CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yonggan Xue,Jimei Chi,Bobin Ning,Lijun Cheng,Wenchen Wang,Qingyu Meng,Liang Huang,Mu Ke,Sen Li,Meng Su,Baoqing Jia
Due to non-specific signs in the early stage of colorectal cancers, most patients result in a low early diagnosis rate. Survivin protein is upregulated in many malignant tumors, making it an ideal diagnostic and therapeutic target for colorectal cancers. However, the survivin protein is located in the cell nucleus, which needs the probe to penetrate the complex nuclear membrane structure for targeting and recognizing. Here, we have developed a sub-100 nm perovskite-based fluorescent probe modified with the survivin antibody, which can freely target survivin protein through the nuclear membrane. Polyvinyl pyrrolidone (PVP) coated perovskite crystals are obtained by the supersaturated crystallization, which keep the probe with great fluorescence intensity and resistance of water and oxygen. We have established colorectal cancer cell lines and colorectal cancer tissues to test the feasibility and efficacy of the probe. After investigating the fluorescence intensity values of 20 probing samples, the average fluorescence intensity of colorectal cancer sections is 8.7 times higher than that of paracancerous tissues. Our work demonstrates that the survivin can be an ideal fluorescence target for rapid diagnosis of colorectal cancer, which also provides a research foundation for fluorescence-guided surgery and nano-therapy for targeting the survivin protein.
研究领域:
肿瘤学
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分子生物学
·
医学影像学
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DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Long Cheng,Zhixin Xie,Ming Cao,Shuaifan Qin,Yongshi Chen,Bo Tian,Zhibiao Feng
As a new type of nanomaterial, carbon dots (CDs) have demonstrated potential in the field of biological imaging. Currently, research utilizing them for labeling microorganisms is confined to simple imaging, with limited studies exploring their application in detecting cell viability and organelle targeting. Yeast, serving as both a fermenting agent and a model cell, constitutes an ideal research subject This study synthesized ascorbic acid-based CDs (A-CDs) using citric acid, ascorbic acid, and ethylenediamine as raw materials via hydrothermal method. A-CDs were uniformly dispersed, with an average particle size of 3.28 nm. The surface functional groups, such as NH 2 and -OH, endowed them with hydrophilicity. Additionally, A-CDs exhibited concentration-dependence, good photostability, and biocompatibility. By labeling yeast with A-CDs, it was observed that cells in dead, alive, and intermediate states exhibited different imaging effects, which were highly correlated with the concentration of A-CDs entering the cell due to variations in cell membrane permeability. Through co-localization experiments with A-CDs and DAPI, it was confirmed that A-CDs could target and label cell nucleus. Preliminary endocytosis experiments indicated that A-CDs primarily entered cells via clathrin-mediated and lipid raft-mediated endocytosis. The ability of A-CDs to discriminate between cell viability and label nuclei offers new perspectives on the application of CDs in the field of biological imaging.
研究领域:
生物成像
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纳米材料科学
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微生物学
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产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 14
·6104-6113
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IF 7.394
Lei Yang,Weihua Jiang,Lipeng Qiu,Xuewei Jiang,Daiying Zuo,Dongkai Wang,Li Yang
Strong blue fluorescent polyethylene glycol (PEG) anchored carbon nitride dots (CDs@PEG) with a high quantum yield (QY) of 75.8% have been synthesized by a one step hydrothermal treatment. CDs with a diameter of ca. 6 nm are well dispersed in water and present a graphite-like structure. Photoluminescence (PL) studies reveal that CDs display excitation-dependent behavior and are stable under various test conditions. Based on the as-prepared CDs, we designed novel cell nucleus targeting imaging carbon dots functionalized with a nuclear localization signal (NLS) peptide. The favourable biocompatibilities of CDs and NLS modified CDs (NLS-CDs) are confirmed by in vitro cytotoxicity assays. Importantly, intracellular localization experiments in MCF7 and A549 cells demonstrate that NLS-CDs could be internalized in the nucleus and show blue light, which indicates that CDs may serve as cell nucleus imaging probes.
