Ting Cao,Zhongsheng Xu,Wenhua Dong,Hong Ma,Zhefeng Fan,Yun Liu
HSO 3 − is an important reactive sulfur species that maintains the normal physiological activities of living organisms and participates in a variety of redox homeostatic processes. It has been found that changes in HSO 3 − levels is closely related to the heat stroke phenomenon of the organism. Heat stroke causes damage to normal cells, which in turn causes damage to the body and even death. It is crucial to accurately monitor and track the physiological behavior of HSO 3 − during heat stroke. Herein, a ratiometric multifunctional fluorescent probe DRM-SO 2 with dual-targeting ability to rapidly and precisely recognize HSO 3 − being constructed based on the FRET mechanism. DRM-SO 2 has extra Large Stokes shift (216 nm), very high sensitivity (DL = 12.2 nM), fast response time and good specificity. When DRM-SO 2 undergoes Michael addition with HSO 3 − , the fluorescence emission peak was blue-shifted from 616 nm to 472 nm, and a clear ratiometric signal appeared. The interaction between lysosomes and mitochondria in maintaining cellular homeostasis was investigated by the dual-targeting ability of the probe using HSO 3 − as a mediator. DRM-SO 2 achieved successful targeting and real-time monitoring of exogenous and endogenous HSO 3 − in the cells. More importantly, imaging experiments in heat stroke mice revealed high HSO 3 − expression in intestinal tissues. This provides new ideas and research tools for early prevention of heat stroke-induced diseases such as intestinal injuries. In addition, the semi-quantitative monitoring experiments for paper-based visualization of HSO 3 − make the probe promising for the design of portable detectors.
Bingyan Han,Ying Li,Xixi Hu,Qin Yan,Jingmei Jiang,Mingbo Yu,Tingting Peng,Gaohong He
Fluorescence has been extensively employed for visual detection; however, it is inconvenient for people to distinguish single-color fluorescence changes. Herein, a dual-signal ratiometric fluorescent probe of a carbon quantum dot/gold nanocluster (CQD/Au NC) nanosystem for sensitive and selective detection of Ag+ and L-cysteine (Cys) was established. The CQDs with blue fluorescence at 472 nm served as a reference signal and Au NCs with orange fluorescence at 605 nm functioned as a reporter. From zeta potential measurements and TEM images, there was no indication of interaction between the two materials due to electrostatic repulsion. Upon adjusting the proportion of the CQDs and Au NCs, the nanosystem emitted a gray-orange emission at a single excitation wavelength. Ag+ enhanced the orange colour of Au NCs, but Cys could quench it completely with the high affinity of the coordination interaction between Cys and Ag+, while the blue fluorescence of CQDs was not affected. Therefore, the fluorescence of the nanosystem showed gray orange to light orange colour for determination of Ag+, and light orange to blue colour for determination of Cys. Moreover, paper-based visual detection was successfully carried out, which showed a more distinguishable color difference than that in aqueous solution. Our method has bright potential for application in the real-time and online visual detection of Ag+ and Cys in the future.
In this work, a novel time-resolved ratiometric fluorescent probe based on dual lanthanide (Tb: terbium , and Eu: europium)-doped complexes (Tb/ [email protected] 2 -Eu/GMP) has been designed for detecting anthrax biomarker (dipicolinic acid, DPA), a unique and major component of anthrax spores . In such complexes-based probe, Tb/ [email protected] 2 can serve as a stable reference signal with green fluorescence and Eu/GMP act as a sensitive response signal with red fluorescence for ratiometric fluorescent sensing DPA . Additionally, the probe exhibits long fluorescence lifetime , which can significantly reduce the autofluorescence interferences from biological samples by using time-resolved fluorescence measurement. More significantly, a paper-based visual sensor for DPA has been devised by using filter paper embedded with Tb/ [email protected] 2 -Eu/GMP, and we have proved its utility for fluorescent detection of DPA, in which only a handheld UV lamp is used. In the presence of DPA, the paper-based visual sensor, illuminated by a handheld UV lamp, would result in an obvious fluorescence color change from green to red, which can be easily observed with naked eyes. The paper-based visual sensor is stable, portable, disposable, cost-effective and easy-to-use. The feasibility of using a smartphone with easy-to-access color-scanning APP as the detection platform for quantitative scanometric assays has been also demonstrated by coupled with our proposed paper-based visual sensor. This work unveils an effective method for accurate, sensitive and selective monitoring anthrax biomarker with backgroud-free and self-calibrating properties.
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