BIOMASS & BIOENERGY
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2025
·
IF 5.8
Yixin Sun,Qi Sun,Yingying Hou,Chunying Li,Xiaomei Hu
Filamentous fungi are key producers of cellulolytic and hemicellulolytic enzymes (CH enzymes), which play pivotal roles in lignocellulosic biomass conversion. Although several Talaromyces sp. have demonstrated the ability to produce CH enzymes, the enzymatic potential and regulatory mechanisms of Talaromyces endophyticus remain poorly understood. This study to systematically investigates the lignocellulose-degrading potential of T . endophyticus , providing new insights into its regulatory networks. The novel strain T . endophyticus NEAU-6 exhibited high CH enzymes activities when cultured on corn stover. Notably, enzymatic hydrolysis of alkali-pretreated corn stover using a crude CH enzyme loading of only 10 FPase/g of substrate resulted in a glucose yield of 70.2 %, highlighting its efficiency at a relatively low enzyme dosage. Structural and compositional characterization confirmed efficient degradation of the biomass. Full-length and comparative transcriptomic analyses uncovered 54 upregulated CH enzyme genes and a regulatory network involving 11 canonical transcription factors and developmental regulators, alongside 15 sugar transporters potentially mediating carbon sensing and uptake. These findings elucidate the regulatory network of lignocellulose degradation in T. endophyticus and highlight its potential as a candidate for further development in enzyme production and biomass conversion.
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JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Liu Yuchong,Zhao Yu,Yang Wantai,Wang Guan,Zhao Changwen,Liu Y.,Zhao Y.,Yang W.,Wang G.,Zhao C.
Biomass resources represent a sustainable alternative to fossil-based feedstocks for energy and value-added chemical production, and their utilization hinges largely on cellulase-catalyzed glucose conversion. Various immobilization strategies have been developed with the aim of cellulase recyclability. However, these strategies suffer from inadequate interactions between the substrate and immobilized cellulases, as well as poor recyclability. To overcome these drawbacks, we developed a hierarchical polymer brush architecture on polypropylene fibers through sequential photoinduced grafting and surface-initiated atomic transfer radical polymerization. This platform features hydrophilic polymer segments followed by reactive epoxy-functionalized brushes for covalent cellulase immobilization, enhancing substrate-enzyme interactions and cellulase stability against environmental alternations (especially harsh conditions). Importantly, the immobilized biocatalyst maintained 60% initial activity through six cycles in sodium carboxymethyl cellulose hydrolysis. This fiber-grafted block copolymer platform demonstrates an efficient strategy for cellulase immobilization, which opens up a new avenue for high-performance enzyme immobilization in industrial biocatalysis. Graphical abstract
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Advanced Science
·
2026
·
IF 14.1
Rui Zhang,Tongfang Jing,Leiming He,Kaidi Cui,Yuying Song,Xuewei Mao,Lidong Cao,Lin Zhou
Translating dynamic liquid‐liquid phase separation into robust solid‐state architectures remains a challenge in materials engineering. Here, we report a composition‐driven interfacial assembly strategy that couples supramolecular coacervation with metal‐phenolic coordination to engineer tunable fungicide carriers. By modulating the mass ratio between tea polyphenols (TP) and non‐ionic surfactants, we generate fluid templates that are kinetically trapped via pH‐triggered iron complexation. This one‐pot protocol allows for the precise regulation of the micro‐to‐nano population ratio, effectively addressing the dimensional mismatch between soil retention and systemic uptake. The resulting architecture achieves a functional division: microcapsules function as stationary reservoirs for rhizosphere protection, while nanocapsules act as mobile vectors for systemic curative action. Furthermore, the metal‐phenolic shell exhibits pathogen‐responsive disassembly upon exposure to fungal virulence factors, including oxalic acid and cellulases secreted by Fusarium pathogens. Validated in a peanut root rot model, this system demonstrates improved spatiotemporal efficacy and reduced aquatic toxicity toward zebrafish compared to commercial formulations. Consequently, this work presents a versatile methodology for structuring dynamic liquid interfaces based on TP‐surfactant supramolecular interactions, offering a potent solution for precision agriculture through the controlled solidification of supramolecular assemblies.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Mohan Li,Chenhao Jiang,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Sameh Awad,Jianquan Kan
