PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Wenwen Song,Mingming Dai,Shasha Gao,Yindong Mi,Shijia Zhang,Jianyong Wei,Honghai Zhao,Fangmeng Duan,Chen Liang,Qianqian Shi
Background Root knot nematodes cause great damage to crops worldwide. Due to the negative effects of the application of fumigant and old chemical nematicides, biological nematicides have drawn increasing attention in recent years. Here we tested the fumigant activity of the volatile organic compounds (VOCs) blends emitted from Paenibacillus polymyxa and pure commercial VOCs against M. incognita . Results In this study, we investigated whether P. polymyxa strain J2-4 could produce VOCs that exhibit nematicidal activity. In vitro assays indicated that J2-4 VOCs were highly toxic to second stage juveniles (J2s) and could inhibit egg hatching. Three-layered pot experiments showed that the number of nematodes that penetrating in cucumber roots was reduced by 69.27% after the application of J2-4 VOCs under greenhouse conditions. We identified fourteen volatiles using solid-phase micro-extraction gas chromatography–mass spectrometry. The efficacy of six commercially available VOCs, namely 2-isobutyl-3-methylpyrazine, 2,4-dimethoxybenzaldoxime, 2-dodecanone, 2-tridecanol, 2-tridecanone, and 2-tetradecanol, against M. incognita were examined. Except for 2,4-dimethoxybenzaldoxime, the remaining five VOCs showed strong direct-contact nematicidal activity against J2s of M. incognita , and only 2-isobutyl-3-methylpyrazine showed strong fumigant activity against J2s of M. incognita . In pot experiments, 2-isobutyl-3-methylpyrazine and 2-dodecanone reduced the number of root galls by about 70%, and 2-tridecanone reduced the number of root galls and egg masses by about 63% compared with controls. Conclusion Paenibacillus polymyxa strain J2-4 exhibited high fumigant activity against M. incognita. Our results provide evidence for the use of J2-4 and its VOCs as biocontrol agents in the management of root-knot nematodes. This article is protected by copyright. All rights reserved.
研究领域:
植物病理学
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农业科学
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微生物学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Qiyi Zhu,Xiaofeng Zhu,Ning Yang,Yuxi Duan,Yuanyuan Wang,Xiaoyu Liu,Di Zhao,Haiyan Fan,Lijie Chen
Root-knot nematodes ( Meloidogyne incognita ) present a significant threat to global agriculture, and the development of multi-drug resistance in these nematodes exacerbates this problem. Benzothiazole, a heterocyclic compound has been reported as a potential nematicide, however, its mode of action is not fully understood. This study aims to elucidate the nematicidal mechanism of benzothiazole against M. incognita . In the toxicity assays, benzothiazole exhibited rapid and effective nematocidal activity, significantly compromising egg masses and inhibiting egg hatching while killing newly hatched second-stage juveniles (J2s) of M. incognita . Microscopic observations revealed that after 48 h of incubation, a marked reduction in protein and carbohydrate levels within the J2s was observed. Notably, benzothiazole at a concentration of 14 mmol/L significantly inhibits glutathione S-transferase (GST) enzyme activity, leading to the accumulation of reactive oxygen species (ROS), ultimately resulting in rapid nematode death. Molecular docking and dynamics simulations demonstrated that benzothiazole forms a stable complex with GST, thereby disrupting its antioxidant function. Furthermore, in pot experiments, benzothiazole effectively reduced the gall formations of M. incognita on tomato roots. Overall, this novel inhibitory mechanism of glutathione S-transferase (GST) differs from that of the neurotoxicant abamectin, which targets glutamate-gated chloride channels (GluCl). This mechanism holds significant promise for the development of environmentally friendly nematicides. It offers a potential solution to the growing problem of multidrug resistance in root-knot nematodes and could help mitigate the substantial economic losses caused by these pests in global agriculture.
