ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Tingting Liu,Ruiyong Zhou,Kechen Wu,Gangbing Zhu
Nanozymes were emerged as the next generation of enzyme-mimics which exhibit great applications in various fields, but there is rarely report in the electrochemical detection of heavy metal ions . In this work, Ti 3 C 2 T x MXene nanoribbons@gold (Ti 3 C 2 T x MNR@Au) nanohybrid was prepared firstly via a simple self-reduction process and its nanozyme activity was studied. The results showed the peroxidase-like activity of bare Ti 3 C 2 T x MNR@Au is extremely weak, while in the presence of Hg 2+ , the related nanozyme activity is stimulated and improved remarkably, which can easily catalyze oxidation of several colorless substrates (e.g., o-phenylenediamine) to form colored products. Interestingly, the product of o-phenylenediamine exhibits a strong reduction current which is considerably sensitive to the Hg 2+ concentration. Based on this phenomenon, an innovative and highly sensitive homogeneous voltammetric (HVC) sensing strategy was then proposed to detect Hg 2+ via transforming the colorimetric method into electrochemistry since it can exhibit several unique advantages (e.g., rapid responsiveness, high sensitivity and quantificational). Compared to the conventional electrochemical sensing methods for Hg 2+ , the designed HVC strategy can avoid the modification processes of electrode coupled with enhanced sensing performances. Therefore, we expect the as-proposed nanozyme-based HVC sensing strategy provides a new development direction for detecting Hg 2+ and other heavy metals.
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Shiqi Xu,Songtao Wang,Ling Guo,Yuqin Tong,Lina Wu,Xingxu Huang
CRISPR-based detection was often based on the target preamplification to realize the high sensitivity. Here, we prepared a CRISPR-Cas12a system for the colorimetric detection of lead ion (Pb 2+ ) based on the assistance of DNAzyme and nanozyme instead of preamplification. The recognition between GR-5 DNAzyme and Pb 2+ could trigger the CRISPR-Cas12a system. MnO 2 nanozymes connected with magnetic beads through single stranded DNA were prepared as the colorimetric signal probes and catalyst of CRISPR-Cas12a system for the strong oxidase-like activity inducing the color change of 3,3′,5,5′-tetramethylbenzidine. The nanozyme-catalysed CRISPR-Cas12a system could be used to detect Pb 2+ through the color change with high specificity and sensitivity. The linear range of this approach was 0.8 nM–2500 nM, with a limit of detection of 0.54 nM. This method was applied for the detection of the Pb 2+ in food samples indicating good accuracy and anti-interference ability.
研究领域:
生物传感器
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分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Linxue Zhen,Zhichun Bi,Xinyue Zhang,Peize Jin,Donghui Song,Hui Huang,Yongxin Li
A nanozyme sensor array was developed combining with machine learning algorithms for the effective identification and differentiation of multiple antibiotic classes and antibiotic individuals. The four channel sensor array was composed by four copper-based nanozymes with laccase-like activity. The significant structural differences between different classes of antibiotics (such as Aminoglycosides, Tetracyclines, β-lactams, Nitroimidazole, Sulfonamides, Quinolones) resulted in the different effect on the nanozymatic reactions through interact with copper、substrate and so on. In addition, the differences between antibiotics in the same class also made certain effect on the nanozymatic reactions. Based on the above affect and differences, we combined the machine learning algorithms to successfully realize the identification of six major classes and sixteen individuals of antibiotics simultaneously for the first time. The proposed sensing strategy realized the facile identification of major categories of antibiotics, which has good application prospects in the rapid antibiotic identification in environmental water bodies.
研究领域:
生物化学
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环境科学
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医学检测
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jun Li,Zue Hu,Yi Peng,Hui Yang,Qian Zhou,Nan Wang,Xianggui Chen,Xiaoqi Yu
Nanozymes have become one of the key applications in the analysis and sensing of food. However, nanozymes presented have shortcomings that limit their further application, such as complex raw material sources, and high condition requirements for synthesis. Cu-GSSG nanozymes under green preparation conditions were synthesized inspired by the detoxification process of glutathione in living organisms. Cu-GSSG nanozymes were further loaded onto filter paper to construct a smartphone-assisted colorimetric sensor test strip for ascorbic acid (AA) detection. As the excellent peroxidase-like enzymes, Cu-GSSG nanozymes showed low AA detection limit of 2.9 μM with a wide linear range of 10–300 μM. Cu-GSSG nanozymes exhibited excellent anti-interference, accuracy, and biocompatibility performances for reliable detection AA in prickly pear juice and 10 supplements. The intelligent colorimetric sensor platform based on the bio-inspired Cu-GSSG nanozymes showed the great potential in rapid detection and large-scale screening of AA in fruits and supplements.
