Food & Function
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2021
·Vol. 13
·期 2
·880-890
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IF 5.396
Yana Zhao,Huizhen Li,Zhijun Zhang,Zhiqing Ren,Fuhan Yang
Polyphenols exhibit potential functional activities, especially rosmarinic acid (RosA) and caffeic acid (CafA). In this study, two different methods, ultrasonic-assisted ethanol extraction (60%) and ultrasound-assisted cellulase (≥15 000 Ug−1, 2%) hydrolysis, were used for the extraction of the total phenolics from 44 species of Perilla frutescens. The Folin–Ciocalteu method of detection showed that the content of the total phenolics extracted by cellulase hydrolysis was the highest and attained up to 28.00 mgGAE per gextracts for ZB1. Continuously, the extracts were purified using XDA-8 macroporous resin and medium-pressure liquid chromatography (MPLC), and the content of the total phenolics improved to 66.62 mgGAE per gextract. A high-performance liquid chromatography (HPLC) assay showed that the total polyphenols were mainly composed of gallic acid, caffeic acid, rosmarinic acid, luteolin and apigenin. Besides, a sequential XDA-8 macroporous resin combined with high-speed counter-current chromatography (HSCCC)/MPLC system was established for the simultaneous isolation and preparation of RosA (purity 98.29%) and CafA (purity 97.01%) from the extracts. Furthermore, the antibacterial activities of the total polyphenols were evaluated by the disc diffusion method and scanning electron microscopy (SEM) observation. The results verified that the total polyphenols had effective antibacterial activity on three kinds of bacteria including E. coli, S. aureus, and B. subtilis in a concentration-dependent manner. All of these results demonstrated that the ultrasound-assisted cellulase hydrolysis extraction of the total polyphenols and the proposed three-step separation of RosA and CafA gave high yields and good purity, and they exhibited effective antibacterial ability.
研究领域:
植物化学
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天然产物研究
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药理学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Weijie Lin,Dengwen Ning,Dezhong Xu,Shuai Wu,Qiuxia Zou,Ajoy Kanti Mondal,Fang Huang
This study compares the behavior of combined and free lignin on the cellulose enzymatic hydrolysis (EH). The combined lignin refers to the lignin naturally existed in the lignocellulose and the free lignin refers to the cellulolytic enzyme lignin. Three kinds of lignocelluloses (i.e., Moso bamboo, horsetail pine and aspen) are chosen for the research. Lignin is mainly biosynthesized of guaiacyl (G), syringyl (S), and p -hydroxyphenyl (H) structural units. The HSQC NMR analysis shows that the free lignin of Moso bamboo, horsetail pine and aspen are mainly composed of G-S-H, G-H and G-S type lignin units, respectively. Furthermore, a blood glucose meter (BGM), UV and fluorescence spectrophotometer are used to determine the cellulose conversion, enzyme adsorption and fluorescence intensities in the EH. It shows that the combined lignin has a weaker inhibitory effect on the EH breakdown than the free lignin. The influence of EH follows the order of Moso bamboo > aspen > horsetail pine. This study can be applied to the development of theoretical guidelines for mitigating the residual prohibition of lignin in the lignocellulose EH.
研究领域:
生物化学
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植物学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Yuanyuan Xia,Jingshun Zhuang
Green and sustainable pretreatment strategies are critical for the efficient utilization of lignocellulose. In this study, a novel ternary deep eutectic solvent (DES) consisting of choline chloride (ChCl), triethylene glycol (TEG), and oxalic acid (OA) was proposed to effectively fractionate and convert wheat straw into fermentable sugar. The ternary DES pretreatment showed remarkable pretreatment performance achieving 65.2 % delignification and 76.3 % hemicellulose removal under mild conditions. After 72 hours of enzymatic hydrolysis, a high glucose yield of 90.6 % was obtained, indicating a near-complete conversion of cellulose to monosaccharides. The structural properties of regenerated lignin samples were investigated by two-dimensional heteronuclear single-quantum coherence (2D-HSQC) and gel permeation chromatography (GPC). The regenerated lignin retained a well-preserved structure. Notably, after five recycling cycles of the DES, delignification remained at 51.2 %, with a glucose yield of 78.9 %. These findings suggest that the proposed ternary DES is a promising solvent for economically viable biomass conversion processes.
