SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinxin Wang,Shan Sun,Ke Cheng,Tao Lin,Henggang Wang,Haoyi Sun,Hengwei Lin,Peng Wang
Pathogenic bacterial spores pose a considerable threat to human health due to their ability to survive extreme environmental conditions. Thus, sensitive detection and efficient inactivation of these spores are essential for preventing disease transmission. However, most existing studies have predominantly concentrated on detection alone. Herein, we developed a versatile nanoplatform that integrates sensitive bacterial spore sensing and in situ spore inactivation capabilities by assembling terbium ions (Tb 3+ ) with photosensitizing carbon dots (CDs), referred to as Tb-CDs nanoassemblies (NAs). By leveraging the unique optical properties of CDs and Tb 3+ ions, along with the antenna effect of lanthanides, Tb-CDs NAs demonstrated highly sensitive visual detection of the key bacterial spore biomarker, pyridinedicarboxylic acid (DPA), achieving a low detection limit of 0.68 μM and excellent selectivity. Additionally, Tb-CDs NAs exhibited practical sensing capabilities in various environments, including spore suspensions and real samples, while monitoring bacterial spore germination. Further studies confirmed that the DPA-triggered disassembly of Tb-CDs NAs led to the release of CDs, enabling higher affinity for spores and in situ inactivation through singlet oxygen generation. This work underscores the potential of CDs-based assemblies for simultaneous pathogen detection and inactivation, providing promising tools for preventing bacterial spread and contamination.
研究领域:
生物传感
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微生物学
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纳米医学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhuoya Hao,Jiacheng Zhang,Qin Peng,Xuefeng Tian,Jun Zhang,Jiayi Ai,Qiqi Jia,Yihui Yuan,Ning Wang
The increasing global reliance on nuclear energy highlights the critical need for uranium recovery from nuclear wastewater. However, due to the harsh environment of concentrated acid and nuclear radiation, the development of new adsorbent for uranium recovery from nuclear wastewater is highly needed. The bacterial spore has been reported to exhibit high tolerance to these harsh environments and contain functional components with reliable uranium binding ability, while there is still no report on the use of bacterial spore for uranium recovery from nuclear wastewater. Herein, the potential of the bacterial spore for being used in uranium recovery was analyzed for the first time. The result shows that the bacterial spore realizes a high uranium adsorption capacity of 245 mg g −1 in uranium-containing aqueous environment. With a low dosage of 0.1 g L −1 , the bacterial spore achieves a high uranium removal performance of 99.03 % in simulated nuclear wastewater. Additionally, when the concentration of HNO 3 reached 12 M, the adsorption capacity can still reach 156 mg g −1 after 24 h of treatment, proving its high tolerance to concentrated acid. Moreover, after being irradiated by γ-ray with the radiation dosage of 500 kGy, the bacterial spore retains 95.94 % of the uranium adsorption capacity, indicating its high resistance to nuclear radiation. These findings indicate that the bacterial spore would be a highly potential adsorbent for the recovery of uranium from nuclear wastewater with concentrated acid and nuclear radiation.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 12
·1-13
·
IF 5.7
Zhu Yaodi,Tian Jiaqi,Li Miaoyun,Zhao Lijun,Shi Jiyong,Liu Weijia,Liu Shijie,Liang Dong,Zhao Gaiming,Xu Lina,Yang Shufeng
A new surface-enhanced Raman spectroscopy (SERS) biosensor of Graphene@Ag-MLF composite structure has been fabricated by loading AgNPs on graphene films. The response of the biosensor is based on plasmonic sensing. The results showed that the enhancement factor of three different spores reached 10 7 based on the Graphene@Ag-MLF substrate. In addition, the SERS performance was stable, with good reproducibility (RSD<3%). Multivariate statistical analysis and chemometrics were used to distinguish different spores. The accumulated variance contribution rate was up to 96.35% for the top three PCs, while HCA results revealed that the spectra were differentiated completely. Based on optimal principal components, chemometrics of KNN and LS-SVM were applied to construct a model for rapid qualitative identification of different spores, of which the prediction set and training set of LS-SVM achieved 100%. Finally, based on the Graphene@Ag-MLF substrate, the LOD of three different spores was lower than 10 2 CFU/mL. Hence, this novel Graphene@Ag-MLF SERS substrate sensor was rapid, sensitive, and stable in detecting spores, providing strong technical support for the application of SERS technology in food safety. Graphical abstract
研究领域:
分析化学
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纳米技术
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生物传感器
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食品安全
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光谱学
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化学计量学
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2023
·
IF 6.2
Huijun Li,Xin Xu,Yonghui Liu,Yanan Hao,Zhouqing Xu
Developing multifunctional luminescent switches for detecting various poisons are urgent given the continuous deterioration of the environment. Herein, a new porous fluorescent metal–organic framework [Tb 2 (TPTC)(DMA) 4 (NO 3 ) 2 ] n (HPU-25) was initially designed. Then, fluorophore molecule tris(2,2′-bipyridine) dichlororuthenium(II) hexahydrate (Ru(bpy) 3 2+ ) was loaded onto HPU-25 by a simple post-synthesis strategy to prepare a new ratio fluorescence sensor Ru(II)@HPU-25 . As a sensor, it can detect 2,6-pyridinedicarboxylic acid (DPA) content in real-time, with high sensitivity and selectivity, without being disturbed by probe concentration and environment. Evident changes in fluorescence color and intensity are observed in distinguishing DPA contents. In addition, after detecting DPA, it can be used for consecutive visual detection of dichromate anion (Cr 2 O 7 2− ) in water. This multifunctional fluorescence sensor could make up for the defects of single fluorescence and single function of the traditional fluorescence sensor by using the dual strategies of ratio sensing and cascade sensing. Interestingly, DPA and Cr 2 O 7 2− in samples can be visually detected rapidly in real-time through the chroma acquisition APP on smartphone. More importantly, we also designed the combination of two logic gate operations for sensing the two targets. In the poison detection in daily life, these intelligent applications of fluorescence sensors have a broad development space.
研究领域:
材料科学
·
分析化学
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环境科学
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纳米技术
·
传感器技术
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产品关联
DOI
高影响因子
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