Energies
·
2023
·Vol. 16
·期 13
·4859
·
IF 3.2
Camilo Andrés Guerrero-Martin,Daniel Montes-Pinzon,Mariana Meneses Motta da Silva,Erik Montes-Paez,Laura Estefanía Guerrero-Martin,Raúl Salinas-Silva,Stefanny Camacho-Galindo,Elizabete Fernandes Lucas,Alexandre Szklo
Asphaltene precipitation/deposition is considered a problem of formation damage, which can reduce the oil recovery factor. In addition, asphaltenes can be deposited in pipelines and surface installations, causing serious complications in guaranteeing runoff, decreasing the production of oil wells. The precipitation of asphaltenes can be minimized by reducing the oil production flowrate or by using chemical inhibitors. Analyzing the stability and precipitation trend of asphaltenes in petroleum is vital for the guarantee of flow. For this purpose, several experimental and numerical methods have been proposed. Once the risk of precipitation is established, strategies can be formulated for the prevention and diagnosis of deposition problems in production or production training. The tests can be performed with dead oil, available in the wellhead, and help in understanding the behavior of the asphaltenes. This review aims to present (i) the problem related to the precipitation of asphaltenes; (ii) thermodynamic models of asphaltene precipitation; and (iii) asphaltene inhibition, control, and removal techniques using nanoparticles.Keywords:asphaltenes;formation damage;precipitation/deposition
研究领域:
石油工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
DENTAL MATERIALS
·
2019
·
IF 4.44
Manlin Qi,Xue Li,Xiaolin Sun,Chunyan Li,Franklin R. Tay,Michael D. Weir,Biao Dong,Yanmin Zhou,Lin Wang,Hockin H.K. Xu
Objective Periodontal tissue destruction and tooth loss are increasingly a worldwide problem as the population ages. Periodontitis is caused by bacterial infection and biofilm plaque buildup. Therefore, the objectives of this study were to: (1) develop a near-infrared light (NIR)-triggered core-shell nanostructure of upconversion nanoparticles and TiO 2 (UCNPs@TiO 2 ), and (2) investigate its inhibitory effects via antibacterial photodynamic therapy (aPDT) against periodontitis-related pathogens. Methods The core β-NaYF 4 :Yb 3+ ,Tm 3+ were synthesized via thermal decomposition and further modified with the TiO 2 shell via a hydrothermal method. The core-shell structure and the upconversion fluorescence-induced aPDT treatment via 980 nm laser were studied. Three periodontitis-related pathogens Streptococcus sanguinis ( S. sanguinis ), Porphyromonas gingivalis ( P. gingivalis ) and Fusobacterium nucleatum ( F. nucleatum ) were investigated. The killing activity against planktonic bacteria was detected by a time-kill assay. Single species 4-day biofilms on dentin were tested by live/dead staining, colony-forming units (CFU), and metabolic activity. Results The hexagonal shaped UCNPs@TiO 2 had an average diameter of 39.7 nm. UCNPs@TiO 2 nanoparticles had positively charged (+12.4 mV) surface and were biocompatible and non-cytotoxic. Under the excitation of NIR light (980 nm), the core NaYF 4 :Yb 3+ ,Tm 3+ UCNPs could emit intense ultraviolet (UV) light, which further triggered the aPDT function of the shell TiO 2 via energy transfer, thereby realizing the remarkable antibacterial effects against planktons and biofilms of periodontitis-associated pathogens. NIR-triggered UCNPs@TiO 2 achieved much greater reduction in biofilms than control (p < 0.05). Biofilm CFU was reduced by 3–4 orders of magnitude via NIR-triggered aPDT, which is significantly greater than that of negative control and commercial aPDT control groups. The killing efficacy of UCNPs@TiO 2 -based aPDT against the three species was ranked to be: S. sanguinis < F. nucleatum = P. gingivalis . Metabolic activities of biofilms were also greatly reduced via NIR-triggered aPDT (p < 0.05). Significance Upconversion fluorescence-based aPDT achieved strong inhibiting effects against all three species of periodontitis-related pathogens. This novel nanotechnology demonstrated a high promise to inhibit periodontitis, with exciting potential to combat other oral infectious diseases such as deep endodontic infections.
