HYDROMETALLURGY
·
2023
·
IF 4.7
Nian Zhang,Fujian Li,Haiyang Xue,Zhongzhi Wang,Sen Qiu,Bangyu Fan,Yanyan Cheng,Xiangping Zhang
Aluminum (Al) is often associated with rare earth resources, and the removal of Al 3+ from rare earth elements (REEs) is a difficult task due to its amphoteric properties. Aluminum ions (Al 3+ ) enrich in Sm 3+ -Gd 3+ solution with the traditional 2-Ethylhexylphosphoric acid mono-2-ethylhexyl ester (P507)-kerosene-HCl system, emulsifying the phases, polluting the rare earth products and reducing the separation efficiency. A readily industrialized ionic liquid (IL) was synthesized with methyltrioctylammonium chloride ([N1888]Cl) and industrial naphthenic acid (NA) for the purification of Al 3+ from GdCl 3 solution. Under the experimental conditions, the extraction properties of diluted [N1888][NA], diluted NA and saponified NA were comparatively investigated and the separation factors ( β Al/Gd ) of Al/Gd were 23.0, 3.5 and 1.3, respectively. The effects of the salting-out agent, extraction agent, pH value and phase ratio on metal extraction efficiency were optimized. Finally, the industrial GdCl 3 feed with a high Al 3+ impurity of 889 mg/L was lowered to 5 mg/L. The removal efficiency of Al 3+ was 99.4%, and the purity of Gd 3+ was 99.998%. It provides a potential method for Al 3+ purification from rare earth solutions with advantages of high efficiency, raw materials availability and ready industrialization.
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2015
·Vol. 39
·期 5
·566-574
·
IF 0.741
Wu Zun-Qiu,Yue Gui-Zhou,Zhu Qing-Ping,Jiang You-Jun,Tang Kai-Yu,Chen Hua-Ping,Yang Ze-Shen,Huang Qian-Ming
Oleuropein was extracted from olive leaves by ultrasonic-assisted method and purified by silica gel column chromatography. The sample was identified as oleuropein by UV, infrared, mass spectroscopy and nuclear magnetic resonance spectroscopy analysis. Oleuropein and total phenolic contents over a year were determined by high-performance liquid chromatography and Folin–Ciocalteu methods. The results showed that 13.52% of pure oleuropein was found with a high purity of 96.54% and purification efficiency of 78.49%. Oleuropein and total phenolic contents of different olive varieties were quite different, but both had similar trends over the year. Five methods, including DPPH (2,2-diphenyl-1-picrylhydrazyl), ABST (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), ferric reducing antioxidant power, total reducing power and nitrite-scavenging ability, were used to evaluate the antioxidant activities of oleuropein, and the IC 50 were 34.54 ± 0.14 μg/mL, 18.79 ± 0.82 μg/mL, 75.32 ± 1.83 μg/mL, 13.80 ± 0.68 μg/mL and 1.00 ± 0.08 mg/mL, respectively. Practical Applications This study provides information on the changes in contents including oleuropein and total phenolics in a year to help understand the relationship between content of oleuropein and various influencing factors. Currently, the metabolic pathways of oleuropein are not clear. These results provide information for the study of the metabolism of phenolic compounds in olive and promote research at the molecular level. Oleuropein occupies a large proportion of total polyphenols in olive and can be to be purified by silica gel column chromatography with high purification efficiency for industrial production and laboratory production. In addition, the comprehensive evaluation of activity in vitro shows that oleuropein is more useful than BHT (2,6-di- tert -butyl-4-methylphenol) for the application in food and human health.
