Frontiers in Pharmacology
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2020
·
IF 4.225
Li Qianqian,Zhou Jia,Zhang Dingyi,Zhang Xiafeng,Xu Zhenghao,Wu Dehong
Glucocorticoids (GCs) are commonly used to treat systemic lupus erythematosus (SLE). Unfortunately, excessive GCs can induce many side effects associated with disordered fatty acid (FA) metabolism. Although an increased level of total FA has been found after GCs treatment, it is not clear whether all FA species increased or only certain FA species were altered. A gas chromatography–mass spectrometry-based FA profiling approach was performed to reveal the alterations of FA species in SLE model mice (MRL/lpr) after treatment with 5 mg/kg of prednisone. The study showed a distinct FA profile in MRL/lpr mice compared to the controls, mainly manifested by elevated polyunsaturated FAs (arachidonate, docosahexaenoate, etc.), which are related to the inflammatory state; and altered (product FA/precursor FA) ratios representing the estimated activities of FA desaturase and elongase (higher activities of multiple elongases, △4 desaturase, △5 desaturase, △6 desaturase, and lower activity of △8 desaturase). Treatment with 5 mg/kg of prednisone decreased the total level of n-6 polyunsaturated FA in MRL/lpr mice; in particular, the level of arachidonate and estimated activity of △5 desaturase were reduced to the control level. Moreover, prednisone induced additional perturbations in FAs, including not only saturated FAs, but also monounsaturated FAs and n-3 polyunsaturated FAs, indicating that there was a strong effect of prednisone on FA metabolism. These results may be valuable for further studies of the side effects of GCs treatment.
研究领域:
免疫学
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药理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 5
·415
·
IF 5.581
Jida Zhang,Lu Lu,Xiaoyu Tian,Kaili Wang,Guanqun Xie,Haichang Li,Chengping Wen,Changfeng Hu
Cardiac involvement, displayed as premature cardiovascular disease (CVD), is one of common clinical symptoms of patients with systemic lupus erythematosus (SLE), contributing to mortality of the disease. The precise underlying pathological mechanism(s) for the cardiac involvement in lupus remains poorly understood. Lipids and their metabolites are directly involved in atherosclerosis development, oxidative stress, and inflammation, which are closely related to the development of CVD. In the study, shotgun lipidomics was exploited to quantitatively analyze cellular lipidomes in the cardiac tissue of MRL/lpr mice at two different time points (i.e., pre-lupus and lupus state) with/without treatment with glucocorticoids (GCs). Urine protein, spleen index, and renal histopathological evaluation of the mice were also performed for assessment of SLE onset and/or outcome. Lipidomics analysis revealed that the deposition of cholesterol and the aberrant metabolism of lipids caused by the increased energy metabolism and the enhanced activation of phospholipases, both of which were originally induced by inflammation, were already present in cardiac tissues from lupus-prone mice even at pre-lupus state. These lipid alterations could further induce inflammation and autoimmune responses, accelerating the process of CVD. In addition, the present study also demonstrated that GCs therapy could not only delay the progression of SLE, but also partially corrected these alterations of lipid species in cardiac tissue due to their anti-inflammatory effect. Thus, the medications with better anti-inflammatory effect might be a useful therapeutic method for premature CVD of SLE.Keywords:systemic lupus erythematosus;lipidomics;inflammation;premature cardiovascular disease;glucocorticoids
研究领域:
免疫学
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心脏病学
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药理学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Yuhao Huang,Chen Chen,Chenjie Chang,Zhiyuan Cheng,Yang Liu,Xuehua Wang,Cheng Chen,Xiaoyi Lv
As artificial intelligence technology gains widespread adoption in biomedicine, the exploration of integrating biofluidic Raman spectroscopy for enhanced disease diagnosis opens up new prospects for the practical application of Raman spectroscopy in clinical settings. However, for systemic lupus erythematosus (SLE), origin Raman spectral data (ORS) have relatively weak signals, making it challenging to obtain ideal classification results. Although the surface enhancement technique can enhance the scattering signal of Raman spectroscopic data, the sensitivity of the SERS substrate to airborne impurities and the inhomogeneous distribution of hotspots degrade part of the signal. To fully utilize both kinds of data, this paper proposes a two-branch residual-attention network (DBRAN) fusion technique, which allows the ORS to complement the degraded portion and thus improve the model's classification accuracy. The features are extracted using the residual module, which retains the original features while extracting the deep features. At the same time, the study incorporates the attention module in both the upper and lower branches to handle the weight allocation of the two modal features more efficiently. The experimental results demonstrate that both the low-level fusion method and the intermediate-level fusion method can significantly improve the diagnostic accuracy of SLE disease classification compared with a single modality, in which the intermediate-level fusion of DBRAN achieves 100% classification accuracy, sensitivity, and specificity. The accuracy is improved by 10% and 7% compared with the ORS unimodal and the SERS unimodal modalities, respectively. The experiment, by fusing the multimodal spectral, realized rapid diagnosis of SLE disease by fusing multimodal spectral data, which provides a reference idea in the field of Raman spectroscopy and can be further promoted to clinical practical applications in the future.