研究领域:
口腔医学
·
纳米生物学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Yue Chen,Xiaoqiang Yan,Wei Yang,Jing Wang,Qiaoyun Lu,Bingzhi Li,Wanying Zhu,Xuemin Zhou
The variation patterns of transcription factors (TFs) provide direct information for the states of cell populations, which is of significance for biomedical research and clinical diagnostics. Herein, we show that through multi-channeled isothermal amplification, it is feasible to connect DNA-based signal transduction with chromatography for multiplexed detection of TFs. The described system is referred to as "PAC" which includes three major steps: (i) P rotection, which uses DNA-modified magnetic beads to capture TFs and converts the capturing event into triggering signal; (ii) A mplification, which receives the triggering signal and generate DNA reporters through multi-channeled extension and nicking of oligonucleotides; and (iii) C hromatography, which separates and detects the DNA reporters in liquid chromatography. The quantitative detection of five essential TFs includes p50, p53, AP-1, MITF, and c-Myc is realized in a multiplexed manner, with the lowest detection limit of 0.5 pM. PAC can also provide effective means to measure the above five TFs in real samples, including cultured cells, xenograft tumors, and blood-based liquid biopsy. This study not only established a solution for multiplexed measurement of TFs for molecular diagnostics, but also paved avenue for bridging the gap between DNA nanotechnology and chromatography.
研究领域:
生物医学工程
·
分子诊断学
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纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Ruiqi Yan,Sen Lin,Weinan Jiang,Xia Yu,Lei Zhang,Wentao Zhao,Qian Sui
Magnetic nanotechnologies have been shown to be an efficient approach to the reduction of microplastic (MP) pollution in aquatic environments. However, uncertainties remain regarding the relationship between particle stability and MP removal under varying water conditions, hindering the practical application of magnetic nanotechnologies for MP removal. Herein, the influence of particle aggregation behavior on nano-scale MP removal by Fe 3 O 4 nanoparticles (FNPs) was investigated, by monitoring dynamic light scattering parameters and analyzing the microstructures of particle aggregates. Results showed that 83.1 %–92.9 % of MPs could be removed by FNPs within 1 h, and MP removal exhibited a high degree of Pearson correlation ( R = 0.95; P = 0.04) with particle aggregation behavior mediated by the FNPs dosage. Furthermore, pH-dependent electrostatic interactions significantly influenced particle aggregation behavior and the removal of MPs. Under pH <6.7 conditions, electrostatic attraction between electropositive FNPs and electronegative MPs led to charge neutralization-induced aggregation and efficient removal MP performance. Under increasingly saline conditions, compression of the electrical double layer enhanced the self-aggregation behavior of MPs, weakening the electrostatic repulsion between FNPs and MPs under alkaline conditions. Therefore, salinity improved the MP removal efficiency, especially under alkaline conditions, with MP removal increasing from 4.47 % to 55.1 % when the mass fraction of NaCl was increased from 0 % to 1 %. These findings further our understanding of the effect of aggregation behavior on MP removal by FNPs and highlight the potential for magnetic nanotechnology application in the removal of nano-scale MPs from aquatic environments, while also providing valuable insights for the design of FNP-based materials.
