Journal of Cleaner Production
·
2024
·
IF 9.7
Xuqiang Zhao,Lei Tang,Xuwen Chen,Jian Wang,Yanzheng Gao
Phthalic acid esters (PAEs) as common plasticizers, providing convenience to humans. However, they also present a potential threat to ecosystems and human health as emerging contaminants. In this study, co-immobilization of nanozyme (PtCo) and laccase (Lac) into dendritic mesoporous silica (DMSN) constitutes nanomaterials (Lac@PtCo@DMSN). PtCo with an atomic ratio of 3:1 has the highest oxidizing activity. DMSN exhibited a maximum Lac loading capacity of 365 mg/g and activity of Lac@PtCo@DMSN exhibits excellent pH, temperature, and storage stability and reusability, and demonstrated greater recoverability. Remarkably, Lac@PtCo@DMSN (50 mg) achieved 81.83% degradation of total PAEs within 72 h and maintained 72.44% degradation of dimethyl phthalate (DMP) in the fifth cycle. Electron transfer between Pt, Co and adsorbed oxygen (O 2ads ) in PtCo can generate •OH and •O 2 − , which were the dominant reactants for PAEs degradation. •OH could degrade PAEs to phthalic acid via H-abstration and •OH addition. •OH effectively enhanced Lac activity, and promoted the synergistic catalytic performance of nanozymes and natural enzymes. The degradation of phthalic acid by Lac accelerated the release of PtCo@DMSN reaction sites, resulting in significantly enhanced PAEs degradation. Thus, artificial/natural enzymes can degrade PAEs without exogenous additives to solve the limitation of Lac application, providing a new strategy for degrading contaminants in water.
研究领域:
环境科学
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生物化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Miaomiao Li,Xiuying Peng,Yujie Han,Lifang Fan,Zhiguang Liu,Yujing Guo
MXenes are attractive for various biosensors owning to their tunable versatile properties. Herein, Ti 3 C 2 nanosheets (Ti 3 C 2 NSs) were found to possess intrinsic peroxidase-like activity, and which could be enhanced by single-stranded DNA (ssDNA) adsorbed on the nanosheets. Hence, a simple label-free sensing strategy was developed for colorimetric detection of biomolecules, based on Ti 3 C 2 NSs as a peroxidase mimic enzyme and ssDNA aptamer as an enzymatic activity enhancement factor along with a recognition substance for the target. In the presence of target biomolecules, the aptamers were desorbed from Ti 3 C 2 NSs due to the specific binding of targets to aptamers, thus bringing about the decrease of catalytic activity. Thrombin (TB) as the model was determined by this constructed biosensor with a linear range of 1.0 × 10 −11 to 1.0 × 10 −8 M, and evaluated subsequently in the real blood samples with satisfactory results. This work highlights Ti 3 C 2 MXenes as a nanomaterial mimic enzyme (called nanozyme) for monitoring biomolecules by target-specific recognition strategy.
研究领域:
生物传感
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材料科学
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医学检测
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Jie Meng,Wei Wei,Shuang Xie,Junwu Wei,Zhanlin Zhang,Pan Ran,Xiaohong Li
Pyroelectrodynamic therapy (PEDT) has emerged as an attractive therapeutic modality for tumors, but is dramatically discounted by inherent defects of wide band gap, fast electron − hole recombination and low photothermal utilization. Herein, hollow-structured Janus nanoparticles (NPs) are developed to improve tumor accumulation and PEDT generation of reactive oxidative species (ROS) for antitumor treatment. Hollow barium titanate (hBT) NPs were prepared by the template and hydrothermal method for in situ deposition of CuS nanodots and then partially coated with polydopamine (PDA) caps to obtain hBT-CuS@PDA. Finite element analysis reveals that the unique thin shell of hBT benefits transportation of pyroelectrically generated charge carriers through shortening diffusion distance to the interface. The CuS deposition forms heterojunctions with hBT to accelerate electron − hole separation and leads to a larger pyroelectric current. The hollow structure facilitates light absorption and conversion, and the photothermal effect of the half-coated PDA caps generates asymmetric temperature gradient to drive NP locomotion across biological barriers. The in situ generated pyroelectric field selectively affects membrane potential and fluidity of tumor cells to enhance the specific NP internalization. The pyroelectric effect enhances the peroxidase-like activity of CuS nanodots to generate toxic ·OH and the glutathione peroxidase-like activity to inhibit glutathione consumption of ROS. The integration of photothermal, pyroelectric, nanozyme catalysis and self-protrusion effects promotes NP accumulation and penetration throughout tumors to exhibit therapeutic effect at a mild-temperature, reducing the possible high-temperature ablation of normal tissues. The hollow and heterjunction structure generates abundant intracellular ROS and efficient diffusion in tumor tissues to inhibit tumor growth and extend animal survival, offering a safe and effective PEDT strategy.
