DYES AND PIGMENTS
·
2016
·
IF 4.055
Yue Li,Fengli Zhang,Xiao-Feng Wang,Gang Chen,Xueqi Fu,Wenjing Tian,Osamu Kitao,Hitoshi Tamiaki,Shin-ichi Sasaki
Herein three synthesized chlorophyll derivatives (ZnChl-1, H 2 Chl-2, ZnChl-2) featuring hydrophilic hydroxy/carboxy groups were employed as precursors for fluorescent organic nanoparticles (NPs) with biocompatible Pluronic F127 as the encapsulation matrix for the first time. Such red NPs exhibited broad absorption bands and high hydrophilicity . Three chlorophyll derivatives with different substituent groups show varying degrees of changes after loaded with F127. As a result, ZnChl-2 exhibited the best retainable photoluminescence yield after encapsulation among these chlorophyll derivatives. It reveals that ZnChl-2@F127 is the most suitable NPs for cell imaging by comparing not only different three chlorophyll derivatives, but also free ZnChl-2 and ZnChl-2@F127. Application of ZnChl-2@F127 NPs for cancer cell imaging was successfully demonstrated and such chlorophyll-based NPs showed bright fluorescence signals and low toxicity. These studies indicate that the F127 encapsulated low-costed chlorophyll derivatives NPs are efficient fluorescent dyes for biological imaging. It is further directed towards photodynamic therapy based on chlorophyll derivatives.
研究领域:
生物医学工程
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材料科学
·
光学成像
✓ 已验证
DOI
高影响因子
Chemosensors
·
2021
·Vol. 9
·期 3
·48
·
IF 3.398
Wei Wang,Hongren Zhao,Bing Zhao,Huimin Liu,Qinglei Liu,Yan Gao
In this paper, a novelNL-Fe3+ensemble was designed as a fluorescent chemosensor for highly selective detection of pyrophosphate (PPi) in DMSO/H2O (2:8/v:v, pH = 7.2) solution and living cells.NLshowed a strong affinity for Fe3+and was accompanied by obvious fluorescence quenching. Upon the addition of PPi to the generatedNL-Fe3+ensemble, the fluorescence and absorption spectra were recovered completely. Spectroscopic investigation showed that the interference provoked by common anions such as adenosine-triphosphate (ATP), adenosine diphosphate (ADP), and phosphates (Pi) can be ignored. The detection limit ofNL-Fe3+to PPi was calculated to be 1.45 × 10−8M. Intracellular imaging showed thatNL-Fe3+has good membrane permeability and could be used for the detection of PPi in living cells. A B3LYP/6-31G(d,p) basis set was used to optimizeNLandNL-Fe3+complex.Keywords:coumarin;Fe(III);pyrophosphate;cells imaging
研究领域:
生物化学
·
分析化学
·
细胞生物学
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DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 10
·e4914
·
IF 3.2
Lingjie Hou,Linlin Wang,Chenhua Deng,Pengyue Jin,Chaochao Wen,Wenjia Zhang,Wenting Liang
Ca 2+ ion as a second messenger in signaling pathway plays many vital roles in many biological phenomena. Thus, it is of significance for developing effective probes to detect Ca 2+ ion specifically. Herein, a new Schiff base fluorescent probe FPH , fluorescein monoaldehyde (2-aminomethylpyridine) hydrazone, was designed and synthesized to identify Ca 2+ in DMSO aqueous solution. The probe FPH revealed significant responses to Ca 2+ with a fluorescence enhancement at 540 nm, exhibiting an evident fluorescence change from ultraweak luminescence to bright green. Otherwise, the FPH displayed a good linear range of 0.67 × 10 −6 to 3.33 × 10 −6 mol/L with a lower detection limit at 7.02 × 10 −8 mol/L. The probe FPH were further successfully utilized to detect Ca 2+ in living cells by an increased bright green fluorescence.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 412
·期 4
·881-894
·
IF 3.286
Xiao Li,Sun Qi,Zhao Qiang,Cheng Xinjian
The detection of heavy metals such as Hg 2+ and Ag + is important and urgent. In this work, – NO 2 /– NH 2 /C=S boron dipyrromethene small molecular derivatives were synthesized at first. Then they were incorporated into polymer chains. The macromolecular fluorescent probes were obtained via Sonogashira reaction using the small molecular probes as building blocks. The as-prepared small-molecule fluorescent probe BO3 exhibits high sensing performance for Hg 2+ . By introducing it into macromolecules, the sensing ability still remains, and even more, the recognition performance is improved. The macromolecular fluorescent probes P1 , P2 , and P3 also have high recognition ability for Ag + with a binding ratio of 2:1 (metal ion to probe ratio). Through the study of the sensing mechanism and the recycling experiments, it is found that the probes responded by the photo-induced electron transfer mechanism and can be recycled and reused. At the same time, BO3 , P2 , and P3 show excellent recognition performance for Hg 2+ in living cells and zebrafish. Living cell imaging experiments indicated that these fluorescent probes had good cell membrane permeability and low cytotoxicity, and could realize bioimaging of Hg 2+ . Therefore, the application value of these fluorescent probes could be enlarged.
