MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Zhengyuan Hong,Biao Yu,Jiachao Xiao,Hongyan Feng,Xiaowei Ma,Zhen Cheng,Lihong Bu
N-(2-(diethylamino)-ethyl)- 18 F-5-fluoropicolinamid ( 18 F-P3BZA) is a promising alternative probe for early diagnosis of malignant melanoma. Developing an efficient and timesaving method for 18 F-P3BZA purification is necessary because this procedure is able to remove the unreacted precursor and undesired radioactive substances in a short time so that reducing their impacts on imaging and also decreasing the 18 F-P3BZA loss caused by 18 F decay. Herein, we investigated a solid phase extraction-based method for 18 F-P3BZA purification by use of a single C18 light cartridge and found that eluting crude product-captured C18 light cartridge with 20 mL 0.01 M NH 4 HCO 3 and then 2 mL ethanol/0.01 M NH 4 HCO 3 (1/4, v/v) was capable of giving 18 F-P3BZA with a high radiochemical purity and an extremely low precursor content. Furthermore, this purification method allowed automated radiosynthesis of 18 F-P3BZA to be completed within 40 min, which was much faster than that of previous studies. In addition, this purification pathway was low cost and easily to be achieved, thus possessing potential to facilitate the universalness of 18 F-P3BZA.
研究领域:
医学
·
药学
·
核医学
✓ 已验证
DOI
高影响因子
Marine Drugs
·
2019
·Vol. 17
·期 1
·68
·
IF 3.772
Shangyong Li,Linna Wang,Xuehong Chen,Mi Sun,Yantao Han
Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer, cyanuric chloride. Based on the CHDS-modified affinity resin, a one-step affinity purification method was developed and optimized, and then applied to purify three typical glycoside hydrolase (GH) families: 46, 75, and 80 chitosanase. The three purified chitosanases were homogeneous with purities of greater than 95% and bioactivity recovery of more than 40%. Moreover, we also developed a rapid and efficient affinity purification procedure, in which tag-free chitosanase could be directly purified from supernatant of bacterial culture. The purified chitosanases samples using such a procedure had apparent homogeneity, with more than 90% purity and 10–50% yield. The novel purification methods established in this work can be applied to purify native chitosanases in various scales, such as laboratory and industrial scales.Keywords:chitosanases;adsorption analysis;affinity purification
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2016
·
IF 1.988
Zhaozhu Zheng,Shaozhe Guo,Yawen Liu,Jianbing Wu,Gang Li,Meng Liu,Xiaoqin Wang,David Kaplan
Silk fibroin protein was purified from Bombyx mori silkworm cocoons using a novel dialysis strategy to avoid fibroin aggregation and pre-mature formation of β-sheets. The degummed silk fibers were dissolved in Ajisawa’s reagent, a mixture of CaCl2–EtOH–H2O, that is less expensive than lithium bromide. The dissolved solutions were dialyzed against either water or urea solution with a stepwise decrease in concentration. When the steps of 4 M-2 M-1 M-0 M urea (referred to as silk-TS-4210) were adopted, the purified silk fibroin had smaller aggregates (<10 nm), similar average molecular weight (225 kDa) and a lower content of β-sheet (∼15%) compared to the sample processing methods (silk-TS-210, 10, 0) studied here. This outcome was close to the fibroin purified by the lithium bromide (silk-Li-0) method. Polyvinyl alcohol-emulsified silk microspheres generated using the purified solution had a similar size distribution and morphology when compared to lithium bromide dissolved solutions, while glycerol-blended silk films showed different mechanical properties. The silk-Li-0 generated films with the highest breaking strength (5.7 MPa ± 0.3) while the silk-TS-4210 had the highest extension at break (215.1% ± 12.5). The films prepared from silk-TS-4210 were cytocompatible to support the adhesion and proliferation of human mesenchymal stem cells, with improvements compared to the other samples likely due to the porous morphology of these films.
