Journal of Hazardous Materials Advances
·
2025
·
IF 7.7
Peigen Chen,Lina Chen,Xing Yang,Tingting Li,Cong Fang
The pervasive contamination by Di(2-ethylhexyl) phthalate (DEHP) and nanoplastics (NPs) poses a significant threat to reproductive health, yet the combined effects of these pollutants on endometrial receptivity remain poorly understood. Our study investigates the impact of chronic DEHP and NPs co-exposure on endometrial ciliary function in mice and explores the potential protective role of dietary inulin. Utilizing a multifaceted approach—including transcriptomic analysis, immunohistochemistry, scanning electron microscopy, and immunofluorescence—we reveal that DEHP and NPs synergistically impair endometrial cilia, crucial for embryo implantation. Remarkably, inulin supplementation restores ciliary structure and function, mitigating the detrimental effects of these pollutants. Our findings suggest that dietary interventions could offer a practical strategy to counteract the reproductive toxicity of environmental contaminants, providing new insights into maintaining reproductive health in polluted environments. This research underscores the urgency of addressing environmental pollutants and highlights the potential of inulin as a protective agent against reproductive toxicity.
研究领域:
生殖生物学
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环境医学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
CELL
·
2022
·
IF 66.85
Shu-Hsien Sheu,Srigokul Upadhyayula,Vincent Dupuy,Song Pang,Fei Deng,Jinxia Wan,Deepika Walpita,H. Amalia Pasolli,Justin Houser,Silvia Sanchez-Martinez,Sebastian E. Brauchi,Sambashiva Banala,Melanie Freeman,C. Shan Xu,Tom Kirchhausen,Harald F. Hess,Luke Lavis,Yulong Li,Séverine Chaumont-Dubel,David E. Clapham
Summary Chemical synapses between axons and dendrites mediate neuronal intercellular communication. Here, we describe a synapse between axons and primary cilia: the axo-ciliary synapse. Using enhanced focused ion beam-scanning electron microscopy on samples with optimally preserved ultrastructure, we discovered synapses between brainstem serotonergic axons and the primary cilia of hippocampal CA1 pyramidal neurons. Functionally, these cilia are enriched in a ciliary-restricted serotonin receptor, the 5-hydroxytryptamine receptor 6 (5-HTR6). Using a cilia-targeted serotonin sensor, we show that opto- and chemogenetic stimulation of serotonergic axons releases serotonin onto cilia. Ciliary 5-HTR6 stimulation activates a non-canonical G αq/11 -RhoA pathway, which modulates nuclear actin and increases histone acetylation and chromatin accessibility. Ablation of this pathway reduces chromatin accessibility in CA1 pyramidal neurons. As a signaling apparatus with proximity to the nucleus, axo-ciliary synapses short circuit neurotransmission to alter the postsynaptic neuron’s epigenetic state.
研究领域:
神经科学
·
细胞生物学
·
分子生物学
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DOI
PubMed
高影响因子
CELLULAR SIGNALLING
·
2025
·
IF 3.7
Yuyan Sun,Ziyu Luo,Haiqi Zhou,Sha Wu,Hongwei Shen,Yuanyuan Fu,Thai Namanh Ngo,Wen Wang,Yuanrong Wang,Ying Kong
Chondrocyte mechanotransduction dysfunction is a key feature of osteoarthritis (OA). Previous studies have demonstrated that primary cilia play a crucial role in chondrocyte mechanosensation and signal transduction. Transient Receptor Potential Vanilloid 4(TRPV4), which is highly expressed on primary cilia, is a critical mechanotransduction protein and one of the most important Ca 2+ channels. However, the interplay between the mechanism of primary cilia-mediated signaling and that of TRPV4 channel regulation remains poorly understood. Here, we demonstrate that OA chondrocytes and Intraflagellar Transport Protein 88(Ift88) conditional knockout (cKO) mice exhibit reduced ciliary incidence. This results in cartilage degeneration. TRPV4 expression inversely correlated with ciliary integrity, increasing as cilia were lost. In cilia-intact healthy chondrocytes, TRPV4 antagonism inhibited Ca 2+ -dependent NF-κB activation while promoting type II collagen(COL2) synthesis. These effects were abolished by Interleukin-1 beta(IL-1β)-induced ciliary defect or IFT88 deficiency. Chromatin immunoprecipitation(ChIP) analysis revealed disrupted p65 binding to the COL2 promoter upon the loss of ciliary integrity. Crucially, Ift88 cKO chondrocytes showed unresponsive of the TRPV4 channel, as confirmed by Ca 2+ flux and changes in the expression levels of NF-κB and COL2. Our findings position primary cilia as indispensable regulators of TRPV4-mediated NF-κB - COL2 signaling, offering mechanistic insights into OA pathogenesis and identifying IFT88 as a therapeutic checkpoint.
