MACROMOLECULAR MATERIALS AND ENGINEERING
·
2018
·Vol. 303
·期 5
·1700590
·
IF 2.69
Qian Wang,Yunbo Feng,Min He,Yanping Huang,Weifeng Zhao,Changsheng Zhao
Herein, ene-functionalized dopamine is first coated onto a polyether sulfone membrane surface to introduce double bonds as anchoring sites for hydrogel film. Then, a hydrogel film is synthesized and simultaneously attached onto the membrane via photoinduced crosslinking copolymerization of N -isopropylacrylamide (NIPAAm) and methacryloxyethyltrimethyl ammonium chloride. The thickness of the hydrogel film can be well-controlled ranging from the nanometer to micrometer scales, which can retain the permeability of the membrane. Water contact angle and swelling tests demonstrate that the modified membrane shows thermoresponsive surface wettability. The quaternary ammonium salts in the hydrogel film can kill the attached bacteria efficiently and the dead bacteria can be detached by reducing the temperature below the lower critical solution temperature of poly(NIPAAm). The modified membrane also shows improved hemocompatibility, confirmed by prolonged clotting time and low hemolysis ratio. Hence, the designed thermoresponsive antibacterial hydrogel film with enhanced hemocompatibility has great potential to be applied in biomedical areas.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
POLYMER SCIENCE SERIES B
·
2023
·Vol. 64
·期 6
·897-905
·
IF 1.2
Li Yang,Liu Jun,Mai Chongyang,Lv Chengcheng,Guo Yawen,Zhao Lielun,Jiang Yan,Zhang Hongwen
Abstract In this study, the macroinitiator poly(hexafluorobutyl methacrylate) (PHFBMA) was first prepared by atom transfer radical polymerization (ATRP) method, and then PHFBMA- b -PHEMA block copolymer was prepared by ATRP method using hydroxyethyl methacrylate (HEMA) as the monomer. After that, the amphiphilic diblock copolymer was used to construct a porous membrane using breath figures. Surface hydrophobicity is one of the important properties of materials. It is inspired by the principle of nepenthes predation, that the slippery liquid-infused porous surface (SLIPS) mimics the inner wall structure of the pitcher plant. In order to enhance the surface hydrophobicity of the fluoropolymer coating, the silicone oil infused on the ultra-slip surface is innovatively modified by nano-SiO 2 filling. When the content of polysiloxane is unchanged (10 mL for silicone oil and 0.5 g for polysiloxane), the modified silicone oil filled with 1.5 g of nano-SiO 2 owns the best modification effect at the film concentration of 37.5 mg/mL. The results show that the hydrophobic properties of the SLIPS are greatly improved compared with the porous film. The water contact angle of SLIPS is 160 o , the sliding angle of SLIPS is 7°. In addition, due to the presence of surface silicone oil, the SLIPS has good resistance to bacterial adhesion.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 52
·46270-46277
·
IF 3.289
Wei Shao,Jimin Wu,Hui Liu,Guanghui Dong,Shuxia Wang,Huihua Min,Min Huang
Bacterial adhesion on the surfaces of medical devices, food processing equipment, heat exchangers and ship hulls has been recognized as a widespread problem. Bacterial adhesion on a surface is influenced by surface physical and chemical properties of the surface. In this paper, polyethyleneimine (PEI) grafted graphene oxide (GO) nanosheets were incorporated into Ni–P coatings by an electroless plating technique to modify the surface energy, and therefore to influence the interactions between the prepared surfaces and the tested bacteria. Ni–P–GO coatings were investigated by SEM, AFM, Raman spectrum and XPS. Contact angles of Ni–P–GO coatings with different GO loadings were tested and the surface free energies and their dispersive and polar components were calculated using the van Oss acid–base approach. The results showed that the surface free energies of the coatings had a significant influence on S. aureus adhesion. The Ni–P–GO coatings showed excellent antibacterial adhesion activity. Extended DLVO theory is used to explain the antibacterial adhesion behavior. The novel coatings reported here can be used in controlling bacterial adhesion and biofilm formation for various applications.