Graphical abstract
研究领域:
化学
·
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
·
2024
·
IF 2
Ling Li,Yinuo Yao,Xiaowan Wang,Yaoqi Yu,Tailong Ji,Han Li,Yi Li,Yuanyuan Liu
A highly selective fluorescent sensor Por-RBO for determination of Hg 2+ was designed and synthesized with Rhodamine B and Tetraphenylporphyrin. The sensor can determine the Hg 2+ of a solution within the pH 5.0–8.0, free from interference of other metal ions. When detected in buffer solution, the fluorescence was the strongest when five times the concentration of Hg 2+ ion was added. Calculated by fluorescence titration method, the detection limit of sensor Por-RBO for Hg 2+ ion was as low as 0.12 μmol/L. The geometries of Por-RBO and Por-RBO -Hg 2+ were optimized at the B3LYP/6-31G** level by density functional theory. The charge distribution, orbital interactions, and bonding characteristics were analyzed and compared in detail to discuss the recognition mechanism and structure–fluorescence property relationships. The results of cell fluorescence imaging and CCK-8 experiments indicate that the sensor Por-RBO exhibits lower cytotoxicity. Por-RBO can be used for fluorescence distribution imaging of Hg 2+ in living cells.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Dan Li,Yadan Ma,Huazhen Duan,Fei Jiang,Wei Deng,Xingang Ren
A fluorescent and surface-enhanced Raman spectroscopy (SERS) dual-mode probe is developed for imaging of intracellular Zn 2+ based on N-(2-(bis(pyridine-2-ylmethyl)amino)ethyl)-2-mercaptoacetamide (MDPA) modified gold nanoparticles (MDPA-GNPs). Benefiting from the chelation-enhanced fluorescence (CHEF) between MDPA-GNPs and Zn 2+ , the fluorescent intensities of MDPA-GNPs are substantially enhanced with the increment of Zn 2+ concentrations, which can be clearly observed by the naked eye. Under physiological conditions, the probe exhibits a stable response for Zn 2+ from 1 μM to 120 μM, with a detection limit of 0.32 μM in aqueous solutions. The resultant MDPA-GNPs can be used for ultrasensitive SERS detection of Zn 2+ because of the strong inter-particle plasmonic coupling generated in the process of Zn 2+ -triggered MDPA-GNPs self-aggregation, with a low detection limit of 0.28 pM, which is eight order of magnitude lower than the United States Environmental Protection Agency (US EPA)-defined limit (76 μM) in drinkable water. More importantly, the proposed probe can be applied for efficient detection of intracellular Zn 2+ with excellent biocompatibility and cellular imaging capability. Therefore, a highly sensitive and selective nanosensor has been demonstrated for both reliable quantitative detection of Zn 2+ in aqueous solution and real-time imaging of intracellular Zn 2+ , suggesting its significant potential utility in bioanalysis and biomedical detection in the future.
研究领域:
生物分析
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生物医学
·
化学传感
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 18
·7646-7652
·
IF 1.821
Sheng Chen,Chao Ma,Dong-En Wang,Xiang Han,Longlong Zhang,Jinyi Wang
Hydrogen sulfide (H2S) is the third endogenous gasotransmitter, following nitric oxide (NO) and carbon monoxide (CO). Efficient methods for selective monitoring H2S in biological systems are significant. In this study, we designed and synthesized a new coumarin-based probe DNPOCA for H2S with colorimetric and fluorescent dual signaling. In this probe, 7-hydroxycoumarin-4-acetic acid methyl ester was employed as a fluorophore and a dinitrophenyl (DNP) ether moiety was used as the recognition unit. The H2S probe exhibited excellent selectivity and high sensitivity with a detection limit as low as 49.7 nM. The cytotoxicity assay showed that DNPOCA possessed a low effect on cell viability. Furthermore, DNPOCA could be successfully applied for H2S imaging in living cells.