研究领域:
农业科学
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害虫防治
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生物化学
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线虫学
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植物病理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 4
·2080
·
IF 6.208
Zhaoqian Fan,Yukun Qin,Song Liu,Ronge Xing,Huahua Yu,Kecheng Li,Pengcheng Li
The exploration of novel, environmentally friendly, and efficient nematicides is essential, and modifying natural biomacromolecules is one feasible approach. In this study, 6-O-(trifluorobutenyl-oxadiazol)-chitosan oligosaccharide derivative was synthesized and characterized by FTIR, NMR, and TG/DTG. Its bioactivity and action mode against root-knot nematodeM. incognitawere estimated. The results show that the derivative shows high nematicidal activity against J2s, and egg hatching inhibitory activity at 1 mg/mL. The derivative may affect nematode ROS metabolism and further damage intestinal tissue to kill nematode. Meanwhile, by synergism with improving crop resistance, the derivative performed a high control effect on the nematode with low phytotoxicity. These findings suggested that chitosan oligosaccharide derivatives bearing fluoroalkenyl groups are promising green nematicides.Keywords:chitosan oligosaccharide;nematicide;Meloidogyne incognita;trifluorobutenyl group
研究领域:
农业化学
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农药学
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植物病理学
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DOI
PubMed
高影响因子
JOURNAL OF PEST SCIENCE
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2025
·Vol. 99
·期 1
·2
·
IF 4.1
Zhao Zhijiao,Zhu Xiaofeng,Wang Yuanyuan,Liu Xiaoyu,Yang Ning,Fan Haiyan,Chen Lijie,Sikanda Aatika,Duan Yuxi
Root-knot nematodes (RKNs, especially Meloidogyne incognita ) are a growing threat to greenhouse tomatoes in China. However, control methods in the greenhouses in China are limited. The use of chemical pesticides such as fluopyram and thiazole phosphate to control M. incognit a is expensive and harmful to the environment. The Chinese herb Asarum sieboldii is commonly used, and it shows potential as a plant-derived pesticide. We evaluated the effectiveness of A. sieboldii in regulating the soil nematode community and M. incognita in tomato greenhouses. Combining morphological identification with high-throughput sequencing techniques, we demonstrated that A. sieboldii root extract significantly reduced the relative abundance of plant-parasitic nematodes (PPNs) while promoting the ecological and functional recovery of bacterivorous (BF) and fungivorous (FF) nematodes. The aqueous extract exhibited high toxicity against M. incognita J2s and reduced the number of root galls by 69.5% per gram of root, decreased the number of egg masses by 58.4%, and enhanced shoot fresh weight by 53.6%. Through column chromatography and HPLC–MS analysis, mono-ethyl fumarate (MEF) and methyl gallate (MG) were identified as key active compounds, demonstrating LC50 values of 10.20 mg/L and 49.47 mg/L with root-knot suppression rates reaching 64.8% and 61.3% under greenhouse conditions. This study presents the first evidence that A. sieboldii aqueous extract effectively controls M. incognita without compromising the ecological balance of soil, providing a theoretical foundation for developing environmentally friendly botanical nematicides.
