Fermentation-Basel
·
2025
·Vol. 11
·期 3
·119
·
IF 3.3
Liang Wu,Yong Xu
Bacillus subtilis(B. subtilis) has booming application prospects in the feed industry as a value-added probiotic. Considering the intermediate and end saccharides of glucan and xylan digestion during lignocellulose biorefinery processing, the various digested saccharides were detected to disclose the bio-uptake and fermenting characteristics by solo or co-fermentation, especially with respect to five monosaccharides, cellobiose, and functional food additive—xylo-oligosaccharides (XOS).B. subtiliscan directly ferment XOS without xylose, achieving a cell yield of 0.71 g/g, while it hardly utilizes cellobiose except glucose co-fermentation. With a 5 g/L glucose addition and co-fermentation, bacterial cell yield was improved to 0.70 g/g. In total,B. subtilishad a 0.56–0.90 g/g cell yield from corncob-digested saccharides and the highest saccharide-uptaking rate of 98%. This study provides critical insights and experimental data to advance lignocellulosic biorefinery technologies, particularly for probiotics production and simplification of enzymatic hydrolysis, as well as the potential application in high value-added XOS biopurification.
研究领域:
生物工程
·
微生物学
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产品关联
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Chuyin Qiu,Weiting He,Yu Li,Feng Jiang,Yang Pan,Meihui Zhang,Daying Lin,Kaili Zhang,Yanduo Yang,Wen Wang,Pei Hua
Chlorine disinfection is widely applied in drinking water treatment plant to inactivate pathogens in drinking water , but it unintentionally reacts with organic matter present in source waters and generates halogenated disinfection byproducts (DBPs). Sugar is one of the most commonly used seasoning in our diet. The addition of sugar could significantly improve the taste of the beverages; however, the effects of sugar on DBP formation and transformation remain unknown. In this study, the effects of sugar type and dose on the halogenated DBP formation in chlorinated boiled real tap water were evaluated during making hot beverages. We found that sugar can react with chlorine residual in tap water and generate halogenated DBPs. As the most commonly used table sugar, the addition of sucrose in the water sample at 100 or 500 mg/L as C could increase the level of total organic halogen (TOX) by ∼35%, when compared with the boiled tap water sample without sugar addition. In addition, fifteen reported and new polar brominated and chlorinated DBPs were detected and proposed from the reaction between chlorine and sucrose; accordingly, the corresponding transformation pathways were also proposed. Moreover, the DBP formation in the chlorinated boiled real tap water samples with the addition of xylose , glucose, sucrose, maltose and lactose were also investigated. By comparing with the TOX levels in the water samples with different sugar addition and their calculated TOX risk indexes, it was suggested that applying xylose as a sweetener in beverages could not only obtain a relatively high sweetness but also minimize the adverse effect inducing by halogenated DBPs during making hot beverages.
研究领域:
水处理
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环境化学
·
食品科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Qianying Sheng,Xindai Su,Xiuling Li,Yanxiong Ke,Xinmiao Liang
A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides.
研究领域:
分析化学
·
色谱学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 11
·4927-4943
·
IF 3.53
Deng Pengjun,Meng Chunyu,Wu Yan,Xu Jie,Tang Xiaoqing,Zhang Xuecheng,Xiao Yazhong,Wang Xiaotang,Fang Zemin,Fang Wei
A novel β -glucosidase, BglD1 with high β -galactosidase and transglycosidation activities, was screened and cloned from the deep-sea bacterium Bacillus sp. D1. BglD1 exhibited the maximal β -glucosidase and β -galactosidase activities at 55–60 °C and pH 5.5–6.0. The enzyme maintained approximately 50% of its original activity at 35 °C and pH 6.0 after 120-h incubation. When applied to synthesize galacto-oligosaccharides (GOS), BglD1 generated 118.3 g/L GOS (33.8% (w/w)) from 350 g/L lactose, with trisaccharide Gal- β (1 → 3)-Lac and disaccharide Gal- β (1 → 4)-Gal as the main components. Furthermore, BglD1 could hydrolyze lactose in milk and produce GOS simultaneously. Using milk as the substrate, BglD1 hydrolyzed 88.5% lactose and produced 3.3 g/L GOS after incubation at 30 °C for 1 h. To improve the transglycosidation activity, a mutant BglD1:E224T was generated based on the semi-rational design. The GOS yield of BglD1:E224T was 11.5% higher than that of BglD1 when using lactose solution as the substrate. Thus, BglD1 and the mutant could be used as beneficial alternatives of the existing β -galactosidases for the production of GOS.