研究领域:
食品分析
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生物医学工程
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纳米技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Runzi Zhang,Jia Yang,Yongguo Cao,Qianyan Zhang,Chenfeng Xie,Wanyi Xiong,Xiaojun Luo,Yi He
Biomimetic inorganic nanoenzyme is a kind of nanomaterial with long-term stability, easy preparation and low cost, which could instead of natural biological enzyme. Metal-organic framework (MOFs) as effectively nanoenzyme was attracted more attention for the adjustability and large specific surface area. This design is based on the catalase-like catalytic activity of 2D metal–organic frameworks (MOFs) and the high sensitivity of surface enhanced Raman spectroscopy (SERS) biosensors to construct a novel SERS biosensor capable of efficiently detecting mercury (Hg 2+ ). In this study, 2D MOFs nanozyme was instead of 3D structure with more effecient catalytic site, which can catalyze o-Phenylenediamine (OPD) to OPDox with the assistance of H 2 O 2 . Besides, a magnetic composite nanomaterial Fe 3 O 4 @Ag@OPD was prepared as a signal carrier. In the presence of Hg 2+ , T-Hg 2+ -T base pairs were used to connect the two materials to realize Raman signal change. Based on this principle, the SERS sensor can realize the sensitive detection of Hg 2+ , the detection range is 1.0 × 10 -12 ∼ 1.0 × 10 -2 mol‧L -1 , and the detection limit is 1.36 × 10 -13 mol‧L -1 . This method greatly improves the reliability of SERS sensor for detecting the target, and provides a new idea for detecting metal ions in the environment.
研究领域:
生物化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2019
·Vol. 5
·期 5
·
IF 12.804
Liang Huang,Jinxing Chen,Linfeng Gan,Jin Wang,Shaojun Dong
Conventional nanozyme technologies face formidable challenges of intricate size-, composition-, and facet-dependent catalysis and inherently low active site density. We discovered a new class of single-atom nanozymes with atomically dispersed enzyme-like active sites in nanomaterials, which significantly enhanced catalytic performance, and uncovered the underlying mechanism. With oxidase catalysis as a model reaction, experimental studies and theoretical calculations revealed that single-atom nanozymes with carbon nanoframe–confined FeN 5 active centers (FeN 5 SA/CNF) catalytically behaved like the axial ligand–coordinated heme of cytochrome P450. The definite active moieties and crucial synergistic effects endow FeN 5 SA/CNF with a clear electron push-effect mechanism, as well as the highest oxidase-like activity among other nanozymes (the rate constant is 70 times higher than that of commercial Pt/C) and versatile antibacterial applications. These suggest that the single-atom nanozymes have great potential to become the next-generation nanozymes.
研究领域:
纳米技术
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催化
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材料科学
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生物无机化学
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抗菌应用
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DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 9
·892-899
·
IF 3.532
Haotian Zhong,Yuting Xue,Bin Liu,Zhengbo Chen,Kai Li,Xia Zuo
Phenols are harmful to the human body and the environment. Since there are a variety of phenols in actual samples, this requires a sensor which possesses the ability to simultaneously distinguish them. Herein, we report a colorimetric sensor array, which uses two nanozymes (Fe–N–C nanozymes and Cu–N–C nanozymes) as electronic tongues for fingerprint identification of six phenols (2,4,6-trichlorophenol (2,4,6-Tri), 4-nitrophenol (P-np), phenol (Phe), 3-chlorophenol (3-CP), 4-chlorophenol (4-CP), and o-nitrophenol (O-np)) in the environment. Nanozymes catalyzed the reaction of hydrogen peroxide, different phenols and 4-aminoantipyrine (4-AAP) to produce different color variations. These signal changes as fingerprints encouraged us to develop a pattern recognition method for the identification of phenols by linear discriminant analysis (LDA). The six phenols at 50 nM have their own response patterns, respectively. Surprisingly, this sensor array had distinguished the six phenols in actual samples successfully.