研究领域:
分子生物学
·
细胞生物学
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骨关节炎病理学
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信号转导
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转化医学
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软骨生物学
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机械生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2020
·
IF 3.421
Menglong Sun,Qian Wang,Baiying Dai,Weihua Sun,Yaru Ni,Chunhua Lu,Jiahui Kou
A simple combination of thermal etching and liquid-phase ultrasonic stripping was deployed to prepare high-performance graphite carbon nitride (g-C3N4) nanosheets. Also, graphene oxide with plentiful active groups was used to combine g-C3N4 with a magnetically actuated artificial cilia array (MAACA) to generate a composite cilia array, which can ensure the facile recovery of g-C3N4. This strategy addresses the limitations of an adjustable band gap for easy response to visible light and circumvents the encumbered limitation of recyclability, thus addressing a chronic environmental problem by curbing waste. The underlying functional principle of the array used was analyzed and studied in greater detail. The photocatalytic hydrogen production efficiency of MAACA with 0.4 mg of g-C3N4 at a magnetic field driving frequency of 12.50 Hz was found to be ∼75.0% higher than that at a magnetic field driving frequency of 0 Hz.
研究领域:
材料科学
·
环境科学
·
能源科学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Xiao-Qian Chen,Zhi-Yong Guo,Lei Wang,Zheng-Fei Yan,Chang-Xu Jin,Qing-Song Huang,De-Min Kong,De-Ming Rao,Jing Wu
Poly (ethylene terephthalate) (PET) is a widely used type of general plastic that produces a significant amount of waste due to its non-degradable properties. We propose a novel directional-path modification (DPM) strategy, involving positive charge amino acid introduction and binding groove remodeling, and apply it to Thermobifida fusca cutinase to enhance PET degradation. The highest value of PET degradation (90%) was achieved in variant 4Mz (H184S/Q92G/F209I/I213K), exhibiting values almost 30-fold that of the wild-type. We employed molecular docking, molecular dynamics simulations, and QM/MM MD for the degradation process of PET, accompanied by acylation and deacylation. We found that the distance of nucleophilic attack was reduced from about 4.6 Å in the wild type to 3.8 Å in 4Mz, and the free energy barrier of 4Mz dropped from 14.3 kcal/mol to 7.1 kcal/mol at the acylation which was the rate-limiting step. Subsequently, the high efficiency and universality of the DPM strategy were successfully demonstrated in LCC, Est119, and BhrPETase enhancing the degradation activity of PET. Finally, the highest degradation rate of the pretreated commercial plastic bottles had reached to 73%. The present study provides insight into the molecular binding mechanism of PET into the PET hydrolases structure and proposes a novel DPM strategy that will be useful for the engineering of more efficient enzymes for PET degradation.
研究领域:
生物化学
·
环境科学
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DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 24
·4323
·
IF 5.1
Yazhen Liu,Jiaqi Huang,Ji Yu,Luxin Fu,Ronglong Huang,Jing Liu,Bailin Deng,You-Bao Zhong,Duanyong Liu,Haimei Zhao
(1) Background: Ulcerative colitis (UC) is a persistent inflammatory condition of the intestine, characterized by dysregulated T cell-mediated immune responses. Curcumin (CUR), a common food additive and health supplement, is noted for possessing anti-inflammatory and immunomodulatory properties. Nevertheless, the molecular mechanisms underlying its therapeutic effects remain incompletely elucidated. This research aims to investigate the therapeutic mechanisms of CUR in UC, focusing on its role in restoring T cell homeostasis by modulating TIGIT and Neuropilin-1 (NRP1). (2) Methods: We employed a DSS-induced murine colitis model, combined with network pharmacology, molecular docking, protein–protein interaction docking, molecular dynamics simulations, andinvitroassays with Jurkat T cells. (3) Results: CUR markedly ameliorated clinical manifestations and histopathology in DSS-treated mice, restoring the balance of T cell and memory T cell subsets. Computational predictions and experimental validation showed that CUR downregulated TIGIT and NRP1 expression in inflamed colonic tissue and directly inhibited their expression in activated T cellsinvitro. (4) Conclusions: This study reveals a novel immunoregulatory mechanism of this natural compound. These findings suggest CUR modulates TIGIT/NRP1 to inhibit excessive T cell activation and restore immune homeostasis in UC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 17
·7671-7681
·
IF 3.591
Fengping Peng,Wanxin Xu,Yunya Hu,Weijie Fu,Haozhen Li,Jingyuan Lin,Yafeng Xiao,Zhe Wu,Wei Wang,Chunhua Lu
Inspired by microorganisms in nature, a new type of waste-energy-driven catalytic film was explored for the first time using magnetically actuated artificial cilia. Due to its singular characteristic ciliary movement, the inner-motile catalytic film is capable of capturing waste mechanical energy from flows, which can unexpectedly drive electrochemical reactions in solutions. Based on this, the piezoelectric deposition of Ag nanoparticles was achieved for the first time, which makes Ag nanoparticles distribute more evenly on the body of ZnO nanowires, enhancing the catalytic performance dramatically. Moreover, the influence of Ag nanoparticles on the piezoelectric catalysis was also detected, which can boost the deeper understanding of the piezo-catalytic mechanism. In summary, the inner-motile strategy facilitates the realization of waste-energy-driven catalytic technology, providing a new working prototype for driving the piezo-chemical reaction.