研究领域:
生物医学工程
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纳米医学
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肿瘤学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 47
·11235-11250
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IF 7
Chan Chen,Haisheng Ren,Weikang Tang,Mengqi Han,Qinfei Chen,Hong Zhou,Jiadong Chen,Yuyue Gao,Wenbin Liu
L-3,4-Dihydroxyphenylalanine (L-DOPA) is widely used in Parkinson's disease treatment and is therefore in high demand. Development of an efficient method for the production of L-DOPA is urgently required. Nanozymes emulating tyrosine hydroxylase have attracted enormous attention for biomimetic synthesis of L-DOPA, but suffered from heterogeneity. Herein, a spherical porous iron–nitrogen–carbon nanozyme was developed for production of L-DOPA. Tannic acid chelated with ferrous ions to form a tannin-iron coordination framework as a carbon precursor. Iron and nitrogen co-doped carbon nanospheres were assembled via an evaporation-induced self-assembly process using urea as a nitrogen source, F127 as a soft template, and formaldehyde as a crosslinker. The nanozyme was obtained after carbonization and acid etching. The nanozyme possessed a dispersive iron atom anchored in the Fe–N coordination structure as an active site to mimic the active center of tyrosine hydroxylase. The material showed spherical morphology, uniform size, high specific surface area, a mesoporous structure and easy magnetic separation. The structural properties could promote the density and accessibility of active sites and facilitate mass transport and electron transfer. The nanozyme exhibited high activity to catalyze the hydroxylation of tyrosine to L-DOPA as tyrosine hydroxylase in the presence of ascorbic acid and hydrogen peroxide. The titer of DOPA reached 1.2 mM. The nanozyme showed good reusability and comparable enzyme kinetics to tyrosine hydroxylase with a Michaelis–Menten constant of 2.3 mM. The major active species was the hydroxyl radical. Biomimetic simulation of tyrosine hydroxylase using a nanozyme with a fine structure provided a new route for the efficient production of L-DOPA.
研究领域:
生物化学
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材料科学
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医学
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DOI
PubMed
高影响因子
Chemosensors
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2024
·Vol. 12
·期 6
·104
·
IF 3.7
Yanyan Xing,Pingping He,Deyong Wang,Yuan Liang,Xing Gao,Xiaohong Hou
Colorimetric biosensor-based nanozymes have received considerable attention in various fields thanks to the advantages of the simple preparation, good stability, and regulable catalytic activity of nanozymes. In this study, a bimetallic nanozyme Fe3O4@Co3O4/CN was prepared via the high-temperature calcination of Fe3O4-PVP@ZIF-67. The material retained its skeletal structure before calcination, which prevented the aggregation of nanoparticles and exposed more active sites of the nanozyme, substantially enhancing the intrinsic dual enzyme-mimetic activities, including peroxidase- and oxidase-like activities. In particular, Fe3O4@Co3O4/CN with oxidase-like activity catalyzed the colorless tetramethylbenzidine (TMB) to become blue oxTMB with oxygen. Reducing glutathione (GSH) could inhibit the above oxidation reaction. In contrast, with respect to the existence of mercury(II), GSH bound to mercury(II) due to the strong affinity between mercury(II) and -SH, thus eliminating the inhibition and restoring the oxTMB signal. A simple and effective colorimetric sensor was fabricated to detect mercury(II) based on the above principles. The proposed measurement had a linear range of 0.1–15 μM and a limit of detection (LOD) of 0.017 μM. It was shown that the established colorimetric sensing system could be successfully applied to detect mercury(II) in water samples, and the Fe3O4@Co3O4/CN nanozyme proved to be a promising candidate for biosensing application.
