FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Chao Qi,Yueying Jin,Shengyu Cheng,Lei Di,Xin Wang,Minghui Zhang,Lingli Zhang,Xi-Ling Li,Yu Han,Qingkun Ma,Jun Zhe Min
α-dicarbonyl compounds (α-DCs) serve as potential biomarkers for oxidative stress-related diseases but are difficult to detect. To study the metabolism of carbonyl compounds , we developed a new mass spectrometry probe, 3-benzyl-2-oxo-4λ 3 -thiazolidine-4-carbohydrazide (BOTC), containing hydrazyl groups for the targeted detection of carbonyl functional groups. In a novel approach, we used BOTC pre-column derivatization with ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) to simultaneously detect four kinds of α-DCs in red wine as well as in urine after drinking. The α-DCs were completely separated (R 2 ≥ 0.9995), detection was sensitive (detection limit was 12.5–50 fmol), consistent (intraday and interday precision was 0.1–5.7 %), and efficient (average recoveries were 103.3–110.2 %). The method was applied to the analysis of α-DCs in different wines and the dynamic monitoring of transit and excretion in vivo after drinking. Our novel method provides a new strategy for the detection of α-dicarbonyl compounds in red wine and dicarbonyl compounds produced in oxidative stress-related diseases.
研究领域:
分析化学
·
医学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2022
·
IF 1.193
Yu-Ye GAO,Xie-An YU,Bing WANG,Li-Fei GU,Yuan-Yuan GE,Guang-Lin ZHU,Kun-Hui SUN,Yi LU,Tie-Jie WANG,Kai-Shun BI
Red wine was famous for the potential effects on coronary heart disease (CHD), but the pharmacokinetic study of the main active compounds was unclear. In this research, a simple, rapid and sensitive ultra-fast liquid chromatography coupled with triple quadrupole mass spectrometry (UFLC-MS/MS) method was established for simultaneous determination of six phenolic acids and six flavonoids after oral administration of red wine in rat plasma. Moreover, the pharmacokinetic differences of the six active compounds in control and CHD model rats after oral administration of red wine were investigated. Rosmarinic acid and puerarin were selected as internal standards. Extraction of plasma sample was by protein precipitation using acetonitrile . An HSS T3 column (1.8 µm, 2.1 mm × 100 mm) was used to separation at a flow rate of 0.3 mL/min with mobile phase consisting of 0.1% formic acid aqueous solution and 0.1% formic acid acetonitrile. The developed method was successfully applied to comparative pharmacokinetic study between control and CHD model rats. The results showed that some pharmacokinetic parameters were significantly different among the control and CHD model rats. Accordingly, it was expected that the results of this study could provide a scientific basis for further exploring red wine for the prevention and treatment of CHD.
研究领域:
药理学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Zhengbo Guo,Hanyue Dong,Junxia Lin,Yongyuerun Hu,Dabing Ren,Lunzhao Yi,Siyu Li
In this research, accelerated aroma release experiments and malvidin-3- O -glucoside copigmentation experiments in model red wine solutions were designed to investigate the abilities and molecular mechanisms of mannoproteins in modulating olfactory/chromatic properties of red wines. Results indicate that under orthonasal condition, mannoprotein MP2 was promising aroma modulator due to its predictable behaviors in expelling and retaining the aroma compounds during different periods. Low field nuclear magnetic resonance and molecular dynamic simulation proved that the modulation ability of MP2 should be explained by its transitionary interacting preferences with water/aroma compound molecules. Retronasal results show that the release of aroma compounds and olfactory perceptions were irregular and difficult to predict, probably due to the complexity of the retronasal condition. All mannoproteins protected malvidin-3- O -glucoside and quercetin via the formation of binary/ternary complexes, and quercetin was found prior to be protected than malvidin-3- O -glucoside. Principal mannoprotein A0A6C1DV26 might be the critical malvidin-3- O -glucoside protector. With the presence of quercetin, principal mannoproteins B3LQU1/B5VL26 in mannoprotein MP1 might exhibit intramolecular and/or intermolecular mechanisms that strengthened the hyperchromic effect, thus enhanced the copigmentation.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2019
·
IF 3.579
Hao Suo,Ruochen Tian,Jing Li,Shuting Zhang,Yan Cui,Lingxi Li,Baoshan Sun
High-molecular-mass polymeric polyphenols (HPPCs) is the major group of polyphenols in red wine. However, owing to the diversity and structural complexity, its compositional characterization remains a difficulty. In this study, purified high-molecular-mass polymeric polyphenols (PHPPCs) was isolated by solid phase extraction combined with semi-preparative HPLC from red wine. Different chemical degradation methods (NaOH, HCl and benzyl mercaptan degradation) were used to decompose the PHPPCs into low molecular weight fragments. The chemical structures of the degradation fragments were verified by HPLC/UPLC−DAD and HPLC-FT-ICR-MS. The results showed that three proanthocyanidins, five proanthocyanidins benzylthioether, quercetin and fifteen anthocyanins were detected by degradation in the presence of benzyl mercaptan. Nine bound phenolic acids were found by NaOH degradation and twelve bound amino acids were proved by HCl. Among these different fragments detected,delphinidin-3-O-glucoside pyruvic adduct, four acylated anthocyanins, four hydroxyphenyl pyranoanthocyanins, quercetin, protocatechuic acid, vanillic acid, and cinnamic acid were first found in the PHPPCs. For the first time amino acids as constitutive units of polymeric polyphenols in red wines were verified. Based on the degradation fragments, the possible compositional characterization of HPPCs in red wine was postulated.