研究领域:
材料科学
·
生物仿生学
·
催化化学
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DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Lin Wang,Chengchun Zhang,Zhenjiang Wei,Zhentao Xin
The accumulation of ice on surfaces will bring safety issues to various human activities. Researchers have been actively developing superhydrophobic surfaces (SHS) as good anti-icing materials. However, some limitations, such as high cost, complexity of preparation, and lack of sufficient durability in extreme environments, restrict their practical applications. Inspired by bronchial mucosa cilia structure and the superhydrophobic lotus leaf structure, we generated ordered magnetic microcilia arrays (MMA) surfaces within 1 min by a fast and controllable microhole assisted magnetic-induced microcilia self-growth method. Fluoride-free superhydrophobic MMA (SMMA) was prepared by impregnating MMA into hexadecyltrimethoxysilane (HDTMS) modified SiO2 solution. SMMA exhibits excellent static anti-icing performance, which can significantly delay the freezing of static droplets in supercooled environments. The SMMA surface still maintains excellent dynamic anti-icing performance at −30 °C after 100 times of supercooled droplet impact. Furthermore, SMMA shows anti-icing performance for up to 2 months at low temperatures (−18 °C). Due to the sensitive magnetic response and excellent bending properties of the cilia, the MMA and SMMA surfaces also demonstrate outstanding multifunctional droplet manipulation under a magnetic field. The MMA surface has the ability to vertically capture and release droplets. The SMMA can achieve horizontal transport of droplets, mixing and microchemical detection, antigravity droplet transport in an 8° inclined array, and even complex objects can be easily transported. More importantly, the SMMA surface exhibits outstanding mechanical durability and chemical stability. It provides insights into the preparation of integrated anti-icing and droplet manipulation surfaces by using a simple green and low-cost method.
研究领域:
材料科学
·
表面工程
·
仿生学
·
防冰技术
·
液滴操控
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Qing-Song Huang,Zheng-Fei Yan,Xiao-Qian Chen,Yan-Yi Du,Juan Li,Zhan-Zhi Liu,Wei Xia,Sheng Chen,Jing Wu
Polyethylene terephthalate (PET) is a general plastic that produces a significant amount of waste due to its non-biodagradable properties. We obtained four bacteria ( Stenotrophomonas pavanii JWG-G1, Comamonas thiooxydans CG-1, Comamonas koreensis CG-2 and Fulvimonas soli GM-1) that utilize PET as a sole carbon source through a novel stepwise screening and verification strategy. PET films pretreated with S. pavanii JWG-G1 exhibited weight loss of 91.4% following subsequent degradation by Thermobifida fusca cutinase (TfC). S. pavanii JWG-G1 was able to colonize the PET surface and maintain high cell viability (over 50%) in biofilm, accelerating PET degradation. Compared with PET films with no pretreatment, pretreatment with S. pavanii JWG-G1 caused the PET surface to be significantly rougher with greater hydrophilicity (contact angle of 86.3 ± 2° vs. 96.6 ± 2°), providing better opportunities for TfC to contact and act on PET. Our study indicates that S. pavanii JWG-G1 could be used as a novel pretreatment for efficiently accelerating PET biodegradation by TfC.
