Biomolecules
·
2020
·Vol. 10
·期 4
·575
·
IF 4.082
Xin Wang,Xuan Wu,Zhisong Lu,Xiaoqi Tao
Label selection is an essential procedure for improving the sensitivity of fluorescence immunochromatography assays (FICAs). Under optimum conditions, time-resolved fluorescent nanobeads (TRFN), quantum dots nanobeads (QB) and quantum dots (QD)-based immunochromatography assays (TRFN-FICA, QB-FICA and QD-FICA) were systematically and comprehensively compared for the quantitative detection of aflatoxin B1(AFB1) in six grains (corn, soybeans, sorghum, wheat, rice and oat). All three FICAs can be applied as rapid, cost-effective and convenient qualitative tools for onsite screening of AFB1; TRFN-FICA exhibits the best performance with the least immune reagent consumption, shortest immunoassay duration and lowest limit of detection (LOD). The LODs for TRFN-FICA, QB-FICA and QD-FICA are 0.04, 0.30 and 0.80 μg kg−1in six grains, respectively. Recoveries range from 83.64% to 125.61% at fortified concentrations of LOD, 2LOD and 4LOD, with the coefficient of variation less than 10.0%. Analysis of 60 field grain samples by three FICAs is in accordance with that of LC-MS/MS, and TRFN-FICA obtained the best fit. In conclusion, TRFN-FICA is more suitable for quantitative detection of AFB1in grains when the above factors are taken into consideration.Keywords:time-resolved fluorescent nanobeads;quantum dot;quantum dot nanobeads;immunochromatography;aflatoxin B1
研究领域:
分析化学
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食品科学
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毒理学
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生物技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Chunjing Feng,Zhun Hou,Wei Jiang,Lihong Sang,Lei Wang
Herein, a sensitive and enzyme-free assay for adenosine detection has been developed on the basis of binding induced colocalization activated hybridization chain reaction (HCR) strategy on the surface of magnetic nanobead . First, the recognition probe was fabricated and divided into two parts: the Apt-1 that composed a part of adenosine aptamer and toehold domain, and the Apt-2 that consisted of another part of adenosine aptamer and branch migration domain. The Apt-1 was immobilized on a streptavidin-magnetic nanobead (streptavidin-MNBs) that played the roles of enrichment and separation. Then the recognition event of adenosine could bring the two parts of aptamer together and induce the colocalization of toehold domain and branch migration domain, which could serve as an integrated initiator to trigger the HCR, producing a long nicked double-stranded polymer. Finally, the intercalating dye SYBR Green I was inserted into the polymer, generating an enhanced fluorescence signal. In this strategy, the initiator was divided into two parts and could be suppressed effectively in the absence of adenosine. Utilizing the separated function, the spontaneous hybridization of H1 and H2 could be avoided, and a low background could be acquired. Moreover, through the double amplification of HCR and multimolecules binding of SYBR Green I, highly sensitive and enzyme-free detection were achieved. The detection limit for adenosine detection was 2.0×10 −7 mol/L, which was comparable or superior to the previous aptasensors . Importantly, adenosine analysis in human urines has been performed, and this strategy could significantly distinguish the adenosine content in normal human urines and cancer patient urines, suggesting that this proposed assay will become a reliable and sensitive adenosine detection method in early clinical diagnosis and medical research.
研究领域:
生物化学
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医学诊断
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Xiaolun Peng,Huangli Yan,Zhen Wu,Wei Wen,Xiuhua Zhang,Shengfu Wang
Detection of biomarkers at the cellular level can provide more accurate and comprehensive information that is important for early diagnosis of diseases and evaluation of new drugs. However, the interference of a large number of components in cells and the requirement of high sensitivity bring great challenges for their detection. Herein, a robust and enzyme-free electrochemical platform was proposed for microRNA-21 (miRNA-21) detection by integrating the efficient separation of magnetic nanobeads (MBs) with the multisignal amplification of strand displacement amplification (SDA) and electrochemically mediated atom transfer radical polymerization (eATRP). The eATRP is capable of de novo growth of a number of electroactive polymers on the electrode surface for signal amplification. Compared to simple hybridization, SDA and eATRP can enhance the signals by ∼35-fold, achieving high signal-to-noise ratio for low-abundant target detection. Owing to their superparamagnetism and strong magnetic response ability, MBs endow the method with excellent specificity and anti-interference ability to detect miRNA-21 in cells. Using MBs as capture carriers, SDA and eATRP for signal amplification, and gold nanoflower (AuNF)-modified electrodes as working electrodes, as low as 0.32 aM miRNA-21 was detected. Furthermore, the successful detection of miRNA-21 in cells indicated the great prospect of this method in the early diagnosis of cancers, life science research, and single-entity electrochemical detection.
