MICROCHIMICA ACTA
·
2010
·Vol. 171
·期 3
·385-392
·
IF 2.648
Yin Huanshun,Zhou Yunlei,Liu Tao,Cui Lin,Ai Shiyun,Qiu Yanyan,Zhu Lusheng
An amperometric biosensor for nitrite was prepared by immobilizing cytochrome c (Cyt c) on a gold electrode that was modified with Nafion and a Cu-Mg-Al layered double hydroxide (Cu-LDH). The Cu-LDH was characterized by Fourier transform infrared spectroscopy and powder X-ray diffraction. The UV-visible spectrum suggests that Cyt c retains its native conformation in the modified film. The direct electrochemical investigation indicated that the composite film represents a good platform for the immobilization of Cyt c as well as an excellent promoter for the electron transfer between Cyt c and the gold electrode. Moreover, the biosensor showed a remarkable bioelectrocatalytic activity for the oxidation of nitrite with a linear range from 0.75 to 123 μM. The detection limit is 2 × 10 −7 M (S/ N = 3). The biosensor was successfully applied to the determination of nitrite in food samples.
研究领域:
生物分析化学
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电化学传感
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食品分析
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生物传感器
·
分子诊断
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ANTI-CANCER DRUGS
·
2019
·Vol. 30
·期 1
·46-55
·
IF 1.801
Lin Fuxing,Yang Jie,Muhammad Umair,Sun Jing,Huang Zhenghua,Li Wei,Lv Fengxia,Lu Zhaoxin
Bacillomycin D can inhibit the growth of Aspergillus ochraceus in food samples. In addition, it can induce apoptosis in and inhibit the proliferation of cancer cells, although the details of this mechanism are unknown. In this study, we separated bacillomycin D-C14, D-C15, D-C16
monomers from the Bacillus subtilis strain fmbJ. The bacillomycin D monomers containing longer fatty acid chains better induced apoptosis in Bgc-823, Sgc-7901, and Hgc-27 gastric cancer cells. The Bgc-823 cell line was the most sensitive. Acridine orange-ethidium bromide staining indicated
that bacillomycin D-C16-induced Bgc-823 cell death by triggering apoptosis, characterized by membrane blebbing, cellular shrinkage, and DNA fragmentation. Flow cytometric analysis showed a bacillomycin D-C16 dose-dependent trigger of Bgc-823 apoptosis. Bacillomycin D-C16-induced the mitochondrial
pathway, as indicated by a reduced Bcl-2/Bax expression ratio, enhanced cytochrome C release, and higher levels of cleaved caspase-3. Furthermore, bacillomycin D-C16 effectively repressed phosphorylation of the serine–threonine protein kinase Akt at Ser-473 and increased the levels of
the FoxO3a protein. The combination of the PI3K/Akt-inhibitor BEZ235 with bacillomycin D-C16 enhanced the apoptosis of Bgc-823 cells. Together, these findings indicated that bacillomycin D-C16 induces apoptosis through the PI3K/Akt and FoxO3a signaling pathways.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
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药理学
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ziyi Yang,Yihang Li,Guanxia Qiu,Qi Xu,Zhen Wu,Xiuhua Zhang,Shengfu Wang,Wei Wen
An antifouling nanosensor was constructed with a thin-layer hydrogel interface for effectively resisting non-specific adsorption. • Gel/Pt/CFE achieved ultrasensitive and accurate detection of H 2 O 2 with a low LOD of 1.093 μM. • The nanosensing platform enabled spatiotemporal-resolution monitoring of H 2 O 2 levels in PC-12 cells at the single-cell level. Accurate detection of endogenous hydrogen peroxide (H 2 O 2 ) at the single-cell level is crucial for elucidating its important roles in cellular signaling and oxidative stress. However, the accuracy of nanoelectrochemical sensors is severely hindered by non-specific adsorption at the sensing interface in complex cellular environments. The adsorption of proteins and other biomolecules can significantly passivate the electrode interface, leading to signal shifts and unreliable monitoring. Herein, an innovative implantable nanosensor with robust antifouling interface was developed by electrodepositing platinum nanoparticles onto a carbon fiber electrode, followed by the in-situ synthesis of hydrophilic poly(ethylene glycol) diacrylate hydrogel layer. The hydrogel layer served as a highly effective diffusion barrier against non-specific adsorption of proteins and other interferents while remaining permeable to H 2 O 2 molecules. The results demonstrated that the proposed nanosensor had excellent sensitivity and stability for H 2 O 2 detection ranging from 10 to 1000 μM, with a detection limit of 1.093 μM (S/N = 3), enabling in-situ monitoring of intracellular H 2 O 2 fluctuations in single cells with high accuracy. This work provides a reliable platform for nanoelectrochemical sensing toward small biomolecules in complex biological matrices. Download: Download high-res image (209KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Ala'a Mhmoued Abdllh Alboull,Yu Zheng,Saman Hosseinkhani,Mohamed Ibrahim Halawa,Altaf Hussain,Jiyang Liu,Guobao Xu