研究领域:
微生物学
·
酶工程
·
食品科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Juncheng Chen,Lin Li,Xia Zhang,Yuan Zhang,Qingsong Zheng,Meijuan Lan,Bing Li
In this study, an acid polysaccharide (AABP-1B) was extracted from the rhizome of Anemarrhena asphodeloides Bunge and purified using 60 % alcohol precipitation and DEAE-52 cellulose. The molecular weight of AABP-1B was 105 kDa, and it consisted of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara) in a ratio of 6.3:1.3:1.1:0.2:0.4:0.7. Methylation and NMR analyses revealed that the backbone of AABP-1 consists of 4)-β-D-Man p -(1 and 4)-2- O -acetyl-β-D-Man p -(1. In addition, the biological activity assays showed that AABP-1B not only displays potential antioxidant activity but also exhibits the α-glucosidase and α-amylase inhibitory effect. Moreover, AABP-1B enhanced glucose consumption and glycogen synthesis in insulin-resistant (IR) HepG2 cells. These results suggest that AABP-1B has potential hypoglycemic activity.
研究领域:
药用植物学
·
生物化学
·
分子生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Baojun Feng,Hong Liao,Wenjun Ying,Junhua Zhang,Zhangjing Chen
Short-chain xylo-oligosaccharides (XOS) such as xylobiose (X2) and xylotriose (X3) have higher biological activities. Therefore, it is interesting to produce highly active XOS enriched with X2 and X3. In this work, pH-controlled lactic acid (LA) hydrolysis was used to produce XOS from xylan in moso bamboo and xylanase was used to convert high DP XOS into low DP XOS to increase the percentage of X2 + X3 in XOS. A 33.1 % XOS yield was obtained from 2 % LA hydrolysis (pH = 3.2). After xylanase hydrolysis of the LA hydrolysate, the total XOS yield reached 64.1 %, with X2 + X3 yield reaching 58.4 %. The percentage of X2 + X3 increased from 52.3 % to a high level of 91.0 %. The deep eutectic solvent pretreatment removed 87.2 % lignin from the residue and the glucose yield of the delignified residue hydrolyzed by cellulase was 96.9 %. The results suggested that the integrated process of LA and xylanase hydrolysis could effectively produce X2 + X3 from xylan in moso bamboo.
