FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenhui Wang,Zhen-quan Yang,Lixia Xiao,Jie Han,Tianzhu Guan,Xiaojuan Gong,Qin Hu
A paper-based assay for visualization of auramine O (AO) was for the first time established by using CFMs as a ratiometric fluorescent probe (RFP). The CFMs were melamine formaldehyde microspheres (MFMs) incorporated with carbon dots (CDs), where the CDs species as sensing units and MFMs as a signal amplification carrier. The proposed RFP can quantitatively measure AO content from 0.0 to 10.0 μM and exhibited an ultralow limit of detection (LOD, 15.7 nM). In particular, obvious luminescence color change of CFMs from blue to green was perceived with naked-eyes and therefore, a solution-based and a paper-based visualization platform were respectively proposed for on-site visual detection of AO with LODs of 1.15 μM and 0.83 μM, separately. Finally, those fluorescence methods were adopted in sensitively quantitative measurement of AO within various food and drug samples, providing new prospects for analysts and technical support in food quality monitoring.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Tianqi Liu,Xuejuan Wan,Yunsheng Dong,Weibin Li,Lisi Wu,Hang Pei,Youwei Yao
In this study, a facile water-soluble fluorescence sensor 2-((2-hydroxybenzylidene)-amino)-2-(hydroxymethyl)propane-1,3-diol (ST) was synthesized via one-step reaction, and its fluorescence sensing performance for Al 3 + both in aqueous solution and on paper substrate was evaluated. The results showed that ST exhibited an specific fluorescence “turn-on” response to Al 3 + over other cations in aqueous solution as well as on the test paper. The limit of detection was found to be 3.2 × 10 − 7 M, which revealed that the obtained Schiff-base based fluorescence chemosensor ST possessed a great potential for the rapid, quantitative and qualitative detection of Al 3 + .
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 1
·1-10
·
IF 5.3
Zhang Zhikun,Bai Liwei,Han Jilong,Li Zhengjie,Liu Qingju
For the first time a novel fluorescent La@ZrMOF nanomaterial was synthesized for the convenient and visual detection of ethephon (ETH) based on the ligand–metal charge transfer process. The fluorescence signal gradually enhanced as the concentration of ETH increased, accompanied by a change in the color from colorless to blue. The assay can be completed within 75 min with a detection limit of 0.03 mg/L. A paper-based approach for the rapid and visual determination of ETH has also been devised, enabling the efficient used in a variety of actual products, with apples, pears, and tomatoes as examples. It proved to be simple, easy to use, and sensitive, and has the potential for further uses of ETH detection. Graphical
研究领域:
植物生理学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Yuanyuan Ding,Xiaoqian Li,Qingpeng Gao,Xinyan Dong,Liyun Kong,Shengli Han,Tao Zhang,Langchong He
Mas-related G-protein-coupled receptor X2 (MRGPRX2) has recently been reported to be associated with anaphylaxis. Detection of MRGPRX2 levels in human peripheral blood might serve as a powerful tool for predicting the predisposition of patients to anaphylactic reactions. For rapid measurement of MRGPRX2, we established a paper-based double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) using mouse monoclonal antibody and horseradish peroxidase (HRP)-labelled rabbit polyclonal antibody as capture antibody and detection antibody, respectively. We avoided chemical functionalization of the cellulose paper by introducing bovine serum albumin (BSA) to provide COOH and NH 2 groups for covalent immobilization of the capture antibody. Through amide condensation , a two-layer immobilization strategy was applied with BSA-BSA and BSA-capture antibody networks as the first and second layers, respectively. This strategy improved the quantity, activity and stability of the immobilized antibody . We then established a paper-based ELISA to detect MRGPRX2 in human peripheral blood. Our method is less laborious, easier to implement, and more cost-effective than conventional ELISA, while offering similar sensitivity, specificity, and accuracy. Therefore, it could serve as an innovative clinical point-of-care diagnostic tool, especially in areas that lack advanced clinical equipment.