研究领域:
植物化学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Microfluidics and Nanofluidics
·
2019
·Vol. 23
·期 4
·1-8
·
IF 2.437
Qin Weiwei,He Yang,Xiao Jinyi,Liang Shengwang,Wang Shumei,Li Paul C. H.,Sun Yue
Liquid–liquid solvent extraction is frequently used in the purification of active ingredients from plants, and repeated extraction by different solvents with different polarity is often demanded to improve purity. According to this demand, two kinds of laminar flow extraction were designed on the microfluidic chip, i.e. a three-phase laminar chip and a successive laminar flow chip, and compared with a current two-phase chip. P. ginseng was used as a model plant to demonstrate extraction performance, and ether and n -butanol were used as the degrease solvent and extraction solvent, respectively. The results demonstrated that the extraction efficiency of a successive laminar flow chip is higher than that of a three-phase laminar flow chip, and that of both chips are higher than that of a two-phase chip. The chip method provided a new way to extract active ingredients from plants, which should be easily automated and integrated with other units to develop a complex system for sample preparation and ingredient detection.
研究领域:
植物学
·
药物化学
·
微流控技术
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2018
·Vol. 10
·期 3
·435-439
·
IF 2.917
Xu Hongpan,Sun Tingting,Huang Yuanfu,Song Lian,Li Zhiyang,Wang Wei
Placing of affinity tags at desired position of the proteins has been widely used to facilitate protein separation. One of the most commonly method for protein separation is nickel ion (Ni2+) affinity chromatography. Complex steps and time consuming in nickel ion affinity
chromatography have caused high demand for alternative purification methods that allow direct and rapid separation of His-tagged proteins. In the present study, magnetic core–shell beads enriched with Ni2+-nitrilotriacetate (Ni-NTA) on their surface were prepared in this study
by precipitation polymerization, with a uniform size of about 10 μm. The presented Ni2+ amount was 2.23%, which endowed the spheres with excellent magnetic responsivity and dispersity. We compared the magnetic beads enrichment method and nickel ion affinity chromatography
method for extraction of His-tagged protein sample with different concentrations. The results revealed that, when compared with nickel ion affinity chromatography, extraction efficiency, purity of the extracted protein, and regeneration effort were much higher with the magnetic beads enrichment
method. By using magnetic beads enrichment method, samples could be separated within 20 s, and purified within 1 h, with unlimited flow velocity and sample capacity. In addition, the magnetic beads enrichment method was particularly promising for the separation of His-tagged proteins in less
samples.
研究领域:
生物化学
·
蛋白质组学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Guangying Sun,Zheng Zhang,Liying Yang,Jianming Jiang,Wu Yao,Le Pan,Long Chen,Changjiang Li,Zhaosheng Liu
In this work, a solute retention optimization method (SRO) was proposed to exploit the purification potential of two-dimensional liquid chromatography (2D-LC). According to our findings, the complementarity of 2D-LC correlates with some specific impurities. In the two methods used in 2D-LC, the retention order of these impurities and target compound is completely opposite. Taking full advantage of the complementarity is crucial to enhance the saturation capacity ( w max ) of 2D-LC by SRO. For the purpose of validating the effectiveness of SRO, a reverse-phase liquid chromatography (RPLC) coupled with hydrophilic interaction chromatography (HILIC) was developed to purify p -chlorobenzoic acid from substituted benzenes. By using the overloading effects of analytes as indicators, the w max of RPLC × HILIC was determined by the bisection method, and finally defined by the extremely high loading volume of 4.9 mL. A touch-peak separation of impurities and the target compound occurred precisely during the secondary separation. The effectiveness of SRO was also verified by the greater purification efficiency of RPLC × HILIC than that of HILIC × RPLC. Subsequently, a RPLC × RPLC method was developed by SRO to prepare the reference materials of caffeine from tea extracts. Only by an analytical C18 column, 15.6 mg of caffeine with the purity of 98.3% was obtained at once with the recovery up to 82.3%. However, without the aid of SRO, the purity rapidly decreased to 62.0%. Compared to other methods, SRO-based 2D-LC offers certain advantages in terms of purity, recovery, and the purification efficiency, suggesting that it is particularly effective in developing preparative 2D-LC facing complex matrices.
研究领域:
分析化学
·
色谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）