JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 101
·期 15
·6193-6201
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IF 3.639
Liuna Wei,Hengye Chen,Rui Liu,Shuo Wang,Tingkai Liu,Zikang Hu,Wei Lan,Yongjie Yu,Yuanbin She,Haiyan Fu
BACKGROUND Ethyl carbamate (EC) is a potentially toxic carcinogen produced during fermentation and storage of fermented foods, and many countries have set thresholds for its content in food. Therefore, sensitive, rapid and accurate detection of EC is meaningful to ensure the quality of fermented food. RESULTS This study introduces a CdTe quantum dots/nano-5,10,15,20-tetrakis (4-methoxyphenyl)-porphyrin (nano TPP-OCH 3 ) fluorescence sensor system detection of EC. The specificity of this sensing mainly relies on a photo-induced electron transfer and electrostatic force interaction between EC and nano TPP-OCH 3 . This sensor presented a linear range of 10 to 1000 μg L −1 ( R 2 = 0.9903) with a low detection limit of 7.14 μg L −1 . Meanwhile, the recovery (91.19–101.09%) and precision [relative standard deviation (RSD) = 0.64–3.05%] of the sensor for the analysis of fermented food (yellow rice wine, soy sauce, Chinese spirits, Pu-erh tea) samples were good and could meet the requirements of practical detection. Moreover, the detection results of fermented food (yellow rice wine, soy sauce, Chinese spirits, Pu-erh tea) samples by this sensor are basically consistent with those of high-performance liquid chromatography with fluorescence detector (HPLC-FLD). CONCLUSION This method was expected to provide a potential platform for sensitive and accurate detection of EC in food safety monitoring, which would provide knowledge of the flavor and quality related to fermented food. © 2021 Society of Chemical Industry.
研究领域:
食品安全
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分析化学
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
·
2022
·
IF 9.417
Li Chen,Xiang Li,Mengmeng Xiong,Yimei Zhao,Suyi Liu,Cao Li,Kai Wang
Photothermal therapy (PTT), a promising spatiotemporally controllable cancer treatment method, needs effective depth of laser irradiation to ensure the anticancer efficiency. Second near-infrared window (NIR-II) absorbing conjugated polymer (CP) nanomaterials are emerging candidates for a non-invasive treatment of cancer with considerations of safety. Herein, a new organic CP compound (Ppordpp) with the donor-acceptor structure of porphyrin and diketopyrrolopyrrole was synthesized, which showed efficient absorption extending in NIR-II and spontaneously self-assembled into NPs (∼190 nm), leading to emission quenching and facilitating nonradiative thermal generation. Photothermal conversion efficiency (PCE) of our Ppordpp NPs was up to 86.21 %, higher than most of reported PTT reagents. Under 940 nm (0.75 W/cm 2 ) laser irradiation for 10 min, the Ppordpp NPs showed dramatical PTT effects in vitro to kill 78 % 4T1 cancer cells with 40 μg/mL incubation, and in vivo to almost completely inhibition tumor growth by 200 μg/mL injection without obvious side effects. Our CP nanomaterials Ppordpp NPs with a D-A structure provide new alternatives for precise cancer through photothermal therapy with high PCE.
研究领域:
生物医学工程
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肿瘤学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Gaoqiong Deng,Shuo Wang,Hengye Chen,Lixue Ren,Ke Liang,Liuna Wei,Wanjun Long,Jian Yang,Lanping Guo,Xiaole Han,Yuanbin She,Haiyan Fu
Carbaryl is a typical carbamate pesticide that plays an essential role in agricultural production, but its residues cause serious harm to the environment and human health. Here, we developed a polychromatic colorimetric sensor based on ZnTPyP-DTAB peroxidase activity and gold nano-bipyramids (Au NBPs) etching to detect carbaryl. ZnTPyP-DTAB catalyzes the decomposition of H 2 O 2 to hydroxyl radicals, and Au NBPs are etched. The coordination of zinc and nitrogen in nanometer porphyrins was affected by the steric effects of carbaryl, which resulted in decreased activity of ZnTPyP-DTAB peroxidase. The detection limit of carbaryl was 0.26 mg/kg. The recoveries of carbaryl in reaal sample ranged from 91 % to 107% (RSD ≤ 0.7%). The sensor platform displayed a series of high-resolution multicolor variations of rainbow colors within the above concentration range. The rich color variation facilitates the acquisition of digital images. RGB value transformation combined with partial least squares regression model can accurately and quantitatively detect carbaryl in vegetables, fruits and Chinese medicinal materials.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Qin Wang,Qiaobo Yin,Yao Fan,Lei Zhang,Ying Xu,Ou Hu,Xiaoming Guo,Qiong Shi,Haiyan Fu,Yuanbin She
A high-sensitivity fluorescence visualization paper-based sensor is developed and used to achieve specific detection and analysis of three organophosphorus pesticides (OPPs: dimethoate , dichlorvos , and demeton) in a "Turn-off-on" detection mode. The fluorescence visualization paper-based sensor is established through combining double quantum dots (QDs) with high-activity nanoporphyrins (QDs-nanoporphyrin), realizing double nanometer signal amplification and producing different color change responses to these three OPPs. In particular, this approach is based on Partial least squares discriminant analysis (PLSDA) for fingerprint spectrum analysis of three kinds of organophosphorus pesticides in complex matrix (apple and cabbage). Therefore, different concentrations of pesticide residues can be quickly identified at the same time with 100% accuracy for both training set and prediction set. In summary, this method has high selectivity and stability, and thus provides a new approach for the identification of organophosphorus residues in complex systems.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Jiaying Zeng,Ting Zhang,Geyu Liang,Jingwen Mo,Jianxiong Zhu,Longhui Qin,Xiaojun Liu,Zhonghua Ni