研究领域:
生物医学工程
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分子诊断学
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电化学传感器
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DOI
高影响因子
SENSORS
·
2021
·Vol. 21
·期 19
·6604
·
IF 3.576
Daixian Wei,Jintao Liu,Zexiang Wang,Shu Zhou,Suhua Wang,Weipeng Tong,Juan Peng
Sulfamethazine (SMZ) as a broad antibiotic is widely used in livestock and poultry. However, the abuse of SMZ in livestock feed can lead to SMZ residues in food and the resistance of bacteria to drugs. Thus, a method for the detection of SMZ in food is urgently needed. In this study, quantum dot (QD) nanobeads (QBs) were synthesized by encapsulating CdSe/ZnS QDs using a microemulsion technique. The prepared QBs as signal probes were applied in lateral flow immunoassay (LFIA) for the detection of SMZ in chicken and milk. Our proposed method had limits of detection of 0.1138–0.0955 ng/mL and corresponding linear ranges of 0.2–12.5, 0.1–15 ng/mL in chicken and milk samples, respectively. The recovery of LFIA for the detection of SMZ was 80.9–109.4% and 84–101.6% in chicken and milk samples, respectively. Overall, the developed QBs-LFIA had high reliability and excellent potential for rapid and sensitive screening of SMZ in food.Keywords:lateral flow immunoassay (LFIA);quantum dot nanobeads (QBs);class-specific monoclonal antibody;sulfamethazine (SMZ);chicken
研究领域:
食品安全检测
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生物技术
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
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2016
·Vol. 6
·期 90
·86829-86835
·
IF 3.289
Ling Chang,Shuquan Chang,Wei Han,Wei Chen,Zheng Li,Zheng Zhang,Yaodong Dai,Da Chen
Novel porous permutite/carbon nanobeads/amidated alginic acid nanocomposites were successfully fabricated via a gamma radiation method. The carbon nanobeads are 40–60 nm in diameter. The cross-linked amidated alginic acid is well dispersed on the surface of carbon nanobeads and permutite. The preparation parameters such as reactant ratio, carbonization conditions and radiation dose were optimized. The influences of contact time, pH and initial concentration of the Cs+ solution were investigated. Contact time = 4 h and pH = 9 are ideal conditions for Cs+ removal. The Cs+ removal efficiency and adsorption capacity can reach 98% and 60.1 mg g−1 respectively. They have good stability and adsorption–desorption properties. The adsorption capacity can maintain more than 85% after 4 adsorption–desorption cycles. The preparation strategy and adsorption mechanism were also discussed. Gamma radiation technology exhibits distinguished advantages to improve the dispersion and stability of organic/inorganic hybrid materials. This method can be easily applied to prepare other similar porous hybrid adsorbents.
研究领域:
材料科学
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环境科学
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核化学
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产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 67
·41989-41999
·
IF 3.108
Dan Su,Hanyun Li,Jinlin Li,Yali Liu,Mi Peng,Bingwei Feng,Pengfei Xu,Yonggui Song
This work reports on a simple, feasible and optical color-enhanced colorimetric immunoassay with hemin (a horseradish peroxidase mimic enzyme) catalyzed color reaction and multifunctional colored silica nanoparticles for qualitative and quantitative determination of avian influenza A (H7N9) virus (H7N9 AIV) at an ultralow concentration by using the magnetic nanobeads (MBs)-mimic enzyme-multifunctional silica nanoparticles color-enhanced chromogenic substrate system. In this work, MBs were fabricated by co-immobilizing target recognition molecules (mAb–MB), which were used as both separation and enrichment carriers. The multifunctional colored silica nanoparticles were synthesized by doping Cy2 (red organic dye) into silica nanoparticles using an inverse microemulsion method, and then made a covalent modification of pAb and glucose oxidase (GOx). In the presence of H7N9 AIV, GOx catalyzed glucose to gluconic acid and hydrogen peroxide (H2O2). The latter can oxidize 4-aminoantipyrine (4-AAP) to red products and the reaction is catalyzed by hemin. The chromogenic compound and the red-silica nanoparticles (Red-SiNPs) together cause the color of the solution to change from colorless to red. Through this color-enhanced immunoassay, we can detect H7N9 AIV with the naked eye and UV detector. And the immunosensor provides a simple and reliable platform with high sensitivity and selectivity which shows great potential in early diagnosis of diseases and in public health security.