研究领域:
生物材料学
·
蛋白质化学
✓ 已验证
DOI
PubMed
高影响因子
Chemistry-An Asian Journal
·
2021
·Vol. 16
·期 4
·348-354
·
IF 4.568
Shengju Zhou,Ying Sui,Xiaotong Zhu,Xiaofeng Sun,Shuping Zhuo,Hongguang Li
Graphical The multicolor emission CDs have been successfully prepared through a one-pot pyrolysis of citric acid in formamide. Then, the precipitation method, dialysis and GPC were successively employed to purify the multicolor emission CDs. Recent years have witnessed a rapid development of carbon dots (CDs), due to their outstanding luminescence properties and excellent biocompatibility. However, the internal structure and photoluminescent (PL) mechanism of CDs are still the subject of considerable debate, which is due to the fact that reaction products usually contain mixtures of several CD fractions as well as molecular intermediate and side products. Therefore, careful purification of the CDs is significant for analysis of structure and luminescence mechanism. Here, multicolor emission CDs were prepared by a one-pot pyrolysis of citric acid in formamide. Then, the precipitation method, dialysis and gel permeation chromatography (GPC) are successively employed to purify the multicolor emission CDs. This post-treatment allowed us to compare the optical properties of CDs obtained by different separation methods and provide a valuable guidance for the purification of CDs.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
·
2020
·
IF 1.513
Xiaonan Guo,Qing Sun,Hualong Xi,Yue Zhang,Mengze Guo,Chenxing Zhang,Shidong Zhu,Tiejun Gu,Wei Kong,Yongge Wu
Streptococcus pneumoniae is a gram-positive bacterial pathogen causing invasive pneumonia, meningitis, otitis media , and bacteremia . Owing to the current pitfalls of polysaccharide and polysaccharide-conjugate vaccines, protein vaccines are considered promising candidates against pneumonia. Pneumococcal surface protein A (PspA) and pneumococcal surface adhesin A (PsaA) are virulence proteins showing good immunogenicity and protective effects against S. pneumoniae strains in mice. In this study, we expressed the fusion protein PsaA-PspA, which consists of PsaA and the N-terminal region of PspA family 1 and 2, in Escherichia coli . We describe a novel and effective method to purify PsaA-PspA using hydroxyapatite and two-step chromatography . After determining the optimal induction conditions and a series of purification steps, we obtained PsaA-PspA fusion protein with over 95% purity at a final yield of 22.44% from the starting cell lysate. The molecular weight of PsaA-PspA was approximately 83.6 kDa and its secondary structure was evaluated by circular dichroism . Immunization with the purified protein induced high levels of IgG antibodies in mice. Collectively, these results demonstrate that our purification method can effectively produce high-purity PsaA-PspA fusion protein with biological activity and chemical integrity, which can be widely applied to the purification of other PspA subclass proteins.
研究领域:
微生物学
·
免疫学
·
疫苗研究
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 17
·6651-6657
·
IF 1.938
Zhiyong Zhao,Suquan Song,Na Liu,Qinxiong Rao,Jiafa Hou,Sarah De Saeger,Aibo Wu
Food safety monitoring and toxicological research of mycotoxins are still in need of large quantities of high-purity deoxynivalenol (DON). To attain this purpose, a rapid, economical and reproducible purification method was developed for large-scale production of DON from rice culture inoculated with a DON-producing Fusarium graminearum strain JYH. The inoculated rice culture was first extracted with acetonitrile–water (84/16, v/v). The extracts were evaporated to dryness on a rotary evaporator after ethyl acetate partitioning and then dissolved in water followed by the final purification procedure through preparative high performance liquid chromatography and montmorillonite treatment. A combined approach of ultraviolet spectrometry (UV), ultra high performance liquid chromatography (UHPLC), mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy was applied for the multi-dimensional characterization of the target compound. As a result, the recovery of DON from the crude extract to the final product was up to 70%. An amount of 150 mg DON with the desirable purity of 98.93% could be obtained from 100 g of rice culture, which possessed identical immunochemical characteristics compared to a certified commercial DON standard. This proposed strategy might act as a valuable reference to obtain rather expensive compounds in a straightforward way.
研究领域:
食品安全
·
真菌学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2022
·Vol. 24
·期 1
·1-18
·
IF 2.533
Ning Tianxiang,Luo Yinyi,Liu Piao,Lu Anxian
In this study, a novel method called membrane separation–centrifugation technology is used to purify the AgNPs. The TGA results showed that the AgNPs washed in this method only contained a minimal amount of organic residues (< 5 wt.%). Moreover, the XRD results indicate that the cleaned AgNPs are pure single-crystal cubic phase Ag without other impurities. Furthermore, according to the results of SEM and TEM, the AgNPs cleaned by this technology are nanoscale with an average particle size of only 50 nm and dispersed evenly without agglomeration. The colloid made from AgNPs purified by this method reveals a good Zeta potential of about -34.7 mV, which indicates the excellent stability of AgNPs. After the ink made by these AgNPs is dried at 150℃ for 10 min, a really low electrical resistivity only about 10.7 × 10 –6 Ω·cm is obtained. These results confirm that the membrane separation–centrifugation technology can significantly improve the performances of the AgNPs ink, which can be used in printed electronics.
研究领域:
材料科学
·
纳米技术
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Junjun Yin,Duo Wang,Zhifeng Zheng,Yueyuan Ye,Shuirong Li,Xingyong Jia
Based on the characteristic that Ca2+ can react with 4-ethyl-2-methoxyphenol (EMP) to form a complexation with a phenol–calcium ratio of 4:1, a new extraction and purification method of EMP is developed for the first time in this work. At an optimum purification condition, 99.60% purity of EMP can be obtained through a reaction and decomposition operation. By combining a variety of characterizations, which consist of in situ Fourier transform infrared spectrometer (FTIR), nuclear magnetic resonance (NMR), inductively coupled plasma optical emission spectrometer (ICP-OES), gas chromatography–mass spectrometry (GC–MS)/flame ionization detector (FID), elemental analysis, and thermogravimetric analysis, the reaction mechanism of the coordination process is studied. It is demonstrated that there are three stages of the coordination reaction between Ca2+ and EMP. A neutralization reaction occurs in the first stage, while the second stage is a mixing reaction stage including neutralization and coordination reaction. When the reaction proceeds to the third stage, another coordination reaction occurs. Furthermore, phenol and ethanol are added as impurities in EMP. EMP with a purity of more than 99.50% can be obtained using this purification method. It confirms that this efficient method can achieve a good purification effect even for EMP solutions with complicated components.
研究领域:
化学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）