研究领域:
生物传感
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材料科学
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环境监测
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Yang Xuan,Yating Gao,Yinan Zhao,Wenliang Zhang,Xinyu Bian,Mingfan Zhang,Ruoyun Zhang,Shubiao Zhang
Nanomaterials with intrinsic enzyme activity (nanozymes) have been widely used as artificial enzymes in biomedicine, especially in tumor diagnosis and treatment. Herein, a kind of water-soluble silver nanozyme (Ag@GQD) was developed with a simple method, which showed two enzymatic activities (peroxidase and oxidase-like activity). Based on the excellent catalytic performance of oxidase-like activity, a simple and highly sensitive colorimetric detection strategy for glutathione (GSH) was established. What’s more, after absorbing DOX, Ag/DOX@GQD could realize the combined treatment of reactive oxygen species and chemotherapy. In conclusion, this study provided evidences that Ag@GQD is effective in tumor diagnosis and treatment, which provided new ideas for exploring nanozymes based on the integration of diagnosis and treatment.
研究领域:
生物医学
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纳米医学
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肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Haixia Hu,Jiangtao Hu,Kang Xue,Hong Zhou,Xiao Yang,Lijun Wang,Xianggui Chen
Due to their superparamagnetism and enzyme-like activity, iron oxide (Fe 3 O 4 ) nanozymes can be readily used for sample pretreatment and the generation of detection signals, and have, thus, attracted much attention in the field of bioanalysis and diagnosis. However, the low catalytic activity of Fe 3 O 4 nanozymes does reduce the sensitivity of Fe 3 O 4 -based methods, limiting their application. In this study, Fe 3 O 4 @Cu@poly(pyrrole-2-carboxylic acid) yolk-shell nanozymes (Fe 3 O 4 @Cu@PCPy YSNs) were synthesized using a facile approach and selective chemical etching technology . Compared with Fe 3 O 4 nanozymes, the Fe 3 O 4 @Cu@PCPy YSNs demonstrated a three-fold increase in the peroxidase-like activity, good dispersity and strong superparamagnetism. In addition, the flower-shaped structure of aptamer-complementary strand (Apt-CS) conjugates was designed on the surface of the Fe 3 O 4 @Cu@PCPy YSNs, which effectively inhibited their peroxidase-like activity by creating a physical barrier that hindered the access of substrates to the center of the Fe 3 O 4 @Cu@PCPy YSNs. Based on this principle, a robust and facile colorimetric aptasensor was developed for detecting Salmonella Typhimurium. The flower-shaped Apt-CS were dissociated in the presence of S . Typhimurium, promoting the recovery of Fe 3 O 4 @Cu@PCPy YSN catalytic activity. Under optimized conditions, this proposed aptasensor successfully detected S . Typhimurium in a linear range of 3 to 3 × 10 6 CFU/mL, achieving a detection limit of 1 CFU/mL. Finally, the feasibility of this novel aptasensor was further validated by three actual samples, with recoveries of between 84.3% and 102%, thereby demonstrating the huge potential of the proposed aptasensor for detecting S . Typhimurium in foods.