研究领域:
生物化学
·
材料科学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2016
·Vol. 8
·期 12
·6801-6809
·
IF 7.76
Lei Yang,Zheran Wang,Ju Wang,Weihua Jiang,Xuewei Jiang,Zhaoshi Bai,Yunpeng He,Jianqi Jiang,Dongkai Wang,Li Yang
Carbon dots (CDs) have shown great potential in imaging and drug/gene delivery applications. In this work, CDs functionalized with a nuclear localization signal peptide (NLS-CDs) were employed to transport doxorubicin (DOX) into cancer cells for enhanced antitumor activity. DOX was coupled to NLS-CDs (DOX-CDs) through an acid-labile hydrazone bond, which was cleavable in the weakly acidic intracellular compartments. The cytotoxicity of DOX-CD complexes was evaluated by the MTT assay and the cellular uptake was monitored using flow cytometry and confocal laser scanning microscopy. Cell imaging confirmed that DOX-CDs were mainly located in the nucleus. Furthermore, the complexes could efficiently induce apoptosis in human lung adenocarcinoma A549 cells. The in vivo therapeutic efficacy of DOX-CDs was investigated in an A549 xenograft nude mice model and the complexes exhibited an enhanced ability to inhibit tumor growth compared with free DOX. Thus, the DOX-CD conjugates may be exploited as promising drug delivery vehicles in cancer therapy.
研究领域:
药学
·
生物医学工程
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN TOXICOLOGY
·
2020
·
IF 3.184
Yulian Chen,Tao Zhang,Lili Wu,Yilin Huang,Zhihao Mao,Zhikun Zhan,Weizhong Chen,Fahong Dai,Wenyu Cao,Yong Cao,Shuwen Liu,Zheng Cai,Lan Tang
Emodin is the main toxic component in Chinese medicinal herbs such as rhubarb. Our previous studies demonstrated that genetic polymorphisms of UDP-glucuronosyltransferase 2B7 (UGT2B7) had an effect on the glucuronidation and detoxification of emodin. This study aimed to reveal the transcriptional regulation mechanism of UGT2B7 on emodin glucuronidation and its effect on toxicity. Emodin glucuronic activity and genome and transcriptome data were obtained from 36 clinical human kidney tissues. The genome-wide association studies (GWAS) identified that four single nucleotide polymorphisms (SNPs) (rs6093966, rs2868094, rs2071197, and rs6073433), which were located on the hepatocyte nuclear factor 4α (HNF4A) gene, were significantly associated with the emodin glucuronidation (p < 0.05). Notably, rs2071197 was significantly associated with the gene expression of HNF4A and UGT2B7 and the glucuronidation of emodin. The gene expression of HNF4A showed a high correlation with UGT2B7 (R2 = 0.721, p = 5.83 × 10–11). The luciferase activity was increased 7.68-fold in 293T cells and 2.03-fold in HepG2 cells, confirming a significant transcriptional activation of UGT2B7 promoter by HNF4A. The knockdown of HNF4A in HepG2 cells (36.6%) led to a significant decrease of UGT2B7 (19.8%) and higher cytotoxicity (p < 0.05). The overexpression of HNF4A in HepG2 cells (31.2%) led to a significant increase of UGT2B7 (24.4%) and improved cell viability (p < 0.05). Besides, HNF4A and UGT2B7 were both decreased in HepG2 cells and rats after treatment with emodin. In conclusion, emodin used long term or in high doses could inhibit the expression of HNF4A, thereby reducing the expression of UGT2B7 and causing hepatotoxicity.
研究领域:
药理学
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遗传学
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毒理学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2017
·Vol. 7
·期 45
·28016-28023
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IF 3.108
Huabing Liu,Yanli Bai,Yanrong Zhou,Chenchen Feng,Lingzhi Liu,Liurong Fang,Jiangong Liang,Shaobo Xiao
Carbon dots (CDs) have demonstrated attractive potential in cell imaging and tracking. However, one of the most challenge problems is the selective entrance of CDs into the cytoplasm or cell nucleus. Here, a facile strategy was developed to prepare two types of CDs capable of selective entrance into cytoplasm and the whole cell without further surface modification. The surface charge of CDs changes from negative to neutral after the addition of urea in the hydrothermal process, and the as-prepared CDs exhibit different fluorescent colors. The blue-fluorescent CDs (b-CDs) can only enter the cytoplasm, while the cyan-fluorescent CDs (c-CDs) can distribute over the whole cell, including the nucleus. Additionally, the antiviral activity of the two CDs against pseudorabies virus (PRV) was studied and b-CDs were shown to have better antiviral activity. This study has provided a facile and novel strategy for the fabrication of CDs used in selective cell imaging, which may facilitate the application of CDs in nanomedicine.
