INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hua Zhang,Zhao-Yuan Feng,Zhen-Zhou Li,Yi-Sen Wang,Zhe-Xuan Mu,Xiao-Hong Lv,Zhen-Yu Wang,Zi-Luan Fan
In this study, a polysaccharide component was extracted from Pinus pumila using biomimetic-microwave assisted alkali tandem extraction and then purified by DEAE-52 and Sephadex G-200. A new purified polysaccharide (PSP c-a) was determined through in vitro tracking. The structural features were characterized using a HPGP, FTIR, IC and NMR spectroscopy. The analysis showed that there may be →3, 6)-α-Galp (1 → 3, 4)-α-Glcp (1 → 5)-α-Manp (1→ in the backbone of PSP c-a, →3, 4)-α-Glcp (1→ and →3, 4)-α-Glcp (1 → α-Arap (1→ in the branch of PSP c-a. To improve the effects of PSP c-a on osteoblast MC3T3-E1, PSP c-a was biologically and chemically modified by α-glucosidase together with Sr. Finally, a new thermostable complex Sr-PSP c-a-1 was synthesized. The effects of PSP c-a and Sr-PSP c-a-1 on osteoblasts MC3T3-E1 were compared. It was found that both were found to have a positive effect on MC3T3-E1, and Sr-PSP c-a-1 was more effective than PSP c-a. Therefore, Sr-PSP c-a-1 should be a potential candidate for promoting skeletal tissue regeneration.
研究领域:
生物化学
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材料科学
·
医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhanjun Li,Yibin Xu,Yunwei Liu,Mingru Kong,Jiarong Wang,Yanxia Li,Yuhong Zhao
To efficiently harness resources from Pinus koraiensis seed scales, a type of forestry waste, rigorous studies on the extraction, purification , stability, and free radical scavenging capacity of the proanthocyanidins derived from these seed scales were conducted. Kinetic models showed that under ultrasonic conditions, the proanthocyanidins content reached 2.66 mg/g within 0.5 h. The optimal storage parameters include darkness, 4 °C, and pH 4. The degrees of polymerization of the mixture and the high- and low-polymer components were 4.89, 7.42 and 3.07, respectively, with the low-polymer component exhibiting the highest radical scavenging activity. Through HPLC–QE-MS/MS, 1 H NMR, and FT-IR analyses, we identified proanthocyanidin B1, proanthocyanidin B2, (−)-epicatechin, and polymeric trimer esters. The Pinus koraiensis proanthocyanidins exhibited a high molecular weight, a complex internal molecular structure, and commendable stability, with crystallization requiring elevated temperatures. Therefore, the proanthocyanidins from Pinus koraiensis seed scales have emerged as highly promising novel natural antioxidants .
研究领域:
林学
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天然产物化学
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生物化学
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DOI
PubMed
高影响因子
Journal of Applied Research on Medicinal and Aromatic Plants
·
2022
·
IF 3.945
Zhanjun Li,Yibin Xu,Yunwei Liu,Ke Ma,Kunlun Wang,Fengjian Yang
Tea saponin was used for UAE of oil from Pinus koraiensis seed scales for the first time, and the extraction kinetic model was fitted and compared with that of traditional SE. Considering the relationship among the oil yield, proanthocyanidins content and solvent properties, ethanol was selected as the optimum extraction solvent. PBD and BBD were used to determine the optimal conditions: 63% aqueous ethanol solution, liquid-to-material ratio was 11:1 mL/g, ultrasonic power was 100 W, ultrasonic duration was 15 min, temperature was 45 °C, and the dose of tea saponin was 0.3%. Under the optimal conditions, the oil yield and proanthocyanidins content from P. koraiensis seed scales were 22.73 ± 0.42% and 3.04 mg/g ± 0.08%, respectively. The actual operating value was consistent with the theoretically predicted value. Comparison of the extraction kinetics model, oil components, physical and chemical properties, antioxidants, microstructure of oil residue, energy consumption and ecological emission characteristics of UAE and SE shows that UAE is a more advantageous technique.
研究领域:
植物化学
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食品科学与工程
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Zhanjun Li,Yunwei Liu,Mingru Kong,Yibin Xu,Kunlun Wang,Ke Ma,Qi Shen,Qingfen Zhang
To increase the extraction efficiency of quercetin from Pinus koraiensis seed scales (PKSS), an innovative chitosan-Cu-Fe (CS-Cu-Fe) ionic liquid system for efficient ultrasonic-assisted extraction (UAE) of PKSS quercetin (PKSSQ) was designed. A first-order kinetic model was applied to analyse the extraction performance, and key factors influencing the process were systematically optimized. The optimal conditions were as follows: 2.00 g sample, 0.016 mol [BMIM]Br, 0.6 %, 100 W, 53°C, 12:1 mL/g, and 14 min. Under these conditions, the actual PKSSQ yield was 2.92 mg/g ± 0.02 mg/g, which closely aligned with the theoretical yield of 2.96 mg/g. The extraction kinetics were compared across different methods, and the stability of the CS-Cu-Fe system was evaluated through recovery experiments. The CS-Cu-Fe was characterized using Fourier transforms infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), X-ray diffraction (XRD), hysteresis loop (HL) measurement, electrical conductance (EC) measurement and scanning electron microscopy (SEM) of the extracted residues. CS-Cu-Fe demonstrated exceptional thermal stability, magnetic properties, and catalytic activity, significantly enhancing the PKSSQ extraction efficiency. FT-IR analysis revealed minimal spectral changes in CS-Cu-Fe, with notable EC differences. Owing to its stable core functional groups, CS-Cu-Fe maintained excellent thermal stability, crystal structure, soft magnetism, and catalytic efficiency. This study highlights the efficiency of the CS-Cu-Fe ionic liquid system in UAE of PKSSQ and the role of its stable core functional groups in improving the extraction and catalytic performance. This innovative approach provides a robust theoretical foundation for extracting and developing natural products from PKSS, with promising future applications.