研究领域:
医学
·
免疫学
·
生物化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yuanyuan Zhang,Mei Yang,Zeyu Shao,Hedan Xu,Yan Chen,Yuling Yang,Wenfeng Xu,Xiaoling Liao
A method is described for fluorometric determination of cysteine (Cys) and glutathione (GSH), either in solution by using a fluorescence spectrometer, or by using a paper-based test with visual observation. The method is based on the finding that the yellow fluorescence of the gold/silver nanoclusters (AuAg NCs), with excitation/emission maxima of 358/574 nm, is quenched by Cys and GSH via NCs aggregation. The method is selective for Cys and GSH. The limits of detection for both Cys and GSH are 0.74 nM in solution state and 5.0 μM on paper-based test. Response is linear in the range from 2.3 nM to 110 μM and from 2.3 nM to 63 μM in solution for Cys and GSH, respectively. The method is not interfered by other amino acids even if their concentrations are 10 times higher. It was successfully applied to the determination of Cys in spiked serum samples.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jing Wang,Kai Mu,Hongjuan Wei,Hong Chen,Yunxiang Wang,Wuxing Zhang,Zhen Rong
Acute kidney injury (AKI) is a serious renal emergency that is often underdiagnosed and associated with high mortality rates. Early diagnosis of AKI is crucial to improve patient prognosis and reduce healthcare costs. In this study, a paper-based colloidal gold multiplex vertical flow assay was developed for rapid detection of three AKI biomarkers, namely, NGAL , CysC, and RBP4 , in a single test kit. In this system, specific capture antibodies were pre-immobilized on the sensing membrane at four spatially isolated immunoreactive sites. Gold nanoparticles were conjugated with detection antibodies as the colorimetrical labels. The resultant images could be read out qualitatively by the naked eyes or quantitatively by a smartphone-based readout device. Three AKI biomarkers could be simultaneously detected in 10–15 min. The detection sensitivity and specificity for clinical serum samples collected from 25 patients with AKI and 25 healthy individuals were determined as 92% and 88%. This vertical flow assay platform is easy to use, rapid, and low cost. It is a promising diagnostic tool for multiplex detection of disease biomarkers at point of care.
研究领域:
医学诊断
·
生物传感
·
肾脏病学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Lanlan Xu,Bingyu Yang,Liangqia Guo
Herein, oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB) nanobelts were developed to enhance the colorimetric and paper-based sensing of H 2 O 2 . It was found that the minor component of Fe 2+ in Na 2 SO 4 reagent could catalyze the oxidization of TMB by H 2 O 2 into positively charged oxTMB, which was further assembled into dark blue oxTMB nanobelts via electrostatic interaction with SO 4 2− . The extinction originating from the absorption and scattering of oxTMB nanobelts was utilized to quantitatively detect H 2 O 2 with a wide linear detection range (1.0–300 μM) and a low limit of detection (0.48 μM). In addition, no coffee-ring effect was observed in the test zone of the paper-based colorimetric array, which was beneficial to judge the color by naked eye. Finally, the colorimetric method was applied to detect H 2 O 2 in contact lens care solution. This work not only proposed a new colorimetric sensing platform for H 2 O 2 , but also highlighted the minor component in the reagent might influence the experimental result.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 13
·3541-3550
·
IF 4.157
Li Zimu,Xi Yachao,Zhao Anqi,Jiang Jianming,Li Bing,Yang Xinming,He Jianbo,Li Fang
Chemiluminescence (CL) reagent luminol was loaded into the porous structure of cobalt-imidazole metal-organic framework (MOF) ZIF-67 to obtain luminol-functionalized ZIF-67 (luminol@ZIF-67) with CL property. The morphology, composition, CL property, and CL mechanism of luminol@ZIF-67 were carefully investigated. The obtained luminol@ZIF-67 exhibited strong, stable, and visible CL emission that reacted with H 2 O 2 , attributed to the strong catalytic effect of ZIF-67 combined with the shortened diffusion distance between luminol and the catalytic center. The CL intensity of luminol@ZIF-67 was more than 550 times higher than that of luminol. Catechol can effectively quench the CL emission of luminol@ZIF-67 that reacted with H 2 O 2 . Then, a simple paper-based CL imaging detection method was developed for the detection of catechol by using a smartphone as a portable detector. The linear calibration curve of the developed CL assay for catechol ranged from 5 to 100 mg/L with detection limit of 1.1 mg/L (S/N = 3δ). The strong CL emission of luminol@ZIF-67 combined with the effective quench ability of catechol guaranteed high sensitivity of the detection method. The practical application ability of the developed CL assay was tested by the determination of catechol in tea and tap water samples, resulting in acceptable results. This work provides an effective paper-based CL detection method for catechol and enriches the species of the chemiluminescent MOF material. Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Liu Tong,Lina Wu,Yunfeng Zai,Yu Zhang,Enben Su,Ning Gu
A novel paper-based colorimetric glucose sensor was proposed employing Prussian blue nanoparticles (PB NPs) as mimic peroxidase . The sensor was manufactured by spraying solution containing PB NPs, glucose oxidase and chromogenic agents into a paper, then coating the filter layer and spreading layer on the top. The layer-by-layer structure enabled the sensor detect glucose in whole blood, as well as elimination of the coffee-ring effect which ensure the performance. As a powerful alternative to natural peroxidase, PB NPs showed the mimic enzymatic activity well preserved in dry environment. The manufacture process of the sensor is easy to be industrialized. Under optimal conditions, the sensor exhibited a linear range from 2.5 mM to 25 mM for glucose in blood with satisfactory reproducibility (the coefficient of variant <4%), great storage stability (1 month at 45 °C) and excellent linearity compared with those commercial kits (R > 0.99). Coupled with a handhold device, the PB NPs-based test strip realized the goal of personal operation, user-friendly control, automatic readouts, and data storage, and able to link the Cloud, showing unique potential in clinical application, especially in community-level medical scenarios.