研究领域:
分析化学
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环境科学
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纳米技术
·
传感器
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产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2023
·Vol. 9
·期 10
·e202300310
·
IF 3.8
Xin Cui,Jiachen Zhang,Yitong Liu,Wen Shao,Yawen Tang,Hanjun Sun
Graphical A Ru-ZIF-8 nanozyme with both esterase like and peroxidase like activities were prepared for acetylcholine (ACh) detection. On the basis of the Ru-ZIF-8 nanozyme, a portable nanozyme strip was developed as a novel biosensor for detecting ACh, which was conducive to promoting the development of multiple enzyme cascade assays. In the present study, the incorporation of Ru into the framework of ZIF-8 resulted in that a Ru-ZIF-8 nanozyme with both esterase-like activity and peroxidase-like activity. Owing to the dual nanozymatic activities of Ru-ZIF-8 nanozyme, the acetylcholine (ACh) detection process utilized only one natural enzyme (choline oxidase), which greatly simplified the detection procedure. In the detection process, nanozyme Ru-ZIF-8 showed esterase-like activity to catalyze the hydrolysis of ACh to choline. Then the generated choline was oxidized by choline oxidase to generate H 2 O 2 . Finally, Ru-ZIF-8 with peroxidase-like activity was used to catalyze H 2 O 2 to oxidize 2,2′-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) ammonium salt with a color change. As a result, the linear range for ACh was from 0.01 μM-22 μM with the detection limit (DL) of 5.5 nM. In addition, Ru-ZIF-8 was utilized to fabricate a portable detection test strip. Our work provided a simple, portable, low-cost, highly sensitive and selective assay for the detection of ACh, which paved the way for the development of multi enzyme cascade assays.
研究领域:
生物化学
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纳米技术
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分析化学
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生物传感器
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Lin Fan,Lijun Kong,Hao Liu,Jiawei Zhang,Mengdi Hu,Li Fan,Hongliang Zhu,Shancheng Yan
Ag and Cu based nanostructures serve as advanced functional materials for biomedical applications , due to their unique properties. Here, we proposed a novel neurotransmitter biosensing method based on Ag–Cu composite nanozyme , synthesized through the soft film plate method. Supported by the soft film template , the Ag–Cu nanozymes were stably kept to an ultrafine 2D structure with high monodispersity, which provided a large specific surface area and sufficient binding sites , leading to controllable and improved dual-nanozyme activities over similar-sized mono-Ag and mono-Cu, and up to 4.95 times of natural enzyme-level. The multi-path enzymatic reaction processes catalyzed by Ag–Cu composite nanozymes were firstly theoretically discussed in detail, according to the theoretical redox potential of redox couples in the reaction systems. On this basis, the Ag–Cu filled nanonets based neurotransmitter biosensing is successfully applied in rapid detection for glutathione and dopamine, possessing a linear range of 10∼100 μM and 1–10 μM, and a detection limit of 3.01 μM and 0.29 μM, respectively, which exhibited superior performance for biomedical purposes over most commercially available products in speed and precision.
研究领域:
生物医学工程
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材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Shengnan Zhang,Hongxian Zhou,Xin Liu,Yantong Wu,Shuran Yang,Jia Yin,Mohammad H. Shubair,Mingzhu Zhang
Periodontitis, a chronic inflammatory disease, leads to the progressive destruction of tooth-supporting tissues, and current treatments fall short of fully restoring periodontal regeneration. Dental pulp stem cells (DPSCs) have shown great promise for periodontal regeneration due to their ability to differentiate into multiple cell types and modulate immune responses. However, clinical use of DPSCs remains challenging due to poor cell retention, dependence on scaffolds, and high costs. Additionally, excessive reactive oxygen species (ROS) in the periodontal microenvironment hinder stem cell proliferation and differentiation, further limiting their regenerative potential. To address these challenges, we developed ultrafine cerium oxide nanoparticles (CeO2NPs) and engineered DPSCs (E-DPSCs) by incorporating these antioxidant nanozymes via endocytosis. The modified E-DPSCs exhibited enhanced proliferation, migration, and osteogenic differentiation under normal conditions. Crucially, under oxidative stress, E-DPSCs effectively neutralized ROS, reduced apoptosis, and maintained their osteogenic capacity. In vivo studies further demonstrated that E-DPSCs significantly enhanced periodontal regeneration compared to unmodified DPSCs. These findings highlight E-DPSCs as a promising solution to overcome current limitations in stem cell therapy, offering a more effective strategy for periodontal repair and regeneration.