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高影响因子
MOLECULES
·
2017
·Vol. 22
·期 5
·798
·
IF 2.861
Jia Wang,Guangzhi Zeng,Xiaobing Huang,Zhe Wang,Ninghua Tan
Plant-parasitic nematodes are destructive pathogens causing enormous economic losses worldwide. With the withdrawal of fumigants, organophosphates and carbamates, pathogenic nematode control is more difficult. Phytochemicals are the plant secondary metabolites and are friendly for men and the environment. For developing new nematocidal candidates, we screened 790 phytochemicals using the model organismCaenorhabditis elegansand found 10 active compounds, 3 of which were further evaluated for their inhibitory activities against egg hatching ofC. elegansand J2Meloidogyne incognita.Among them, 1,4-naphthoquinone (1,4-NQ) was the only compound that could kill more than 50% of targets at 50 μg/mL, prompting us to investigate how 1,4-NQ triggers nematode lethality. InC. elegans, we observed that 1,4-NQ could influence reactive oxygen production, superoxide dismutase activity, and the heat-shock transcription factor (HSF)-1 pathway, which indicated that 1,4-NQ stimulated significant oxidative stress. Furthermore, using quantitative RT-PCR and transgenetic nematodes, we revealed that 1,4-NQ lethality was related to the Insulin/IGF signaling (IIS) pathway, and the effect of 1,4-NQ on IIS pathway related genes indicated that 1,4-NQ could activate this pathway and suppress the expression of DAF-16 target genes. The triggering of oxidative stress and activation of the IIS pathway indicated that 1,4-NQ operates through the generation of oxygen radicals, which can be lethal toC. elegans, thus making it an interesting lead compound for the development of future nematocides.Keywords:plant-parasitic nematode;phytochemical;nematocidal;1,4-naphthoquinone;oxidative stress;insulin/IGF signaling pathway;Caenorhabditis elegans
研究领域:
植物科学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
MOLECULAR DIVERSITY
·
2024
·
IF 3.9
Ou Yuqin,Guo Xue,Zhang Qi,Zhang Wei,Gan Xiuhai
Plant-parasitic nematodes are seriously affecting agricultural production worldwide and there are few highly effective and low-risk nematicides to control nematode diseases. In order to discover new nematicides, a series of 1,2,4-oxadiazole derivatives containing amide fragments have been designed and synthesized with the principle of active substructure splicing. The nematicidal activity of the target compounds was evaluated in vitro and it indicated that compound C3 exhibited the most nematicidal activity against Bursaphelenchus xylophilus , Aphelenchoides bessey i, and Ditylenchus destructor with the LC 50 values of 37.2, 36.6, and 43.4 μg/mL, respectively, which were superior to positive agent tioxazafen. The preliminary mechanism results revealed that compound C3 not only inhibited the reproduction of B. xylophilus populations, but also affected the production of ROS and the accumulation of lipofuscin and lipids. Furthermore, compound C3 showed good inhibition of succinate dehydrogenase (SDH) with the IC 50 value of 45.5 µmol/L. Molecular docking indicated that compound C3 had excellent binding to amino acids around the SDH active pocket. This work indicated that 1,2,4-oxadiazole derivative containing amide fragment is a promising template for the discovery of new nematicides and compound C3 can be used as a potential nematicide candidate. Graphical abstract
研究领域:
农业化学
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农药科学
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分子生物学
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DOI
PubMed
高影响因子
ARCHIVES OF MICROBIOLOGY
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2022
·Vol. 204
·期 8
·1-10
·
IF 2.667
Ye Lin,Wang Jian-Yu,Liu Xiao-Fang,Guan Qi,Dou Nong-Xiao,Li Jian,Zhang Qian,Gao Yan-Ming,Wang Min,Li Jian-She,Zhou Bo
The application of nematicidal microorganisms and their virulence factors provides more opportunities to control root-knot nematodes. Bacillus altitudinis AMCC 1040, previously isolated from suppressive soils, showed significant nematicidal activity, and in this study, nematicidal substances produced by Bacillus altitudinis AMCC 1040 were investigated. The results of the basic properties of active substances showed that these compounds have good thermal stability and passage, are resistant to acidic environment and sensitive to alkaline conditions. Further analysis showed that it is a volatile component. Using HS-SPME-GC/MS, the volatile compounds produced by Bacillus altitudinis AMCC 1040 were identified and grouped into four major categories: ethers, alcohols, ketone, and organic acids, comprising a total of eight molecules. Six of them possess nematicidal activities, including 2,3-butanedione, acetic acid, 2-isopropoxy ethylamine, 3-methylbutyric acid, 2-methylbutyric acid and octanoic acid. Our results further our understanding of the effects of Bacillus altitudinis and its nematicidal metabolites on the management of Meloidogyne incognita and may help in finding less toxic nematicides to control root knot nematodes.