研究领域:
生物医学
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纳米医学
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病毒学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yuanyuan Qin,Mengling Shen,Xinpei Liu,Jingyu Gu,Minqian Zhu,Xuan Yi
As a kind of high linear energy transfer (LET) radiation, internal conversion electrons are emitted from some radionuclides, such as 125I, triggering severe DNA damage to tumor cells when transported into the nucleus. Herein, we develop a curcumin-loaded nanomicelle composed of a photosensitizer chlorin e6 (Ce6) and amphiphilic poly(ethylene glycol) (poly(maleic anhydride-alt-1-octadecene)-poly(ethylene glycol) (C18-PMH-PEG)) to deliver 125I into the nucleus under 660 nm laser irradiation, leading to the optimized imaging-guided internal conversion electron therapy of cancer. Ce6-containing nanomicelles (Ce6-C18-PEG) self-assemble with nucleus-targeted curcumin (Cur), obtaining Ce6-C18-PEG/Cur nanoparticles. After labeling Cur with 125I, Ce6-C18-PEG/Cur enables single-photon emission computed tomography and fluorescence imaging of the tumor, serving as a guide for follow-up laser irradiation. Notably, the 660 nm laser-triggered photodynamic reaction of Ce6 optimizes the delivery of Ce6-C18-PEG/125I-Cur at various stages, including tumor accumulation, cellular uptake, and lysosome escape, causing plenty of 125I-Cur to enter the nucleus. By this strategy, Ce6-C18-PEG/125I-Cur showed optimal antitumor efficacy and high biosafety in mice treated with local 660 nm laser irradiation using efficient energy deposition of internally converted electrons over short distances. Therefore, our work provides a novel strategy to optimize 125I delivery for tumor treatment.
研究领域:
放射生物学
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 78
·63330-63337
·
IF 3.84
Yang Ding,Qiong Wu,Kangdi Zheng,Linkun An,Xiaoying Hu,Wenjie Mei
Two novel ruthenium(II) complexes coordinated with 6-chloro-5-hydroxylpyrido[3,2-a]phenazine (CQM), [Ru(L)2(CQM)]ClO4 [L = 1,10-phenanthroline, (1) and 2,2′-bipyridine, (2)], were investigated as potential fluorescent probes to track dynamic changes in the nuclei of living cells. Confocal laser technology was used to observe their co-location inside the cells. The results showed that both complexes were taken up by HepG2 cells, especially 1, which was localized in the cell nuclei, whereas 2 was distributed in the cell nuclei and mitochondria. Further studies by real-time fluorescence observations revealed that 1 rapidly entered the living cells, namely, HepG2, HeLa and MCF-7 cells, imaged the dynamic changes of the nuclei of living tumor cells, and exhibited low toxicity toward cells. The results demonstrated that 1 may be developed into a novel fluorescent probe for living cell nuclei. This study facilitates the development of fluorescent chemosensors with metal complexes.
研究领域:
生物化学
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细胞生物学
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肿瘤学
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产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2015
·Vol. 44
·期 42
·18671-18676
·
IF 4.197
Quankun Gao,Bo Song,Zhiqiang Ye,Liu Yang,Ruoyang Liu,Jingli Yuan
In this work, we designed and synthesized a heterobimetallic ruthenium(II)–nickel(II) complex, [Ru(bpy)2(phen-DPA)Ni](PF6)4 (Ru–Ni), as a highly selective phosphorescence probe for histidine. The probe exhibited weak emission at 603 nm because the phosphorescence of the Ru(II) complex can be strongly quenched by the paramagnetic Ni2+ ion. In the presence of histidine, reaction of Ru–Ni with histidine resulted in the release of nickel(II) and an enhancement in the phosphorescence intensity at 603 nm. Ru–Ni showed high selectivity for histidine even in the presence of other amino acids and cellular abundant species. Cell imaging experimental results demonstrated that Ru–Ni is membrane permeable, and can be applied for visualizing histidine in live cells. More interestingly, Ru–Ni also can act as a novel reaction-based nuclear staining agent for visualizing exclusively the nuclei of living cells with a significant phosphorescence enhancement. In addition, the potential of the probe for biological applications was confirmed by employing it for phosphorescence imaging of histidine in larval zebrafish and Daphnia magna. These results demonstrated that Ru–Ni would be a useful tool for physiological and pathological studies involving histidine.