First evidence of restricted-release, matrix-bound AFB1 in PPC was proved. • Protein binding is the primary reason for AFB1 hide in PPC. • Matrix hydrolysis or force dissociation effectively releases bound AFB1. • Vitro digestion pretreatment hydrolyzed the matrix, improving detection accuracy. • Highlights critical exposure underestimation for AFB1 in protein-rich samples. Pressed peanut cake (PPC) is a valuable plant protein resource but is highly susceptible to aflatoxin B1 (AFB1) contamination. Its complex matrix can complicate the accurate analysis of AFB1. Digesting the samples in vitro prior to quantification resulted in a significant improvement in AFB1 detection value (25.81–39.40%) compared with untreated samples. Ultrafiltration analysis confirmed that conventional extraction solvents are susceptible to severe interference from the matrix. Molecular docking demonstrated that AFB1 can form complexes with the matrix. Protein hydrolysis leads to significantly higher detection values than starch or cellulose hydrolysis, a phenomenon attributed to the dominant role of protein in binding the toxin. Peanut protein entraps and locks AFB1 through synergistic hydrophobic and hydrogen bonding interactions, forming restricted matrix-bound AFB1. The study elucidated on the formation/ dissociation mechanism of matrix-bound AFB1 in PPC at the molecular level, emphasizing the underestimation of the actual exposure level of AFB1 in PPC. Download: Download high-res image (254KB) Download: Download full-size image
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ChemistrySelect
·
2026
·Vol. 11
·期 13
·e73121
·
IF 2.3
Zhijie Cheng,Longjun Han,Zhixuan Lv,Jiaxin Tian,Jian Gao,Li Zhang
Biomass feedstocks have attracted considerable research attention as promising renewable alternatives amid rising fossil fuel costs and increasing demand for sustainable energy. The invasive plant Spartina alterniflora poses significant ecological threats; however, its straw represents a potential feedstock for bio-based products, thereby supporting both ecological management and resource utilization. However, the intrinsic recalcitrance of lignocellulosic biomass limits its efficient conversion, necessitating pretreatment strategies to improve enzymatic digestibility. Hydrothermal and deep eutectic solvent (DES) pretreatments have emerged as promising approaches due to their environmental compatibility and cost efficiency. This study investigated the synergistic effect of sequential hydrothermal and DES pretreatment on enhancing the enzymatic saccharification of Spartina alterniflora straw. The DES system consisted of choline chloride and lactic acid at a molar ratio of 1:10. Sequential hydrothermal pretreatment at 180°C was first conducted, followed by DES treatment. This process achieved xylan and lignin removal rates of 83.89% and 72.02%, respectively, while retaining 62.97% of the cellulose fraction, enzymatic hydrolysis efficiency increased by 245.54% relative to untreated substrates. These results demonstrate the potential of this strategy for sustainable biomass valorization. Graphical The synergistic Hydrothermal and lactic acid-choline chloride DES pretreatment of Spartina alterniflora was investigated. FTIR, XRD, and SEM were employed to analyze the physicochemical properties of the material and the composition of cellulose, hemicellulose and lignin before and after pretreatment. The pretreated material was subjected to cellulase hydrolysis to produce a sugar platform base liquid, providing a viable approach for utilizing invasive Spartina alterniflora .
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Advanced Science
·
2026
·
IF 14.1
Yi-Ke Wang,Ming Wang,Min Liu,Yi-Jing Chen,Li-Li Xu,Jin-Ping Zhou,Fu-Bing Wang,Shi-Bo Cheng,Min Xie,Wei-Hua Huang
Patient-derived xenograft (PDX) models constructed by noninvasively accessible circulating tumor cells (CTCs) serve as pivotal and invaluable tools for oncology research and precision medicine. However, the rarity of CTCs in blood and their limited proliferation ability present significant challenges for establishing patient CTC-derived xenograft (CDX) models. In this study, a novel 3D cellulose scaffold is developed for efficient isolation and in situ culture of CTCs, with the aim of constructing CDX models. Characterized by a macroporous and flexible structure, the 3D cellulose scaffold achieves high-efficiency CTC capture while maintaining cell viability. Its excellent biocompatibility and permeability further promote the rapid and in situ growth of CTCs. Importantly, the self-supporting and biodegradable scaffold allows for direct implantation into immunodeficient mice without leaving any residual material. This 3D cellulose scaffold has been successfully employed to construct CDX models from breast cancer patients. The application of this platform may enhance our understanding of metastasis mechanisms and offer significant benefits for cancer therapy.