研究领域:
牙科学
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纳米医学
·
干细胞研究
·
再生医学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Xueyun Wen,Tao Chen,Jinping Yu,Hao Shi,Lupeng Wang,Kaikai Xu,Yuanhong Xu
Endotoxin detection is important for determining bacterial contamination and infection in fields of food, pharmaceutical and clinical disease diagnosis. The horseshoe crab deformed cell lysate analysis is regarded as the gold-standard method, but the endangered and high-cost horseshoe crab animals required in sensing process further raise animal ethical issues and hinder their applications. The colorimetric methods based on nanozymes are simple and economical, but the low selectivity and sensitivity are still the bottleneck for their further application. Herein, we successfully developed a phenylboronic acid functionalized iron-based nanozyme with higher selectivity and highly catalytic activity for endotoxin sensing. The as-prepared colorimetric sensor using the obtained nanozyme as sensing probes shows a good linear relationship for endotoxin sensing in the range of 1–20 μg mL −1 , with a LOD = 0.42 μg mL −1 , along with good selectivity and reproducibility. The sensor can also be well applied to detecting endotoxin in practical samples such as beer and serum. Moreover, the parameters including time and temperature which could affect the endotoxin release from E. coli were also studied and optimized, based on the relationship between endotoxin and Gram-negative bacteria, the as-prepared sensor achieves the qualitative and quantification of E. coli .
研究领域:
生物技术
·
纳米技术
·
分析化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2019
·
IF 1.267
Kai Sun,Shun-Yao Li,Hui-Ling Chen,Qing-Guo Huang,Youbin Si
In this study, we used a simple and rapid colourimetric reaction for visual sensing of Fe2+ and Pb2+ ions in water by employing nano-MnO2 as a natural oxidase mimic to respectively catalyse ABTS and TMB in citrate-phosphate buffer solution (C-PBS) at 25°C and pH 3.8. It was found that nano-MnO2 possessed highly oxidase-mimicking activity with the Km values of 0.030 and 0.027 toward ABTS and TMB, respectively, indicating TMB had a stronger affinity on nano-MnO2 than ABTS. Interestingly, the presence of 0.01 mmol·L−1 Fe2+/Pb2+ ion was able to significantly down-regulate the activity of MnO2 nanozyme in nano-MnO2-mediated ABTS reaction processes (P < 0.01), which mainly due to the strong adsorption of metal ion toward nano-MnO2 surface via the electrostatic attractions, thus leading to the passivation and inactivation of MnO2 nanozyme catalytic activity. Thereinto, Fe2+ reacted with multivalent manganese by oxidation-reduction, while Pb2+ was specifically adsorbed onto the surface of MnO2 nanozyme and formed complexes. Notably, only Fe2+ ion inhibited the activity of MnO2 nanozyme-TMB with a detection limit as low as 1.0 μmol·L−1. In MnO2 nanozyme-ABTS sensing systems, Fe2+ and Pb2+ ions detection limit of 0.5 and 2.0 μmol·L−1 were, respectively, achieved with a linear response range of 0–0.02 and 0–0.8 mmol·L−1, implying the developed MnO2 nanozyme-ABTS sensor was potentially applicable for the visual determination of Fe2+ and Pb2+ ions in water. In the real water samples, MnO2 nanozyme-ABTS achieved high accuracy (relative errors: 3.4‒10.5%) and recovery (96‒110%) for respective detection of Fe2+ and Pb2+ ions. The simple and rapid MnO2 nanozyme-ABTS sensing systems might provide a practical assay for visual detection of Fe2+ and Pb2+ ions in the environmental water samples.
研究领域:
分析化学
·
环境科学
·
生物催化
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）