研究领域:
生物能源
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化学工程
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环境科学
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DOI
高影响因子
CELLULOSE
·
2014
·Vol. 21
·期 5
·3361-3369
·
IF 3.033
Lou Hongming,Lai Huanran,Wu Shun,Li Xiuli,Yang Dongjie,Qiu Xueqing,Huang Jinhao,Yi Conghua
The effects of long-chain fatty alcohols (LFAs) on the enzymatic hydrolysis of crystalline cellulose by two commercial Trichoderma reesei cellulase cocktails (CTec2 and Celluclast 1.5L) were studied. It was found that n -butanol inhibited the enzymatic hydrolysis, but n -octanol, n -decanol and n -dodecanol had strong enhancement on enzymatic hydrolysis of crystalline cellulose in the buffer pH range from 4.0 to 6.0. LFAs can increase the hydrolysis efficiency of crystalline cellulose from 37 to 57 % at Celluclast 1.5L loading of ten filter paper units (FPU)/g glucan. LFAs have similar enhancement on the enzymatic hydrolysis of crystalline cellulose mixed with lignin or xylan. The enhancement of LFAs increased with the decrease of the crystallinity index. LFAs not only enhanced the high-solid enzymatic hydrolysis of lignocellulose, but also improved the rheological properties of high-solid lignocellulosic slurries by decreasing the yield stress and complex viscosity. Meanwhile, LFAs can improve the enzymatic hydrolysis of cellobiose to glucose, especially at low cellulase loading.
研究领域:
生物化学
·
可再生资源与环境
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Zhaoyi Li,Zhen Sun,Guan Zhang
Solar reforming cellulosic biomass into hydrogen is an attractive research topic for sustainable biomass waste utilization and renewable energy development. To exert the advantages of enzymatic catalysis and heterogeneous photocatalysis , a glass fiber membrane integrated process that combines enzymatic hydrolysis of cellulose with sacrificial photocatalytic H 2 production from water under mild condition has been proposed as an example of integrating enzymatic catalysis and heterogeneous photocatalysis. Specifically, a low−cost Cu 0.5 Ni 0.5 −TiO 2 photocatalyst has been developed with presenting remarkable H 2 production performance even comparable with Pt−TiO 2 under UV light irradiation. The synergistic effect of Cu and Ni co−deposition onto TiO 2 has been found to improve the photocatalytic H 2 production. The condition for enzymatic hydrolysis of cellulose to generate glucose has been optimized in terms of reaction temperature, solution pH, types of cellulase and inorganic ions in order to obtain higher yields of glucose. To integrate the enzymatic catalysis and photocatalysis together, the glass fiber membrane with superior glucose penetration capability has been screened out. Lastly, the coupling of photocatalytic H 2 production from water based on the Cu 0.5 Ni 0.5 −TiO 2 photocatalyst and enzymatic hydrolysis of cellulose has been quantitively evaluated in both mixed and membrane−separation systems. The membrane−separation system can avoid the depletion of cellulase activity induced by photocatalytic oxidation , and thus presents higher H 2 production efficiencies with apparent quantum efficiency of 3.07 % at 365 ± 10 nm irradiation in initial 5 h. This work demonstrates that inorganic membrane integrated enzymatic catalysis and photocatalysis can be a powerful tool for different potential applications.
