NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 15
·12954-12959
·
IF 3.201
Tao Gong,Rong Li,Yangyang Yuan,Baofeng Yu,Hong Zhao,Zhizhen Liu,Rui Guo,Dan Su,Wenting Liang,Chuan Dong
A turn-on fluorescent probe (E)-4-(2-(1H-benzo[d]imidazol-2-yl)vinyl)-N,N-diphenylaniline (BVD) was facilely synthesized via the ethylene bridging of triphenylamine and benzimidazole. The probe exhibited strong emission enhancement in the far-red fluorescence region at 605 nm with a pKa value of 4.14 and linear response to the acidity range of 4.52–3.37. BVD displayed a large Stokes shift of 105 nm under acidic conditions, high sensitivity, quick response, excellent reversibility and good photostability, which are favorable for monitoring the pH variation in real time. More importantly, BVD had excellent cell membrane permeability and was applied successfully to visualize intracellular pH fluctuations in living cells without the influence of autofluorescence.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 10
·3781-3787
·
IF 4.58
Zhang Pu,Wang Yi,Chen Ling,Yin Yibing
Gold-silver nanoclusters (Au-AgNCs) were synthesized by simultaneous chemical reduction of Au(III) and Ag(I) ions in one pot, using bovine serum albumin as both a template and a reductant. The Au-AgNCs have an average size of 2.4 nm and display strong red fluorescence (with an emission peak at 610 nm on excitation at 360 nm). The fluorescence quantum yield can reach 18.6%. Fluorescence is strongly quenched by hypochlorite, while other common anions have minor (or no) effects on fluorescence. Based on these findings, a fluorometric method was developed for the determination of hypochlorite. The method has a linear response in the 0.7 to 15 μM concentration range, with a limit of detection as low as 80 nM. It was successfully applied to the determination of hypochlorite in (spiked) tap water.
Gold-silver nanoclusters with strong red fluorescence were synthesized by simultaneous chemical reduction of Au(III) and Ag(I) ions in one pot, and a sensitive and selective method for the detection of hypochlorite was developed based on the quenching of the fluorescence of the nanoclusters.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICAL MICRODEVICES
·
2021
·Vol. 23
·期 4
·1-7
·
IF 2.838
Yao Yin,Fan Yiqiang
This study proposed a rapid and low-cost fabrication method for open-channel hydrogel-based microfluidic devices using CO 2 laser ablation. The agarose hydrogel substrate was prepared with agarose gelation in DI water upon microwave heating, then a commercial CO 2 laser system was used for the direct laser ablation of microchannels on the surface of agarose hydrogel substrate, the hydrophilic nature of the microchannels fabricated on hydrogel substrate enables the self-driven of the liquid inside the microchannels with capillary force. The profiles of the laser ablated microchannels on agarose hydrogel substrate with various laser power and scan speed were studied in detail. Due to the loss of water when exposed to the atmosphere, significant deformation of the fabricated microchannels was observed, and the profile change was recorded for 48 h for comparison. An easy-to-access storage method of hydrogel-based microfluidic device in DI water was also proposed in this study. Unlike compact silicon or polymer-based microfluidic devices, the hydrogel is formed by cross-linked polymer chains filled with water, for a better understanding of the diffusion of small molecules into the bulk hydrogel material during fluid propagation inside the microchannel, the Nile red fluorescent was added into the liquid and the diffusion across the hydrogel-based microchannels with time was measured and discussed in this study. Several open-channel agarose hydrogel-based microfluidic devices were fabricated in this study for the demonstration of the proposed fabrication method. The CO 2 laser ablation approach for agarose hydrogel-based microfluidic devices has the advantages of rapid processing time, low-cost, highly biocompatible, and self-driven without pumps and could have wide application potentials in biological and medical fields.
研究领域:
微流控
·
生物医学工程
·
材料科学
·
激光技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Yanzi Xu,Haoke Zhang,Ning Zhang,Xiaochi Wang,Dongfeng Dang,Xunan Jing,Duo Xi,Ying Hao,Ben Zhong Tang,Lingjie Meng
To achieve super-resolution imaging in biological research using stimulated emission depletion (STED) nanoscopy, organic luminescent materials and their corresponding fluorescent nanoparticles with high brightness and photostability are of great significance. Herein, donor-acceptor-typed DBTBT-4C8 bearing flexible alkyl chains was developed, not only to afford deep-red emission from 600 to 800 nm but also to obtain high fluorescent brightness with the absolute photoluminescence quantum yields of 25%. After that, well-defined and monodispersed spherical nanoparticles using DBTBT-4C8 with bright emission, excellent biocompatibility, and photostability, which can easily mix with amphipathic block polymers, were then produced for super-resolution in vitro and in vivo imaging using STED nanoscopy. The observations showed that in contrast to confocal microscopy with a full width at half-maximum (FWHM) value of ≈400 nm, superior resolution with a significantly improved FWHM value of only 100 nm was achieved in biomedical cell imaging, which was also used to reconstruct three-dimensional images of stained HeLa cells at an ultrahigh resolution. More importantly, by using the prepared fluorescent organic nanoparticles (FONPs) in STED nanoscopy, in vivo imaging in glass catfish with largely enhanced resolution was also successfully achieved, demonstrating that these developed deep-red FONPs here are highly suitable for super-resolution in vitro and in vivo imaging using STED nanoscopy.
研究领域:
生物研究
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材料科学
·
纳米技术
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）