研究领域:
生物医学工程
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光谱学
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人工智能在医学中的应用
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产品关联
DOI
PubMed
高影响因子
MOLECULAR IMMUNOLOGY
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2023
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IF 3.6
Wei Zhong,Leheng Feng,Wei Tian,Hongbo Qu,Haibo Xu,Ke Ning,Li Liu,Wei Liu,Xiaowei Gong,Hong Chen
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease . This study aimed to investigate the role of SMAD specific E3 ubiquitin protein ligase 1 (SMURF1) in the Th17 and Th17.1 differentiation and Treg/Th17 imbalance, which are major factors contributing to the pathogenesis of SLE. SLE patients and healthy individuals were recruited to detect the SMURF1 levels in naïve CD4 + cells from peripheral blood. Purified and expanded naïve CD4 + T cells were employed to evaluate the effects of SMURF1 on Th17 and Th17.1 polarization in vitro. MRL/lpr lupus model was employed to explore the disease phenotype as well as Treg/Th17 balance in vivo. The results showed that SMURF1 was down-regulated in naïve CD4 + T cells in peripheral blood of patients with SLE and in spleen of MRL/lpr mice. SMURF1 overexpression suppressed the polarization of naïve CD4 + T cells toward Th17 and Th17.1 phenotype and down-regulated the expression of retinoid-related orphan receptor-gammat (RORγt). Subsequently, SMURF1 down-regulation aggravated the disease phenotype, inflammation, and the Treg/Th17 imbalance in MRL/lpr mice. Furthermore, we found that SMURF overexpression promoted the ubiquitination and decreases the stability of RORγt. In conclusion, SMURF1 inhibited the polarization of Th17 and Th17.1 cells and improved the Treg/Th17 imbalance in SLE, which was mediated as least partly by the ubiquitination of RORγt.
研究领域:
免疫学
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自身免疫性疾病
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分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Xiaoxia Chen,Jingjing Xu,Min He,Yiting Chen,Wenqi Tang,Songmin Chen,Xiyao Zhang,Wenhui Xu,Ziluan Zhu,Qiong Hu,Li Niu
Anti-dsDNA antibodies are of great value in the diagnosis of systemic lupus erythematosus (SLE). Here, we report the development of an amplification-free electrochemical method for the signal-on assay of anti-dsDNA antibodies at low abundance levels, taking advantage of the silver deposition evoked by the target’s glycan chains. Briefly, the method involves (i) the capture of anti-dsDNA antibodies by the double-stranded DNA (dsDNA) recognition probes, (ii) the cleavage of the diol sites in glycan chains into aldehyde groups by periodate oxidation, (iii) the reduction of silver ions into the metallic deposits, and (iv) Ag/AgX solid-state voltammetric stripping analysis. As each anti-dsDNA antibody has two N-linked biantennary glycan chains, the capture of an anti-dsDNA antibody can bring about the reduction of tens of silver ions into metallic silver. Thus, the glycan-evoked silver deposition can enable an amplification-free assay of anti-dsDNA antibodies with high sensitivity. Under optimal conditions, the method permitted the signal-on assay of anti-dsDNA antibodies down to 0.13 IU/mL (∼31.2 ng/mL), with the assay time as short as ∼69 min (from sample addition to signal readout). Moreover, it can selectively discriminate anti-dsDNA antibodies from other glycoproteins, and the voltammetric responses toward the assay of serum samples from patients with SLE had a good quantitative correlation with the clinical chemiluminescent immunoassay. By virtue of the simple operation, low cost, mild reaction conditions, and rapid readout, the glycan-evoked silver deposition shows good promise in the point-of-care electrochemical assay of anti-dsDNA antibodies at low abundance levels.