研究领域:
医学诊断
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生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
·
2024
·Vol. 15
·期 1
·10
·
IF 4.9
Yutong Wu,Yang Liu,Jinchen Ma,Shanxi Zhu,Xiaojun Zhao,Huawei Mou,Xuanqi Ke,Zhisheng Wu,Yifei Wang,Sheng Lin,Wuzhen Qi
Microbial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and sensitive detection of Salmonella in traditional Chinese medicine on a microfluidic chip. Immune gold@platinum nanocatalysts (Au@PtNCs) were utilized for specific bacterial labeling, while magnetic nano-beads (MNBs) with a novel high-gradient magnetic field were employed for the specific capture of bacteria. The immune MNBs, immune Au@PtNCs, and bacterial samples were introduced into a novel passive microfluidic micromixer for full mixing, resulting in the formation of a double-antibody sandwich structure due to antigen–antibody immune reactions. Subsequently, the mixture flowed into the reaction cell, where the MNBs- Salmonella -Au@PtNCs complex was captured by the magnetic field. After washing, hydrogen peroxide-tetramethylbenzidine substrate (H 2 O 2 -TMB) was added, reacting with the Au@PtNCs peroxidase to produce a blue reaction product. This entire process was automated using a portable device, and Salmonella concentration was analyzed via a phone application. This simple biosensor has good specificity with a detection range of 9 × 10 1 –9 × 10 5 CFU/mL and can detect Salmonella concentrations as low as 90 CFU/mL within 74 min. The average recoveries of the spiked samples ranged from 76.8% to 109.5%
研究领域:
微生物学
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药物分析
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生物传感技术
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DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Jiaping Guo,Yameng Wang,Shanshan Niu,Hongping Li,Yongmei Tian,Songcheng Yu,Fei Yu,Yongjun Wu,Li-e Liu
The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination of human IgG and then expand it to analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM and IgG, which is of great significance for the screening and diagnosis of COVID-19. In this study, the magnetic Fe3O4 nanospheres coupled with mouse antihuman IgG (Ab1IgG) were used as an immune capture probe (Fe3O4@Ab1IgG) to capture and separate the target, and rabbit antihuman IgG (Ab2IgG) coupled with highly luminescent quantum dot nanobeads (QBs) as a fluorescence detection probe (QBs@Ab2IgG) was used to realize high sensitivity detection. After the formation of a sandwich immunocomplex, the fluorescence intensity of the precipitate after magnetic separation was measured at the excitation wavelength of 370 nm. Under optimal conditions, a wide linear range varying from 0.005 to 40 ng·mL–1 was obtained for the detection of human IgG with a lower limit of detection at 4 pg·mL–1 (S/N = 3). The recoveries of intra- and interassays were 90.0–101.9 and 96.0–106.6%, respectively, and the relative standard deviations were 6.3–10.2 and 2.6–10.5%, respectively. Furthermore, the proposed method was successfully demonstrated to detect human IgG in serum samples, and the detection results were not statistically different (P > 0.05) from commercial enzyme-linked immunosorbent assay kits. This method is sensitive, fast, and accurate, which could be expanded to detect the specific IgM and IgG antibodies against SARS-CoV-2.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
免疫学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Runxian Li,Decheng Suo,Zhengjie Yu,Zhiming Xiao,Peiling Wei,Shi Wang,Yuchao Feng,Xia Fan
The abuse of illegal β2-adrenergic agonists in animal feeding poses a threat to human health. Fluorescent lateral flow immunoassay (LFIA) is a promising point-of-care testing (POCT) method for monitoring illicit drug use on farms. Here, a traffic light- dual-color quantum dot nanobeads based fluorescent lateral flow immunoassay (QBs-LFIA) was developed for simultaneously detecting ractopamine (RAC) and clenbuterol (CLE). CdSe/ZnS quantum dot nanobeads that emit red and green fluorescence were introduced in this study. They were conjugated with anti-RAC and anti-CLE monoclonal antibodies, respectively, to prepare traffic light fluorescent probes (QBs-mAbs). The dual-color QBs-mAbs greatly enhanced the analytical performance and solved the restriction that multiple mAbs on the control line cannot be accurately distinguished in traditional single-color LFIA. Under optimal conditions, the limits of detection (LODs) for RAC and CLE were 15.7 and 36.3 pg/mL, respectively. The recoveries in spiked cattle urine ranged from 80.3 to 106.1 % with relative standard deviations (RSDs) less than 13.2 %. Good correlations were observed between the detection results of QBs-LFIA and LC-MS/MS (R 2 = 0.9931). The proposed traffic light QBs-LFIA provides a novel approach for simultaneously detecting RAC and CLE. It is also a promising POCT platform for rapid, sensitive, and on-site detection of multiple drug residues.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Xiaoyan Li,Yang Qi,Changxu Zhao,Fei Wang,Yanlei Ding,Guangzheng Hou,Pengcheng Cao,Zhe Zhou,Qiqi Liu
Sensitivity and accuracy are the key performance metrics for evaluating immunochromatographic assay (ICA). In this study, we have developed a quantum dot nanobeads-based dual-channel fluorescent ICA (QBs-DICA) designed for the sensitive and accurate detection of both influenza A virus (IAV) and human adenovirus (ADV) antigens. Following systematic assay optimization, we achieved a remarkable improvement in sensitivity, with gains of up to two orders of magnitude at the best. To enhance signal accuracy for IAV and ADV testing, we implemented a temperature-controlled dual-channel detector during sample incubation and the chromatographic process, resulting in an approximate increase of 34.2% and 36.7%, respectively. The Limit of Detection (LOD) for IAV and ADV testing was robustly established at 165 copies/mL and 805 copies/mL, respectively. Furthermore, in the case of IAV-spiked and ADV-spiked samples, we achieved consistently high recoveries ranging from 94.5% to 104.8% and 94.7% to 106.4%, with impressively low coefficients of variation (CV) of below 7.16% and 6.25%, respectively. Our established QBs-DICA system has exhibited a remarkable enhancement in both sensitivity and accuracy, making it exceptionally well-suited for supporting the rapid advancement of clinical diagnosis and treatment strategies.
