JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
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2012
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IF 2.625
Chi Ma,Di Pang,Zuochun Xiong,Wei Bai,Chengdong Xiong
Control of cellular responses is crucial for the use of electrospun membranes in biomedical applications, including tissue engineering or biomedical devices. However, it is still unclear whether adhesion and proliferation of fibroblasts is stimulated or inhibited on polyethylene glycol (PEG)-modified electrospun membranes. In this study, poly( L -lactide-co-glycolide) (PLLGA)–PEG copolymer and pure PEG were blended with PLLGA, and then electrospun onto nonwoven membranes. The effects of blending of PLLGA–PEG or pure PEG on the adsorption of proteins, and further on the adhesion and proliferation of L929 fibroblasts on the electrospun membranes were investigated. Addition of PLLGA–PEG or PEG significantly improved the hydrophilicity of the electrospun membranes. Pure PEG had no obvious effects on the growth of L929 fibroblasts; in contrast, PLLGA–PEG significantly inhibited the adsorption of proteins and the proliferations of the cells on the electrospun membranes. In response to diminished protein adsorption, mRNA expression of genes related to cell adhesion and migration was up-regulated. The limited effects of pure PEG were probably caused by its preferential dissolution, whereas membrane-confined PLLGA–PEG displayed excellent performance on the inhibition of protein adsorption and cell proliferation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2897–2904, 2012.
研究领域:
生物医学工程
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组织工程
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DOI
PubMed
高影响因子
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
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2025
·
IF 2.4
Tong Zhang,Bin Xu
Gastric cancer (GC) is a malignant tumor with high morbidity and mortality rates worldwide. This study aimed to investigate the effects and mechanisms of action of didymin, a dietary flavonoid glycoside, on GC treatment. Human GC cell lines Hs-746T and AGS were used to assess the effects of didymin on cell viability, cell proliferation, and cell cycle. The results showed that didymin decreased the proliferative capacity of GC cells and blocked cell cycle. Didymin decreased wound healing, invasion, and migration capacities of GC cells. Mitochondrial reactive oxygen species (ROS) levels and mitochondrial membrane potentials were reduced in cells treated with didymin. Network pharmacology analysis revealed that the therapeutic effects of didymin on AGS cells were related to the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. In vivo mouse xenograft studies confirmed that didymin treatment decreased tumor cell proliferation, cell cycle protein levels, and Akt phosphorylation. The present study demonstrated that didymin regulates mitochondrial function and the PI3K/Akt pathway to inhibit cell proliferation and induce apoptosis in GC cells in vitro and in vivo. Therefore, didymin is a promising drug for the treatment of GC.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
PROSTAGLANDINS & OTHER LIPID MEDIATORS
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2025
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IF 2.5
Jindao Wang,Minghui Zhou,Qiuli Zhou,Guangyang Sun,Yu Zhang,Feng Tao,Minfeng Ye
Background Gastric cancer (GC) is the third leading culprit of cancer-related deaths around the world. Beta-sitosterol (BS) is an important phytosterol that has been proven to have anti-proliferative effects on GC and other tumors. However, mechanisms and targets of BS in cancer are rarely explored. Methods In this investigation, the targets of BS in the treatment of GC were analyzed by network pharmacology. Molecular docking and cellular thermal shift assay were introduced to validate the binding relationship between BS and PTGS1. The impacts of BS on GC cell viability, half maximal inhibitory concentration, proliferation ability, apoptosis level, and angiogenesis ability were detected by using Cell Counting Kit-8, clone formation assay, flow cytometry, and angiogenesis experiment, respectively. In addition, the expression levels of angiogenic factors (VEGF, FGF, PAI-1) were detected by using western blot. Results In this project, through cell experiments, PTGS1 was identified as a protein that directly binds to BS. In vitro cell experiments revealed that BS promoted apoptosis and inhibited GC cell proliferation and angiogenesis. Importantly, treatment with BS attenuated the promoting influence of PTGS1 overexpression on GC cell proliferation and angiogenesis. Conclusion This investigation highlighted PTGS1 as the target of BS in GC cells. BS can regulate PTGS1 to inhibit GC cell proliferation and angiogenesis, providing new evidence for the potential use of BS as a therapeutic agent for GC.