研究领域:
免疫学
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临床检验
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分子诊断
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电化学分析
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自身免疫病
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转化医学
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生物传感器
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产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 7
·2004184
·
IF 16.806
Cheng Zhao,Lijun Cai,Min Nie,Luoran Shang,Yongan Wang,Yuanjin Zhao
Oral drug administration has an important role in medical treatment. Attempts to develop drug microcarriers with desired features for extended duration and improved absorption is highly sought. Herein, inspired by the physical phenomenon of the Cheerios effect, a novel microfluidic electrospray microbubble carrier is presented that can suspend and actively adhere to the stomach for durable oral delivery. Compared with conventional fabrication methods, the present strategy shows stability and controllability of the product. Benefiting from their uniform hollow structure, the resultant microbubbles present the same behavior of the Cheerios and can float in the gastric juice, adhere and remain to the stomach wall, which thus enhance the duration and absorption of the loaded drugs. Based on these, it is demonstrated as a proof of concept that the dexamethasone-loaded hollow microbubbles can be applied to oral administration and remain suspended and adhered to the stomach of murine for more than 1 d, showing good therapeutic effect in treating lupus erythematosus. Thus, it is believed that the microbubbles floating system will find important values in long-term oral administration.
研究领域:
药学
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药物递送系统
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微流控技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Xuehua Wang,Junwei Hou,Chen Chen,Zhenhong Jia,Enguang Zuo,Chenjie Chang,Yuhao Huang,Cheng Chen,Xiaoyi Lv
Systemic lupus erythematosus (SLE) is an autoimmune disease with multiple symptoms, and its rapid screening is the research focus of surface-enhanced Raman scattering (SERS) technology. In this study, gold@silver-porous silicon (Au@Ag-PSi) composite substrates were synthesized by electrochemical etching and in-situ reduction methods, which showed excellent sensitivity and accuracy in the detection of rhodamine 6G (R6G) and serum from SLE patients. SERS technology was combined with deep learning algorithms to model serum features using selected CNN, AlexNet, and RF models. 92 % accuracy was achieved in classifying SLE patients by CNN models, and the reliability of these models in accurately identifying sera was verified by ROC curve analysis. This study highlights the great potential of Au@Ag-PSi substrate in SERS detection and introduces a novel deep learning approach for SERS for accurate screening of SLE. The proposed method and composite substrate provide significant value for rapid, accurate, and noninvasive SLE screening and provide insights into SERS-based diagnostic techniques.
研究领域:
医学诊断
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生物技术
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人工智能
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2021
·
IF 2.351
Chijian Zhang,Yongjie Lu,Yong Ai,Xian Xu,Siyang Zhu,Bing Zhang,Minghui Tang,Lanyue Zhang,Tinggang He
Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.
研究领域:
药学
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皮肤病学
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
Advanced Optical Materials
·
2022
·Vol. 11
·期 5
·2202248
·
IF 10.05
Neng Hu,Di Gao,Xin Cai,Lin Lei,Weijia Wang,Huiqing Fan,Peter Müller-Buschbaum,Qi Zhong
Inspired by the lady costume from Tang Dynasty, printable tattoos are prepared from hybrid nanogels to monitor ultraviolet (UV) radiation from sunshine. The hybrid poly(di(ethyleneglycol) methylether methacrylate- co -poly(ethylene glycol) methylethermethacrylate) nanogels containing graphitic carbon nitride (g-C 3 N 4 ) nanosheets, abbreviated as hybrid P(MA- co -MA 300 )/g-C 3 N 4 nanogels, are prepared by precipitation polymerization. Due to the excellent photocatalysis of the g-C 3 N 4 nanosheets, the dyed hybrid nanogels can be rapidly photodegraded under sunshine. Based on the linear correlation between the discoloration of dyed hybrid nanogels and the dose of solar radiation, the UV radiation from sunshine can be simply monitored by checking the color in real-time. By applying different types of dyes, the hybrid nanogels even can be used to trace the dose for the synthesis of vitamin D, induction of erythema and risk of skin cancer simultaneously. Favored by the stickiness of acrylate-based polymers, the hybrid nanogels can be printed to the body as tattoos to realize decoration and UV detection. By simply switching the template for printing, the tattoo patterns can be easily changed according to personal interests and applied position on the human body. Therefore, the present hybrid nanogels can be a promising candidate to monitor the solar radiation dose and decorate the human body.