研究领域:
环境科学
·
纳米技术
·
水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Materials Interfaces
·
2021
·Vol. 9
·期 4
·2101727
·
IF 6.147
Weiye Zhang,Beibei Wang,Jingmeng Sun,Yanchen Li,Junqi Zhao,Yi Liu,Hongwu Guo
Next-generation flexible electronics must achieve multifunctionality, environmental friendliness and antibacterial activity. Accordingly, organisms in nature are interconnected. Inspired by the honeycomb multilayer porous structure, a wood-nanotechnology-derived flexible membrane circuit is created to meet the abovementioned requirements. The flexible wood (FW) matrix is made of natural balsa wood that underwent a simple top-down chemical treatment. The multiwalled carbon nanotube (MWCNT) acts as a “bridge” between the FW matrix with a porous array structure and the active material (silver nanoparticles (Ag NPs) and poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS). Due to the three-dimensional porous microstructure and highly conductive surface inherited by the wood-nanotechnology-derived flexible membrane (FW/MWCNT/Ag/PEDOT:PSS), it shows a high area capacitance (266.7 mF cm −2 at 20 mV s −1 ) and a good long-term cycling stability 84.3% capacitance retention after 5000 cycles at 5 mA cm −2 when used as a supercapacitor electrode. In addition, it shows an excellent specific electromagnetic shielding efficiency (up to 970 dB cm 2 g −1 ), proving its application potential in the field of electromagnetic shielding. Because of the biocidal property of Ag NPs, the FW/MWCNT/Ag/PEDOT:PSS shows a remarkable antibacterial effect on Escherichia coli and Staphylococcus aureus . This strategy provides a new opportunity for researchers to design biomass-based integrated electronic materials.
研究领域:
材料科学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 6
·1524-1532
·
IF 4
Shuailong Zhou,Jianhan Ye,Xiaohuan Zhao,Zihao Zhou,Yuanchen Dong,Qian Shi,Nannan Liu,Fen Wu
The combination of DNA nanotechnology and nanopore sensing technology has greatly promoted research on target molecule or ion detection. The large solid-state nanopores/nanochannels show better mechanical stability and reproducibility, but metal ion detection in the large nanopores with diameters of hundreds of nanometers or several micrometers is rarely reported. Hence, it is meaningful and urgent to develop a large nanopore-based sensing platform for the detection of metal ions. Herein, we employed a salicylic aldehyde-modified DNA network in conjunction with a glass nanopipette (GN) with a diameter of hundreds of nanometers as a sensing platform for the detection of target metal ions. Upon the addition of different receptors with the amino group, the salicylic aldehyde could in situ specifically recognize and bind with Zn2+ and Al3, forming Schiff base-metal ion complexes at the four vertices of one face per nanocube unit. The steric hindrance effect of multiple Schiff bases and metal ion complexes leads to the blockage of internal structure and decrease of ion current in the GN. Owing to this signal amplification strategy, the detection limit of the target metal ion reaches a level of fM in the GN with a diameter of about 300 nm. In the future, this functional nanopore sensing platform is expected to realize highly sensitive detection for more biological metal ions by choosing appropriate receptors.
研究领域:
生物化学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Biological Sciences
·
2019
·Vol. 15
·期 5
·999-1009
·
IF 4.067
Ma Shijing,Miao Hongtao,Luo Yuan,Sun Yingming,Tian Xiaoli,Wang Feng,You Chengcheng,Peng Shan,Tang Guiliang,Yang Cui,Sun Wenjie,Li Shuying,Mao Yanling,Xu Jieyu,Xiao Yu,Gong Yan,Quan Hong,Xie Conghua
With the advancement of nanotechnology, various nanocomposites have been applied in the diagnostics and treatment of cancer. We synthetized FePt nanoparticles which were assembled on the surface of graphene oxide (GO). These novel FePt/GO nanosheets simultaneously act as a chemotherapy drug and enhance radiosensitivity. In this study, transmission electron microscope, dynamic light scattering, X-ray photoelectron spectroscope and Fourier transform infrared spectroscopy were used to characterize surface morphology and chemical composition of FePt/GO nanosheets (NSs). Their cytotoxicity in various cancer and normal cells was evaluated by cell counting kit-8 assay, and their effects on radiosensitization were determined by colony formation assay. To explore the underlying mechanisms, we measured the intracellular reactive oxygen species levels and autophagy formation. Monodansylcadaverine-staining, Western Blotting and ultrastructure analysis were utilized to assess autophagy. The results demonstrated that FePt/GO NSs not only selectively suppressed the proliferation of cancer cells, but also increased their radiosensitization. Moreover, FePt/GO NSs induced autophagy, which might result in promoted sensibilization of radiotherapy. In conclusion, with good safety and efficacy, FePt/GO NSs are safe and effective to suppress proliferation, enhance radiosensitization and induce autophagy of human non-small cell lung cancer cells. They are potential for the treatment of lung cancer.