研究领域:
肿瘤学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER ENGINEERING
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2024
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IF 1.7
Yan Ren,Yonggang Lin,Yabei Ding,Ruimin Tang,Minglong Yuan,Hongli Li,Yongming Chuan
Poly( l -lactide- co -caprolactone) (PLCL) is the polymeric material with good biodegradability and biocompatible properties, but it is insufficient in strength properties that the pure PLCL is performed as orthopedic bandage. The PLCL has been blended with the laponite which is modified by cetyltrimethylammonium bromide (CTAB) to prepare composited bandage splines. The organic-modified laponite (OM-laponite) results of Fourier transform infrared (FTIR) and thermogravimetry analysis (TG) indicate that the laponite was successfully grafted with CTAB and the thermal stability was improved with increasing CTAB. The mechanical properties reveal that elongation at break strengthen with the increasing OM-laponite. When the content of OM-laponite is 2.0 %, the elongation at break and tensile strength of composited spline are superior to the medical orthopedic bandage. The results of thermal stability show that it was helpful to improve the crystallization properties of PLCL with the addition of OM-laponite. The water contact angle results demonstrate that the hydrophilicity is enhanced and is beneficial to cell adhesion. The results of cell experiment illustrate that the composite materials have a certain effect on cell proliferation when the content of OM-laponite is less than 2 %. Compared with the medical orthopedic bandage, it is satisfied with the application requirements.
研究领域:
生物医学工程
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF CLINICAL LABORATORY ANALYSIS
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2022
·Vol. 36
·期 11
·e24698
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IF 3.124
Chunfa Chen,Liang Liu
Background Osteosarcoma (OS) is a highly recurrent malignancy occurring among adolescents. The goal of this research was to scrutinize the role and action mechanism of KLF3-AS1 in OS. Methods Western blotting and quantitative reverse transcription real-time PCR were conducted to ascertain the mRNA expressions of miR-338-3p, KLF3-AS1, and MEF2C in OS cell lines and tissue samples. Colony formation and CCK-8 experiments were done to evaluate the proliferative capacity of the cells. Western blotting was also executed to measure the relative expressions of the proteins Bcl-2 and Bax. RNA immunoprecipitation and dual luciferase reporter experiments were carried out to validate the target relationships among MEF2C, KLF3-AS1, and miR-338-3p. Mouse xenograft models were created to assess the influences of KLF3-AS1 on the growth of tumors in vivo. Results Elevated levels of KLF3-AS1 and MEF2C and reduced amounts of miR-338-3p were identified in OS. KLF3-AS1 targeted miR-338-3p, and miR-338-3p further targeted MEF2C. Silencing KLF3-AS1 induced apoptosis and attenuated proliferation in vitro and repressed the tumor growth in vivo. Inhibiting miR-338-3p inverted the cancer-suppressing effects of KLF3-AS1 silencing. Meanwhile, loss of MEF2C partially eliminated the effects brought about by miR-338-3p downregulation, namely the stimulation of cell growth and suppression of apoptosis. Conclusions Silencing of KLF3-AS1 could repress the growth of cells and induce apoptosis by regulating miR-338-3p/MEF2C in OS. This suggests that the regulatory axis KLF3-AS1/miR-338-3p/MEF2C is a prospective target for OS treatment.
研究领域:
肿瘤学
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分子生物学
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遗传学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2024
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IF 11.2
Wenbo Yang,Qing Han,Hao Chen,Yongyue Li,Xingchen Guo,Aobo Zhang,Yang Liu,Yifu Sun,Jincheng Wang
The Voronoi-tessellation method is a promising technique for porous implant design as it mimics the irregular structure of bone trabeculae very well. However, the optimal pore size distribution of Voronoi-based trabecular-like scaffolds (VBTSs) remains unknown. In this study, three VBTSs with different pore size distributions were fabricated by Electron-beam melting (EBM), with a regular cubic scaffold as a control. Compression experiments showed that the elastic modulus of all the fabricated scaffolds was within the range of human bone. The biocompatibility of the porous scaffolds was evaluated by Cell Counting Kit-8, live/dead staining, phalloidin staining, and scanning electron microscope. The effects of scaffolds on osteogenic differentiation were evaluated by alkaline phosphatase (ALP) assay, Alizarin Red S (ARS) assay, and Real-time quantitative polymerase chain reaction (RT-qPCR). In vivo experiments were performed to evaluate the performance of bone regeneration in the scaffolds. The results showed that all scaffolds were non-toxic with good biosafety, and VBTSs were more conducive to promoting cell proliferation, osteogenic differentiation, and bone regeneration within the scaffolds. Among the 596–1044 μm range, the VBTS with an average pore size of 596 μm performed best. This study showed that bone regeneration could be regulated by controlling the porous structure and provided a reference for applying VBTSs in bone implants.