研究领域:
材料科学
·
微生物学
✓ 已验证
DOI
高影响因子
Acta Biomaterialia
·
2019
·
IF 6.638
Yuhui Lu,Yanan Wang,Jieyu Zhang,Xuefeng Hu,Zeyu Yang,Yi Guo,Yunbing Wang
Electrical stimulation (ES) via electrodes is promising for treating chronic wounds, but this electrode-based strategy is unable to stimulate the whole wound area and the therapeutic outcome may be compromised. In this study, a conductive poly(2-hydroxyethyl methacrylate) (polyHEMA)/polypyrrole (PPY) hydrogel was developed, and 3-sulfopropyl methacrylate was covalently incorporated in the hydrogel’s network to in-situ dope the PPY and maintain the hydrogel’s conductivity in the weak alkaline physiological environment. The obtained hydrogel was superior to the commercial Hydrosorb® dressing for preventing bacterial adhesion and protein absorption, and this is helpful to reduce the possibilities of infection and secondary damage during dressing replacement. The in vitro scratch assay demonstrates that ES through the hydrogel enhanced fibroblast migration, and this enhancement effect remained even after the ES was ended. The in vivo assay using diabetic rats shows that when ES was conducted with this polyHEMA/PPY hydrogel, the healing rate was faster than that achieved by the electrode-based ES strategy. Therefore, this polyHEMA/PPY hydrogel shows a great potential for developing the next generation of ES treatment for chronic wounds. Statement of Significance Electrical stimulation (ES) via separated electrodes is promising for treating chronic wounds, but this electrode-based strategy is unable to stimulate the whole wound area, compromising the therapeutic outcome. Herein, a hydrogel was developed with stable electrical conductivity in the physiological environment and strong resistance to protein absorption and bacterial adhesion. The in vitro and in vivo tests proved that ES applied through the flexible and conductive hydrogel that covered the wound was superior to ES through electrodes for promoting the healing of the chronic wound. This hydrogel-based ES strategy combines the advantages of ES and hydrogel dressing and will pave the way for the next generation of ES treatment for chronic wounds.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Shanshan Yang,Yichao Wu,Chenchen Qu,Jeremy B. Fein,Yizhuang He,Qiaoyun Huang,Peng Cai
Cyclic diguanylate monophosphate (c-di-GMP) is a ubiquitous intracellular secondary messenger which governs the transition from a bacterial cell’s planktonic state to biofilm formation by stimulating the production of a variety of exopolysaccharide material by the bacterial cell. A range of genes involved in c-di-GMP signaling in the Gram-negative species Comamonas testosteroni have been identified previously, yet the physical-chemical properties of the produced extracellular polymeric substances (EPS) and the bacterial adhesion characteristics regulated by c-di-GMP are not well understood. Here, we modulated the in vivo c-di-GMP levels of Comamonas testosteroni WDL7 through diguanylate cyclase (YedQ) and phosphodiesterase (YhjH) gene editing. The strains and their adhesion properties were characterized by Fourier-transform infrared and two-dimensional correlation spectroscopy analysis (FTIR-2D CoS), contact angle and zeta potential measurements, atomic force microscopy (AFM) and extended-Derjaguin-Landau-Verwey-Overbeek (ExDLVO) analysis. Our results show that high c-di-GMP levels promoted the secretion of long-chain hydrophobic and electroneutral extracellular polysaccharides and proteins. The protein molecules on WDL7/pYedQ2 promoted the bacterial self-aggregation and adhesion onto negatively charged surfaces. In contrast, the reduction of intracellular c-di-GMP concentrations resulted in a nearly 80 % decrease in the adhesion of bacterial cells, although little change in the surface hydrophobicity or surface charge properties were observed for these cells relative to the wild type. These results indicate that the reduced adsorption of WDL7/YhjH that we observed may be caused by the flagellum-accelerated mobility at low c-di-GMP concentrations. Taken together, these results improve our mechanistic understanding of the effects of c-di-GMP in controlling bacterial physical-chemical properties and initial biofilm development.
研究领域:
微生物学
·
分子生物学
·
生物物理化学
✓ 已验证
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2020
·
IF 3.784
Yangmin Wu,Wenting Wu,Wenjie Zhao,Xijian Lan
Although extensive experiments confirm the ability of copper for biofouling protection, its further utilization is still limited in virtue of unclear relationship between the surface topography and antibacterial performance. In this study, the hydrophobic copper surfaces with different morphologies, including nanowire, nanowire/microflower, and nanosheet/microflower hierarchical structures, were prepared through the one-step chemically etched method and in-situ self-assembly strategy. According to the four-time cyclic antibacterial assays for E. coli and B. subtilis , the copper with nanowire structure displayed a better antibacterial performance than others. Bacteriostasis rate of nanowire structure copper was over 90% in the first cycle, and reached 100% in the final test. In addition, the morphologies of copper surfaces after antibacterial test suggested that nanowire and microflower structures could significantly suppress the adhesion of bacteria. Besides, nanowire structure exerted an outstanding function in inhibiting bacterial adhesion under a lower concentration of inorganic salt.