研究领域:
生物化学
·
可再生资源与环境科学
✓ 已验证
DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 3
·162
·
IF 5.118
Xiang Shi,Ruowei Guan,Lutan Zhou,Zhichuang Zuo,Xuelin Tao,Pin Wang,Yanrong Zhou,Ronghua Yin,Longyan Zhao,Na Gao,Jinhua Zhao
Unique fucosylated glycosaminoglycans (FG) have attracted increasing attention for various bioactivities. However, the precise structures of FGs usually vary in a species-specific manner. In this study, HfFG was isolated fromHolothuria floridanaand purified by anion exchange chromatography with the yield of ~0.9%. HfFG was composed of GlcA, GalNAc and Fuc, its molecular weight was 47.3 kDa, and the -OSO3−/-COO−molar ratio was 3.756. HfFG was depolymerized by a partial deacetylation–deaminative cleavage method to obtain the low-molecular-weight HfFG (dHfFG). Three oligosaccharide fragments (Fr-1, Fr-2, Fr-3) with different molecular weights were isolated from the dHfFG, and their structures were revealed by 1D and 2D NMR spectroscopy. HfFG should be composed of repeating trisaccharide units -{(L-FucS-α1,3-)d-GlcA-β1,3-d-GalNAc4S6S-β1,4-}-, in which sulfated fucose (FucS) includes Fuc2S4S, Fuc3S4Sand Fuc4Sresidues linked to O-3 of GlcA in a ratio of 45:35:20. Furthermore, the heparanase inhibitory activities of native HfFG and oligosaccharide fragments (Fr-1, Fr-2, Fr-3) were evaluated. The native HfFG and its oligosaccharides exhibited heparanase inhibitory activities, and the activities increased with the increase of molecular weight. Additionally, structural characteristics such as sulfation patterns, the terminal structure of oligosaccharides and the presence of fucosyl branches may be important factors affecting heparanase inhibiting activity.Keywords:Holothuria floridana;fucosylated glycosaminoglycans;oligosaccharide;chemical structure;heparanase
研究领域:
海洋生物学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jianping Li,Xionghui Ma,Mengxi Li,Yun Zhang
A novel glycoprotein imprinting strategy was proposed and was applied to the detection of the carbohydrate antigen 19-9. The glycosylated complex of glycoprotein was used as template to construct a glycosyl imprinted sensor. The eluted imprinted cavities showed good affinity for template glycosyls and glycoproteins carrying template glycosyl. The effect of template saccharide structure on glycosyl imprinted sensors is further discussed. More complex template structures can lead to better sensor performance including selectivity and sensitivity. As a result, the polysaccharide imprinted sensor showed preeminent linear response to CA19-9 in the range of 0.1–5 U/mL, with a detection limit of 0.028 U/mL (3δ/K), while the linear of the monosaccharide imprinted sensor was 1–60 U/mL and the detection limit was 0.17 U/mL. The complex structure on the template surface provides more possibilities for the recognition of the template molecules, consequently, led to the significant anti-interference capability of the polysaccharide imprinted sensor. Furthermore, recoveries ranging from 93.0% to 103.5% were achieved when human serum samples were assayed using the polysaccharide imprinted sensor.
研究领域:
生物化学
·
医学诊断
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yanyun Zhu,Yunyun Zheng,Bingge Liu,Kouadio Jean Eric-Parfait Kouame,Ebenezer O. Falade,Jianchu Chen,Xingqian Ye
Monosaccharide composition and glycosidic linkages analysis are essential for the structural characterization and biological activity research of polysaccharides. To simplify the analysis steps and improve detection efficiency, this study developed monosaccharide compositions and glycosidic linkages detection methods based on UPLC-QqQ-MS/MS, and established a plant polysaccharide glycosidic linkages library. Furthermore, the detailed analysis process of monosaccharide compositions and glycosidic linkages was presented through a plant polysaccharide (Chinese bayberry wine polysaccharide, CPW) example. The results showed that the monosaccharide analysis method could identify and quantify 20 monosaccharides within 13 min by 6 chromatograms. By this method, 7 monosaccharides were detected in CPW, which was consistent with previous results obtained using ion chromatography. Meanwhile, the UPLC-QqQ-MS/MS based method could analyze 112 glycosidic linkages of 9 plant monosaccharides within 30 min, and the results were distributed on 11 chromatograms. According to the approach, 44 different glycosidic linkages were identified in CPW. Based on Congo red assay and molecular weight as well as our findings of monosaccharide composition and glycosidic linkages, we proposed that CPW may consist of two distinct sugar chains: One composed of 1–2,4-Rha, 1–3,4-GalA, and 1-F-Ara, and the other composed of 1–3,4-Gal, 1–4-Gal, 1–3,4,6-Gal, and 1-F-Ara.