研究领域:
材料科学
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纳米技术
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光催化
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生物医学工程
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化妆品科学
✓ 已验证
产品关联
DOI
高影响因子
Nano Today
·
2022
·
IF 18.962
Haofang Zhu,Bin Kong,Min Nie,Cheng Zhao,Rui Liu,Ying Xie,Yuanjin Zhao,Lingyun Sun
Mesenchymal stem cells (MSCs) transplantation is an emerging and attractive therapy for immune dysregulation diseases like systemic lupus erythematosus (SLE). However, its application is hindered by short in vivo persistence and poor cellular survival after injection. Here, inspired by the native extracellular matrix (ECM) composition , we presented a novel peptide dendrimer-based microgel encapsulated with MSCs via a microfluidic approach for SLE treatment. By varying microfluidic parameters, monodisperse microgels with tunable diameters, porous microstructures, mechanical stiffness, and immune-isolation potential could be precisely achieved. As the water polymerization environment and ECM like components, the resultant microgels exhibited minimal toxicity in vivo and in vitro. We have also demonstrated that the MSCs encapsulated microgels could significantly increase the cellular bioactivity , enhance the immunomodulatory function, and attenuate disease symptoms in MRL/lpr mice. These results indicate the bioinspired microgels with MSCs encapsulation have potential clinical values in the treatment of SLE and other immune dysregulation diseases.
研究领域:
生物材料
·
干细胞治疗
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免疫学
·
生物医学工程
✓ 已验证
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 32
·期 13
·2110746
·
IF 18.808
Lu Fan,Xiaoxuan Zhang,Min Nie,Ye Xu,Yuetong Wang,Luoran Shang,Yongxiang Zhao,Yuanjin Zhao
Microneedle (MN) patches are promising stratagems for transdermal drug delivery owing to its minimal invasiveness and portability. Particularly, separable MN patches attract more attention for durable and long-term drug delivery. Here, a new strategy for designing controllably separable MN patches by integrating photothermal responsive phase-changing microspheres (MPs) into conical hydrogel MNs, is presented. Such MPs consist of near-infrared (NIR)-responsive black-phosphorus (BP) and phase-changing gelatin (GT), and are fabricated using a simple capillary microfluidic device. When the composite MN patch is exposed to NIR, the contained BP/GT MPs can transform light into heat, increase local temperature, turn into a liquid state, and form cavities in MNs, resulting in the convenient detachment of the backing plate and the needles by shear force. Subsequently, the embedded needles can sustainably release the drug. In addition to intelligently controlling the detachment of MNs, the BP/GT MPs can load macromolecular drugs and release them in response to NIR stimulation. Results further demonstrated that such controllably separable MN patch can effectively load and release two drugs to ameliorate disease progression for systemic lupus erythematosus treatment. These features indicate the practical values of controllably separable MN patches for versatile drug delivery and clinical applications.
研究领域:
生物医学工程
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药学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
PHYTOMEDICINE
·
2019
·
IF 4.18
Wen-Zhen Dang,Hui Li,Bing Jiang,Kutty Selva Nandakumar,Kai-Fei Liu,Li-Xin Liu,Xiao-Chen Yu,Hui-Jing Tan,Chun Zhou
Background Anti-malarial drug artesunate (ART), a semi-synthetic derivative of artemisnin, has immunosuppressive effects on several autoimmune diseases, including Systemic lupus erythematosus (SLE), Rheumatoid arthritis (RA), and Colitis . However, molecular mechanisms of ART, especially on follicular helper T cells (Tfh), central players in SLE pathology, are far from clear. Purpose The object for this work is to investigate the therapeutic effect of ART on lupus-prone MRL/ lpr mice and its regulatory function on Tfh cells . Study design and methods MRL/ lpr mice were used to explore therapeutic effects of ART on lupus-prone MRL/ lpr mice and its regulatory functions on Tfh cells. Then, experiments of renal function were accomplished using the biochemical kits. Effects of ART on histopathology of kidneys, inflammatory factors and autoantibodies were examined using H&E staining, ELISA and real-time PCR. Flow cytometry and western blot analysis were used to examine effects of ART on Tfh differentiation and Jak2–Stat3 signaling pathway . Results Upon oral administration , ART significantly prolonged the survival of MRL/ lpr mice, ameliorated the lupus nephritis symptoms, decreased the levels of anti-dsDNA antibodies deposited in the kidney, and the levels of pathogenic cytokines (IL-6, IFN-γ and IL-21). After ART treatment , T-cell compartment in the spleen of MRL/ lpr mice was restored in terms of reduction in the number of Tfh cells and in the maintenance of the ratio of Tfr to follicular regulatory T cells (Tfh). In addition, ART has significantly inhibited the phosphorylation levels of Jak2 and Stat3 in the MRL/ lpr mice. Conclusion ART showed therapeutic effects on lupus-prone MRL/ lpr mice by inhibiting the differentiation of Tfh cells as well as altering the activation status of Jak2–Stat3 signaling cascade.
研究领域:
免疫学
·
药理学
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自身免疫疾病
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）