研究领域:
纳米医学
·
肿瘤学
·
材料科学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Yingzi Liu,Meina Li,Fan Zhang,Anwei Zhu,Guoyue Shi
A Au disk nanoelectrode down to 3 nm in radius was developed by a facile and reliable method and successfully applied for monitoring dopamine release from single living vesicles. A fine etched Au wire was coated with cathodic electrophoretic paint followed by polyimide, which retracted from the tip end during curing to expose the Au nanotip. By cyclic voltammetric scanning the above tip in 0.5 M KCl, the transformation of a core-shaped apex into a geometrically well-defined Au disk nanoelectrode with different dimensions can be controllably and reproducibly achieved. Scanning electron microscopy, transmission electron microscopy, and steady-state voltammetry were used to determine the size of nanoelectrodes. The results showed that the specific etching and insulation method not only avoids the use of toxic etching solution and the uncontrollable treatment to expose the tip but also makes possible the controllable and reproducible fabrication of Au disk nanoelectrode down to 3 nm in radius. The nanoelectrodes with well-demonstrated analytical performance were further applied for amperometrically monitoring dopamine release from single rat pheochromacytoma cells with high spatial resolution.
研究领域:
分析化学
·
神经生物学
·
纳米科技
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2023
·
IF 2.2
Junwei Liu,Meihong Zhang,Chuanbin Wu,Xin Pan,Zhengwei Huang
Erastin is the most classical small molecular ferroptosis inducer but its clinical application is hindered due to its poor solubility. Nanotechnology has been used to improve ferroptosis inducer erastin’s solubility. The recently reported erastin-incorporated nanosystems (e.g., nanoparticulate ferritin, poly(ethylene glycol) methyl ether-blockpoly(lactide-co-glycolide) (mPEG-PLGA) nanoparticle, exosome, and liposome) could to some degree elevate its solubility, but were still poor in loading capacity. To be specific, the maximum entrapment efficiency and drug loading in previous studies were approximately 36% and 3.7%, respectively. In order to improve drug loading capacity, we constructed a novel carrier based on Vitamin E d-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) and polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (Soluplus®) blended micelles. The erastin@TPGS/Soluplus® micelles were prepared by thin film hydration method, and formulation details including dosage of Soluplus®, the weight ratio of TPGS to Soluplus®, the weight ratio of erastin to TPGS/Soluplus® were optimized. The physicochemical properties of the erastin micelles were measured by DLS, TEM, FTIR, and TG-DSC analysis. The products exhibited a nanoscale and uniform particle size (78.11 ± 0.54 nm) and neutral surface charge (-0.43 ± 0.32 mV). Significantly improved entrapment efficiency (89.63 ± 1.81%) and drug loading rate (10.03 ± 0.14%) were obtained, which was over 2-fold of current reports. The in vitro release study demonstrated that erastin could release in a sustained yet complete manner, and the drug release might be governed by multiple mechanisms including anomalous diffusion of non-Fickian diffusion, matrix erosion and sustained release. In summary, this proposed erastin nanosystem provides a new strategy for improving loading capacity, and may probably augment ferroptosis-inducing therapy for cancer treatment.