Zn–BBPY–MOG was synthesized via simple ambient self-assembly. • Zn–BBPY–MOG served as a self-luminescent ECL platform. • Zn–BBPY–MOG/S₂O₈ 2− achieved 81.5% relative ECL efficiency. • Cyt c was detected with a low LOD of 0.025 nM. Electrochemiluminescence (ECL) sensing has emerged as a powerful analytical technique for biomolecule detection. Cytochrome c (Cyt c), an important biomarker associated with cellular damage and various diseases, has attracted considerable attention in bioanalytical research. Herein, we report a zinc-based metal-organic gel (Zn-BBPY-MOG) as a self-luminescent ECL platform for the sensitive detection of Cyt c. Zn-BBPY-MOG was synthesized under ambient conditions via coordination between Zn 2+ ions and the π-conjugated ligand 2,6-bis(2-benzimidazolyl)pyridine (BBPY). The resulting gel network, featuring strong coordination interactions and an extended π-conjugation, facilitates efficient electron transfer and enables intense ECL emission in the presence of S₂O₈ 2− as a coreactant. Compared with the standard Ru(bpy)₃ 2+ /S₂O₈ 2− reference system, Zn-BBPY-MOG/S₂O₈ 2− achieved a relative ECL efficiency of 81.5%, demonstrating its efficient ECL performance. Based on the quenching effect of Cyt c on the Zn-BBPY-MOG/S₂O₈ 2− ECL system, a sensitive and quantitative detection strategy was established. The developed sensing platform exhibited a wide linear detection range from 1 to 1000 nM and an ultralow detection limit of 0.025 nM for Cyt c. Moreover, the proposed method was successfully applied to the determination of Cyt c in serum samples, achieving satisfactory recoveries ranging from 99.20% to 102.50%. These results demonstrate the reliability and practical applicability of the Zn-BBPY-MOG-based ECL platform for sensitive biomolecular analysis. Download: Download high-res image (87KB) Download: Download full-size image
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Innovative Food Science & Emerging Technologies
·
2026
·
IF 6.9
Minbo Li,Xianbing Xu,Min Fan,Junjie Yi,Ming Du
Clean-label clarification of polysaccharide-rich mushroom hydrolysates is critical for producing high-quality natural seasonings. In this study, we developed a green polysaccharide-targeted clarification strategy termed cryo-induced molecular flocculation (CIMF), which offers a remarkable advantage of being additive-free and highly efficient compared with conventional clarification methods such as natural sedimentation, ethanol-assisted flocculation, and chitosan-assisted flocculation for clarifying Termitomyces albuminosus hydrolysates (TAH). CIMF achieved a high clarification efficiency (60.41%) without the use of external flocculants by selectively aggregating and removing insoluble polysaccharides (41.25%) while efficiently retaining peptides (96.15%). Structural analyses and molecular dynamics simulations further revealed that low temperature facilitated monosaccharide aggregation by reducing the solvent-accessible surface area and enhancing sugar–sugar interactions leading to compact cluster formation. In summary, CIMF represents a clean-label and polysaccharide-oriented clarification strategy that provides a sustainable and green approach for the manufacture of high-quality functional food ingredients.
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Materials Today Bio
·
2025
·
IF 10.2
Mingcheng Wang,Huixi Yi,Qibao Zheng,Muhammad Adnan Younis,Liyou Guo,Zhixiong Zhan,Muhammad Rizwan Younis,Chengzhi Jin,Dong-Yang Zhang
The high metastatic rate of breast tumor is the prominent reason of its poor prognosis, while the inflammatory microenvironment of tumor tissues further promoted tumor metastasis. Although photothermal therapy (PTT) displays high antitumor efficacy, the rise of inflammation-induced reactive oxygen species (ROS) during PTT exacerbate tumor metastasis. To prevent breast tumor metastasis and relieve inflammation-induced oxidative stress during PTT, herein, we developed self-assembled nanodrugs (FCP) consisting of carminic acid, iron ion, and polyvinylpyrrolidone, demonstrating photoacoustic imaging-guided PTT and anti-inflammatory activity to restrict the growth of both primary breast tumor and metastatic tumor. The as designed self-assembled spherical FCP nanoparticles (NPs, 41 nm) exhibited good light to heat conversion and broad-spectrum multienzyme (superoxide dismutase, etc.) mimetic activity to scavenge excess ROS and alleviate oxidative stress. Meanwhile, FCP NPs showed a positive correlation between the photothermal heating and ROS scavenging, allowing the continuous consumption of ROS during PTT. Importantly, owing to the intrinsic bimodal photothermal and photoacoustic imaging abilities, FCP NPs effectively guided and monitored the in vivo treatment process, which facilitated to restrict the growth of both primary and metastatic breast tumor in vivo due to coordinated PTT and anti-inflammation as confirmed by TUNEL, ki67, and matrix metalloproteinase-9 stainings. Whereas FCP NPs did not pose any potential damage to the vital organs, presenting good biosafety in vivo . We envision that self-assembled nanodrugs with concurrent anti-inflammatory and photothermal activities may have great clinical prospects in the treatment of metastatic cancers.