研究领域:
植物化学
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天然产物开发
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药用植物研究
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产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 46
·期 1
·e16145
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IF 2.19
Jiayi An,Nadya Mara Adelina,Ligang Zhang,Yuhong Zhao
In this study, pine nut kernels from two grafted species: Pinus koraiensis (PK) and Pinus sylvestris var . (PKS) were roasted (130, 150, 180℃ for 10, 20, 30 min) to investigate the effect of roasting pre-treatment on chemical components, antioxidant activity, and oxidative stability of oils prepared from kernels. Increased temperature or time was found to cause a significant increase in oil yield, color, total phenolic content (TPC), carotenoid, and browning index (BI) of oils compared to unroasted samples. Roasting could also elevate the antioxidant activity and oxidative stability of pine nut oil. The levels of tocopherol decreased significantly at high temperatures (150℃, 180℃), while the fatty acid compositions were almost independent of roasting conditions. The FTIR spectra of oils from different roasted pine nuts showed minor variation in peak intensities. Therefore, roasting is a great option and critical step to improve the quality of pine nut oil. Novelty impact statement The oil yield, color, total phenolic content (TPC), carotenoid, and browning index (BI) of pine nut oil from two grafted species were influenced by different roasting conditions. Roasting could improve the antioxidant activity and oxidative stability of pine nut oil. Roasting (180℃ for 10 min) is a great option to improve the quality of pine nut oil.
研究领域:
食品科学
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化学
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农业
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 21
·3851
·
IF 3.06
Yandong Zhang,Chao Xin,Junqiang Qiu,Zhenyu Wang
Pinecone is a traditional folk herb, which has been used in China for many years. In this paper, the essential oil fromPinus koraiensispinecones (PEO) was obtained by hydrodistillation and 41 compounds were identified by gas chromatography–mass spectrometry (GC-MS), mainly including α-Pinene (40.91%), Limonene (24.82%), and β-Pinene (7.04%). The purpose of this study was to investigate the anti-tumor activity of PEO on MGC-803 cells and its mechanism. Anti-tumor experiments in vitro showed PEO could significantly inhibit the proliferation and migration of MGC-803 cells, and it also could arrest the cell cycle in the G2/M phase, decrease the mitochondrial membrane potential, and induce apoptosis. Finally, the effects of PEO on genes expression on MGC-803 cells were analyzed by RNA sequencing, and results showed that after treatment with PEO, 100 genes were up-regulated, and 57 genes were down-regulated. According to the KEGG pathway and GSEA, FAT4, STK3, LATS2, YAP1, and AJUBA were down-regulated, which were related to HIPPO signaling pathway. Real-time PCR and western blot further confirmed the results of RNA sequencing. These results indicated that PEO may exert anti-tumor activity via the HIPPO/YAP signaling pathway. The anti-tumor mechanism of this oil can be further studied, which is important for the development of anti-tumor drugs.Keywords:pinecone;essential oil;gastric cancer;RNA sequencing;HIPPO/YAP signaling pathway
研究领域:
药理学
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肿瘤学
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分子生物学
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植物科学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Zhanjun Li,Yibin Xu,Yunwei Liu,Mingru Kong,Ke Ma,Jiarong Wang,Honggang Wang,Yuhong Zhao
In this work, in situ extraction of essential oil from Pinus koraiensis seed scales using tea saponin-induced ultrasonic pretreatment-microwave-assisted hydrodistillation (UP-MAHD) was studied. By fitting the extraction kinetic curves with different factors, a first-order kinetic model was determined to be more suitable. The key factors were screened and optimized via Plackett Burman Design (PBD) and BOX-Behnken Design (BBD). Under the optimization parameters of a liquid material ratio of 14 mL/g, power of 100 W, time of 15 min, temperature of 50 °C, and tea saponin dose of 0.4% for the ultrasonication and then microwave at 458 W for 18 min, the actual yield was 8.02 μL/g± 0.35% and was highly consistent with the theoretical yield of 8.21 μL/g. UP-MAHD-treated Pinus koraiensis seed scales essential oil (PKSSEO) had good inhibition functional activity of α-amylase . In addition, the chemical composition of the PKSSEO were determined using Gas chromatography mass spectrometry (GC MS) and Fourier transform infrared spectroscopy (FT IR), thermal gravimetry (TG), and differential scanning calorimetry (DSC), and its spatial structure characterization (SSC) obtained via UP-MAHD treatment was enhanced. In summary, this extraction method efficiently extracted PKSSEO with a lower effect on its activity and composition and could have solid future development prospects. Our study provided effective theoretical data support for the reuse and development of abandoned Pinus koraiensis seed scales resources.