研究领域:
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Liang Chaoan,Jiang Qiming,Yu Yi,Xu Tao,Sun Hanyu,Deng Feilong,Yu Xiaolin
Lithium (Li) reportedly has anti-bacterial properties. Thus, it is an ideal option to modify barrier membranes used for guided bone regeneration to inhibit the bacterial adhesion. The aims of this study were to fabricate and characterize nanofibrous poly(L-lactic acid) (PLLA) membranes containing Li, and investigate their antibacterial effects on Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in vitro. Li (5%Li, 10%Li, and 15%Li)-loaded nanofibrous PLLA membranes were fabricated using an electrospinning technique, and characterized via scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, a contact angle measuring device, and a universal testing machine. Sustained release of Li ions was measured over a 14-day period and biocompatibility of the Li-PLLA membranes was investigated. Evaluation of bacterial adhesion and antibacterial activity were conducted by bacterial colony counting, LIVE/DEAD staining and inhibition zone method using P.gingivalis and A.actinomycetemcomitans. Of the three Li-loaded membranes assessed, the 10%Li-PLLA membrane had the best mechanical properties and biocompatibility. Adhesion of both P.gingivalis and A.actinomycetemcomitans on Li-PLLA membranes was significantly lower than adhesion on pure PLLA membranes, particularly with regard to the 10%Li and 15%Li membranes. Significant antibacterial activity of Li-PLLA were also observed against according to the inhibition zone test. Given their better mechanical properties, biocompatibility, and antibacterial activity, PLLAs with 10%Li are a better choice for future clinical utilization. The pronounced antibacterial effects of Li-loaded PLLA membranes sets the stage for further application in guided bone regeneration.
研究领域:
生物材料学
·
口腔医学
✓ 已验证
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Hongyi Li,Yifu Wang,Xinxin Zhao,Zhen Yan,Chao Song,Shuguang Wang
Biofilm is the main cause of membrane biofouling and microbial corrosion . One of the most efficient strategies for biofilm reduction is inhibiting bacteria adhesion. In this study, we selected D -tyrosine to inhibit the adhesion of Escherichia coli , and the molecular response and metabolic pathways of E. coli were explored with transcriptome analysis . D -tyrosine could obviously inhibit the bacterial adhesion via lowering the adhesion force. The number of extracellular proteins decreased by 45% in the presence of D -tyrosine, leading to less hydrophobicity and autonomous aggregation of cells. Furthermore, transcriptome analysis showed that the inhibitory ability of D -tyrosine to the adhesion of E. coli decreased with time. At the initial stage, D -tyrosine could regulate tryptophan , curli, peptidoglycan , and adhesion-like protein synthesis , leading to less extracellular protein and lower cell hydrophobicity, and thus reduce cell aggregation and surface adhesion. This study provides a better understanding for the roles of D -amino acids in bacterial adhesion and develops a new strategy for biofilm reduction to mitigate membrane biofouling and microbial corrosion.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xiuhua Sun,Zhiren Guo,Qingmei Huang,Changlu Gao
The capsaicin analogue N-(4-hydroxy-3-methoxybenzyl) acrylamide (HMBA) was linked with polylauryl methacrylate-b-poly(2-(N,N-dimethylamino)ethyl methacrylate) (PLMA-b-PDMAEMA) via a quaternization reaction with 4-(acrylamidomethyl)-2-methoxyphenyl 2-chloroacetate (AAMPCA). The amphiphilic copolymers were capable of transforming its structure in response to the solvent change from aprotic to protic, which was verified by the 1H NMR spectrum. The resulting cationic copolymers underwent a hydrolysis process in water, yielding zwitterionic groups on surfaces. Meanwhile, the bactericidal reagent HMBA was released. It was proved that the hydrolysis rate of the copolymers accelerated with higher temperature, higher pH value, and higher hydrophilic block units. And the controllable, sustainable release of HMBA was achieved with copolymer-mediated hydrolysis. Protein-repellent and bactericidal tests on the surface of the coating proved that antifouling and bactericidal performances of the coating correlated to the structure conversion abilities of the corresponding copolymer. The dynamic monitoring of Escherichia coli adhesion in 3 h evidenced the antifouling and bactericidal process of copolymers with different block ratios and concentrations. The coating incorporated with 3% PLMA120-b-(PDMAEMA-AAMPCA)120 in polylactic acid base materials showed an adhesion ratio of E. coli less than 1% within 1 h, and the survival ratio of the adhered bacteria is <1%, suggesting its rapid speed and high efficiency in “bacterial repelling and killing”. Also, the PLMA120-b-(PDMAEMA-AAMPCA)120 copolymer demonstrated enhanced bactericidal ability compared with the mixture of cationic poly(laruyl methacrylate)120-b-poly(carboxybetaine methacrylate ester)120 (PLMA120-b-PCBMAE120) and free HMBA. The lowest minimal inhibitory concentration was 0.078 mg/mL against Staphylococcus aureus and 0.312 mg/mL against E. coli, respectively.