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Foods
·
2026
·Vol. 15
·期 5
·877
·
IF 6
Yuanhui Zhang,Nengpai Shi,Chenjie Yang,Binbin Wu,Kexin Zhang,Shengnan Lin,Xuemei Hou,Xiangyang Lin
The grinding ofTremella fuciformisis a critical step for its value-added processing and the efficient utilization of its functional components, significantly impacting product quality and process adaptability. This study investigated ultrafine grinding (UFG) as a mechano-physical strategy to improve product quality, systematically analyzing its impact on physical properties (particle size, powder characteristics, color), extraction efficiency, chemical composition, and rheological behavior compared to conventional grinding (CG). The results revealed that UFG treatment induced an extensive disruption of the matrix, reducing particle size by 91.8% (D90 = 18.18 μm) and significantly increasing specific surface area. Notably, this physical modification directly translated into enhanced processing performance. UFG powder exhibited reduced powder flowability, superior solubility and improved color brightness. This structural degradation proved beneficial for extraction, unlocking a substantially higher yield (60.98–66.48%). Concurrently, the aqueous extracts of UFG powder exhibited more fluid-like rheological characteristics. This study confirms the potential of UFG as an effective pretreatment for the intensive processing ofT. fuciformisand indicates its promising application in functional food development and the extraction of bioactive components.
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Applied Food Research
·
2026
·Vol. 6
·期 2
·102371
·
IF 5.4
Zhenzhu Yang,Qunqun Chen,Huiqin Chen,Junjie Sun,Jianting Yang
Dendrobium is famous for its edibility and medicinal uses, with polysaccharides as active ingredients. Optimal fermentation conditions were obtained through single factor experiments combined with response surface methodology. The results showed that the extraction of CDP (Crude Dendrobium polysaccharides) was up to 5.84% with the inoculum 1.0%, sucrose dosage 0.9%, fermentation broth pH 5.0 of fermentation condition. A homogeneous polysaccharides component, designated as DOP-1, was successfully isolated and purified from Dendrobium officinale stem. The structural characterization of DOP-1 was conducted utilizing ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), high-performance liquid chromatography (HPLC), nuclear magnetic resonance spectroscopy (NMR), and scanning electron microscopy (SEM). Structure analysis revealed DOP-1 is mainly made up of mannose and glucose, with a molecular weight of 5526 Da. The structure of DOP-1 is composed of alternating or segmented D-mannose and D-glucose connected by β - (1→4), and carries monosaccharide side chains at the C2 positions of the residues. DOP-1 showed antioxidant capacity by scavenging DPPH, ABTS and •OH radicals in vitro antioxidant experiments, with IC 50 values of 5.43, 8.62 and 5.88 mg/mL respectively. During in vitro digestion test, no significant differences ( P > 0.05) were observed in the contents of total sugars and reducing sugars of DOP-1 before and after digestion, whereas its antioxidant activity dynamically increased initially and then remained stable. After digestion, the scavenging rates of DOP-1 against DPPH, ABTS, and •OH radicals were 73.18%, 79.55%, and 78.41%, respectively. The results showed that DOP-1 exhibited good gastrointestinal tolerance and potential for prebiotic development.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Haitao Yang,Wei Liu,Chenxu Li,Xingru Wu,Junxia Yuan,Lan Yao
Lignin, a major component of plant cell walls, plays a critical role in structural support and stress resistance. Despite its importance, the transport and deposition dynamics of the glycosylated lignin monomer during lignification in living cells remain poorly understood, hindering advances in biomass utilization. To address this challenge, alkyne-labeled glycosylated lignin precursors (pGCAALK and CFALK) were synthesized by introducing propargyl groups at the ortho position of aromatic rings. These precursors were successfully incorporated into lignin polymers in flax (a herbaceous plant) and ginkgo (a gymnosperm), enabling the real-time tracking of lignification via fluorescent click chemistry. Quantitative imaging revealed that lignification initiates at cell corners and the middle lamella and then progressively extends into secondary cell walls. Distinct deposition patterns were observed: parenchyma cells exhibited continuous lignin accumulation, whereas fiber tracheids underwent rapid lignification, followed by cell death. Specialized pit structures displayed “tunnel-like” lignin deposition in longitudinal pits and unilateral patterns in transverse pits. In vitro synthesis of dehydrogenation polymers (DHP) and extraction of the cellulolytic enzyme lignin (CEL) from ginkgo confirmed the biocompatibility of labeled monomers. LC-MS analysis further demonstrated that alkynyl groups formed oxygen-containing cyclic structures without disrupting natural β-O-4 and β-5 lignin linkages. Application of this labeling method in biomass utilization indicated that lower overall fluorescence intensity correlates with more efficient lignin removal during pretreatment. These results provide new insights into the spatiotemporal dynamics of lignification and establish a bioorthogonal platform for lignin research, offering promising strategies for optimizing plant biomass in industrial applications.
