SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Chenxin Lyu,Daofan Tang,Chengqian Zhang,Jun Xie,Qingyan Zhang,Jing Nie,Yong He,Jianzhong Fu,Jingdai Wang,Peng Zhao
Non-contact manipulation and measurement of cells are the major concerns in the biomedical field . However, the existing methods are limited by the physical properties of cells, and may cause damages to the cells. This paper proposes an original non-contact manipulation and measurement method by magnetic levitation . Based on the configuration of two ring magnets with like poles facing each other, the cells can aggregate and levitate in the paramagnetic medium. The mathematical model was established to analyze the distributions of magnetic fields and movements of cells for the first time. Theoretical and experimental results show that the magnetic levitation method can be adaptive to the cells by tuning the size of the configuration. The three-dimensional cell clusters were formed and the average densities of 3T3, MDA, B16 and 4T1 cells were measured as 1.074 ± 0.005 g/cm 3 , 1.084 ± 0.003 g/cm 3 , 1.079 ± 0.002 g/cm 3 and 1.124 ± 0.003 g/cm 3 respectively. Moreover, the dead cells and living cells were distinguished based on the levitating heights. The results indicate that the proposed magnetic levitation method is promising for the three-dimensional culture and rapid measurement of cells.
研究领域:
生物医学工程
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Jia Yang,Fang Zhou,Rubo Xing,Yuan Lin,Yanchun Han,Chunbo Teng,Qian Wang
The generation of transplantable β-cells from pancreatic progenitor cells (PPCs) could serve as an ideal cell-based therapy for diabetes. Because the transplant efficiency depends on the size of islet-like clusters, it becomes one of the key research topics to produce PPCs with controlled cluster sizes in a scalable manner. In this study, we used inkjet printing to pattern biogenic nanoparticles, i.e., mutant tobacco mosaic virus (TMV), with different spot sizes to support the formation of multicellular clusters by PPCs. We successfully achieved TMV particle patterns with variable features and sizes by adjusting the surface wettability and printing speed. The spot sizes of cell-adhesive TMV mutant arrays were in the range of 50–150 μm diameter. Mouse PPCs were seeded on the TMV-RGD (arginine–glycine–aspartate)-patterned polystyrene (PS) substrate, which consists of areas that either favor (TMV-RGD) or prohibit (bare PS) cell adhesion. The PPCs stably attached, proliferated on top of the TMV-RGD support, thus resulting in the formation of uniform and confluent PPC clusters. Furthermore, the aggregated PPCs also maintained their multipotency and were positive for E-cadherin, indicating that the formation of cell–cell junctions is critical for enhanced cell–cell contact.
研究领域:
生物医学工程
·
细胞生物学
·
糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Weiya Zeng,Ying Wang,Qing Zhang,Chengyi Hu,Jing Li,Jinwei Feng,Chenglu Hu,Yong Su,Jie Lou,Ling Long,Xing Zhou
Cancer metastasis is the main cause of cancer-related deaths and involves the interaction between tumor cells and neutrophils. In this study, we developed activated neutrophil membrane-coated nanoparticles (aNEM NPs) as nanodecoys to block neutrophil-mediated cancer metastasis. The aNEM NPs were fabricated by cloaking poly(lactic acid) nanoparticles with membranes derived from activated neutrophils and inherited the functional proteins of activated neutrophils. We demonstrated that aNEM NPs could interfere with the recruitment of neutrophils to the primary tumor and premetastatic niches, inhibit the adhesion of neutrophils to tumor vascular endothelium and circulating tumor cells (CTCs), and disrupt the formation of CTC–neutrophil clusters in vitro and in vivo. In 4T1-bearing mice, aNEM NPs could effectively reduce breast cancer metastasis to various organs in mice. Our results suggest that aNEM NPs are a promising nanomedicine for preventing or treating cancer metastasis by acting as neutrophil nanodecoys.
研究领域:
肿瘤学
·
纳米医学
·
生物医学工程
·
癌症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2021
·
IF 4.384
Lulu An,Jimusi Wuri,Zhitong Zheng,Wenqui Li,Tao Yan
Chemotherapy has several adverse effects to patients, some of which are life-threatening. We hypothesized that Doxorubicin induced microbiome imbalance and intestinal damage may contribute to Doxorubicin induced cardiac dysfunction. Male adult (2–3 months) C57BL/6 mice were administered 3 mg/kg, 5 mg/kg, 7.5 mg/kg,15 mg/kg, 20 mg/kg doses of Doxorubicin. Echocardiography was performed at 7 and 14 days after Doxorubicin administration. 16S rRNA amplicon sequencing was used to characterize microbiome changes. Fecal microbiota transplantation (FMT) was performed to evaluate the role of the microbiota on Doxorubicin induced cardiac dysfunction. Doxorubicin dose dependently increases mortality rate and induces cardiac dysfunction. 5 mg/kg-Doxorubicin significantly induces decreased left ventricular ejection fraction (LVEF) and fraction shortening (FS) as well as increased cardiac fibrosis , inflammation and oxidative stress respond without increasing mortality. 5 mg/kg-Doxorubicin induces significant decreased colorectum length, increased loss of goblet cells, numbers of ulcers and infiltration of lymphocyte clusters and decreased tight junction protein ZO-1, as well as increased plasma endotoxin level measured by ELISA assay. 16S rRNA microbiota analysis shows that Doxorubicin-induced microbiota dysbiosis with decreased community richness compared with normal control mice. FMT to Doxorubicin-5 mg treated mice significantly improved cardiac function by increasing LVEF and FS as well as decreased perivascular and interstitial fibrosis ; increased colorectum length, decreased the loss of goblet cells,infiltration of lymphocyte clusters,the number of ulcers and plasma endotoxin level; improved microbiota composition, function and diversity with increased abundance of Alloprevotella, Prevotellaceae_UCG−001 and Rikenellaceae_RC9_gut_group. We find that normal fecal transplantation improves cardiac function, decreases gut damage and alter microbiota composition induced by Doxorubicin. The microbiota appears to contribute to heart-gut interaction.
研究领域:
药理学
·
心脏病学
·
微生物学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）