SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Mei Du,Jingzhang Liu,Bang Huang,Qiong Wang,Fengbang Wang,Lei Bi,Chunyan Ma,Maoyong Song,Guibin Jiang
Bisphenol A (BPA) and its analogs are endocrine-disrupting chemicals that are frequently detected in environmental and human samples. However, the effective removal of BPA and its analogs has not yet been extensively studied. Herein, we introduce a novel enzyme reactor for the degradation of BPA and its analogs in water. The influence of pore size on the degradation efficiency of immobilized laccase in the spatial nanopores of hydrogel was investigated using BPA as a representative compound. This showed that nanopores enhance the activity of immobilized laccases in a pore size-dependent manner and increase their stability. Compared with the same amount of free laccase, the 50 mg/L BPA degradation performance of laccase immobilized in 76 nm nanopores increased to 300 %. Taking advantage of magnetic separation, this immobilized laccase can be reused, and its degradation capacity was maintained at over 73.7 % after ten reactions. Moreover, the degradation of seven BPA analogs was 1.03–5.88 times higher using laccase immobilized in nanopores compared with free laccase. Also, the biocatalyst could efficiently degrade BPA analogs in real water matrix. This study opens up a new avenue for the removal of BPA and its analogs by immobilizing laccase in nanopores, overcoming the key limitations introduced by the short enzyme life span and non-reusability.
研究领域:
环境科学
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生物技术
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酶学
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水处理工程
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DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2018
·Vol. 47
·期 12
·7514-7519
·
IF 1.566
Zhu Z.,Zhang Y. W.,Song H. Z.,Li X. J.
Cu 1.98− x Na x Se nanopowders were synthesized using a hydrothermal method and hot-pressed into bulk pellets to assess the effects of Na doping on the thermoelectric properties of Cu 1.98 Se. The original layered microstructure was maintained at low doping levels, although nanopores, together with point defects, appeared to decrease the thermal conductivity. The thermoelectric properties of the material were improved by Na doping, resulting in a maximum ZT value of 1.32 for Cu 1.94 Na 0.04 Se at 973 K. The experimental data indicate that an optimal level of Na doping effectively improves the thermoelectric performance of non-stoichiometric Cu 1.98 Se.
研究领域:
材料科学
·
热电学
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DOI
高影响因子
ACS Sensors
·
2019
·
IF 6.944
Liping Liu,Zhen Fang,Xiangjiang Zheng,Dongmei Xi
A straightforward yet efficient, nanopore-based strategy that enables the sensitive detection of copper(II) ion (Cu2+) and real-time monitoring of a click reaction is provided. Two single-stranded DNAs (ssDNAs) are designed to act as the preprobes, one being modified with an azide and the other an alkyne. The presence of Cu2+ induces the ligation of two ssDNAs via click reaction, leading to the formation of a forked DNA which can quantitatively generate characteristic current signatures when interacts with α-hemolysin (α-HL) nanopore. The assay facilitates a highly selective and sensitive measurement of Cu2+ without the need for labels or signal amplification. More importantly, this nanopore platform exhibits excellent performance in real-time monitoring of a copper(I) ion (Cu+)-catalyzed click reaction at the single-molecule level, by recording the current signals of the forked DNA generated by click chemistry. The proposed strategy is believed to play an important role in both nanopore sensing and characterization of chemistry reactions, especially coupling reactions.
研究领域:
纳米孔传感
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化学反应表征
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生物分析化学
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Libo Zhu,Zhen Zhang,Quanjun Liu
With the development in DNA self-assembly technology, DNA origami nanostructures have been widely applied in biomedical research. Solid-state nanopores represent an emerging single-molecule sensing platform for studying nanostructures with arbitrary dimensions and physical characteristics, including DNA origami. Here, we employed relatively narrow silicon nitride nanopores to detect the deformation and translocation of DNA origami nanoplates with dimensions of approximately 60 × 54 nm. We performed translocation experiments using three nanopore diameters that are all smaller than the plat dimensions. Analysis of current blockade signals and the representative events reveals three types of translocation orientations for the nanoplates. Furthermore, by studying the electrical signal characteristics (current change and dwell time) for the different diameter pores, we obtained information about the translocation behaviors for the DNA nanoplates through different constrictions. Our investigation provides an approach to analyze the deformation and translocation of DNA origami structures.