研究领域:
环境科学
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生物化学
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材料科学
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产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 4
·624
·
IF 4.7
Kuimin Yang,Jieqiong Yao,Kaixin Shi,Chenxi Yang,Yang Xu,Peipei Zhang,Siyi Pan
To improve the application potential of pomelo peel insoluble dietary fiber (PIDF) in emulsion systems, acetylation (PIDF-A), cellulase hydrolysis (PIDF-E), and wet ball milling (PIDF-M) were investigated in this paper as methods to change the emulsification properties of PIDF. The impact of the methods on PIDF composition, structure, and physicochemical properties was also assessed. The results demonstrated that both acetylation modification and cellulase hydrolysis could significantly improve the emulsification properties of PIDF. The emulsions stabilized with PIDF-A and PIDF-E could be stably stored at 25 °C for 30 d without phase separation at particle concentrations above 0.8% (w/v) and had higher storage stability: The D4,3increments of PIDF-A- and PIDF-E-stabilized emulsions were 0.98 μm and 0.49 μm, respectively, at particle concentrations of 1.2% (w/v), while the storage stability of PIDF-M-stabilized emulsion (5.29 μm) significantly decreased compared with that of PIDF (4.00 μm). Moreover, PIDF-A showed the highest water retention capacity (21.84 g/g), water swelling capacity (15.40 mL/g), oil retention capacity (4.67 g/g), and zeta potential absolute (29.0 mV) among the PIDFs. In conclusion, acetylation modification was a promising method to improve the emulsifying properties of insoluble polysaccharides.Keywords:pomelo peel insoluble dietary fiber;acetylation;cellulase hydrolysis;wet ball milling;emulsifying properties
研究领域:
食品科学
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化学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wenhong Gao,Jiajing Liu,Peilin Zhang,Xin-an Zeng,Zhong Han,Yongxin Teng
In this study, pectin extracted from pomelo peel was investigated using three different combination methods of pulsed electric field (PEF) and cellulase. Three action sequences were performed, including PEF treatment followed by enzymatic hydrolysis, enzymatic hydrolysis followed by PEF treatment, and enzymatic hydrolysis simultaneously treated by PEF. The three corresponding pectins were namely PEP, EPP and SP. The physiochemical, molecular structural and functional properties of the three pectins were determined. The results showed that PEP had excellent physiochemical properties, with the highest yield (12.08 %), total sugar (80.17 %) and total phenol content (38.20 %). The monosaccharide composition and FT-IR analysis indicated that the three pectins were similar. The molecular weights of PEP, EPP and SP were 51.13, 88.51 and 40.00 kDa, respectively. PEP showed the best gel properties, emulsification stability and antioxidant capacity among the three products, due to its high galacturonic acid and total phenol content, appropriate protein and low molecular weight. The mechanism of PEF-assisted cellulase hydrolysis of pomelo peel was also revealed by SEM analysis. These results suggested that PEF pretreatment was the best method, which not only improved the efficiency of enzymatic extraction, but also reduced resource waste and increased financial benefits.
研究领域:
食品科学
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化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2024
·
IF 3.1
Yang Yu,Ma Xueliang,Wang Manzhu,Ji Xinyi,Li Long,Liu Ziyu,Wang Jiangyao,Ren Yujin,Jia Lili
In this study, γ-butyrolactone/water (GBL/H 2 O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters—reaction time, temperature, and H 2 SO 4 concentration—were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H 2 O with 100 mM H 2 SO 4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H 2 O with 150 mM H 2 SO 4 at 120 °C for 1 h). Acknowledging the cellulase’s limited hydrolysis efficiency, a dual-enzyme scheme using a low cellulase dosage (10 FPU/g) supplemented with β-glucosidase or xylanase was tested, enhancing hydrolysis of CS pretreated under low temperature–long duration and high temperature–short duration conditions, respectively. Optimum sugar release was obtained from CS pretreated with GBL/H 2 O and 150 mM H 2 SO 4 at 120 °C for 1 h, achieving 98% glucan and 82.3% xylan conversion, compared with 53.9% and 17% of glucan and xylan conversion from untreated CS. GBL/H 2 O pretreatment outperformed other binary systems in literature, achieving the highest sugar conversions with lower enzyme loading. These results highlight the potential of GBL/H 2 O pretreatment for efficient biomass conversion, contributing to the goals of the green economy.
