JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2012
·
IF 2.625
Chi Ma,Di Pang,Zuochun Xiong,Wei Bai,Chengdong Xiong
Control of cellular responses is crucial for the use of electrospun membranes in biomedical applications, including tissue engineering or biomedical devices. However, it is still unclear whether adhesion and proliferation of fibroblasts is stimulated or inhibited on polyethylene glycol (PEG)-modified electrospun membranes. In this study, poly( L -lactide-co-glycolide) (PLLGA)–PEG copolymer and pure PEG were blended with PLLGA, and then electrospun onto nonwoven membranes. The effects of blending of PLLGA–PEG or pure PEG on the adsorption of proteins, and further on the adhesion and proliferation of L929 fibroblasts on the electrospun membranes were investigated. Addition of PLLGA–PEG or PEG significantly improved the hydrophilicity of the electrospun membranes. Pure PEG had no obvious effects on the growth of L929 fibroblasts; in contrast, PLLGA–PEG significantly inhibited the adsorption of proteins and the proliferations of the cells on the electrospun membranes. In response to diminished protein adsorption, mRNA expression of genes related to cell adhesion and migration was up-regulated. The limited effects of pure PEG were probably caused by its preferential dissolution, whereas membrane-confined PLLGA–PEG displayed excellent performance on the inhibition of protein adsorption and cell proliferation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2897–2904, 2012.
研究领域:
生物医学工程
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 12
·1734-1743
·
IF 4.776
Shuhao Wang,Fei Zan,Yu Ke,Gang Wu
Introducing or grafting molecules onto biomaterial surfaces to regulate cell destination via biophysical cues is one of the important steps for biomaterial design in tissue engineering. Understanding how cells feel the substrate makes it easier to learn the mechanism behind cell–material interaction. In this study, on a glass substrate, we constructed poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths via a surface-induced atom transfer radical polymerization (SI-ATRP) method. FTIR-ATR and XPS tests of the formed polymer brushes indicate that these brushes have characteristic chemical structures of PHEMA; the polymer brush length revealed by the AFM tests increases linearly with reaction time. Cell lines of BMSCs, ATDC5, and human chondrocytes (HC) were cultured on these substrates to evaluate proliferation, adhesion, and differentiation. Our results demonstrated that the cells cultured on the substrates with short PHEMA brushes developed a spread morphology and organized actin fibers as compared to the cells cultured on those with long brushes. Different cell lines showed different responses depending on the PHEMA brush length. Cells cultured on long PHEMA brushes displayed a more rounded shape, higher gene expression of FAK and integrin, and lower gene expression of NCAM and N-cadherin as compared to those, especially ATDC5 cells, cultured on short PHEMA brushes. On PHEMA brushes with a long length, the cell lines express higher cartilage-specific genes including Sox9 and Col2 and GAG in ECM. The results suggest that polymer brushes having different lengths may interfere with the behavior of the cells cultured on them.
研究领域:
生物材料学
·
组织工程学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 39
·9525-9531
·
IF 7
Jie Li,Zhuang Ma,Anran Li,Siyuan Huang,Yufei Zhang,Yun Xue,Xianhui Song,Ye Zhang,Shihao Hong,Mo Wang,Zhongming Wu,Xinge Zhang
Microorganism adhesion and the resulting contamination of the biomaterial is one of the major causes of biomedical device failure. Stimuli-responsive materials based on dynamically regulating interactions with reversible characteristics of on–off states have attracted increasing attention. Here, a facile self-assembled biomaterial nanocoating constructed using acidity- and photoregulated spiropyran-modified nanoparticles was developed for reversibly regulating bacteria or mammalian cell adhesion-and-detachment. The coating was formed by coating a solution of spiropyran-conjugated nanoparticles around the surface of a silica gel followed by curing and drying at 60 °C for 30 min. Importantly, efficient adhesion-and-detachment of bacteria or cells could be controlled even after 8 cycles owing to the excellent acidity- and light-switched ability. Collectively, this well-defined self-assembled nanocoating as a dynamical and reversible agent provides promising insight for the development of biomedical devices, especially for biomaterial medical coatings.