研究领域:
生物物理学
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纳米技术
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单分子检测
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DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Shilong Li,Kecheng Guan,Dandan Zhou,Dong Zou,Jian Lu,Wenbo Jiang,Bin Chen,Jian Qiu,Lele Cui,Tianxiang Yu,Yuqing Sun,Zhi Xu,Wanqin Jin,Wenheng Jing
Two-dimensional (2D) material membranes have significant potential for selectively transporting molecules and ions, crucial for environmental and energy applications significantly. However, challenges such as complex pathways and instability due to weak interactions hinder their performance. This study takes 2D MXene (Ti3C2Tx) as a platform and proposes a lattice vacancy-anchored chemical etching method to perforate MXene nanosheets and produce acid cross-linkers simultaneously. The etching process involves H2O2 oxidation consuming Ti atoms from the lattice vacancies of MXene to create peroxo titanic acid (PTA), precisely generating nanopores in the nanosheets for additional transport pathways. At the same time, the produced PTA acts as a cross-linking agent that enhances the interaction between MXene nanosheets to form stabilized interlayer channels. This approach results in multilevel features in the MXene membrane, offering abundant vertical water channels and robust interlayer ion-sieving channels. Consequently, both permeability and selectivity improve nearly 10-fold compared to the pristine membrane, overcoming previous trade-offs. This strategy presents a precise and ingenious method for perforating 2D materials and constructing high-performance mass transport channels of 2D membranes at various levels, benefiting sustainable and highly efficient desalination processes.
研究领域:
材料科学
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环境工程
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化学工程
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产品关联
DOI
PubMed
高影响因子
Matter
·
2025
·
IF 17.5
Kui Li,Tian Li,Kefan Wang,Yifan Wang,Zixuan Wang,Bingxiao Cheng,Yusheng Ouyang,Panke Zhang,Shuo Huang
Urine, an easily accessible body fluid, contains rich metabolic compounds reflecting the human health condition. Though urine analysis can be carried out by many conventional analytical methods, direct, rapid, and simultaneous identification of a variety of urinary metabolites by a handheld device remains challenging. Recent developments in nanopore small molecule sensing may suggest direct urine analysis performed by an engineered nanopore. Here, a hetero-octameric Mycobacterium smegmatis porin A (MspA) nanopore immobilized with a single nickel ion is confirmed to be suitable for the simultaneous identification of six urinary metabolites: creatinine, taurine, histidine, 1-methylhistidine, 3-methylhistidine, and hypoxanthine. With the assistance of machine learning, an overall accuracy of 98.5% was reported. This urine sensing capacity was further applied in real-time urinary metabolite monitoring after diet and physical exercise. These proofs of concept suggest a variety of nanopore-based body fluid analyses for early diagnosis, health management, pharmacokinetic monitoring, and doping tests.