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ACS Applied Polymer Materials
·
2025
·
IF 4.7
Huanping Wu,Xiaoqiang Wu,Xuyang Liu,Zulpiyam Ahmatjan,He Teng,Chenchen Zhang,Jianlong Ye,Jianqiang Meng
The preparation of molecularly imprinted polymers by simple and universally applicable methods for the rapid and efficient purification of proteins has always been a significant challenge within the realm of biological separation. In this work, interconnected porous molecularly imprinted polyampholyte hydrogels (IPMIPs) were prepared by one-step free radical polymerization of monomers bearing opposite charges by using high internal phase emulsions as templates for lysozyme (Lyz) separation. By changing the concentration of surfactant Tween 20 and hydrophilic silica nanoparticles N20, the pore interconnectivity and pore size of the imprinted hydrogels could be finely adjusted, thereby affecting the swelling process. Owing to the well-defined and interconnected porous structure, IPMIPs were capable of achieving adsorption equilibrium within a remarkably short time frame of 45 min. The monolayer adsorption capacities of IPMIPs for Lyz reached an impressive 242.5 mg/g with an imprinting factor (IF) of 3.01 for Lyz. Through electrostatic complementation and hydrogen bond interactions, IPMIPs showed Lyz adsorption capacities significantly higher than those of other reference proteins, and the selectivity coefficient (α) to ovalbumin (OVA) was as high as 7.17. The real sample separation and regeneration experiments further demonstrated the excellent specific recognition ability and reliability of the IPMIPs. This strategy presents a highly promising alternative for the Lyz separation process and has the potential to be extended to other protein molecules.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Wenqi Zhang,Houyi Peng,Chen Wang,Xiaohui Ju,Weixing Li
Peptides are produced through protein hydrolysis and typically contain many products of different molecular weights. In order to obtain peptide products within a specific molecular weight range, different membranes are generally used in the purification process. Here, a smart nanofiltration (NF) membrane with specific pore size under different pH conditions was proposed to separate soybean peptides. Carboxymethyl chitosan (CMCS) was introduced into the interfacial polymerization of polyamide membrane. The abundant amine groups on CMCS enabled pH-dependent conformational changes through protonation/deprotonation, thus tuning membrane surface charge and effective pore size by modulating pH of feed solution. The developed NF membrane showed that the rejection of thymopentin (679 Da) was 98.54% at pH 12 but only 40.84% at pH 3. When sequential filtrating the enzymatic hydrolysis solution at pH 12 and 3, the mass ratio of permeate soybean peptides in 189–1450 Da arrived at from 52.93% to 89.35%. The developed smart membrane simultaneously removed both large molecules and small amino acids to extract the objective peptides. A groundbreaking approach to purify biomolecules with one membrane was proposed, which would be prospective for shortening refinement processes.