研究领域:
植物化学
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食品科学与工程
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中药资源学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Hang Qu,Xin Gao,Cuilin Cheng,Haitian Zhao,Zhenyu Wang,Juanjuan Yi
Previous studies have shown that Pinus koraiensis pine nut polysaccharide PNP80b-2 exerts widely protective effects against liver injury induced by chemical pollutants, alcohol and drugs. By comparison, PNP80b-2 exhibits the strongest hepatoprotection against alcohol-induced liver injury (AILI). Thus, the purpose of this study is to investigate the hepatoprotection mechanisms of PNP80b-2 against AILI in vivo . The results indicated that PNP80b-2 alleviated oxidative stress induced by alcohol through enhancing antioxidant capacity of hepatocytes via NRF2/HO-1 pathway. PNP80b-2 also effectively suppressed the secretion of pro-inflammatory cytokines including TNF-α, IL-1β and IL-6, exhibiting anti-inflammatory effects via NF-κB signaling pathway in AILI. In addition, PNP80b-2 protected mice from severe DNA damage induced by alcohol through regulating the expression of Hipk2 , P53 , Hp1γ and Wip1 . Taken together all the results, PNP80b-2 exerts hepatoprotective activity against AILI in vivo through enhancing antioxidant capacity, suppressing inflammation response and promoting DNA damage repair in livers. Furthermore, the structural features of PNP80b-2 were also characterized. PNP80b-2, with molecular weight of 23.0 kDa, was found to be composed of 1,2-linked Gal f , 1,2-linked Rha p , 1,4-linked Xyl p , 1,6-linked Glc p , 1,4-linked Glc p A, 1,2,6-linked Gal p , 1,4,6-linked Glc p , 1,2,3,4-linked Ara p , 1-linked Gal p and Leu- and Ile-linked O-glycopeptide bonds, based on the GC–MS and NMR results.
研究领域:
药理学
·
生物化学
·
分子生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Hang Qu,Xin Gao,Hai-Tian Zhao,Zhen-Yu Wang,Juan-Juan Yi
A new purified polysaccharide (PNP40c-1) with a molecular weight of 2.06 × 10 5 Da was obtained from pine nuts ( Pinus koraiensis Sieb. et Zucc. ). Structural analysis indicated that PNP40c-1 is a homogeneous heteropolysaccharide composed of arabinose, rhamnose and glucose in a molar ratio of 2.98:1.00:0.52. The major backbone consisted of →3,4)- α - l -Ara p (1→, →4)- α - l -Ara p 3Me(1→, →3)- α - l -Rha p (1→ and →6)- β - d -Glc p (1→, and the side chain is β - d -Glc p -(1→ linked at C4-position of →3,4)- α - l -Ara p (1→. In addition, the hepatoprotective effect of PNP40c-1 was investigated by human hepatocyte cell line L02 treated with carbon tetrachloride (CCl 4 ). Results suggested that PNP40c-1 could protect L02 cells from CCl 4 -induced damage by enhancing the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), increasing the level of non-enzymatic antioxidant glutathione (GSH), suppressing lipid perioxidation and further reducing the leakage of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Hence, PNP40c-1 could be a promising functional food to serve as hepatoprotective agent.
研究领域:
生物化学
·
药理学
·
营养学
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产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2019
·
IF 6.395
Kexin Hou,Meili Bao,Lu Wang,Hua Zhang,Lei Yang,Haitian Zhao,Zhenyu Wang
In this study, in order to simultaneously extract procyanidins and essential oil from pinecones of Pinus koraiensis , aqueous enzymatic pretreatment ionic liquid–lithium salt based microwave–assisted extraction (AEILLME) with a modified hydrodistillation concatenated Soxhlet extraction apparatus was proposed. 1-Butyl-3-methylimidazolium bromide solution was selected as extraction solvent , while LiCl and mixture of cellulase and pectinase (1:1, w/w) as additives by single factor experiment. Other parameters that affect the yield of target components were investigated and optimized by Plackett–Burman design and Box–Behnken design. The obtained optimal conditions were: microwave irradiation power 581.49 W, microwave irradiation time 15.95 min and liquid–solid ratio 11.91 mL/g. Under the optimized conditions, the predicted yields of oligomeric procyanidins, polymeric procyanidins and essential oil were 13.02, 32.19 and 7.76 mg/g, respectively. Analysis of chemical constituents in the essential oil was performed on gas chromatography–mass spectrometry and there was no significant difference on the components of essential oil obtained by proposed method and traditional methods. Ionic liquids were successfully recycled and reused, which could lower the process cost and being beneficial to industrial production. This study provides an approach with good prospects for the application in extracting active components from natural plant material.
研究领域:
化学工程
·
分析化学
·
植物化学
·
天然产物
·
提取技术
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）