研究领域:
分析化学
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纳米技术
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代谢组学
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生物医学工程
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机器学习
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运动科学
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营养科学
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产品关联
DOI
高影响因子
MACROMOLECULES
·
2022
·
IF 6.057
Jing He,Jianing Zhou,Jinxian Yang,Mo Zhu,Lianwei Li
Exploring an efficient way to purify the graft-onto polymerization products is an important but challenging problem. This work explores the feasibility of utilizing nanopore-based ultrafiltration for this purpose. First, a library of PPANb-g-PSNg-σ graft-onto products with polystyrene (PS) sidechain and poly(propargyl acrylate) (PPA) backbone has been prepared, and the effect of molecular parameters, including contour length (lc), sidechain radius (Rsidechain), and persistence length (lp), on the flow-assisted translocation and cutoff performance of PPANb-g-PSNg-σ through nanopores with diameter D = 20 nm has been studied. At a low macroscopic flow rate Q = 0.2 mL/h, it is found that for PPANb-g-PSNg-σ chains with Rsidechain > 1/2D, they are completely retained due to the size-sieving effect. More generally, it is found that the decrease of lc from ∼500 to ∼50 nm and Rsidechain from 18.0 to 3.9 nm facilitates the diffusive passage of chains through nanopores; surprisingly, the increase of lp from 5.4 to 22.2 nm also facilitates the process, which actually signifies the possibility of enhanced chain orientation. At a high macroscopic flow rate Q > 0.2 mL/h, the increase in lc and Rsidechain results in a larger critical macroscopic flow rate (Qc) for chains to squeeze into the nanopores. When Rsidechain > 1/2D, no lc-dependence is observed for the cutoff performance. Furthermore, it is found that the separation efficiency can be efficiently enhanced by increasing the solution concentration in the regime of C < C* with C* being the overlap concentration, which is demonstrated by one example of the practical purification process of the PPA780-g-PS41-60% model sample.
研究领域:
化学工程
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材料科学
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生物技术
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Mengya Cao,Hao Wang,Haoran Tang,Dandan Zhao,Yongxin Li
Metal–organic frameworks (MOFs) can improve the stability and activity of enzymes under the MOF encapsulation. However, it remains a challenge to explore the effects of the MOF environment on enzymatic activity in a confined space. In this work, we immobilized the enzyme inside a glass nanopore to study the catalytic activity and stability of the enzyme in the MOF environment. Horseradish peroxidase (HRP) is encapsulated in zeolitic imidazolate framework-90 (ZIF-90) and zeolitic imidazolate framework-8 (ZIF-8), which are used as the catalytic platforms. The HRP can catalyze 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) molecules to generate ABTS+ ions, and the change of the transmembrane ion current will be monitored in real time. As the concentration of H2O2 increases, the amount of produced ABTS+ will increase; thus, the ionic current increases. The effects of the MOF structure on enzyme activity and stability are also investigated. The HRP encapsulated in the MOF and modified inside the nanopore provides a novel and unlabeled design for studying enzymatic catalysis in a confined environment, which should have extensive applications in chemical-/bio-sensing, electrocatalysis, and fundamental electrochemistry.
研究领域:
生物化学
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材料科学
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分析化学
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DOI
PubMed
高影响因子
Matter
·
2024
·
IF 17.3
Pingping Fan,Kui Li,Tian Li,Panke Zhang,Shuo Huang
Alcoholic beverages, such as wine, beer, and distilled spirits, are widely produced and consumed in different nations. Different types of alcoholic beverages contain different combinations of flavor compounds. However, rapid and simultaneous analysis of a large variety of compounds in alcoholic beverages by a miniatured and portable device remains a challenge. In this paper, a Mycobacterium smegmatis porin A (MspA) nanopore modified with a phenylboronic acid (PBA) adapter is applied for rapid analysis of a variety of alcoholic beverages. By utilizing custom machine learning algorithms, various cis -diols are identified simultaneously in both distilled and fermented alcoholic beverages, generating unique barcodes for each sample type. Nanopore analysis of alcoholic beverages has also never been carried out previously. Rapid grading of wine sweetness and detection of additives, including sucrose and D-tartaric acid, are also demonstrated, showcasing the significance of this technique in the administration of wine production.