研究领域:
药学
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
AAPS PHARMSCITECH
·
2022
·Vol. 24
·期 1
·1-13
·
IF 4.026
Wang Guoliang,Li Yanchao,Qin Zhiguo,Liu Tao
Nanotechnology and coamorphous are both advanced technologies that can effectively improve the solubility of drugs. This study has been the first attempt to combine these two approaches to construct the coamorphous nanoparticles to improve the dissolution and investigated the effect of physical properties of coamorphous solid on the nanosizing process. Two types of coamorphous solid, i.e., curcumin-artemisinin and quercetin-lysine, were selected as models. Coamorphous curcumin-artemisinin could highly contribute to the size reduction during milling compared to the crystalline form, which might attribute to the change of crystallinity. Nanosized coamorphous curcumin-artemisinin showed higher dissolution than nanocrystals and single coamorphous sample. However, quercetin-lysine coamorphous nanoparticles did not reflect significant dissolution improvement compared with the microsized sample. The difference of initial dissolutions for both could be the main reason. The directly mixing and drying method was confirmed to be an effective and simple approach to maintain the dissolution of nanosized coamorphous sample.
研究领域:
药剂学
·
纳米医学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2017
·Vol. 82
·期 12
·2901-2909
·
IF 1.815
Ruixue Guo,Xinbo Guo,Xiaodan Hu,Arshad Mehmood Abbasi,Lin Zhou,Tong Li,Xiong Fu,Rui Hai Liu
The purpose of this work was to improve the oral bioavailability of a poorly soluble functional food ingredient, the total flavones of Hippophaë rhamnoides L. (TFH). A self-microemulsion drug delivery system (SMEDDS) was developed to overcome the problems of poor absorption of TFH in vivo . The optimal SMEDDS significantly enhanced the solubility of TFH up to 530 times compared to that in water. The mean droplet size was 61.76 nm with uniform distribution. And the loaded system was stable at 25 °C for 3 mo with transparent appearance. The in vitro release of TFH from SMEDDS was faster and more complete than that from suspension. After oral administration of TFH–SMEDDS in rats, the relative bioavailability of TFH was dramatically improved for 3.09 times compared with the unencapsulated form. The investigation indicated the potential application of SMEDDS as a vehicle to improve the oral bioavailability of TFH. Practical Application The lipid-based nanotechnology, namely self-microemulsion drug delivery system (SMEDDS) was used to improve the bioavailability and oral delivery of total flavones of Hippophaë rhamnoides L. (TFH). The relevant bioavailability of TFH could be remarkably 3-fold improved by the optimized SMEDDS. The SMEDDS produced via a simple one-step process for poorly soluble TFH to achieve a significant improvement in the bioavailability, may endorse the promising utilization of TFH in functional foods as well as pharmaceutical fields with an enhanced absorption in vivo .
研究领域:
药剂学
·
功能性食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Xiangyang Li,Yushuang Wei,Wenwen Li,Rong Xu,Cheng Xu,Beijing Luo,Kai Yang,Bing Yuan
The cryopreservation of human red blood cells (RBCs) is essential for maintaining a stable blood supply, but current glycerol-based methods are hindered by complex and time-consuming post-thaw processing. While trehalose is a promising biocompatible cryoprotectant, its efficient intracellular delivery into non-endocytic RBCs remains a major hurdle. Here, a lyophilized, ionizable lipid nanoparticle (LNP) platform is reported that overcomes this challenge via an acidic pH-triggered membrane fusion mechanism. This approach, inspired by mRNA vaccine technology, enables rapid (<30 min) and efficient intracellular delivery of trehalose. Systematic optimization of the LNP composition and process parameters resulted in a remarkable post-thaw RBC recovery rate of 85%, an efficacy comparable to the clinical glycerol standard but achieved with a simple, single-wash protocol. The efficacy depends on the specific lipid synergy to enable pH-responsive fusion. Critically, the lyophilized LNP powder retains full functionality, ensuring long-term stability and simplifying storage and transportation logistics. This study establishes a clinically relevant, translation-ready cryopreservation strategy that bridges nanotechnology and transfusion medicine, offering a next-generation solution to longstanding challenges in cell preservation.
研究领域:
纳米技术
·
生物医学工程
·
输血医学
·
低温生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）