研究领域:
材料科学
·
微生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 97
·54372-54381
·
IF 3.708
Liujun Cheng,Qiongqiong Liu,Yufeng Lei,Yaling Lin,Anqiang Zhang
Nonspecific protein adsorption and bacterial adhesion have caused serious problems in biomedical devices, marine engineering, membrane separation and many other areas. In order to develop a water-soluble bio-adhesion resistant material, a series of novel zwitterionic polymers, carboxybetaine functionalized polysiloxanes (PDMS-g-CB), were synthesized via a three-step procedure. FT-IR and NMR were used to characterize the chemical structures of PDMS, and the solution properties (i.e. CMC) and biological characteristics were investigated. The high biocompatibility of PDMS-g-CB was demonstrated by hemolysis assay, skin irritation evaluation and acute oral toxicity testing. To evaluate the anti-fouling and hydrophilic properties of PDMS-g-CB, it was blended with PDMS elastomer to form films (b-PDMS). Water contact angle and ATR-FTIR measurements revealed that the hydrophobic PDMS surfaces were converted to hydrophilic surfaces after the introduction of PDMS-g-CB. The anti-fouling properties of b-PDMS were evaluated by protein adsorption and bacterial adhesion tests. Results showed that the amount of adsorbed protein and bacteria adhesion were significantly reduced compared with the untreated PDMS. These findings suggest that PDMS-g-CB is a biocompatible and promising material for the construction of anti-fouling surfaces.
研究领域:
生物材料学
·
高分子化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 76
·61742-61751
·
IF 3.84
Li Qun Xu,Ning Ning Li,Jiu Cun Chen,Guo Dong Fu,En-Tang Kang
Polymeric quaternary ammonium salts (QAS) have been widely studied for their antimicrobial activities. However, most of them were evaluated and used in solution form, which may limit their other applications, in medical and wound care and surface biofouling prevention. The development of antibacterial agents for use both in solution and in surface biofouling prevention are highly interesting and desirable. In this work, quaternized poly(2-(dimethylamino)ethyl methacrylate)-grafted agarose (Agr-g-QPDMAEMA) copolymers were synthesized via a combination of atom transfer radical polymerization (ATRP) and quaternization of tertiary amine moieties. The resulting Agr-g-QPDMAEMA copolymers can be dissolved in aqueous media at low concentrations to evaluate their antibacterial activity in the solution form. They can also be gelated as hydrogels on substrate surfaces to inhibit bacterial adhesion, biofilm formation and bacterial colonization. The Agr-g-QPDMAEMA copolymer hydrogels can be further fabricated into antibacterial patches to inhibit the bacteria growth on the contaminated surfaces.
研究领域:
材料科学
·
微生物学
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Ling Li Guo,Yan Fang Cheng,Xu Ren,Kasi Gopinath,Zhi Song Lu,Chang Ming Li,Li Qun Xu
Titanium (Ti) and its alloys are primarily explored to produce biomedical implants owing to their improved mechanical stability, corrosion resistance, low density, and good biocompatibility. Despite, Ti substrate surfaces are easily contaminated by plasma proteins and bacteria. Herein, a simple one-step process for the simultaneous deposition of a polyphenol tannic acid (TA) and four-armed poly(ethylene glycol) (PEG 10k -4−OH) on the Ti substrate (Ti-TA/PEG) surface was described. Additionally, a two-step process has been employed to fabricate the Ti-TA-PEG surface via successive deposition of TA and PEG 10k -4−OH for comparison. The resultant Ti-TA/PEG surface prepared by simultaneous deposition of TA and PEG 10k -4−OH exhibits higher coating thickness and better surface coverage than the Ti-TA-PEG surface. The Ti-TA/PEG and Ti-TA-PEG surfaces could actively inhibit the non-specific adsorption of proteins, suppress the bacterial and platelet adhesion, and prevents biofilm formation. Moreover, the Ti-TA/PEG surface displays a better antifouling performance than the Ti-TA-PEG surface. Thus, the present study demonstrates a simple and convenient approach for constructing polymeric coating with good anti-adhesive properties on the Ti substrate surface.
研究领域:
生物医学工程
·
材料科学
·
表面化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）