研究领域:
生物技术
·
食品科学
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产品关联
DOI
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Linhan He,Kaili Ling,Mingjie Wang,Xuejin Zhang,Jing Li,Yao Chen,Yongming Fan
This study highlights the significance of the difference between the melting and solidification temperatures of phase change materials (PCMs) in their practical applications. We incorporated the wood fiber as a support phase and used alkylation to modify the pore structure and surface properties of silica aerogels . The effect of different pore size distributions and surface properties on the phase change properties of PCMs was investigated. The results showed that nanopores increased pressure and the melting point of PCMs while the weak interaction force on the substrate surface decreased their solidification temperature. Furthermore, using matrices with unique properties, such as mesopore and hydrophobic aerogels , proved to be effective in reducing the difference between the melting and solidification temperatures by up to 44.1 % compared to pure paraffin wax. Moreover, hydrophobic groups enhanced the encapsulation performance of the composite, effectively isolating the influence of water.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 18
·10737-10747
·
IF 3.108
Jian Sui,Shi Qi Zhang,Min Zhai,Fang Tian,Jian Zhang,Xiao Zheng Lan
Inspired by the interest in the confinement effect on polymorphism of alkane mixtures, phase behaviors of a hexadecane–heptadecane (n-C16H34–C17H36, C16–C17) binary system in the bulk and in nanopores of controlled porous glasses (CPGs) are investigated using differential scanning calorimetry (DSC) and temperature-dependent powder X-ray diffraction (XRD). As reported, bulk C16–C17 mixtures form two intermediate phases with monoclinic (Mdci) and orthorhombic (Op) structures in a low temperature range and rotator phase RI in high temperature range. In the pores of CPG (300 nm), these solid phases appear in the temperatures slightly below the bulk system. The RI one-phase region extends a bit in temperature range in the mixtures of high C17 components. In CPG (8.1 nm), the solid phases come up in much lower temperatures than the bulk. The rotator RI is found in a larger range of the temperature and composition than the bulk and in CPG (300 nm). A new phase, the rotator RII, is observed in a narrow temperature range of high C17 composition just before melting. XRD analysis shows diffractions from the alkane layered ordering in CPG (300 nm) with a more roughened layer surface than the bulk. Lamellar stacking of the solids in CPG (8.1 nm) is heavily perturbed. The variation in the polymorphism of C16–C17 mixtures in the nanopores is associated with the reduced ordering of the molecule arrangements. These results can help to understand the influence of confinement effect on binary mixtures of alkanes, the chain molecules.
研究领域:
物理化学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2023
·
IF 3.3
Xinjia Zhang,Yanchao Li,Jianguo Mi,Xiuqing Kang,Junsu Jin
Atmospheric water adsorption at low humidity is considered to be a major challenge in solving global water problems. As a routine water adsorbent, the University of Oslo-66 (UiO-66) powder has been restricted by its limited low-pressure water absorption capacity, the need for powder molding, and insufficient adsorption rates. To overcome these problems, this work prepares E-UiO-66@PAA spherical composites by crystallizing UiO-66 on the surface of macroporous polyacrylate, i.e. PAA porous spheres, through in situ confined growth and ligand exchange strategies. The average size of the crystals has been reduced by 4 times vtihe nanoscale roughness of the polymer substrate surface and the interfacial enrichment of the precursor solution to lower the energy barriers of heterogeneous nucleation. In addition, the coordination of the hybrid ligand with Zr 4+ creates a unique cavity structure and gives rise to novel micropores as a result of ligand defects, which can be fitted into the lattice as binding sites for water. In the water vapor adsorption test at 298 K, E-UiO-66@PAA was able to operate at 4.9 times the capacity at low pressure (0.1 < P/P 0 < 0.2) and advanced the adsorption saturation time by 30.7% of the pristine UiO-66. Especially, the relative pressure of the sudden change in water vapor uptake was reduced from 0.3 to 0.1, which could greatly save the energy required for vapor regeneration and reduce its operating cost. This study provides an effective method to improve the adsorption capacity, mass transfer rate, and workability of composites by designing a nanoporous structure and confined growth nuclei as adsorption sites.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）