Owing to the unpredictable size of wounds and irregular edges formed by trauma, nanofibers' highly customizable and adherent in situ deposition can contribute to intervention in the healing process. However, electrospinning is limited by the constraints of conventional polymeric materials despite its potential for anti-inflammatory and antimicrobial properties. Here, inspired by the Janus structure and biochemistry of nanometal ions, we developed an in situ sprayed electrospinning method to overcome bacterial infections and immune imbalances during wound healing. The bilayer fiber scaffold has a hydrophobic outer layer composed of polycaprolactone (PCL) and a hydrophilic inner layer composed of gelatin, poly (L-lactic acid) (PLLA), and magnesium oxide nanoparticles (MgO NPs), constituting the PCL/PLLA-Gelatin-MgO (PPGM) electrospun scaffold. This electrospun scaffold blocked the colonization and growth of bacteria and remained stable on the wound for continuous anti-inflammatory properties to promote wound healing. Furthermore, PPGM electrospinning modulated collagen deposition and the inflammatory microenvironment in the full-thickness skin model, significantly accelerating vascularization and epithelialization progression. This personalized Janus electrospun scaffold has excellent potential as a new type of wound dressing for first aid and wound healthcare.
Xinglei Li,Yiyang Shen,Shengye You,Luxuan Shao,Rui Bao,Diya Zhang,Wei Dong,Jun Lin
Available remedies for diabetic oral mucosa wounds often prove inadequate, chiefly due to complications like bacterial infiltration, oxidative harm, and hindered angiogenesis throughout wound restoration. This study addresses the significant gap in effective therapeutic options by developing an injectable hydrogel, DCE1, designed to enhance healing in diabetic oral ulcers. Here, we present DCE1, composed of a polysaccharide matrix of dialdehyde xanthan gum and quaternary chitosan, integrated with epigallocatechin-3-gallate nanoparticles. DCE1 generates a shielding hydrogel barrier when administered locally, exhibiting potent sterilization and reactive oxygen species-clearing properties. Such benefits shift the wound healing process from an inflammatory to a proliferative stage. Notably, the DCE1 dressing is fully biodegradable post-treatment, ensuring complete absorption by the body after fulfilling its therapeutic role. In conclusion, our findings demonstrate that the DCE1 hydrogel significantly accelerates oral mucosa wound repair in both in vitro and in vivo models. This advancement represents a promising therapeutic strategy for diabetic oral ulcers, ultimately contributing to improved clinical outcomes in the management of this challenging condition.
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