研究领域:
植物病理学
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微生物学
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DOI
PubMed
高影响因子
Agriculture-Basel
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2024
·Vol. 14
·期 2
·261
·
IF 3.3
Shiva Parsiaaref,Aocheng Cao,Yuan Li,Asgar Ebadollahi,Ghasem Parmoon,Qiuxia Wang,Dongdong Yan,Wensheng Fang,Bin Huang,Min Zhang
Meloidogynespp. are important parasitic nematodes affecting a variety of plants worldwide. We investigated the nematicidal properties of specific compounds found in bio-fumigant plants, particularly linalool, nonanal, methylene chloride, and 2-Decanal. Laboratory findings revealed that methylene chloride and 2-Decenal effectively reduced populations of second-stage juveniles (J2s). Additionally, the research explored the effects of tomato (Solanum lycopersicumL.) onM. incognitaJ2s, observing that tomato leaves significantly increased J2 mortality for all time measurements and different temperatures, while the opposite results were observed for root-stems. In the study, leaf treatment resulted in a maximum mortality response (MRmax) and half-maximal effective concentration (EC50) of approximately 100% and 4.0 µg/mg, respectively, at a temperature of 35 °C by week 8. In contrast, the root-stems treatment showed an MRmaxof 13.5% and an EC50of 3.0 ± 1.7 µg/mg. GC-MS analysis identified key compounds in tomato leaves and root-stems, such as α-pinene, d-limonene, and linalool. The results suggest that tomato leaves have potential as effective bio-fumigants for controlling root-knot nematodes.
研究领域:
植物科学
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线虫学
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农业化学
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害虫管理
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DOI
高影响因子
Jove-Journal of Visualized Experiments
·
2022
·
IF 1.424
Xiaofang Liu,Xiang Zhou,Lifeng Zhou,Jiafu Hu,Kai Guo
The pinewood nematode, Bursaphelenchus xylophilus, is one of the most destructive invasive species worldwide, causing the wilting and eventual death of pine trees. Despite the recognition of their economic and environmental significance, it has thus far been impossible to study the detailed gene functions of plant-parasitic nematodes (PPNs) using conventional forward genetics and transgenic methods. However, as a reverse genetics technology, RNA interference (RNAi) facilitates the study of the functional genes of nematodes, including B. xylophilus. This paper outlines a new protocol for RNAi of the ppm-1 gene in B. xylophilus, which has been reported to play crucial roles in the development and reproduction of other pathogenic nematodes. For RNAi, the T7 promoter was linked to the 5′-terminal of the target fragment by polymerase chain reaction (PCR), and double-stranded RNA (dsRNA) was synthesized by in vitro transcription. Subsequently, dsRNA delivery was accomplished by soaking the nematodes in a dsRNA solution mixed with synthetic neurostimulants. Synchronized juveniles of B. xylophilus (approximately 20,000 individuals) were washed and soaked in dsRNA (0.8 µg/mL) in the soaking buffer for 24 h in the dark at 25 °C.The same quantity of nematodes was placed in a soaking buffer without dsRNA as a control. Meanwhile, another identical quantity of nematodes was placed in a soaking buffer with green fluorescent protein (gfp) gene dsRNA as a control. After soaking, the expression level of the target transcripts was determined using real-time quantitative PCR. The effects of RNAi were then confirmed using microscopic observation of the phenotypes and a comparison of the body size of the adults among the groups. The current protocol can help advance research to better understand the functions of the genes of B. xylophilus and other parasitic nematodes toward developing control strategies through genetic engineering.