研究领域:
生物医学工程
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组织工程
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材料科学
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产品关联
DOI
高影响因子
Advanced Science
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2025
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IF 14.1
Lei Du,Sijie Liu,Yinfei Lu,Dongxue Ren,Xiujuan Yu,Yue Hu,Tingting Yang,Qun Yang,Jingxian Ming,Jiawei Zhang,Xiaoxing Yin,Qian Lu
Abnormal proliferation of mesangial cells is a hallmark of diabetic nephropathy (DN). However, the cellular signaling mechanisms that regulate this proliferation remain poorly understood. In this study, it is demonstrated that GA-binding protein (GABP), a member of the ETS family of transcription factors composed of GABPα and GABPβ, plays a significant role in the development of renal fibrosis by modulating mesangial cell proliferation. Notably, the deficiency of GABP in mesangial cells inhibits hyperglycemia-induced proliferation and mitigates renal fibrosis in a murine model of type 2 diabetes mellitus (T2DM). RNA sequencing analysis identifies GLI Family Zinc Finger 1 (GLI1) as the principal downstream effector of GABP in diabetic mice, serving as a crucial regulator of the G1/S transition within the cell cycle. Subsequent investigations have demonstrated that GABP interacts with the GLI1 promoter, facilitating mesangial cell proliferation via GLI1-dependent pathways. This is evidenced by the fact that GLI1 knockdown abrogates the proliferation of mesangial cells with GABP overexpression. Consequently, GABP emerges as a pivotal regulator of renal fibrosis and represents a promising therapeutic target for the treatment of diabetic nephropathy.
研究领域:
分子生物学
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医学
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肾脏病学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
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2022
·
IF 13.4
Xin Qi,Hua Yin,Minghan Zhu,Pengling Shao,Zhi Dang
Biochar-assisted microbial degradation technology is considered as an important strategy to eliminate organic pollutants, but the mechanism of biochar in affecting biodegradation has not been systematically studied. To address this knowledge gap, the effect of various biochars on biodegradation of different initial concentrations of BDE-47 by Pseudomonas plecoglossicida was investigated. The results showed that biochar exhibited significant promotion to the biodegradation of BDE-47, especially at concentrations of BDE-47 above 100 μg/L. The promotion effect was negatively influenced by the aromaticity and micropore volume of biochar. Biochar alleviated the cytotoxicity of BDE-47 to P. plecoglossicida and promoted cell proliferation based on toxicity assays. Additionally, biochar acted as shelter and stimulated the secretion of extracellular polymeric substances, which might support P. plecoglossicida to struggle with extreme conditions. Metabolomic analysis indicated that biochar resulted in upregulation expression of 38 metabolites in P. plecoglossicida . These upregulated metabolites were mainly related to glyoxylate and dicarboxylate metabolism, citrate cycle, and serial amino acid metabolism, suggesting that biochar could improve the BDE-47 biodegradation via enhancing oxidative metabolism and energy supply of the bacterial cells. This work elucidates how biochar can affect BDE-47 biodegradation and provides insights for the application prospect of biochar-assisted microbial degradation technology in the environment.
研究领域:
环境科学
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微生物学
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毒理学
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DOI
PubMed
高影响因子
Cancer Biomarkers
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2018
·Vol. 23
·期 4
·603-613
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IF 2.392
Liu Zhi-Biao,Tang Chen,Jin Xin,Liu Shou-Hou,Pi Wen
BACKGROUND: Small nucleolar RNA host gene 12 (SNHG12) has been shown to be a long noncoding RNA (lncRNA) that facilitates the progression of a number of malignancies. However, the expression pattern and biological function of SNHG12 in nasopharyngeal carcinoma (NPC) have not been investigated. OBJECTIVE: The aim of our study is to investigate the expression, clinical significance and function of SNHG12 in NPC. METHODS: RT-PCR was used to detect the expression of SNHG12 in NPC cell lines and primary tumor tissues. The correlation of SNHG12 with clinicopathological features and patient prognosis was analyzed. The biologic functions of SNHG12 in NPC were explored by MTT assay, colony formation assay, wound healing assays, transwell assay and flow cytometric analysis in vitro. The expression of EMT markers and Notch signal pathway markers were determined by western blotting. RESULTS: The expression levels of SNHG12 were up-regulated in both NPC tissues and cell lines. High SNHG12 expression was significantly associated with clinical stage, grade and poor prognosis. Multivariate analysis demonstrated that high lncRNA SNHG12 expression was an independent poor prognostic factor for NPC patients. Functionally, knockdown of SNHG12 suppressed NPC cells proliferation, migration and invasion. Mechanistic investigations showed that knockdown of SNHG12 suppressed the activation of EMT and Notch-1 signal pathway. CONCLUSIONS: Our data suggest that SNHG12 promotes the progression of NPC and is a potential therapeutic target for NPC intervention.