研究领域:
生物能源
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生物质转化
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2017
·
IF 5.951
Qiang Yu,Yunxuan Wang,Wei Qi,Wen Wang,Qiong Wang,Shixiang Bian,Yinping Zhu,Xinshu Zhuang,Zhongming Wang,Zhenhong Yuan
Traditional liquid hot water pretreatment (LHWP) has a high water consumption, a high reaction temperature, and low lignin removal, making it unsuitable for industrial applications of biomass conversion. In this study, we developed a new phase-exchange solvent pretreatment (PESP) based on the varied phase composition of furfural (FF)-water at different temperatures. Substitution of water with FF had no significant influence on xylan hydrolysis, but it improved the mass transfer performance. At the optimum conditions of 180 °C, an FF:water ratio of 30:70 (v/v), a solid:liquid ratio of 1:8 (w/v), and 0.2 wt % sulfuric acid for 30 min, the PESP of energy sorghum achieved a 74.98% total xylose yield and removed 85.08% of the lignin, while there was a selective distribution of sugar and lignin in the aqueous and organic phases, respectively. Moreover, 99.58% of the cellulase enzyme digestibility and 94.02% of the total sugar recovery were achieved after 72 h. This unusually high enzymatic digestibility could be attributed to the physicochemical changes in the substrate after the pretreatment. When compared with a traditional LHWP (5% solid loading), the water consumption decreased by approximately 72% and the lignin removal increased by 60.74%. These results demonstrate that PESP is a promising technology for biorefining lignocellulosic biomass with high efficiency and low energy consumption.
研究领域:
生物工程
·
可再生能源
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Houle Xiang,Yuqing Zhang,Shuo Zhang,Jingwei Kou,Hui Wang,Fengxia Zou,Wei Zhuang,Pengpeng Yang,Jinglan Wu,Hanjie Ying
Cell disruption is essential for extracting intracellular proteins from fungal biomass. However, its effects on the structural and functional properties of proteins remain insufficiently understood. This study used Cordyceps militaris as a model to evaluate three cell disruption techniques—high-pressure homogenization (HPH), ultrasonication (US), and cellulase hydrolysis—for extracting C. militaris protein isolates (CMPI). All methods significantly improved protein extraction compared to alkaline extraction alone, with HPH yielding the highest rate (70.75 %, p < 0.05). Structural analysis revealed a decrease in α-helix and β-sheet content, along with increased β-turns, random coils, surface hydrophobicity, and modifications in disulfide–sulfhydryl balance. CMPI-HPH and CMPI-US showed superior water-holding capacities (6.23 and 5.95 g/g) and oil-holding capacities (9.97 and 7.70 g/g), as well as enhanced foaming (69.57 % and 65.22 %) and emulsifying activities (32.07 and 31.13 m 2 /g). CMPI-HPH also exhibited the highest foaming stability (87.50 %). In vitro digestibility was significantly greater in CMPI-US (80.05 %) and CMPI-HPH (71.71 %) than in the control ( p < 0.05). These results demonstrate that cell disruption, especially HPH, effectively improves protein extraction efficiency, structural flexibility, and techno-functional properties, offering new potential for the sustainable application of fungal proteins in food systems.
研究领域:
食品科学
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生物技术
·
真菌学
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蛋白质工程
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结构生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yao Li,Jingyi Han,Otu Phyllis Naa Yarley,Yujin Wang,Yang Wang,Ao Zhang,Xingyu Fan,Cunshan Zhou,Weiqiao Lv
Dried shiitake mushrooms offer rich nutritional value and unique sensory properties, prompting further investigation. The effects of different drying techniques (hot air drying (HAD), infrared hot air drying (IRHAD), pulsed vacuum drying (PVD), vacuum freeze drying (VFD), and natural drying (ND)) combined with enzymatic hydrolysis on the release of flavor compounds and nutrients from shiitake mushrooms were explored. The combination of HAD with cellulase hydrolysis yielded notably high levels of umami amino acids (5.4723 ± 0.1501 mg/g) and 5′-nucleotides (4.0536 ± 0.0062 mg/g), and superior volatile flavors. Combined with cellulase hydrolysis, IRHAD achieved the highest level of total sugars (6.57 ± 0.34 mg/mL), VFD resulted in the greatest soluble protein content (153.21 ± 0.23 μg/mL), PVD yielded the highest total phenolics content (93.20 ± 0.41 μg GAE/mL), and ND produced the maximum reducing sugar content (5.79 ± 0.13 mg/mL). This study addresses crucial gap in the post-drying processing of shiitake mushrooms, offering valuable insights for further product development of shiitake mushrooms.
研究领域:
食品科学
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营养学
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酶学
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产后技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）