TALANTA
·
2021
·
IF 6.057
Shemsu Ligani Fereja,Ping Li,Jinhan Guo,Zhongying Fang,Ziwei Zhang,Zhihua Zhuang,Xiaohui Zhang,Kaifan Liu,Wei Chen
Folic acid (FA) is the natural form of water-soluble vitamins widely found in most plants and animal products and its deficiency leads to several human body abnormalities. The advancements of metal nanoclusters are highly increasing due to their molecule-like optical properties and attractive applications. Because of increasingly demand of noble metal nanoclusters as sensing templates, different synthesis methods have been developed for facile synthesis of noble metal nanoclusters . Herein, red-emitting fluorescent bovine serum albumin (BSA)-capped Au–Ag bimetallic NCs are facilely synthesized through green one-pot synthetic approach. The effect of silver on the fluorescence properties of Au NCs was investigated and it was found that introduction of silver can enhance the fluorescence intensity . The fluorescence intensity of the as-prepared Au–Ag nanoclusters gets quenched in the presence of folic acid in an aqueous medium and it was used as ultrasensitive sensing probe for FA detection. The developed Au–Ag NCs-based sensing probe shows linear response in the wide range of 0–100 μM and the detection limit is as low as 0.47 nM. Its applicability has also been confirmed successfully in real human serum, urine and FA tablet samples. Due to the high stability, sensitivity and selectivity , the developed bimetallic cluster sensing system is highly promising to be applied in the pharmaceutical and clinical laboratories.
研究领域:
生物化学
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分析化学
·
医学诊断
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Mao Anqi,Wei Chunying
DNA-templated silver nanoclusters (DNA-Ag NCs) with cytosine (C)-rich sequences (four or more segments of consecutive (2–5) C-bases) were synthesized. They display green and/or orange/red emissions under different excitation wavelengths. There is indication that more consecutive C-bases lead to longer emission wavelengths. The ratio of the red and green emissions of the DNA-Ag NCs depends on whether the NCs were synthesized under acidic or basic conditions. We also prepared the DNA copper/silver nanoclusters (DNA-Cu/Ag NCs) which can be synthesized in shorter time and display higher stability. The DNA-Cu/Ag NCs, under 470 nm photoexcitation, always display green emission, with a peak at 550 nm, irrespective of whether being prepared under acidic or basic conditions. The fluorescence of the Cu/Ag NCs is selectively quenched by Hg(II) ions which can be quantified by this nanoprobe with a detection limit as low as 2.4 nM. The quenching mechanism was studied by Stern-Volmer plots and lifetime studies which revealed that both static and dynamic quenching are operative.
Schematic presentation of fluorescent DNA-Ag nanoclusters (NCs) exhibiting red and green emission under different pH values, and green emissive DNA-Cu/Ag NCs for sensitive and rapid detection of Hg 2+ .
研究领域:
生物化学
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分析化学
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纳米科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Hong Huang,Hua Li,Jiu-Ju Feng,Ai-Jun Wang
Herein, we demonstrate a facile, straightforward and green method for synthesis of highly red-emitting fluorescent bimetallic Au/Ag nanoclusters (NCs) employing lipoic acid as both a reducing agent and a protecting agent. The as-prepared Au/Ag NCs exhibited bright red emission with a strong peak centered at 630 nm (quantum yield measured as 6.4%), and showed excellent stability and remarkable temperature-dependent fluorescent property. The fluorescence responses were reversible and linear to the environment temperature from 20 to 65 °C with a resolution as high as 1.34 °C, which allows its potential application as a fluorescent nanothermometer. Moreover, Au/Ag NCs were also explored for selective and sensitive determination of Fe 3+ based on the specific quenching of the fluorescence by Fe 3+ , and explored for fluorescence imaging Fe 3+ in living HeLa cells thanks to their very low cytotoxicity.
研究领域:
材料科学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Menglei Sun,Yidan Liang,Shiyao Sun,Haifeng Ji,Xudong Yang
In the last decade, nanoclusters and carbon dots have been a promising class of nanomaterials with a wide range of potential applications in different industrial fields, through their favorable fluorescence properties for a variety of applications, especially in temperature detection and metal ion detection. In this study, sensitive detection of Hg 2+ and temperature was achieved by metal nanoclusters and carbon dots. Well-stabilized red fluorescent gold nanoclusters (AuNCs) were successfully prepared using a one-pot hydrothermal method, and Hg 2+ ions strongly quenched the fluorescence emission of AuNCs. Subsequently, we used this finding to develop a fluorescent nanoprobe for Hg 2+ detection, in which the fluorescence color of AuNCs transitions from red to blue as the concentration of Hg 2+ increases, thus providing a promising avenue for the detection of Hg 2+ in real environmental water samples. Simultaneously, CDs/AuNCs fluorescent nanocomposites are synthesized using a dehydration condensation process between CDs and AuNCs. It is noteworthy that the red fluorescence emission is significantly sensitive to temperature changes. With the gradual increase in temperature, the fluorescence color of CDs/AuNCs nanocomposites gradually changes from pink-purple to blue, making it a promising nanothermometer. In summary, we prepared a novel fluorescent probe for Hg 2+ and temperature detection.