研究领域:
肿瘤学
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分子生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2018
·
IF 2.559
Jianhao Xu,Cunjin Su,Fenglun Zhao,Jialong Tao,Duanmin Hu,Aiming Shi,Jie Pan,Yusong Zhang
Paclitaxel (PTX) is a first-line chemotherapy drug for advanced non-small cell lung cancer (NSCLC). The long-chain non-coding RNA maternally expressed gene 3 (MEG3) is a recognized tumor suppressor . This study aimed to explore the effects of PTX on the expression of MEG3 and its anti-tumor mechanism in lung cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed to determine cell proliferation . Quantitative polymerase chain reaction was used to determine the levels of MEG3 expressions. Western blot and immunofluorescence were used to detect protein levels. Small interfering RNA or pCDNA-MEG3 transfection was used to downregulate or upregulate MEG3 expression. Dichlorof luorescein diacetate was used to detect intracellular reactive oxygen species. Flow cytometry was used to analyze apoptosis. PTX significantly inhibited the proliferation of NSCLC cells and increased the expressions of MEG3 and P53. The downregulation of MEG3 attenuated PTX-induced cytotoxicity, whereas upregulation of MEG3 induced cell death and increased P53 expression. The inhibition of P53 caused no effect on the upstream MEG3 expression. Our results suggest that the MEG3-P53 pathway is involved in the apoptosis of A549 cells induced by PTX.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
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2022
·
IF 6.212
Ihsan Safwan Kamarazaman,Nor Azah Mohamad Ali,Fauziah Abdullah,Nazrin Che Saad,Aida Azlina Ali,Salfarina Ramli,Pornchai Rojsitthisak,Hasseri Halim
Occurrence of the wound or chronic wound, which results to disability, amputation and diminish quality of life, are leading to increased healthcare expenditure around the globe. Despite effective conventional wound healing medicines, the exploration of alternative medicines are continuing process as researchers seek for the approach to reduce the cost of wound healing management. In present study, wound healing properties of ethanolic extract of Baeckea frutescens leaves were determined by evaluating their cytotoxicity, proliferation and migration rate on two types of cells, keratinocytes (HaCaT) and fibroblasts (BJ). Furthermore, the antioxidant properties of this plant were determined by DPPH scavenging, ferric reducing antioxidant power (FRAP) and total phenolics content (TPC) assays. The phytochemistry of the extract was evaluated by phytochemicals screening and liquid chromatography mass spectrometry (LCMS) analysis. Results of this study indicated Baeckea frutescens extract increased the rate of proliferation and migration on both HaCat and BJ cells within their nontoxic doses. The extract also possessed a very good antioxidant property as demonstrated with high DPPH radical scavenging, FRAP and TPC values, comparable to that of Green tea extract, a widely known antioxidant. The phytochemistry analyses and LCMS exhibited the presence saponins, flavonoids, tannins and steroids in Baeckea frutescens extract possibly responsible to their antioxidant and wound healing properties.
研究领域:
药理学
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植物化学
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DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 6
·3698-3711
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IF 3.9
Jing Zhan,Li Li,Lili Yao,Zheng Cao,Weiwei Lou,Jianying Zhang,Jinsong Liu,Litao Yao
Modifying the drug-release capacity of titanium implants is essential for maintaining their long-term functioning. Titanium dioxide nanotube (TNT) arrays, owing to their drug release capacity, are commonly used in the biomaterial sphere. Their unique half open structure and arrangement in rows increase the drug release capacity. However, their rapid drug release ability not only reduces drug efficiency but also produces excessive local and systemic deposition of antibiotics. In this study, we designed a tantalum-coated TNT system for drug-release optimization. A decreased nanotube size caused by the tantalum nanocoating was observed through SEM and analyzed (TNT: 110 nm, TNT-Ta1: 80 nm, TNT-Ta3: 40 nm, TNT-Ta5: 20 nm, TNT-Ta7: <5 nm). XPS analysis revealed the distribution of the chemical components, especially that of the tantalum element. In vitro experiments showed that the tantalum nanocoating enhanced cell proliferation; in particular, TNT-Ta5 possessed the best cell viability (about 1.18 of TNT groups at 7d). It also showed that the tantalum nanocoating had a positive effect on osteogenesis (especially TNT-Ta5 and TNT-Ta7). Additionally, hydrophilic/hydrophobic drug (vancomycin/raloxifene) release results indicated that the TNT-Ta5 group possessed the most desirable sustained release capacity. Moreover, in this drug release system, the hydrophobic drug showed more sustained release capacity than the hydrophilic drug (vancomycin: sustained release for more than 48 h, raloxifene: sustained release for more than 168 h). More importantly, TNT-Ta5 is proved to be an appropriate drug release system, which possesses cytocompatibility, osteogenic capacity, and sustained drug release capacity.
研究领域:
生物材料学
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医学工程
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组织工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）