研究领域:
生物分析
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诊断学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Li-Xia Yan,Zhu-Ying Yan,Xu Zhao,Li-Jian Chen,Xiu-Ping Yan
Total antioxidant capacity (TAC) is a vital parameter to assess the gross antioxidant activity in biological and food matrices. Accurate determination of TAC has become increasingly important for health monitoring and dietary guidance. Here, we report a persistent luminescent nanozyme (ZGO-Pt) based luminescence-colorimetric dual-mode sensor for selective determination of TAC. ZGO-Pt was fabricated based on ZnGa 2 O 4 :Cr nanoparticles (ZGO) with platinum nanoflower as synthetic additive. The prepared ZGO-Pt exhibited fascinating peroxidase-mimetic activity and persistent luminescence, and enabled effectively catalysis of the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. Meanwhile, the generated oxTMB gave strong absorption at 652 nm to quench the luminescence of ZGO-Pt at 700 nm via the inner filter effect. Antioxidants could compete with the oxidable TMB or reduce oxTMB to TMB, resulting in the simultaneous variations of blue color and luminescence to provide the basis for dual-mode assay for TAC. The highly efficient catalysis of ZGO-Pt ( K m , 3.12 mM for H 2 O 2 , 0.592 mM for TMB) allows the dual-mode sensor to realize precise determination of TAC with a wide linear range (0.5–200 μM and 1–300 μM for colorimetric and luminescence mode, respectively) and low limit of detection (LOD, 0.05 μM and 0.78 μM for colorimetric and luminescence mode, respectively). The developed dual-mode sensor was successfully applied to the determination of TAC in vitamin tablet, beverages and fruits. This work provides an efficient persistent luminescent nanoparticle-based nanozyme for a facile TAC assay with further vision in biosensing and food technology.
研究领域:
生物化学
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分析化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Lulu Kong,DeZhu Chen,Xiaolong Zhang,Lin Zhou,Yongming Deng,Shaohua Wei
The growth of oxides on the surface of noble metals and the introduction of alloys are effective methods to enhance the catalytic activity of noble metals. However, the fabrication of noble metal oxides always involves harsh experimental conditions and the oxidation degree could not be controlled. Herein, a simple, fast, and controlled Pd oxidation method by mixing Hg2+ and Pd nanosheets (PdNSs) under mild conditions to form PdxHgy(PdO)z (PHOs) was reported, which consists of the Pd–Hg alloy and PdO. The density functional theory (DFT) calculation and experiment results proved that the Hg atom incorporation promotes the nearby Pd–Pd bond breaking, initiating a cascade of PdO generation. More precisely, 1 Hg atom incorporation induced ∼8 Pd atom oxidation, ensuring that the oxidation degree of Pd could be easily controlled by adjusting the ratio of Hg2+ and PdNS. As a peroxidase-mimicking nanozyme, the PHO affinity for its substrate could be 21.7- and 123.6-fold greater than that of the PdNS and the natural peroxidase (HRP), respectively. For the colorimetric immunoassay of carcinoembryonic antigen (CEA) detection, PHO has an ultralow detection limit of 1.33 pg mL–1, which is ∼8.6 and ∼282 times lower than that of the PdNS and the natural HRP, respectively.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Jingyuan Guo,Chenxin Dong,Xiang Zhang,Yujia Liu,Yumin Leng,Guo Wang,Zhengbo Chen
Background In recent years, environmental pollution has attracted widespread global attention. Among them, environmental problems caused by heavy metal pollution pose a serious threat to human health and ecosystems. Mercury is a common heavy metal pollutant with high toxicity and wide distribution. Excessive intake of Hg 2+ can cause permanent and severe damage to the nervous system, respiratory system, and kidneys in the human body. Therefore, developing both accurate and fast detection methods for Hg 2+ is of great significance. Results A sensitive Hg 2+ colorimetric sensor is designed based on PtNi nanowires (NWs) and Pt NWs with peroxidase-mimetic activity. PtNi NWs and Pt NWs catalyze the reaction of 3,3′, 5,5′-tetramethylbenzidine (TMB) with hydrogen peroxide (H 2 O 2 ) to produce blue oxidized TMB (oxTMB). The specific interaction of Pt–Hg significantly inhibits the peroxidase-mimetic activity of PtNi NW and Pt NW nanozymes, resulting in a lighter blue color. It is worth noting that compared with specific activity (SA) of Pt NWs (3.31 U/mg), PtNi NWs own superior SA (10.43 U/mg), which inevitably leads to a wider linear range of Hg 2+ analysis (1 nM-200 μM) and a lower detection limit (0.6748 nM) for PtNi NWs-based colorimetric sensor, versus linear range (4 nM-5 μM) and LOD of 1.198 nM for Pt NWs-based colorimetric sensor, which are far below the Hg 2+ threshold (10 nM) for drinking water set by the US Environmental Protection Agency. Significance : The two nanozyme colorimetric sensors have been successfully used for the evaluation of Hg 2+ in complex river water and tap water. Due to the advantages of simple operation, fast response, and high sensitivity, colorimetric sensors have broad application prospects in environmental monitoring.