Hexavalent chromium (Cr(Ⅵ)) is a significant environmental pollutant because of its toxic and carcinogenic properties and wide use in various industries. Hence, there is an urgent need to develop accurate and selective approaches to detect the concentration of Cr(Ⅵ) in agricultural and aquaculture products to help humans avoid potential hazards of indirectly taking in Cr(Ⅵ). In this work, we report a “turn off–on” fluorescent sensor based on citric acid coated, 808 nm-excited core–shell upconversion nanoparticles (CA-UCNPs) and self-assembled copper porphyrin nanoparticles (nano CuTPyP) for sensitive and specific detection of Cr(Ⅵ). Nano copper 5, 10, 15, 20-tetra(4-pyridyl)-21H–23H- porphine obtained by acid-base neutralization micelle-confined self-assembly method function as an effective quencher due to its excellent optical property and water solubility. Through electrostatic interactions, positively charged nano CuTPyP are attracted to the surface of negatively charged CA-UCNPs, which can almost completely quench the fluorescence emission. In the presence of Cr(Ⅵ), nano CuTPyP can discriminatively interact with Cr(Ⅵ) and form nano CuTPyP/Cr(Ⅵ) complex, which separates nano CuTPyP from CA-UCNPs and restores the fluorescence. The sensing system exhibits a good linear response to Cr(Ⅵ) concentration in the range from 0.5 to 400 µM with a detection limit of 0.36 µM. The sensing method also displays high selectivity against other common ions including trivalent chromium and is applied to the analysis of Cr(Ⅵ) in actual rice and fish samples with satisfactory results.
研究领域:
环境科学
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 19
·3360
·
IF 5.1
Yaqi Liu,Zhenli Cai,Yao Fan,Xingcai Wang,Meixia Wu,Haiyan Fu,Yuanbin She
Aimed at addressing the increasingly serious problem of adulteration in Xihu Longjing, a catechin-targeted nano-enhanced visual and fluorescent dual-mode sensor array was constructed by nano porphyrins and quantum dots (QDs) for the precise analysis of Xihu Longjing from adjacent origins. This sensor array realizes the quantitative analysis of catechin enantiomers in Xihu Longjing through the selective combination of sensing units. It can accurately identify adjacent Xihu Longjing teas with different grades and storage times and can precisely detect samples with a low proportion of adulteration. At the same time, the flavor quality and antioxidant performance of Xihu Longjing tea can also be quantitatively evaluated. The dual-mode sensor array design proposed in this study provides a new idea for detecting minor differences in food authenticity and has significant application value for quality control in the tea industry.
研究领域:
食品科学
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分析化学
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纳米生物技术
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茶叶质量控制
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分子识别
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传感技术
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天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 101
·期 6
·2552-2560
·
IF 2.614
Hengye Chen,Liuna Wei,Xiaoming Guo,Chengying Hai,Lu Xu,Lei Zhang,Wei Lan,Chunsong Zhou,Yuanbin She,Haiyan Fu
BACKGROUND The quality of tea is influenced by numerous factors, especially l- theanine, which is one of the important markers used to evaluate the sweetness and freshness of tea. Sensitive, rapid, and accurate detection of l -theanine is therefore useful to identify the grade and quality of tea. RESULTS A high-sensitivity, paper-based fluorescent sensor combined with chemometrics was established to detect l- theanine in tea water based on CdTe quantum dots / corn carbon dots and nano tetra pyridel-porphine zinc (ZnTPyP). To verify the reliability of this method, fluorescence spectra and fluorescence-visualized paper-based sensors were compared. The fluorescence spectrum method demonstrated a linear range of 1 to 10 000 nmol L −1 and a limit of detection (LOD) of 0.19 nmol L −1 . In the fluorescence-visualized paper-based sensors there was a linear range of 10–1000 nmol L −1 , and the LOD was 10 nmol L −1 . Partial least squares discriminant analysis (PLSDA) and partial least squares regression analysis (PLSR) were used successfully to determine l -theanine accurately in tea water with this approach. The accuracy of the PLSDA model was 100% both in the training set and the predicting set, and the correlation coefficient between the actual concentration and the predicted concentration was greater than 0.9997 in the PLSR model. CONCLUSION This fluorescence-visualized paper-based sensor, combined with chemometrics, could be applied efficiently to the practical analysis of tea water samples, which provides a new idea to ensure the flavor and quality of tea. © 2020 Society of Chemical Industry