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TALANTA
·
2026
·
IF 6.7
Jingjing Yin,Yuehua Wang,Shanshan Zhu,Xiaoxue Liu,Yujie Sun,Hong Yang,Yifei Ma,Jinhua Liu
The development of wavelength-tunable fluorescence immunoassays for sensitive biomarker detection remains a significant challenge in clinical diagnostics. In this work, we present an HRP-induced in situ fluorogenic immunoassay platform based on the rapid reaction between p-phenylenediamine (PPD) analogues and 6-hydroxyquinoline (6-Hy). The proposed mechanism involves HRP-catalyzed generation of hydroxyl radicals from H 2 O 2 , which subsequently oxidize PPD to form fluorescent copolymer nanoparticles (FCNPs) with 6-Hy. By strategically modifying the PPD structure with various substituents, we achieved discrete and tunable emission of FCNPs, with maxima spanning from 380 to 600 nm (Δλ ≈ 220 nm). Using cardiac troponin I (cTnI) as a model antigen and zinc-coordination nanoparticles (ZnNPs) as carriers, the immunoassay demonstrated exceptional performance, with a wide dynamic range (0.5-125 ng/mL) and an ultralow detection limit (0.17 ng/mL) for cTnI. The platform was successfully validated in human serum samples, showing excellent correlation with clinical standards. This work not only establishes a novel strategy for wavelength-programmable immunoassays but also provides a versatile platform for advancing biomarker detection in clinical diagnostics.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 12.5
Yuxuan Yang,Sufeng Zhang,Lijing Zhang,Nan Zhang,Liwei Qian,Wenqi Song
To address the low binding-site density and mass-transfer limitations of conventional protein-molecularly imprinted polymers (P-MIPs), this study proposes a synergistic strategy combining a custom-synthesized imidazolium ionic liquid monomer, 1-vinyl-3-diacetamidoimidazolium chloride ([VDAIM]Cl), with a bacterial-cellulose@UiO-66 (BC@UiO-66) hybrid scaffold. Spectral characterization and molecular docking simulations revealed multiple strong interactions between [VDAIM]Cl and bovine serum albumin (BSA), enhancing the stability of the pre-polymer complex compared to conventional monomers. Meanwhile, benefiting from the structural advantages of BC, the template immobilization-surface imprinting strategy (TIS- co -SIT) was employed to construct a high-affinity imprinted membrane. The resulting BC@UiO-66@MIPs exhibited fast adsorption equilibrium within 50 min, a large binding capacity of 502.5 ± 27 ( n = 3) mg g −1 , and a high imprinting factor ( IF ) of 6.27 ± 0.84 (n = 3). Furthermore, BC@UiO-66@MIPs also demonstrated excellent reusability, retaining over 90% of its initial binding capacity after ten adsorption-desorption cycles. Notably, the materials exhibited outstanding selectivity, with a competitive adsorption factor ( α ) of 5.57 ± 0.62 ( n = 3) in binary BSA/BHb mixtures, and a high permeation selectivity coefficient ( β ) of 6.37. The membrane successfully isolated BSA from complex calf serum, confirming its practical potential for protein purification. This study constructs an efficient molecular imprinting system by integrating rational monomer design with advanced porous supports to enhance protein recognition and purification.
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JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 7
·e70479
·
IF 3.6
Qinghua Cao,Shuai Lv,Yue Wang,Xijie Wang,Hailin Cong,Bing Yu
Zwitterionic compounds exhibit strong hydrophilicity, act as hydrogen bond receptors and donors, and form robust hydration layers through ionic solvation, which effectively prevent unwanted protein adsorption. To reduce nonspecific interactions between analytes and the inner surface of the separation compartment and then improve detection sensitivity and separation efficiency, it is essential to modify fused silica capillaries with functional groups. In this study, we synthesized a zwitterionic antifouling peptide (Glu-Lys) 3 Glu using solid-phase peptide synthesis. Then, we bound the peptide to the inner surface of a fused silica capillary using diazo resin as a coupling agent, resulting in stable and excellent antifouling and separation abilities. To evaluate the properties of the coating, we conducted fluorescent protein adsorption and electrophoresis experiments. The obtained results demonstrated that the coating effectively prevented protein adsorption and made it possible to separate five standard proteins in 20 min. Moreover, three proteins of egg white were separated within 15 min. These findings confirmed the very good anti-protein adsorption performance of the peptide-coated capillary and its practical value for various applications.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Shulong Cai,Liqi Fan,Dan Liu,Changxing Wei,Na Yang,Xichao Fu,Rong Tang,Jucai Xu,Sheng Yin,Xin Chen
Alcalase and neutral protease are widely employed for producing soybean antioxidant peptides, but their synergy mechanisms remain unclear. In this work, soybean protein isolate (SPI) was hydrolyzed synergistically by the two proteases. The products by Alcalase exhibited a larger proportion of low molecular-weight (MW, <1 kDa) peptides compared to those from neutral protease (87.74 % vs 76.08 %). After synergistic hydrolysis, an “averaging” effect on free amino acid composition and MW distribution was observed, but the peptide length distribution shifted evidently towards dipeptides (57.07 % with simultaneous enzyme use, compared to 20.43 % and 33.70 % for individual enzymes). Similarly, the N/C-terminal residue distribution also displayed an ‘averaging’ trend. Furthermore, synergistic processing increased the proportion (approximately 7 %) of potential antioxidant peptides with predicted probability >0.7, aligning with observed non-additive antioxidant activities and suggesting a synergistic mechanism beyond simple additivity. We hope the work could provide guidance and references for the target peptide release from SPI.
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