研究领域:
生物化学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2022
·Vol. 70
·期 1
·68-75
·
IF 1.753
Yuanyuan Cui,Yaoyang Meng,Yun Hu,Yuqi Chen,Yixian Yang,Min Fang,Cun Li,Weiju Zhu
A novel fluorescent probe, LCH , based on dicyanisophorone and carbazole, was prepared for the visual detection of Cu 2+ . The probe LCH could recognize Cu 2+ by fluorescence quenching in EtOH/H 2 O (1/4, v/v) solution, which could be easily identified under the 365 nm UV lamp, and the detection limit was as low as 0.785 μM. The recognition mechanism of probe LCH with Cu 2+ was determined by combining 1 H NMR titration, MS, and theoretical calculations. Practical application experiments showed that probe LCH could be used to detect Cu 2+ in the test strip experiments. Cell imaging experiments showed that the probe LCH owned good cell permeability and could be applied to the imaging of Cu 2+ in HepG2 cells. In addition, fluorescence colocalization experiments showed that LCH could target lipid droplets. These results indicate that the probe LCH will have a good application prospect in environmental detection and clinical medicine.
研究领域:
分析化学
·
生物医学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yuntong Huang,Weizhong Chen,Mingyou Dong,Nana Li,Lianghui Chen,Li Ling,Qijiang Xu,Min Lin,Zhiyong Xing
A facile fluorescence probe BQBH was synthesized and investigated on its spectrum property. The result showed that the BQBH had high sensitivity and selectivity for Cd 2+ with lowest detection determined as 0.14 μM by fluorescence response. The 1: 1 binding ratio between BQBH and Cd 2+ was determined by Job’s plot, and the binding details were further confirmed by 1 H NMR titration, FT-IR spectrum and HRMS analysis. The applications including on test paper, smart phone and cell image were all also investigated.
研究领域:
分析化学
·
生物医学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 14
·8250-8254
·
IF 3.361
Huiying Chen,Jianzhi Zhao,Junzhi Lin,Baoli Dong,Hui Li,Bing Geng,Mei Yan
Lipid droplets (LDs) are storage organelles at the centre of lipid and energy homeostasis, which act as vital hubs of cellular metabolism and the key to maintaining lipid and energy homeostasis. We synthesized a new two-photon fluorescent probe (CIV) that could detect the viscosity of lipid droplets. The probe is constructed via the typical ICT system of D–π–A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluorescence intensity of CIV increased by 13-fold, showing sensitivity and specificity towards viscosity. In addition, CIV showed low toxicity and excellent biocompatibility in cytotoxicity tests, and was successfully used for living cell LD imaging. Taken together, the results widen the way for the development of novel fluorescent probe-based the visualization LDs and detection in solutions, physiology and pathology.
研究领域:
生物化学
·
细胞生物学
·
医学影像学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 9
·3883-3887
·
IF 1.821
Hao-Ming Lv,Yi Chen,Jian Lei,Chak-Tong Au,Shuang-Feng Yin
Nitroxyl is a highly reactive compound that irreversibly dimerizes and subsequently dehydrates to yield nitrous oxide and water, making its direct detection in cells or in vivo difficult. In this paper, a novel fluorescent probe 1 based on the excited state intramolecular proton transfer (ESIPT) mechanism for the detection of nitroxyl was developed for the first time. It consists of 2-(2′-hydroxyphenyl)-benzothiazole as the fluorophore, linked via an ester moiety to a diphenylphosphinobenzoyl group. The probe exhibited high sensitivity and selectivity toward nitroxyl in the presence of biologically relevant species. Moreover, the potential for biological applications of the probe was confirmed by employing it for fluorescence imaging of nitroxyl in living cells.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Yue Zhao,Mingya Wang,Junli Shi,Mingfeng Han,Yuqi Wu,Shengling Li,Linxiu Zhao,Duanlin Cao
In this paper, a fluorescent probe methyl( E )-2-(3-(benzo[ d ]thiazol-2-yl)-2‑hydroxy -5-methylbenzylidene) hydrazine-1-carboxylate ( DYH ) based on benzothiazole was designed and synthesized. The characterization involved NMR, HRMS, and crystal structure analysis. In case of the existence of other competing cations, the probe DYH showed high-affinity for Cu 2+ , and the resultant DYH Cu 2+ showed exceptional capacity to recognize S 2− in CH 3 CN/HEPES (7/3, v/v, HEPES 0.1 M, pH = 7.3). The detection limits of DYH for Cu 2+ and DYH Cu 2+ for S 2− were 0.56 μM and 0.67 μM, respectively. It was determined that the binding stoichiometry between the DYH and Cu 2+ , as well as DYH Cu 2+ and S 2− , was 1:1 according to the job's curve. The pH of the probe and complex was stable between 4 and 10. Colorimetric experiments of the probe DYH were conducted using test paper as a carrier. Additionally, DYH was used for fluorescence imaging of Cu 2+ and S 2− in Hela cells with poor cellular toxicity and good cell permeability.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
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