研究领域:
生物材料学
·
表面工程
·
微生物学
·
再生医学
·
抗菌技术
✓ 已验证
DOI
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 13
·e2416771122
·
IF 9.1
Badri Narayanan Narasimhan,Stephanie I. Fraley
In native extracellular matrices (ECM), cells utilize matrix metalloproteinases (MMPs) to degrade and remodel their microenvironment. Accordingly, synthetic matrices have been engineered to permit MMP-mediated cleavage, facilitating cell spreading, migration, and interactions. However, the interplay between matrix degradability and mechanical properties remains underexplored. We hypothesized that MMP activity induces immediate mechanical alterations in the ECM, which are subsequently detected by cells. We observed that both fibrillar collagen and synthetic degradable matrices exhibit enhanced stress relaxation following MMP exposure. Cells responded to these variations in relaxation by modulating their spreading and focal adhesions. Furthermore, we demonstrated that stress relaxation and cell spreading can be precisely controlled through the rational design of matrix degradability. These findings establish a fundamental link between matrix degradability and stress relaxation, with potential implications for a broad spectrum of biological applications.
研究领域:
生物材料学
·
细胞生物学
·
生物力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
THIN SOLID FILMS
·
2014
·
IF 1.867
Kui Cheng,Xiaozhao Wang,Wenjian Weng,Jun Lin,Huiming Wang
Light-induced cell detachment on the substrate of TiO 2 nanodots has been proved to be a safe and attractive method for cell harvesting in tissue engineering. In order to improve the cell release efficiency, bioinert platinum with excellent charge transport loading in biocompatible graphite was prepared. 20 wt.% Pt/C powder showing excellent electrical, chemical and bioactive properties was incorporated to a TiO 2 system. We investigated the biocompatibility of Pt/C–TiO 2 composite films with different amounts of Pt/C with C/tetrabutyl titanate molar ratio varying from 0 to 0.42. The adhesion and proliferation rates of pre-osteoblastic MC3T3-E1 cells (mouse calvaria-derived) on the films prepared with the highest C/TiO 2 molar ratio are higher than that of the pure TiO 2 and other films, indicating a Pt/C dose-dependent effect. It is shown that the detachment efficiency of MC3T3-E1 cells depends on Pt/C amount and UV illumination time. Moreover, films prepared with C/tetrabutyl titanate molar ratio of 0.42 showed 93% cell detachment ratio within 5 min of UV illumination, while bare TiO 2 films only reached 75%, indicating an improved cell release efficiency by the incorporation of Pt/C. However, an interesting phenomenon was found that longer illumination time showed lower detachment ratios to some extent, which is contrary to our previous work. It is believed that this work presents the improved cell detachment efficiency by the incorporation of Pt/C.
研究领域:
生物材料学
·
组织工程学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Xinyu Hu,Yongmei Wang,Liangliang Zhang,Man Xu,Jianfa Zhang,Wei Dong
Salecan, a water-soluble extracellular β-glucan, has emerged as a promising candidate for preparing hydrogel biomaterials recently. Herein, photo cross linked composite hydrogels, composed by salecan/poly ( N-tert -butylacrylamide-co-acrylamide) network andα-Mo 2 C nanoparticles were prepared by ultraviolet (UV) light, using Irgacue 2959 as photo initiator. The XRD and EDX analysis illustrated the presence of embeddedα-Mo 2 C nanoparticles throughout the hydrogel matrix. Compression tests and thermal analysis revealed that the incorporation of α-Mo 2 C nanoparticles enhanced mechanical strength and thermal stability due to the structural reinforcement of the composite hydrogels. Moreover, the change of salecan content provided a mean to tailor both the swelling property and morphology of the composite hydrogels. Encapsulation of L929 and 3T3-L1 cells and the following viability test showed the excellent cell-compatibility of these hydrogels. Taken together, these studies support the use of photo crosslinked composite hydrogels as tunable biomaterials for cell adhesion.