研究领域:
环境科学
·
生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Bangxiang Liu,Hengjia Zhu,Rulin Feng,Mengzhu Wang,Panwang Hu,Jianming Pan,Xiangheng Niu
Nanozymes have been intensively explored to develop analytical methods in the past decade. Nonetheless, most nanozymes lack the catalytic specificity toward substrates, which becomes the biggest barrier hindering their applications. How to achieve high-selectivity nanozyme sensing without the assistance of biological elements (aptamers, antibodies, etc.) is challenging. In this work, we introduced molecularly imprinted sites onto the Fe 3 O 4 nanozyme surface via a facile process, and developed a colorimetric assay for the highly specific determination of tetracycline (TC). Self-polymerization of dopamine under mild conditions was employed to conveniently construct the proposed Fe 3 O 4 @MIP, which possessed abundant channels for substrates access to the Fe 3 O 4 core, showing a peroxidase-mimetic activity of catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue species. When TC existed, it was specifically captured by the MIP shell and partially blocked the cavities for substrate access, and such that the catalyzed TMB chromogenic reaction was hampered. Based on the principle, highly selective colorimetric detection of TC against structural analogues was acquired, with a detection limit of 0.4 μM, and practical applications of the assay in environmental water matrices were also verified. Thanks to the magnetic characteristic, the molecularly imprinted nanozyme could be easily recovered and regenerated for recyclable sensing.
研究领域:
分析化学
·
纳米生物技术
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Juan Xia,Dan Zheng,Ping Han,Chengying Yang,Mengdie Wang,Chenchen Liu,Xu Sun,Qi Wang
Due to the high costs and relative instability of natural enzymes, highly efficient enzyme mimics have attracted much research interest in constructing nanozymes of biosensors. This study fabricated Co 9 S 8 nanozymes with diverse morphologies by adjusting the sulfur content in a facile and efficient solvothermal strategy using the Co-glycerate microsphere as the sacrificial template. Our findings revealed that sulfur-mediated surface reconstruction, especially at an optimal sulfur concentration, enables the fabrication of the M−Co 9 S 8 nanozyme with a hollow nanosphere structure. The nanozyme exhibits exceptional peroxidase-like activity. In the presence of H 2 O 2 , M−Co 9 S 8 nanozyme significantly enhanced the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), resulting in a pronounced color change, thereby enabling the colorimetric sensing of glutathione (GSH). The systematic studies, including radical scavengers and electron spin resonance experiments, confirm that superoxide free radicals (·O 2 − ) play a key role in catalytic reactions and exhibit a synergistic effect with holes ( h + ). Based on these active radicals and the optimized electron transfer efficiency of the hollow porous spherical shell structure, the M−Co 9 S 8 nanozyme showed a catalytic efficiency of 5.44 times higher than that of L-Co 9 S 8 solid nanospheres with low sulfur content. The strategy demonstrated high sensitivity, excellent specificity, and robust stability. It featured a 5-order linear range and a detection limit as low as 3.16 nM for the detection of GSH, rendering it highly promising for clinical sample detection. A deeper understanding of Co 9 S 8 ′s surface reconstruction could offer crucial insights for developing high-performance nanomaterials and biosensors, broadening their potential applications in biosensing and biomedicine.
研究领域:
生物传感
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）