研究领域:
食品科学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2016
·
IF 2.687
Zerong Long,Weiwei Xu,Yi Lu,Hongdeng Qiu
1 A new and facile rhodamine B (RhB)-imprinted polymer nanoshell coating for SiO 2 nanoparticles was readily prepared by a combination of silica gel modification and molecular surface imprinting. The RhB-imprinted polymers (RhB-MIPs) were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and UV–vis spectroscopy; the binding properties and selectivity of these MIPs were investigated in detail. The uniformly imprinted nanoparticles displayed a rather thin shell thickness (23 nm) with highly effective recognition sites, showing homogenous distribution and monolayer adsorption. The maximum MIP adsorption capacity ( Q m ) was as high as 45.2 mg g −1 , with an adsorption equilibrium time of about 15 min at ambient temperature. Dynamic rebinding experiments showed that chemical adsorption is crucial for RhB binding to RhB-MIPs. The adsorption isotherm for RhB-MIPs binding could also be described by the Langmuir equation at different temperatures and pH values. Increasing temperature led to an enhanced Q m , a decreased dissociation constant ( K' d ), and a more negative free energy (Δ G ), indicating that adsorption is favored at higher temperatures. Moreover, the adsorption capacity of RhB was remarkably affected by pH. At pH > 7, the adsorption of RhB was driven by hydrogen bonding interactions, while at pH < 7 electrostatic forces were dominant. Additionally, the MIPs also showed specific recognition of RhB from the standard mixture solution containing five structurally analogs. This method was also successfully employed to determine RhB content in red wine and beverages using three levels of spiking, with recoveries in the range of 91.6–93.1% and relative standard deviations lower than 4.1%.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 6
·2496-2511
·
IF 3.9
Wenqing Hu,Fan Gong,Qingfeng Dong,Yongfeng Kang,Yan Zhou,Boqiang Liu,Li Li
Red grapes ( Vitis vinifera L.) have a high sugar content, thin skins, and relatively short shelf-life after harvesting. We developed polylactic acid/polybutylene succinate film, prepared by extrusion of polylactic acid and polybutylene succinate, that significantly prolonged the shelf-life of red grapes from 8 to 12 days by delaying the loss of weight, loss of hardness, and reduction in soluble solid content after harvesting. Further mechanistic study showed that this modified atmosphere film delayed the senescence of harvested red grape, the phenomenon that was highly related to the lower active oxygen species production and higher antioxidant enzyme activity compared to the non-packaged grape. The proposed continuous and dynamic microenvironment regulation system is a promising method to study the mechanisms of respiratory metabolism in fruit and extends food shelf-life while reducing food waste. Practical Application In this study, we designed a means to microenvironmental regulatory packaging that directly creates a continuous, dynamic, and monitorable microenvironment. To ensure that the fruit inside the package underwent coordinated aerobic and anaerobic respiration, we melt-extruded polylactic acid (PLA) and polybutylene succinate (PBS) to form a homogeneous biodegradable film. We demonstrated that the best preservation results were achieved with a film comprising an 80/20 PLA-to-PBS ratio. This film can prolong the shelf-life of fruits by regulating respiratory metabolism.
研究领域:
食品科学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Keyuan Pu,Yue Wang,Huiwen Wei,Jun Hu,Jiamin Qiu,Siyu Chen,Qian Liu,Yan Lin,Kwan-Ming Ng
BACKGROUND The commercial value of red wine is strongly linked to its geographical origin. Given the large global market, there is great demand for high-throughput screening methods to authenticate the geographical source of red wine. However, only limited techniques have been established up to now. RESULTS Herein, a sensitive and robust method, namely probe electrospray ionization mass spectrometry (μ-PESI-MS), was established to achieve rapid analysis at approximately 1.2 min per sample without any pretreatment. A scotch near the needle tip provides a fixed micro-volume for each analysis to achieve satisfactory ion signal reproducibility (RSD < 26.7%). In combination with a machine learning algorithm, 16 characteristic ions were discovered from thousands of detected ions and were utilized for differentiating red wine origin. Among them, the relative abundances of two characteristic metabolites (trigonelline and proline) correlated with geographical conditions (sun exposure and water stress) were identified, providing the rationale for differentiation of the geographical origin. CONCLUSION The proposed μ-PESI-MS-based method demonstrates a promising high-throughput determination capability in red wine traceability.