研究领域:
生物材料学
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Hongmei Chen,Shu Yu,Yunping Li
SiOC and SiCaMgOC coatings were prepared by sol–gel method from precursors of hydrosilane and the mixture of hydrosilane, calcium acetylacetonate (Ca(acac) 2 ) and magnesium acetylacetonate (Mg(acac) 2 ), respectively. Fourier transform infra-red (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis on the coatings revealed that Ca 2+ and Mg 2+ ions can be successfully doped into SiOC coating after aging and annealing in the SiCaMgOC coating. The cytotoxicity and bioactivity of three samples have been detected by MTT assay, cell adhesion and simulated boy fluid (SBF) immersion test. The results suggested that SiCaMgOC coating induced more apatite than that of C/C and SiOC in the comparable soaking process. SiCaMgOC coating demonstrated better biological activity and cellular adherence for its higher surface roughness and ion exchange reaction between Ca 2+ and Mg 2+ ions and protons (H + /H 3 O + ) in the SBF solution.
研究领域:
材料科学与工程
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 4
·期 18
·9031-9038
·
IF 2.562
Delin Cheng,Xiaodong Cao,Huichang Gao,Xiaoling Ye,Wenxiu Li,Yingjun Wang
Composite polymer devices have gained considerable interest in biomedical applications. In this work, a composite poly(lactic-co-glycolic acid)/poly(ε-caprolactone) (PLGA/PCL) microsphere with a layered architecture and an island–sea topography was developed. PCL was found to occupy the shell layer of the microsphere, while PLGA constituted surface islands and the internal core. This subtle structure was derived from the different external and internal rates of phase separation between PCL and PLGA. The existence of the PLGA islands enhanced the hydrophilicity of the PCL shell. Mouse mesenchymal stem cells (mMSCs) were cultured and they grew well on the PLGA/PCL microspheres. The surface of the microsphere was microscopically composed of PCL sea and PLGA islands, and the mMSCs showed a trend to attach to the more hydrophilic PLGA islands and bridged between adjacent microspheres. This paper provides a new alternative to design functional microspheres for potential application in tissue repair.
研究领域:
生物医学工程
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2016
·
IF 3.42
Yanmei Li,Yanfang Wang,Jingjie Ye,Jiang Yuan,Yinghong Xiao
The natural abundance of cell adhesion sequences, RGD (Arg-Gly-Asp) and LDV (Leu-Asp-Val) in the keratins make them suitable as biomaterials for tissue engineering applications. Herein, keratins were coelectrospun with poly(ε-caprolactone)(PCL) at the ratio of 9/1, 8/2, and 7/3 to afford nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, SEM, WCA, and XPS. Cell attachment data showed that NIH 3T3 cells adhered more to the PCL/keratin nanofibrous mats than the pristine PCL mats. The MTT assay revealed that the PCL/keratin mats had improved cell viability. The blood clotting time test (APTT, PT, and TT) indicated the PCL/keratin mats exerted good blood compatibility. These mats would be a good candidate as a scaffold for vascular tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 18
·6692
·
IF 4.6
Hongcai Wang,Ruhong Yin,Xiuqiong Chen,Ting Wu,Yanan Bu,Huiqiong Yan,Qiang Lin
To enhance the mechanical strength and cell adhesion of alginate hydrogel, making it satisfy the requirements of an ideal tissue engineering scaffold, the grafting of Arg-Gly-Asp (RGD) polypeptide sequence onto the alginate molecular chain was conducted by oxidation of sodium periodate and subsequent reduction amination of 2-methylpyridine borane complex (2-PBC) to synthesize alginate dialdehyde grafted RGD derivatives (ADA-RGD) with good cellular affinity. The interpenetrating network (IPN) composite hydrogels of alginate/polyvinyl alcohol/cellulose nanocrystals (ALG/PVA/CNCs) were fabricated through a physical mixture of ion cross-linking of sodium alginate (SA) with hydroxyapatite/D-glucono-δ-lactone (HAP/GDL), and physical cross-linking of polyvinyl alcohol (PVA) by a freezing/thawing method, using cellulose nanocrystals (CNCs) as the reinforcement agent. The effects of the