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ACS Food Science & Technology
·
2025
·
IF 2.8
Chun-Min Ma,Xiao-Han Li,Xiao-Peng Wang,Chun-Li Song,Xin-Huai Zhao
Pumpkin pulp (PP) was treated with α-amylase, cellulase, and pectinase to produce an enzyme-hydrolyzed PP (EPP), which was used to replace 50 or 100% water usage in cake making. Results indicated that PP/EPP usage reduced the air holding capacity of cake batter by increasing batter density and decreasing bubble area fraction and bubble numbers, while their usage in Chiffon cake enhanced protein, ash, crude fiber, and β-carotene contents, decreased water and lipid contents, and promoted textural indices of Chiffon cake. Meanwhile, PP/EPP usage decreased cake volume, specific volume, and baking loss ratio, increased cake shrinkage ratio, and caused a higher stomatal density and lower porosity area fraction. Altogether, EPP at 50% usage had a slight effect on these indices. It was thus concluded that EPP was a potential candidate for cake formulas to replace 50% water and could enhance the healthy functions of Chiffon cake via increasing dietary fiber and carotenoid contents.
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INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Niuniu Deng,Qiang Li,Wenjie Wang,Gengsheng Ji,Kaiyuan Wang,Jingyi Wu,Yanliang Chu,Xitao Cao
Native lignocellulosic biomass is notoriously recalcitrant because of its highly ordered cellulose crystallites and the encapsulating lignin–hemicellulose matrix, which greatly limits enzymatic hydrolysis and subsequent bioconversion. To overcome this barrier, we set out to design a fully biomass‑derived, recyclable pretreatment solvent capable of selectively disrupting cellulose crystallinity under mild conditions. Here, we report a family of deep eutectic solvents (DES) constituted from polycarboxylic acids (Citric and Tartaric acids) and polyols (Ethylene glycol, Glycerol, Sorbitol) and evaluate their pretreatment efficacy for corn stover. Multiscale characterization by NMR, FT‑IR, XRD, and rheology elucidated the hydrogen‑bond network architectures, viscosity profiles, and their mechanistic roles in the structural transformation of stover cellulose. Among the tested systems, the Citric acid–Sorbitol (CA–Sor) DES featured an optimised hydrogen‑bond network and moderate viscosity (72.2 mPa·s), reducing cellulose crystallinity from 62.3 % to 34.8 % and boosting reducing sugar conversion rate to 89.10 % within 72 h. The resulting hydrolysate could be directly fermented to ethanol without detoxification, achieving an 84 % glucose‑to‑ethanol conversion efficiency. This study therefore furnishes a novel, efficient, and recyclable green pretreatment strategy for the high‑value biorefining of agricultural residues and highlights its attractive potential for industrial deployment.
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Food Bioscience
·
2025
·
IF 5.9
Chengyu Jin,Xiao Wang,Xianghe Meng,Qin Ye
The attenuation of atopic dermatitis (AD) by Camellia oil was investigated in Raw 264.7 macrophages and HaCaT keratinocytes cell lines in this paper. Results showed that phenolic-abundant Camellia oil (PACO) by the AEE method showed the lowest expression levels of pro-inflammatory cytokines, including TNF-α, IL-6 and IL-1β in LPS-induced Raw 264.7 cells, followed by the cold-pressed Camellia oil (CPCO), then the commercial refined Camellia oil (CRCO). This may be attributed to a higher content of polyphenols in PACO, particularly for typical phenolics such as caffeic acid, quercetin, kaempferol, kaempferol-3-O-[2-O-β-D-galactopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside (KGRG) and kaempferol-3-O-[2-O-β-D-xylopyranosyl-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside (KXR), which are associated with lower NO levels in model cells and higher antioxidant capacities in DPPH, ABTS, and FRAP experiments. In HaCaT keratinocytes, exposure to TNF-α/IFN-γ resulted in an 8-fold and 10-fold increase in CCL-17 and IL-33, compared with control , indicating skin inflammation occurred. Camellia oil treatment improved this inflammation, embodied by a significant restoration of skin inflammatory mediators, and this improvement is dose-dependent. High-dose PACO exhibited the best anti-skin inflammatory effects. The mRNA expression levels of MMP1 , MMP9 , MMP25 , VEGFC and TNFRSF6B were also significantly down-regulated after PACO treatment, indicating its potential for skin barrier protection. Moreover, 800 μg/mL PACO significantly alleviated the cell apoptosis rate and regulated the apoptosis-related biomarkers at gene expression and protein expression levels ( Bax , Bcl-2 and Caspase-3 ). Analysis results on the JC-1 ΔΨm and mitochondrial ROS indicated that mitochondrial damage was reversed after the Camellia oil treatment, which accounted for its alleviation effects on apoptosis related to inflammation. Overall, Camellia oil, rich in phenolics, exhibited great therapeutic potential for AD treatment.
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