研究领域:
生物化学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2021
·
IF 5.565
Yanan Zheng,Liudan Wei,Linwei Duan,Fangfang Yang,Guixiang Huang,Tianyi Xiao,Min Wei,Yanling Liang,Huiting Yang,Zhipeng Li,Dan Wang
With increasing industrial activities, mercury has been largely discharged into environment and caused serious environmental problems. The growing level of mercury pollution has become a huge threat to human health due to its significant biotoxicity. Therefore, the simple and fast means for on-site monitoring discharged mercury pollution are highly necessary to protect human beings from its pernicious effects in time. Herein, a “turn off” fluorescent biosensor (mCherry L199C) for sensing Hg 2+ was successfully designed based on direct modification of the chromophore environment of fluorescent protein mCherry. For rapid screening and characterization, the designed variant of mCherry (mCherry L199C) was directly expressed on outer-membrane of Escherichia coli cells by cell surface display technique. The fluorescent biosensor was characterized to have favorable response to Hg 2+ at micromole level among other metal ions and over a broad pH range. Further, the cells of the fluorescent biosensor were encapsulated in alginate hydrogel to develop the cells-alginate hydrogel-based paper. The cells-alginate hydrogel-based paper could detect mercury pollution in 5 min with simple operation process and inexpensive equipment, and it could keep fluorescence and activity stable at 4 °C for 24 hr, which would be a high-throughput screening tool in preliminarily reporting the presence of mercury pollution in natural setting.
研究领域:
环境科学
·
生物技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-11
·
IF 6.232
Li Fang,Hu Yuting,Zhao Anqi,Xi Yachao,Li Zimu,He Jianbao
β -cyclodextrin-functionalized porous
Pd@Au nanostructures ( β -CD-Pd@Au) with intrinsic
and enhanced peroxidase-like activity were successfully synthesized by a two-step
method. The synthesized β -CD-Pd@Au can
efficiently catalyze the oxidation of various substrates, such as
3,3′,5,5′-tetramethylbenzidine (TMB), mixture of 4-amino antipyrine (4-AAP) and
3,5-dichloro-2-hydroxy acid sodium (DHBS) (4-AAP/DHBS), and mixture of 4-AAP and
N-Ethyl-N-(3-sulfopropyl)-3-methyl-aniline sodium salt (TOPS) (4-AAP/TOPS), by
H 2 O 2 to generate visual blue,
purple, and pink color, respectively. The UV-vis absorbance peak of the three β -CD-Pd@Au catalyzed the chromogenic reaction
system located at 650 nm, 510 nm, and 550 nm, respectively. The β -CD-Pd@Au-catalyzed
TMB-H 2 O 2 chromogenic reaction
exhibited higher absorbance intensity, catalytic efficiency, and color stability in
comparison to 4-AAP/DHBS-H 2 O 2 and
4-AAP/TOPS-H 2 O 2 chromogenic
reactions. The catalytic activity of β -CD-Pd@Au
was enhanced about 4-fold compared to that of Pd@Au in terms of
K cat for
H 2 O 2 . Using TMB as chromogenic
substrate, a colorimetric assay was fabricated for the determination of
H 2 O 2 with a detection limit of
2.78 μM (absorbance at 650 nm). The colorimetric determination of glucose with a
detection limit of 9.28 μM was further achieved by coupling with glucose oxidase
enzymatic reaction, indicating the versatility of the β -CD-Pd@Au-based detection strategy. A paper-based detection method
coupled with smartphone for fast visual and instrument-free detection of glucose was
further developed. Finally, the developed colorimetric assay and paper-based
detection method were successfully applied to the determination of glucose in human
serum sample.
Graphical abstract
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Translational Medicine
·
2017
·Vol. 9
·期 381
·
IF 16.761
Hong Zhang,Xiaopei Qiu,Yurui Zou,Yanyao Ye,Chao Qi,Lingyun Zou,Xiang Yang,Ke Yang,Yuanfeng Zhu,Yongjun Yang,Yang Zhou,Yang Luo
Fast and simultaneous forward and reverse blood grouping has long remained elusive. Forward blood grouping detects antigens on red blood cells, whereas reverse grouping identifies specific antibodies present in plasma. We developed a paper-based assay using immobilized antibodies and bromocresol green dye for rapid and reliable blood grouping, where dye-assisted color changes corresponding to distinct blood components provide a visual readout. ABO antigens and five major Rhesus antigens could be detected within 30 s, and simultaneous forward and reverse ABO blood grouping using small volumes (100 μl) of whole blood was achieved within 2 min through on-chip plasma separation without centrifugation. A machine-learning method was developed to classify the spectral plots corresponding to dye-based color changes, which enabled reproducible automatic grouping. Using optimized operating parameters, the dye-assisted paper assay exhibited comparable accuracy and reproducibility to the classical gel-card assays in grouping 3550 human blood samples. When translated to the assembly line and low-cost manufacturing, the proposed approach may be developed into a cost-effective and robust universal blood-grouping platform.
研究领域:
医学诊断
·
即时检测技术
·
生物化学
·
医学中的机器学习
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）