研究领域:
分析化学
·
植物学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
·
2022
·
IF 5.089
Hengtao Tang,Zheng Zhou,Zhi Chen,Xin Ju,Liangzhi Li
Monosaccharides from biomass can be used as cost-effective raw materials to produce rare sugars which are valuable sweeteners. Enzymes play a pivotal role in such biomass biorefinery processes to develop novel synthetic routes, and improve the environmental friendliness of current routes. Here we propose a proof of concept biorefinery process to prepare useful rare sugars of D-xylulose and D-ribulose from D-xylose which was abundant in biomass. Ribose-5-phosphate isomerase B from Ochrobactrum sp. (OsRpiB) with a unique substrate scope was engineered to intensify the activity of transforming D-xylose to D-xylulose. A double mutant R94N/N137Q with 80% higher catalytic efficiency was identified, and kinetic and molecular dynamic studies were conducted to explore the mechanism underneath. The results revealed a correlation between enhancing stability and activity simultaneously. Then a one-pot enzyme cascade was constructed with OsRpiB and D-tagatose-3-epimerase from Pseudomonas cichorii and transformed D-xylulose further to D-ribulose. The conversions of D-xylose and D-xylulose in cascade reaction were 1.80-fold and 1.83-fold of that in separate reactions with the aid of process parameter adjustment. The presented work is the first reported enzyme cascade converting D-xylose to D-ribulose, and proves the promising role of monosaccharide isomerase platforms in the refinery of biomass.
研究领域:
生物化学
·
酶工程
·
生物质精炼
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产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 12
·期 12
·5451-5464
·
IF 5.396
Zu-Man Dou,Chun Chen,Qiang Huang,Xiong Fu
A novel heteropolysaccharide fraction (BBP-24-3) with a relative molecular weight of 145.1 kDa was isolated from blackberry fruits. The BBP-24-3 was mainly composed of arabinose, glucose, and galacturonic acid with a ratio of 5.30 : 3.60 : 91.10 mol%. Structural analysis showed that BBP-24-3 possessed a 1,6-linked β-D-Glcp, 1, 2, 3, 5 linked α-L-Araf, and 1, 4 linked α-D-GalpA backbone with branches substituted at the C-2 and C-5 positions of arabinose units, which included 1, 2, 3, 4 linked β-D-Glcp and T-linked β-D-GalpA. The conformation analysis indicated that BBP-24-3 exhibited a solid spherical structure with a uniform distribution in 0.1 M NaCl solution. The BBP-24-3 exhibited excellent α-glucosidase inhibitory activity with an IC50 value of 3.70 mg mL−1, which was due to the structural change, including α-helix and random coil of α-glucosidase caused by BBP-24-3. The current work suggests the potential utilization of BBP-24-3 as an α-glucosidase inhibitor in healthy food for reducing the postprandial blood glucose level.
研究领域:
食品科学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Qi-Wu Zhong,Tao-Shun Zhou,Wen-Hui Qiu,Ya-Kun Wang,Qiao-Li Xu,Song-Ze Ke,Si-Jia Wang,Wei-Hua Jin,Jian-Wei Chen,Hua-Wei Zhang,Bin Wei,Hong Wang
The brown seaweed Undaria pinnatifida polysaccharides show various biological activities, but their hypoglycemic activity and the underlying mechanism remain unclear. Here, three fractions of sulfated polysaccharides Up-3, Up-4, and Up-5 were prepared by microwave-assisted extraction from U. pinnatifida . In vitro assays demonstrated that Up-3 and Up-4 had strong α-glucosidase inhibitory activity, and Up-3, Up-4, and Up-5 could improve the glucose uptake in insulin-resistant HepG2 cells without affecting their viability. In vivo studies indicated Up-3 and Up-4 markedly reduced postprandial blood glucose levels. Up-U (a mixture of Up-3, Up-4, and Up-5), reduced fasting blood glucose levels, increased glucose tolerance and alleviated insulin resistance in HFD/STZ-induced hyperglycemic mice. Histopathological observation and hepatic glycogen measurement showed that Up-U alleviated the damage of the pancreas islet cell, reduced hepatic steatosis, and promoted hepatic glycogen synthesis. These findings suggest that Up-U could alleviate postprandial and HFD/STZ-induced hyperglycemia and was a potential agent for diabetes treatment.
研究领域:
海洋生物学
·
药理学
·
糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）