研究领域:
纳米技术
·
环境监测
·
分析化学
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材料科学
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荧光光谱学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Physical Chemistry C
·
2017
·
IF 4.536
Jinsong Chai,Ying Lv,Sha Yang,Yongbo Song,Xiaofeng Zan,Qinzhen Li,Haizhu Yu,Mingzai Wu,Manzhou Zhu
In this work, we report the synthesis and crystal structure of Au38–xCux(2,4-DMBT)24 (x = 0–6, 2,4-DMBTH = 2,4-dimethylbenzenethiol) alloy nanocluster for the first time. A variety of characterizations including ESI-MS, TGA, and XPS reveal the composition as Au38–xCux(2,4-DMBT)24 (x = 0–6). The single crystal structure has been determined by an X-ray single crystal diffractometer. From the anatomy of the structure, a bi-icosahedral Au23 core is protected by six dimeric [−SR–M–SR–M–SR−] units (M = Cu/Au) and three monomeric [−SR–Au–SR−] units. It is interesting that all the Cu atoms are selectively doped in the motifs of the Au38–xCux(2,4-DMBT)24 nanocluster. This phenomenon is distinct from the exclusive core doping of the Ag atoms in the previously reported Au38–xAgx alloy. Both the experimental results and DFT calculations of UV–vis spectra imply that the optical property of the Au38–xCux(2,4-DMBT)24 nanocluster is consistent with that of the Au38(2,4-DMBT)24 nanocluster, because the Cu dopants make little contribution to the frontier orbitals of the alloy NC.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
Nano Research
·
2021
·Vol. 15
·期 4
·3065-3072
·
IF 8.897
Du Peng,Huang Kai,Fan Xiaoyuan,Ma Jingteng,Hussain Naveed,Wang Ruyue,Deng Bohan,Ge Binghui,Tang Haolin,Zhang Ru,Lei Ming,Wu Hui
Being a typical state of the art heterogeneous catalyst, supported noble metal catalyst often demonstrates enhanced catalytic properties. However, a facile synthetic method for realizing large-scale and low-cost supported noble metal catalyst is strictly indispensable. To this end, by making use of the strong metal-support interaction (SMSI) and mechanochemical reaction, we introduce an efficient synthetic route to obtain ultrafine Pt and Ir nanoclusters immobilized on diverse substrates by wet chemical milling. We further demonstrate the scaling-up effect of our approach by large-scale ball-milling production of Pt nanoclusters immobilized on TiO 2 substrate. The synthesized Pt/Ir@Co 3 O 4 catalysts exhibit superior oxygen evolution reaction (OER) performance with only 230 and 290 mV overpotential to achieve current density of 10 and 100 mA·cm −2 , beating the catalytic performance of Co 3 O 4 supported Pt or Ir clusters and commercial Ir/C. It is envisioned that the present work strategically directs facile ways for fabricating supported noble metal heterogeneous catalysts.
研究领域:
材料科学
·
催化化学
✓ 已验证
DOI
高影响因子
DALTON TRANSACTIONS
·
2020
·Vol. 50
·期 2
·562-567
·
IF 4.174
Lubing Qin,Feng Zhang,Xiaoshuang Ma,Yun Tang,Guanyu Ma,Zhenghua Tang
To advance the development of atomically precise Ag and Ag-alloyed nanoclusters, it is critical to develop effective synthetic methods. Herein, we successfully extend the CTAB (cetyl trimethyl ammonium bromide) reverse micelle method to synthesize a high-purity Ag44(p-MBA)30 (p-MBA = para-mercaptobenzoic acid) nanocluster and its corresponding alloy cluster Au12Ag32(p-MBA)30 in a short time (15 min and 5 min), with a high yield of ∼83% and ∼85%, respectively. Furthermore, the mechanism regarding the reverse micelle method has been clearly elucidated. Through characterizing the reaction system by Raman spectroscopy and NMR spectroscopy techniques, it can be revealed that employing CTAB to form reverse micelles to construct a sealed chemical environment is critical for realizing the fast and high-yield synthesis.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yu Tao,Ke Yi,Haixia Wang,Kai Li,Mingqiang Li
Since hepatitis B virus (HBV) infection can cause chronic viral hepatitis, increase the risk of liver fibrosis and cirrhosis, and ultimately result in hepatoma, it is of great significance to explore convenient, rapid, and sensitive diagnostic strategies for HBV DNA detection. In this study, we developed a novel and sensitive fluorescent biosensor for HBV detection based on the sensing system consisting of CRISPR-Cas12a enzymes and metal nanoclusters as the luminescent nanoprobes, involving gold nanoclusters (AuNCs), silver nanoclusters (AgNCs), and copper nanoclusters (CuNCs). The DNA probes could effectively be applied as the template for the preparation of fluorescent metal nanoclusters. In the presence of the HBV target, the trans-cleavage capability of Cas12a was able to be initiated to degrade DNA probes, which brought about the inhibition of metal nanoclusters formation, thereby giving minimal fluorescence signals. In comparison with AuNCs and AgNCs, the CuNCs-based nanosensor exhibited much higher sensitivity. In addition, the fluorescence response of CuNCs to HBV detection was significantly faster, which could be completed within 25 min without the requirements of sophisticated equipment or harsh reaction conditions. Moreover, the practical application of this analysis method was also demonstrated to detect HBV DNA target in human serum samples with satisfactory recovery, which was promising for simple, rapid, sensitive, and label-free HBV DNA bioanalysis .
