INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Sihui Xiong,Ming Chen,Xun Pei,Jinjing Yin,Hélder Oliveira,Nuno Mateus,Shuxin Ye,Muci Wu,Rui Zhang,Jingren He
Purple rice ( Oryza sativa L.) is a rich in endogenous phenolics and proteins. The naturally occurring interactions between phenolic compounds and proteins have been shown to have beneficial effects on human health. In this study, four protein fractions of purple rice (albumin, prolamin, globulin, and glutelin) were extracted, and both protein-free and protein-bound phenolics (PFP and PBP) were isolated from each protein fraction. The major phenolics compounds identified in different protein fraction included protocatechuic acid, vanillic acid, and ferulic acid. Additionally, the PFP in the albumin fraction exhibited the highest number of anthocyanin glycosides (7 types) among all phenolic compounds, while the remaining compounds were identified only as cornflower-3-glucoside and paeoniflorin-3-glucoside. Moreover, the in vitro antioxidant activity and cancer cell inhibitory effects of PFP and PBP in various protein fraction were investigated using chemiluminescence and cellular assays. The results demonstrated that the inhibitory effect of H₂O₂ was more pronounced than that of other free radicals (O₂ − and OH − ), with albumin and prolamin exhibiting heightened antioxidant activities. Notably, the PBP in various protein fractions showed a higher antiproliferative capacity than their corresponding PFP, indicating a potential synergistic effect of protein-phenolic interactions that differed between the two cell lines, MKN-28 and HT-29.
研究领域:
食品科学
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分子生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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2025
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IF 2.7
Zhenchao Shao,Shuangshuang Di,Jie Lian,Honggang Nie
Zhongzi purple rice is recognized as a nutritionally superior whole-grain variety, containing higher levels of protein, iron, dietary fiber, and vitamin B6 compared to conventional rice. While the nutritional profile of Zhongzi purple rice is well-established, the spatial distribution and structural specificity of its lipid components, especially germ-specific triacylglycerols (TAGs), remain poorly characterized. This study employs a multimodal mass spectrometric strategy to investigate the lipidomic uniqueness of the Zhongzi purple rice. The strategy combines matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) for spatial analysis, high-resolution mass spectrometry (HRMS) for primary analysis, and high-resolution tandem mass spectrometry with atmospheric pressure chemical ionization (APCI-HRMS/MS) for secondary analysis. MALDI-MSI identified seven metabolites in Zhongzi purple rice that exhibited germ-specific accumulation. In contrast, these metabolites showed markedly reduced signal intensities and lacked structured spatial distribution in conventional purple, black, and brown rice. Using a combination of lipidomic database interrogation, HRMS molecular formula validation, and APCI-HRMS/MS structural elucidation, we identified these metabolites as unsaturated fatty acid-enriched TAGs. Specifically, they were TAG(16:0/16:0/18:2), TAG(16:0/16:0/18:1), TAG(16:0/18:1/18:3), TAG(16:0/18:1/18:2), TAG(16:0/18:1/18:1), TAG(18:1/18:2/18:2), and TAG(18:1/18:1/18:2). The high unsaturation of these TAGs contributes to improved lipid hydrolysis efficiency, metabolic health benefits, and oxidative stability. These properties highlight Zhongzi purple rice as a potential functional food source. Due to their germ-specific accumulation, these compounds can act as potential biomarkers for authenticating Zhongzi purple rice and detecting adulterants. This provides a practical solution to commercial challenges in the industry. This study pushes the boundaries of cereal lipidomics through the integration of spatially resolved imaging and structural validation. The results shed light on the role of lipids in driving the nutritional and metabolic benefits of whole grains.
研究领域:
食品科学
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脂质组学
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分析化学
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营养学
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分子生物学
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代谢组学
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作物品质研究
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Xianbao Li,Binxu Li,Natalia S. Podio,Xiao-Yin Wang,Suhang Jiang,Shuyan Xu,Xuemei Qiu,Zheng Zeng,Wei Gong,Shirui Wang,Er Sheng Gong
Phenolics in three purple rice varieties were qualitatively and quantitatively analyzed using untargeted metabolomics, and their antioxidant activities were also investigated. Jixuenuo showed the highest antioxidant activity and phenolic content among the three varieties. Ninety-three phenolics across four categories (flavonoids, phenolic acids, stilbenes, and lignans) were identified, with Jixuenuo showing the highest anthocyanin content. Cyanidin-3 -O- glucoside (C3G) accounted for over 90% of total anthocyanin content. The volcano plot analysis identified 53 differential phenolics, 22 of which were classified as major. KEGG pathway analysis revealed significant enrichment in flavonoids biosynthesis pathways. Jixuenuo showed significantly enhanced biosynthesis in the pathways for cyanidin, dihydromyricetin, epicatechin, C3G, myricetin, and rutin. Correlation analysis indicated that these flavonoids were significantly positively correlated with antioxidant activity and total phenolic content. These findings suggest that specific flavonoids in Jixuenuo may serve as markers for evaluating the antioxidant potential of purple rice.
研究领域:
食品科学
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营养学
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代谢组学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）