研究领域:
生物化学
·
分析化学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Haifang Liu,Jie Yang,Zhaohui Li,Lehui Xiao,Aaron Albert Aryee,Yuanqiang Sun,Ran Yang,Hongmin Meng,Lingbo Qu,Yuehe Lin,Xiaobing Zhang
Carbon dots (CDs) are emerging as powerful tools for biosensing and bioimaging because of their intrinsic properties such as abundant precursors, facile synthesis, high biocompatibility, low cost, and particularly robust tunability and stability. In this work, a new type of CDs was prepared from m-phenylenediamine and folic acid by hydrothermal method. Interestingly, the as-prepared CDs show blue emission in non-hydrogen-bonding solution, whereas robust green emission in hydrogen-bonding solution. Based on this phenomenon, a novel fluorescence sensing mechanism named as hydrogen-bonding-induced emission (HBIE) was proposed. The HBIE-CDs have large Stokes shift (141 nm) in water, good biocompatibility, and ultrasmall size, which facilitates their translocation into living cells. Very importantly, the as-prepared HBIE-CDs show strong affinity toward nucleic acid without interference from other biological species. After binding with DNA/RNA through hydrogen bond, as high as 6-fold green fluorescence enhancement of HBIE-CDs was observed. Since the nucleus is rich in DNA/RNA, these HBIE-CDs were successfully used for rapid and, especially, wash-free subcellular in situ imaging of the nucleus in living cells in a fluorescence turn on mode, which has a great practicability to be used for nucleus imaging in bioanalytical studies and clinical applications.
研究领域:
材料科学
·
生物医学工程
·
分析化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 14
·3396
·
IF 4.2
Zhengyang Wang,Shuting Wang,Yuexing Zhang,Mingliang Ma
A series of fluorescent molecules with 1,1-dimethylnaphthalene-2(1H)-one as the core were synthesized to overcome aggregation quenching and emit bright green fluorescence. The low molecular weight of these molecules led to them to smoothly pass through the cell membrane and penetrate deep into the nucleus to emit the corresponding fluorescence. Among them, NC-4-Br and NC-5-3O have good optical and in vitro properties and showed potential for use as fluorescent probes.
研究领域:
生物化学
·
分子生物学
·
医学成像
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
Riliga Wu,Hongyu Dai,Weijiang Guan,Chao Lu
Clusteroluminescence (CL) clusters in the absence of aromatic or π-conjugated structures have emerged as a new family of luminescent materials due to their abundant sources and inherent biocompatibility. However, there is an inborn challenge in controlling the size of CL clusters from random aggregation of micrometers to ordered assembly of sub-10 nanometers. Such an inherent drawback significantly restricts their progress from theoretical research to practical applications. To address this obstacle, a novel approach using space-confined templates is proposed to synthesize CL clusters with tunable sizes. Space-confined templates with sizes of ≈2–6 nm are constructed to effectively confine non-aromatic molecules, yielding size-controllable and luminescence-tunable CL clusters. The versatility of this synthetic strategy is further proved by using non-conjugated molecules, such as L-valine and L-isoleucine. Finally, the potential applications of the synthesized CL clusters have been implemented in cell nucleus imaging owing to their sub-10 nm size and efficient luminescence. The success of this work not only offers a versatile space-confined approach to synthesize size-tunable CL clusters within sub-10 nm, but also opens new avenues for the deployment of CL clusters in advanced applications.
研究领域:
材料科学
·
生物医学工程
·
化学合成
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）