研究领域:
食品科学
·
分析化学
·
机器学习
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Shiyao Wang,Xinyi Jia,Shuangli Xiong,Yisheng Chen
This study investigated the effects of low-power, low-temperature, and short-duration ultrasonic treatment on the quality of sweet red wine (SRW) to optimize flavor aging. Experiments were conducted by varying temperature (10–30 °C), duration (30–180 min), and power (100–300 W), with subsequent analysis of total sugar, acidity, tannins, esters, phenolic content, and antioxidant activity. Results demonstrated that low-power and low-temperature ultrasonic treatment effectively modulated the sugar-acid ratio in SRW. Tannin content exhibited minimal temperature dependence but was significantly influenced by ultrasonic power and duration, with duration exerting the strongest effect. Total ester content decreased with prolonged ultrasound exposure, though this reduction was mitigated under low-power conditions. Phenolic content declined as ultrasonic intensity increased. Sensory evaluation identified optimal aging parameters at 20 °C, 90 min, and 200 W. These results confirm that low-power, short-term ultrasound effectively ages sweet red wine while improving quality. This method offers a practical alternative to traditional aging, with potential benefits for wine production efficiency and quality control.
研究领域:
食品科学
·
酿酒工程
·
声化学
·
食品加工技术
·
分子化学
·
感官科学
·
抗氧化研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2017
·
IF 1.392
Liqiang Liu,Liguang Xu,Steven Suryoprabowo,Shanshan Song,Hua Kuang
Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2018
·Vol. 65
·期 9
·1090-1097
·
IF 0.862
Chunyan Li,Chaozhan Wang,Yinmao Wei
Boronate-affinity adsorbents have been regarded as favorable extraction adsorbents for the pretreatment of cis -diol-containing biomolecules owning to their specific selectivity, but most of them have low adsorption capacity and a tedious synthesis methods. In this study, a new boronate-affinity material (PGMA@FPBA) with high adsorption capacity was synthesized via a “one-pot” method based on a low-cost commercial support. The PGMA@FPBA was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM), and nitrogen adsorption/desorption measurements. The as-prepared adsorbent showed good selectivity, high adsorption capacity (448 μmol/g for catechol), and fast adsorption equilibration (1 min) for cis -diol-containing biomolecules. Subsequently, as an example for application, the obtained PGMA@FPBA was used as a dispersive solid-phase extraction (d-SPE) adsorbent for enrichment of quercetin in red wine. The results indicated that the facile-prepared boronate-affinity adsorbent has great potential application for separation and enrichment of cis -diol-containing biomolecules in complex samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 8
·1139-1144
·
IF 0
Gu Mengqiao,Liu Jiali,Li Delei,Wang Min,Chi Kuanneng,Zhang Xi,Deng Yan,Ma Yuchan,Hu Rong,Yang Yunhui
In this paper, a signal-off aptasensor was constructed based on the specificity of OTA and OTA aptamer (DNA2), it can detect the small molecule ochratoxin A (OTA). DNA1 chain with mercapto modified at 3′-end was firstly immobilized on gold electrode, which cross-fertilize with
DNA 2 to form double strands. 30 thymine T modified at the end of DNA2 were used as templates to synthesize Cu NCs which is peroxidase mimics and can catalyze hydrogen peroxide. Presence of OTA, it specifically binds to DNA2, which made DNA2 dissociated, and so did Cu NCs. The more OTA in
solution, the more the aptamers and Cu NCs dissociated from the gold electrode, the less copper nanoclusters remained on the electrode, and the smaller the current response value of the electrode measured by chronoamperometry. Thus, the quantitative determination of OTA can be achieved. This
aptasensor provides a simple and fast technique for OTA detection in red wine samples.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
生物传感器
✓ 已验证
DOI
FOOD CHEMISTRY
·
2019
·
IF 5.399
Shao Hua Lu,Sha Sha Li,Bo Yin,Jia Ying Mi,Hong Lin Zhai
Diverse interferences often affect the determination of pesticide residues in various kinds of food, and complex pretreatments are necessary. An effective approach for the simultaneous quantitative analysis of multi-target components in different substrates was proposed, and applied to the determination of ternary pesticides in cherry tomatoes and red grape samples. By means of HPLC-DAD, the spectra were obtained under isocratic elution. Utilizing the Tchebichef image moments, unified quantitative models were established for the three target components in the samples with two different substrates, respectively. The correlation coefficients of leave-one-out cross-validation ( R loo - c v 2 ) of the obtained models were more than 0.9975. The predictive correlation coefficients ( R p 2 ) were more than 0.9831. Compared with the N-PLS and MCR-ALS methods, the proposed approach is not only more accurate and reliable, but also can extract essential information and expected to be a potential tool to rapidly quantify multi-component in different substrates without complex pretreatments.
研究领域:
分析化学
·
食品科学
·
化学计量学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）