研究领域:
分子生物学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Qing Liu,Xu Yan,Qi Lai,Xingguang Su
Bimetallic nanoclusters , compared to monometallic nanoclusters, possess better chemical performance. Herein, with the assistance of chondroitin sulfate , a novel fluorescent gold/silver nanoclusters (Au/Ag NCs) have been developed and applied to establish a sensing platform for sensitive detection of hyaluronidase (HAase). The Au/Ag NCs fluorescence intensity can be distinctly quenched by gold nanoparticles (Au NPs) due to the inner-filter effect (IFE). With the introduction of protamine (PRO), the positively charged PRO can bind to negatively charged Au NPs via electrostatic attraction resulting in the aggregation of gold nanoparticles. When PRO was preferential interaction with the negatively charged hyaluronic acid (HA), it would lead to the disaggregation of Au NPs and quench the fluorescence again. However, HAase can hydrolyze HA into small fragments which prevented HA interacting with protamine, and leading to the aggregation of Au NPs and weaken the IFE of Au NPs on Au/Ag NCs. Thus, the fluorescence of bimetallic nanoclusters was “turn-on”. This “turn-on” sensing system can be utilized to detect HAase level in the range of 0.5–37.5 U mL −1 . The developed strategy not only expands the application scope of bimetallic nanoclusters, but also provides a new approach for the determination of HAase.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2019
·Vol. 11
·期 8
·1139-1144
·
IF 0
Gu Mengqiao,Liu Jiali,Li Delei,Wang Min,Chi Kuanneng,Zhang Xi,Deng Yan,Ma Yuchan,Hu Rong,Yang Yunhui
In this paper, a signal-off aptasensor was constructed based on the specificity of OTA and OTA aptamer (DNA2), it can detect the small molecule ochratoxin A (OTA). DNA1 chain with mercapto modified at 3′-end was firstly immobilized on gold electrode, which cross-fertilize with
DNA 2 to form double strands. 30 thymine T modified at the end of DNA2 were used as templates to synthesize Cu NCs which is peroxidase mimics and can catalyze hydrogen peroxide. Presence of OTA, it specifically binds to DNA2, which made DNA2 dissociated, and so did Cu NCs. The more OTA in
solution, the more the aptamers and Cu NCs dissociated from the gold electrode, the less copper nanoclusters remained on the electrode, and the smaller the current response value of the electrode measured by chronoamperometry. Thus, the quantitative determination of OTA can be achieved. This
aptasensor provides a simple and fast technique for OTA detection in red wine samples.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
生物传感器
✓ 已验证
DOI
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Xinyi Wang,Zhu Su,Liang Li,Yifeng Tu,Jilin Yan
Molybdenum (Mo) is an essential nutrient for the proper functioning of some enzymes in living organisms as human beings. Conventional methods for its detection require complicated instrumentations as atomic absorption or mass spectrometers. In this work, a sensitive kinetic fluorescence was developed as an alternative. Gold nanoclusters (AuNCs) with red fluorescence emission were synthesized, and this fluorescence was effectively quenched by iodine through an etching process. It was found that the presence of Mo significantly speeded up a reaction for the generation of iodine, and thus enhanced the quenching. This effect was then adopted for the development of a sensitive fluorescent measurement toward Mo. The method was capable of detecting Mo down to 0.2 nM and was successfully applied for the analyses of mung bean and tea leaf samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2021
·
IF 3.08
Yu Chen,Ting Feng,Li Chen,Yiming Gao,Junwei Di
A “signal-on” fluorescence method for determination of hydrogen peroxide (H 2 O 2 ) and glucose was developed using bovine serum albumin-capped Ag nanoclusters with thirty atoms (BSA-Ag 30 NCs) as sensing probe. It was relied upon the size-dependent fluorescence of BSA-AgNCs. The prepared BSA-Ag 30 NCs have an average diameter of ~3.4 nm and exhibit a red emission at 635 nm. The fluorescence intensity of BSA-AgNCs could be enhanced by H 2 O 2 with the etching and then formation of smaller nanoclusters. Under the optimum experimental conditions, a linear calibration curve for H 2 O 2 was obtained within the concentration range of 2–200 μM with detection limit of 1.1 μM. Coupling with glucose oxidase (GOD) catalytic reaction, the BSA-Ag 30 NCs sensing platform could also be used to detect glucose. The enhancement of fluorescence intensity (F–F 0 )/F 0 was linear related to the concentration of glucose over the range 5–180 μM with detection limit of 3.4 μM. This method was successfully applied to the determination of glucose in human serum samples with satisfactory result.
研究领域:
分析化学
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）