-glucaric acid (GRA) with ultra-durable hydrogen in acid solution over a Yb-MnO 2 catalyst. The Mn 3+ regulated by Yb atoms doped in MnO 2 can effectively optimize the adsorption and desorption processes of the alcohol and aldehyde group and improve the intrinsic activity but cannot for H 2 O. The catalyst exhibited extremely high activity and stability after 50 h for glucose oxidation, inhibiting the lattice oxygen process and MnO 4− formation, while the activity was quickly lost within 0.5 h for water oxidation. Density functional theory (DFT) calculations further demonstrated that glucose oxidation reaction proceeds preferentially due to the oxidation of aldehyde group with lower adsorption-free energy (−0.4 eV) than water (Δ G > 0 eV), avoiding the lattice oxygen mechanism. This work suggests that biomass-derived glucose oxidation not only provides a cost-effective approach for high-value chemicals, but also shows an extremely potential as an alternative to acidic oxygen evolution reaction (OER) for ultradurable H 2 production.
研究领域:
生物化学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）