Xiaoyan Tang,Luying Chen,Yang Ding,Huanping Liu,Muzi Li,Yang Yang
Both nanoplastics (NPs) and imidacloprid (IMI) are widely distributed in the environment and have attracted significant attention due to their adverse effects on ecosystems. Constructed wetlands have the potential to remove IMI, but there is still limited understanding of how wetland plants interact with IMI, especially when influenced by different charged NPs. This study assessed their ecotoxicological effects, as well as the fate and transformation of IMI in papyrus ( Cyperus papyrus L.) under the influence of different charged NPs and identified key driving genes in the plant. Results show that simultaneous exposure to positively charged PS-NH 2 and IMI inhibited plant growth. The combined action of NPs and IMI intensified their toxicity, enhancing lipid peroxidation and altering antioxidant enzyme activities . The IMI removal efficiency, which was primarily driven by biodegradation, was 80.61%, 88.91%, and 74.71% in the IMI-alone, co-IMI/PS_COOH, and co-IMI/PS_NH 2 systems, respectively. PS-NH 2 restricted the roots-to-shoots translocation ability of IMI. PS-COOH enhanced IMI oxidation and nitro reduction, while PS-NH 2 inhibited 2-OH-IMI dehydrogenation to IMI-olefin in papyrus. Transcriptomics and gene network analysis identified the genes encoding CYP450 enzymes, reductases, hydrolases, dehydrogenases , and peroxidases as those influencing IMI biodegradation. These enzymes play a crucial role in the hydroxylation, dehydrogenation , reduction, and oxidation processes during biodegradation of IMI in the presence of NPs. This study expands the understanding of the impact of differently charged NPs on the IMI remediation efficacy of papyrus, thus providing new insights into the phytoremediation of organic contaminants in constructed wetlands.
Huanping Liu,Xiaoyan Tang,Xiaomin Xu,Yunv Dai,Xiaomeng Zhang,Yang Yang
Broad-spectrum insecticides such as neonicotinoids tend to accumulate and detrimentally impact natural ecosystems. Accordingly, we aimed to assess the neonicotinoid phytoremediation abilities of nine wetland plant species commonly used in constructed wetland systems: Acorus calamus , Typha orientalis , Arundo donax , Thalia dealbata , Canna indica , Iris pseudacorus , Cyperus alternifolius , Cyperus papyrus and Juncus effusus. We assessed their removal of six neonicotinoids and explored the mechanisms responsible for the observed removal in a 28-day experiment. The planted systems effectively removed the neonicotinoids, with removal efficiencies of 9.5–99.9%. Compared with the other neonicotinoids, imidacloprid , thiacloprid and acetamiprid were most readily removed in the planted systems. C. alternifolius and C. papyrus exhibited the best removal performance for all six neonicotinoids. Based on our assessment of mass balance, the main removal processes were biodegradation and plant accumulation. Plants can enhance neonicotinoid removal through enhancing biodegradation. The differences in transport and accumulation behaviors may be related to plant species and physicochemical properties of neonicotinoids. Further research is merited on the toxicity of neonicotinoids to plants and microorganisms and the metabolic pathways by which neonicotinoids are broken down in wetland systems.
危险品化学品经营许可证（带存储）