addition of CNCs and different contents of PVA on the morphology, thermal stability, mechanical properties, swelling, biodegradability, and cell compatibility of the IPN composite hydrogels were investigated, and the effect of RGD grafting on the biological properties of the IPN composite hydrogels was also studied. The resultant IPN ALG/PVA/CNCs composite hydrogels exhibited good pore structure and regular 3D morphology, whose pore size and porosity could be regulated by adjusting PVA content and the addition of CNCs. By increasing the PVA content, the number of physical cross-linking points in PVA increased, resulting in greater stress support for the IPN composite hydrogels of ALG/PVA/CNCs and consequently improving their mechanical characteristics. The creation of the IPN ALG/PVA/CNCs composite hydrogels’ physical cross-linking network through intramolecular or intermolecular hydrogen bonding led to improved thermal resistance and reduced swelling and biodegradation rate. Conversely, the ADA-RGD/PVA/CNCs IPN composite hydrogels exhibited a quicker degradation rate, attributed to the elimination of ADA-RGD by alkali. The results of the in vitro cytocompatibility showed that ALG/0.5PVA/0.3%CNCs and ADA-RGD/PVA/0.3%CNCs composite hydrogels showed better proliferative activity in comparison with other composite hydrogels, while ALG/PVA/0.3%CNCs and ADA-RGD/PVA/0.3%CNCs composite hydrogels displayed obvious proliferation effects, indicating that PVA, CNCs, and ADA-RGD with good biocompatibility were conducive to cell proliferation and differentiation for the IPN composite hydrogels.Keywords:alginate;interpenetrating network composite hydrogels;cellular adhesion;cellular adhesion;tissue engineering
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 3
·511-516
·
IF 4.872
Yifan Wang,Ying Xiao,Guorong Gao,Jing Chen,Ruixia Hou,Qiang Wang,Li Liu,Jun Fu
Graphene composite hydrogels have wide potential applications as biomaterials. Herein, L-cysteine was used to reduce graphene oxide (GO) into hydrogels. Systematic investigations by FTIR, Raman spectroscopy, TEM, and other methods reveal that the reduced graphene oxide (rGO) nanosheets are likely bridged by L-cysteine molecules, forming porous hydrogels with the rGO nanosheets stacked into layered structures on the walls. Such layered structures, as well as the reduction, are critical for the improvement of conductivity by four to five orders of magnitude in comparison to graphene oxide. In vitro cell culture experiments demonstrate excellent cell adhesion and growth on these reduced graphene oxide hydrogels. These conductive rGO hydrogels may find applications in electrical stimulation to facilitate cell adhesion and growth.
研究领域:
生物材料学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 18
·10650-10656
·
IF 6.739
Jie Lu,Fuyin Zheng,Yao Cheng,Haibo Ding,Yuanjin Zhao,Zhongze Gu
Cell adhesion and alignment are two important considerations in tissue engineering applications as they can regulate the subsequent cell proliferation activity and differentiation program. Although many effects have been applied to regulate the adhesion or alignment of cells by using physical and chemical methods, it is still a challenge to regulate these cell behaviors simultaneously. Here, we present novel substrates with tunable nanoscale patterned structures for regulating the adhesion and alignment of cells. The substrates with different degrees of pattern orientation were achieved by customizing the amount of stretching applied to polymer inverse opal films. Cells cultured on these substrates showed an adjustable morphology and alignment. Moreover, soft hydrogels, which have poor plasticity and are difficult to cast into patterned structures, were applied to infiltrate the inverse opal structure. We demonstrated that the adhesion ratio of cells could be regulated by these hybrid substrates, as well as adjusting the cell morphology and alignment. These features of functional inverse opal substrates make them suitable for important applications in tissue engineering.
研究领域:
生物材料学
·
细胞生物学
·
组织工程学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）