研究领域:
环境科学
·
微生物学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
Nature Biomedical Engineering
·
2025
·
IF 26.6
Wei Xinwei,Wang Hanlin,Wang Yanfang,Zhang Wentao,Chen Changming,Li Kaihui,Han Licheng,Rufo Joseph,Xu Jianchang,Yao Yuejun,Huang Yingqi,Zhang Tianyu,Liu Xiangsheng,Gao Jianqing,Wang Ping,Xu Chao,Huang Tony Jun,Wang Jinqiang,Gu Zhen
Hair cells in the human ear contain cilia of varying lengths that sense varied acoustic signals. Here, inspired by this, we report an artificial cilia-based sound-decoding device capable of directly recognizing and responding to sound frequencies without relying on electricity and algorithms. We create 3D-printed micrometre-sized (40–200 μm) artificial cilia-based arrays with varying length-to-diameter ratios (30–100) that can sense and decode sound frequency signals (100–6,000 Hz), including piano music and human voices, on the basis of acoustic resonance. The artificial cilia can also vibrate accordingly in water to initiate subsequent tasks such as controlling drug release profiles of two distinct therapeutics (insulin and glucagon) in an acoustic-frequency-responsive manner to treat type 1 diabetic mice. This cochlear cilia-inspired device holds potential for broad applications such as recognizing complicated physiological sounds and performing various tasks in personalized voice interactions.
研究领域:
生物医学工程
·
纳米医学
·
转化医学
·
药物递送
·
声学传感
·
再生工程
·
分子诊断
✓ 已验证
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 26
·2101143
·
IF 18.808
Shuang Xia,Yukun Chen,Jinfeng Tian,Jinfeng Shi,Chengzhen Geng,Huawei Zou,Mei Liang,Zhibo Li
Gecko-inspired microfibrillar adhesives have achieved great progress in microstructure design and adhesion improvement over the past two decades. Space applications nowadays show great interest in this material for the characteristics of reversible adhesion and universal van der Waals interactions. However, the impact of harsh environment of space on the performance of microfibrillar adhesives, especially the extreme low temperature, is rarely addressed. Herein, microfibrillar adhesives fabricated by phenyl containing polydimethylsiloxane (p-PDMS) elastomers with superior low-temperature reversible adhesion is proposed. p-PDMS elastomers are synthesized through one-pot anionic ring-opening copolymerization, and the resulting elastomers become non-crystallizable with excellent low-temperature elasticity. Low-temperature adhesion tests demonstrate that the adhesion strength of microfibrillar adhesives fabricated by p-PDMS elastomers can be well maintained to as low as −120 °C. In contrast, the adhesion strength of pure PDMS microfibrillar adhesive reduces more than 50% below its crystallization temperature. The low-temperature cyclic adhesion tests further demonstrate that p-PDMS microfibrillar adhesives exhibit superior reversible adhesion compared to that of PDMS microfibrillar adhesives, owing to the sustainable conformal contact and even distribution of loads over repeated cycles. This study provides a new fabrication strategy for microfibrillar adhesives, and is beneficial for the practical application of microfibrillar adhesives.
研究领域:
高分子材料学
·
仿生工程
·
低温物理学
·
航天器材料
·
界面科学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Aoyu Fan,Yunyan Zhang,Yunpeng Li,Wei Meng,Fan Wu,Wei Pan,Zhongliang Ma,Wei Chen
Prostate cancer (PCa) is one of the most common malignancies for male individuals globally. Androgen deprivation therapy (ADT) initially demonstrated significant efficacy in treating PCa; however, most cases of PCa eventually progress to castration-resistant prostate cancer (CRPC), which becomes increasingly challenging to manage. Notably, the loss or disruption of primary cilia in PCa cells may play a critical role in the progression of the disease, and there are no reports on the role of circular RNAs in ciliogenesis. Thus, this warrants further investigation.In this study, key circular RNAs linked to prostate cancer progression, and enzalutamide resistance is identified. Specifically, it is found that hsa_circ_0005185 interacts with OTUB1 and RAB8A, serving as a molecular scaffold. Hsa_circ_0005185 mediates the binding of the deubiquitinase OTUB1 to RAB8A, resulting in the deubiquitination of RAB8A. Consequently, the stable expression of RAB8A promotes the regeneration of primary cilia and enhances the production of GLI3R, an inhibitory factor in the Hedgehog signaling pathway, thereby suppressing AR activity and slowing the progression of CRPC.
研究领域:
肿瘤学
·
分子生物学
·
癌症遗传学
·
信号转导
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）