研究领域:
分析化学
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食品科学
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化学传感器
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化学计量学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Haiyan Fu,Ou Hu,Yao Fan,Ying Hu,Jiahao Huang,Zhao Wang,Yuanbin She
Interests in the recognition of chiral amino acids have grown tremendously in recent years, particularly in the fields of food analysis and pharmaceutical studies. Herein, an innovative “on-off-on” fluorescent assay has been developed for the sensitive and selective determination of chiral Proline (Pro), Lysine (Lys), and Serine (Ser). This detection platform is constructed by exploiting the fluorescence quenching ability of chiral self-assembly zinc 5, 10, 15, 20-tetra(4-pyridyl)-21 H -23 H -porphine (ZnTPyP) to CdTe quantum dots (CdTe QDs). CdTe QDs can act as signal switches whose fluorescence signals can effectively quenched by chiral nano ZnTPyP and then restored in the presence of enantiomer amino acids. The coupling use of CdTe QDs and chiral nano ZnTPyP also permits quantitative analysis of d -Pro, d -Lys, l -Ser with detection limits of 4.46 × 10 −10 mol L −1 , 7.13 × 10 −11 mol L −1 , and 3.35 × 10 −11 mol L −1 , respectively. The approach is also suitable for the effective detection of the three amino acids in complex biological matrices. This rapid, robust and reliable strategy for recognizing enantiomer amino acids has not been reported. The proposed method can be a powerful and valuable technology in the areas of drug discovery and food chemistry.
研究领域:
分析化学
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生物技术
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药学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Weng Changmei,Li Guanhua,Zhang Dongdong,Duan Zhaoxia,Chen Kuijun,Zhang Jieyuan,Li Tao,Wang Jianmin
Acute lung injury (ALI) has high mortality and still lacks novel and efficient therapies. Zinc finger E-box binding homeobox 1 and 2 (ZEB1/2) are highly expressed in the early stage of ALI and are positively correlated with the progression of pulmonary fibrosis. Herein, we developed a nanoscale Zr(IV)-based porphyrin metal-organic (ZPM) framework to deliver small interfering ZEB1/2 (siZEB1/2) to alleviate early pulmonary fibrosis during ALI. This pH-responsive nano-ZPM system could effectively protect siRNAs during lung delivery until after internalization and rapidly trigger siRNA release under the mildly acidic environment of the endo/lysosome (pH 4.0–6.5) for transfection and gene silencing. Furthermore, the in vivo studies confirmed that this nano-ZPM system could anchor in inflamed lungs. Moreover, the ZEB1/2 silencing led to increased E-cadherin and decreased α-SMA levels. Overall, the nano-ZPM system was an excellent non-viral vector system to deliver siRNAs to alleviate early pulmonary fibrosis during ALI.
研究领域:
药学
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分子生物学
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纳米技术
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肺科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2024
·
IF 11.4
Yi Chen,Shan Xu,Shuang Ren,Jiyuan Zhang,Jinzhuan Xu,Yuxuan Song,Jianqing Peng,Shuai Zhang,Qianming Du,Yan Chen
Introduction The combination of a photosensitizer and indoleamine-2,3 dioxygenase (IDO) inhibitor provides a promising photoimmunotherapy (PIT) strategy for melanoma treatment. A dual drug delivery system offers a potential approach for optimizing the inhibitory effects of PIT on melanoma proliferation and metastasis. Objective To develop a dual drug delivery system based on PIT and to study its efficacy in inhibiting melanoma proliferation and metastasis. Methods We constructed a multifunctional nano-porphyrin material (P18-APBA-HA) using the photosensitizer-purpurin 18 (P18), hyaluronic acid (HA), and 4-(aminomethyl) phenylboronic acid (APBA). The resulting P18-APBA-HA was inserted into a phospholipid membrane and the IDO inhibitor epacadostat (EPA) was loaded into the internal phase to prepare a dual drug delivery system (Lip\EPA\P18-APBA-HA). Moreover, we also investigated its physicochemical properties, targeting, anti-tumor immunity, and anti-tumor proliferation and metastasis effects. Results The designed system utilized the pH sensitivity of borate ester to realize an enhanced-targeting strategy to facilitate the drug distribution in tumor lesions and efficient receptor-mediated cellular endocytosis. The intracellular release of EPA from Lip\EPA\P18-APBA-HA was triggered by thermal radiation, thereby inhibiting IDO activity in the tumor microenvironment, and promoting activation of the immune response. Intravenous administration of Lip\EPA\P18-APBA-HA effectively induced anti-tumor immunity by promoting dendritic cell maturation, cytotoxic T cell activation, and regulatory T cell suppression, and regulating cytokine secretion, to inhibit the proliferation of melanoma and lung metastasis. Conclusion The proposed nano-drug delivery system holds promise as offers a promising strategy to enhance the inhibitory effects of the combination of EPA and P18 on melanoma proliferation and metastasis.
研究领域:
药学
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癌症研究
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纳米技术
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）