研究领域:
分子生物学
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植物病理学
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线虫学
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遗传学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 3
·1596
·
IF 6.208
Dan Liu,Ling Luo,Zhengxing Wang,Xiaoyun Ma,Xiuhai Gan
Plant diseases that are caused by fungi and nematodes have become increasingly serious in recent years. However, there are few pesticide chemicals that can be used for the joint control of fungi and nematodes on the market. To solve this problem, a series of novel 1,2,4-oxadiazole derivatives containing amide fragments were designed and synthesized. Additionally, the bioassays revealed that the compoundF15demonstrated excellent antifungal activity againstSclerotinia sclerotiorum(S. sclerotiorum) in vitro, and the EC50value of that was 2.9 μg/mL, which is comparable with commonly used fungicides thifluzamide and fluopyram. Meanwhile,F15demonstrated excellent curative and protective activity againstS. sclerotiorum-infected cole in vivo. The scanning electron microscopy results showed that the hyphae ofS. sclerotiorumtreated withF15became abnormally collapsed and shriveled, thereby inhibiting the growth of the hyphae. Furthermore,F15exhibited favorable inhibition against the succinate dehydrogenase (SDH) of theS. sclerotiorum(IC50= 12.5 μg/mL), and the combination mode and binding ability between compoundF15and SDH were confirmed by molecular docking. In addition, compoundF11showed excellent nematicidal activity againstMeloidogyne incognitaat 200 μg/mL, the corrected mortality rate was 93.2%, which is higher than that of tioxazafen.Keywords:1,2,4-oxadiazole;antifungal activity;nematicidal activity;succinate dehydrogenase
研究领域:
农业化学
·
植物病理学
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农药科学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 5
·2205
·
IF 4.6
Shengli Shi,Xinsha Zhang,Xianbin Liu,Zhen Chen,Hewen Tang,Dongbao Hu,Hongmei Li
The essential oil (EO) was extracted from aerial parts with insecticidal and fungicidal activity. Herein, the hydro-distilled essential oils ofSeseli maireiH. Wolff roots were determined by GC-MS. A total of 37 components were identified, (E)-beta-caryophyllene (10.49%),β-geranylgeranyl (6.64%), (E)-2-decenal (6.17%) and germacrene-D (4.28%). The essential oil ofSeseli maireiH. Wolff had nematicidal toxicity againstBursaphelenchus xylophiluswith a LC50value of 53.45 μg/mL. The subsequent bioassay-guided investigation led to the isolation of three active constituents: falcarinol, (E)-2-decenal, and octanoic acid. The falcarinol demonstrated the strongest toxicity againstB. Xylophilus(LC50= 8.52 μg/mL). The octanoic acid and (E)-2-decenal also exhibited moderate toxicity againstB. xylophilus(LC50= 65.56 and 176.34 μg/mL, respectively). The LC50of falcarinol for the toxicity ofB. xylophiluswas 7.7 and 21 times than that of octanoic acid and (E)-2-decenal, respectively. Our findings demonstrate that the essential oil fromSeseli maireiH. Wolff roots and their isolates may be developed as a promising natural nematicide.Keywords:Seseli maireiH. Wolff;Bursaphelenchus xylophilus;falcarinol
研究领域:
植物化学
·
植物保护
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药理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 18
·9668
·
IF 5.924
Xiaoxuan Yu,Hui Li,Dongfa Lin,Weizhuo Guo,Zhihao Xu,Liping Wang,Shuwen Guan
Panax ginsengis a valuable traditional Chinese medicine in Northeast China. Ginsenoside, the active component of ginseng, has not been investigated much for its effects on aging and its underlying mechanism(s) of action. Here, we investigated the effects of total ginsenoside (TG), a mixture of the primary active ginsenosides fromPanax ginseng, on the lifespan ofCaenorhabditis elegans(C. elegans). We found that TG extended the lifespan ofC. elegansand reduced lipofuscin accumulation. Moreover, TG increased the survival ofC. elegansin response to heat and oxidative stress via the reduction of ROS. Next, we used RNA-seq to fully define the antiaging mechanism(s) of TG. The KEGG pathway analysis showed that TG can prolong the lifespan and is involved in the longevity regulating pathway. qPCR showed that TG upregulated the expression ofnrh-80,daf-12,daf-16,hsf-1and their downstream genes. TG also reduced the fat accumulation and promoted lipid metabolism. Moreover, TG failed to extend the lifespan ofdaf-16andhsf-1mutants, highlighting their role in the antiaging effects of TG inC. elegans. The four main constitution of TG were then confirmed by HPLC and included ginsenoside Re, Rg1, Rg2and Rd. Of the ginsenosides, only ginsenoside Rd prolonged the lifespan ofC. elegansto levels comparable to TG. These findings provided mechanistic insight into the antiaging effects of ginsenoside inC. elegans.Keywords:Panax ginseng;lifespan;lipid metabolism;stress-resistant;Caenorhabditis elegans
研究领域:
药理学
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衰老研究
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分子生物学
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DOI
PubMed
高影响因子
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