研究领域:
医学诊断
·
病毒学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Theranostics
·
2018
·
IF 8.537
Lixin Hong,Kan Wang,Wenqiang Yan,Hao Xu,Qinghui Chen,Yuhui Zhang,Daxiang Cui,Qinghui Jin,Jinghua He
The detection of cardiac markers is critical to the diagnosis of acute myocardial infarction, and immunochromatographic assays are a common tool for point-of-care analysis. Methods : We report a multiplexed lateral flow test strip for simultaneous quantitative detection of cardiac troponin I (cTnI), creatine kinase isoenzyme MB (CKMB), and myoglobin (Myo). Hydrophilic, monodisperse, stable, and carboxyl-modified (COOH-) magnetic nanobeads (MNBs) were used to construct immunomagnetic probes specific to the three cardiac markers. The detection area of the sandwich-style complexes contained three test lines for cTnI, CKMB, and Myo. The magnetic signal intensity of the detection area in the nitrocellulose membrane was measured via a magnetic immunochromatography reader developed in house. Results : To optimize the assay, a modified working buffer was also investigated to improve the detection sensitivity, decrease the background noise, and shorten the detection time. The MNB-based immunochromatography test (MICT) strip offers a wide linear dynamic detection range, rapid detection, high sensitivity, and specificity. The limit of detection was 0.0089 ng/mL for cTnI, 0.063 ng/mL for CKMB, and 0.05 ng/mL for Myo with minimal cross-reactivity. There were 110 clinical human serum samples that were used to evaluate this platform with high correlation. Conclusion : MICT shows great potential as a supplemental method for in vitro diagnostics in the laboratory or in other point-of-care testing (POCT) applications.
研究领域:
医学诊断
·
纳米技术
·
生物医学工程
·
临床化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2020
·
IF 4.258
Silu Hou,Jingjiao Ma,Yuqiang Cheng,Hengan Wang,Jianhe Sun,Yaxian Yan
The mycotoxins in grains is a serious threat to the health of humans and animals. In this study, we demonstrated an approach for development of a fast and sensitive fluorescent immunochromatographic assay for fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and zearalenone (ZEN) detection using quantum dots nanobeads (QDNBs, 100 nm). Amplification of the fluorescence signal of the quantum dot by nanosphere coating can significantly improve the sensitivity of immunochromatographic test strip. The detection probes (FB1-McAb@QDNBs, DON-McAb@QDNBs, and ZEN-McAb@QDNBs) were prepared using the QDNBs with 615 nm emissions coupling with three monoclonal antibodies (McAb) against FB 1 , DON, and ZEN, respectively, in a competitive immunochromatography platform. The surface of the QDNBs is modified with a carboxyl functional group, which facilitates the coupling of the antibody and significantly improves the sensitivity of test strip. Compared with traditional ELISA , the immunoassay based on the McAb@QDNBs is more sensitive, with IC 50 of 12.66 ng/mL (FB 1 ), 2.97 ng/mL (DON), and 0.87 ng/mL (ZEN), of which the linear range were from 2.295 ng/mL to 69.867 ng/mL (FB 1 ), 0.465 ng/mL to 16.19 ng/mL (DON), and 0.295 ng/mL to 2.575 ng/mL (ZEN), respectively. The accuracy, repeatability, and specificity of the established test strip was evaluated by testing the spiked and natural samples. Furthermore, a high agreement was observed between the developed test strips and LC-MS/MS in simultaneously and quantitative detection of three mycotoxins. In brief, the developed approach was feasible and could be employed for simultaneously and quantitative mycotoxins detection in agricultural products.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）