JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Jincai Yang,Mingzhu Liu,Jin Wu,Tianyi Ma,Yanxuan Li,Yue Zhang,Jingran Sun,Xiaoli Li,Yanjun Fang,Yonghui Wang,Lingchao Cai,Yuan Peng,Zunquan Zhao,Jialei Bai
Antibiotics are emerging hazardous small molecules, requiring urgent need for fast signal-on analytical methods to control antibiotic abuse. Signal-on fluorescence sensing strategies utilizing nanoquenchers and aptamers are fascinating but rarely accomplished on paper-based platforms. Here, a novel multilayer MXene sensing platform was established by leveraging Nb 2 C-MXene as a multilayer nanoquencher and zero-dimensional carbon dots-labeled aptamer (B-CDs@Apt) as a stable and bright recognition probe. The Nb 2 C-MXene has a multilayer nanosheet stack-like structure and efficient mass transfer channels. It can efficiently adsorb abundant B-CDs@Apt probes and quench their fluorescence. Importantly, the Nb 2 C-MXene/B-CDs@Apt system can release the B-CDs@Apt in response to the analyte with high sensitivity, thereby restoring the fluorescent signal. The developed aptasensor achieved sensitive and selective detection of chloramphenicol (CAP) and showed satisfactory anti-interference ability, stability, and practicability. Notably, the multilayer Nb 2 C-MXene/B-CDs@Apt system was successfully transferred to a paper-based sensing platform, with a low-density distribution of multilayer nanoquenchers carrying sufficient aptamer probes for analyte access. In comparison, the monolayer Nb 2 C nanosheets were unable to adsorb enough probes to output analyte-induced signals. The established paper-based analytical device (PAD) showed a LOD of 0.360 ng mL −1 for CAP, which is the first paper-based MXene aptasensor reported for fluorescence detection. By replacing the aptamer and carbon dot, the strategy was further extended to detect another analyte oxytetracycline (OTC), with LODs of 0.399 in tube and 0.867 ng mL −1 on PAD, respectively. Furthermore, concurrent detection of CAP and OTC was achieved using a dual-color PAD, demonstrating the potential to meet multi-target analytical requirements.
研究领域:
分析化学
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生物传感
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环境科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Xinran Yang,Yuanyuan Liu,Yang Li,Ruixue Sun,Yiming Zhang,Donghui Wang
Rapid and accurate detection of trace cadmium ions (Cd 2+ ) in the environment and foodstuff is great of significance. Herein, we developed a fluorescent paper-based analytical platform for Cd 2+ detection with dual recognition modes based on the paper-based ion imprinting chip (Cd-IIP) and an aggregation-induced emission (AIE) probe (PBQ) for the first time. Cd-IIP was prepared based on sol–gel technique using 3-mercaptopropyltriethoxysilane (MPTES) as the functional monomer, Cd 2+ as the ionic template, and tetraethyl silicate (TEOS) as the cross-linking agent. Cd-IIP could selectively enrich Cd 2+ with good adsorption capacity (385.00 mg g −1 ) and imprinting factor (IF = 5.3). Benefiting from the PBQ with specific recognition of Cd 2+ based on metal coordination-induced AIE effect, the platform exhibited superior selectivity and recognition efficiency to Cd 2+ . The platform showed a wide linear range from 0.01 to 10 μg mL −1 with the limit of detection (LOD) as low as 0.0045 μg mL −1 under the synergy of the biomimetic recognition and AIE effect. Moreover, the smartphone-based analytical method was utilized for visual detection of Cd 2+ with the LOD of 0.11 μg mL −1 in real samples. Overall, this analytical platform possessed significant specificity and sensitivity, holding a great promise of dual selective principles for efficient analysis of Cd 2+ in complex systems.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Fang Yan,Rong Deng,Shaohui Guo,Xiaochun Li
Excessive pesticide residues have posed a severe risk to human health and ecological environment, making stringent testing of them a crucial step in food safety supervision. Herein, a flexible paper-based SERS platform composited of AgNPs and MoS 2 nanoflowers was developed for the rapid and ultra-sensitive detection of thiram in juice. Among it, the hydrophobic MoS 2 -modified paper was first fabricated, followed by co-depositing small-sized AgNPs and thiram molecules onto its surface for SERS analysis. Owing to the unique petal-like structure and high hydrophobicity of MoS 2 -coated paper, the AgNPs loaded on it was significantly aggregated, thereby generating numerous “hot spots” in the nanogaps between adjacent silver nanoparticles. Simultaneously, thiram molecules were enriched and concentrated within these gaps, resulting in exceptional Raman signal amplification. Compared to single-material substrates, an integrated advantage was acquired with hybridizing of AgNPs and MoS 2 . The performance of this platform was verified by detecting thiram in pear juice using a portable Raman spectrometer, in which the limit of detection (LOD) can be as low as 1.01 ng/mL and its recovery percentages had a good consistency with ones of HPLC-MS/MS. Overall, this Ag/MoS 2 hybrid SERS platform exhibits outstanding stability, sensitivity and feasibility in the label-free detection of pesticides in food. It also provided a universal tool for the on-site monitoring of diverse biochemical molecules across various fields.
研究领域:
分析化学
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纳米技术
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食品安全
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光谱学
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环境科学
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DOI
PubMed
高影响因子
TALANTA
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2025
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IF 6.1
Xin Xu,Ting Ying,Zhanghuan Yang,Ze Zhang,Liang Hu,Tong Shen,Dejian Li,Rui Zhu,Dong Chang
The development of portable exosome detection platforms is crucial for noninvasive early diagnosis of lung cancer, particularly in resource-limited settings. Herein, we engineered a paper-based colorimetric biosensor utilizing a MnO 2 /CeO 2 heterojunction nanozyme for visual quantification of plasma exosomes. The heterojunction synergistically integrates the high surface area of CeO 2 (45.689 m 2 /g) with the nanorod shaped MnO 2 , generating an interfacial electron transfer pathway that enhances peroxidase-like activity by 1.77 and 5.87 fold (K m = 1.197 mM, V max = 0.884 M/min) compared to individual components. Molecular recognition was achieved through dual-target immobilization: exosome-specific aptamers and anti-CD63/FGG/FGB antibodies conjugated on a chitosan-modified cellulose substrate. A smartphone-based hue-saturation-value (HSV) analysis system embedded in the platform enabled rapid on-site screening within 15 min, followed by microplate scanning of suspected high-risk samples, achieving a detection range of 10 2 –10 8 particles/μL with an ultralow detection limit of ∼2 particles/μL. Integration of a logistic regression model incorporating CD63, FGG, and FGB absorbance values further improved classification robustness. Clinical validation using 194 plasma samples demonstrated exceptional diagnostic accuracy in discriminating malignant from benign pulmonary nodules (AUC = 0.996), with a 95 % confidence interval ranging from 0.797 to 1.05, with 98.51 % sensitivity and 95.52 % specificity. This work establishes a multimodal strategy combining heterojunction nanozyme catalysis, paper-based microfluidics, smartphone-assisted imaging, and machine learning, offering a scalable paradigm for liquid biopsy applications in primary healthcare.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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即时检测
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微流控技术
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机器学习
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
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