Biology-Basel
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2020
·Vol. 9
·期 7
·171
·
IF 3.796
Jianming Zhang,Yushan Bu,Chengcheng Zhang,Huaxi Yi,Daqun Liu,Jingkai Jiao
At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/106AU) than in the MRS broth (46.82 RMB/106AU).Keywords:bacteriocin Lac-B23;low-cost;high-efficiency;culture medium
研究领域:
微生物学
·
食品科学
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DOI
PubMed
高影响因子
Food & Function
·
2021
·Vol. 12
·期 4
·1695-1707
·
IF 5.396
Shumao Cui,Jie Jiang,Bowen Li,R. Paul Ross,Catherine Stanton,Jianxin Zhao,Hao Zhang,Bo Yang,Wei Chen
The role of Pediococcus pentosaceus in the gastrointestinne has received considerable attention in recent decades. This study aimed to investigate the effects of the short-term administration of P. pentosaceus on physiological characteristics, inflammation, and intestinal microecology in mice. In this study, 90 male C57BL/6J mice were divided into 15 groups, with 14 groups treated with a daily intragastric administration of different genotypes of P. pentosaceus. After three weeks of intragastric administration P. pentosaceus had a mild effect on mice. It could be seen that different P. pentosaceus strains had different effects on the gut microbiota and intestinal microecology. P. pentosaceus VCQYC5144M12 possessing an Enterolysin A operon may have been harmful, activating the expression of inflammatory factors, while P. pentosaceus DYNDL69M8 consisting of only a pediocin-like operon increased the abundance of beneficial bacteria and increased the content of acetic acid. The presence of various genotypes of bacteriocin may have been the explanation for variations among strains. This may provide theoretical support for further exploring the probiotic effect and patterns of P. pentosaceus.
研究领域:
微生物学
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免疫学
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营养学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
PROTEIN EXPRESSION AND PURIFICATION
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2015
·
IF 1.695
Fanqiang Meng,Haizhen Zhao,Chong Zhang,Fengxia Lu,Xiaomei Bie,Zhaoxin Lu
A potential bacteriocin gene was isolated from 18575 ORFs by bioinformatics methods. It was named pln1 , and cloned into pET32a. Then, it was expressed as a thioredoxin-Pln1 fusion protein in Escherichia coli BL21 (DE3). The fusion protein was purified by Ni-NTA, and thioredoxin was removed by enterokinase. Finally, Pln1 was purified using a cation affinity column. The yields of fused and cleaved Pln1 peptides were 100–110 mg/l and 9–11 mg/l, respectively. Pln1 was stable in an acidic environment and at temperatures below 60 °C, but was easily degraded under alkaline conditions and by protease treatment. The cleaved and purified Pln1 showed strong antimicrobial activity against gram-positive bacteria such as Micrococcus luteus CMCC 63202, Staphylococcus epidermidis , Lactococcus lactis NZ3900, Lactobacillus paracasei CICC 20241, and Listeria innocua CICC 10417. In particular, Pln1 had a better activity against methicillin-resistant S. epidermidis (MRSE) than nisin, thereby offering an attractive approach to counter bacterial antibiotic resistance .
研究领域:
微生物学
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分子生物学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
·
IF 7.183
Jinye Zhang,Sihang Li,Wenjing Wang,Jianfei Pei,Jiawei Zhang,Tianli Yue,Wirote Youravong,Zhenyu Li
Alicyclobacillus acidoterrestris is a thermophilic and acidophilic bacilli species which causes a significant decay in quality of apple juice and it cannot be efficiently treated by conventional sterilization procedures in food processing. In order to efficiently remove and inactivate Alicyclobacillus acidoterrestris in apple juice, a food functional microfiltration membrane was fabricated by a multi-stage modification including polydopamine coating and nisin grafting. Attenuated total reflectance-fourier transform infrared spectrometer and scanning electron microscopy images confirmed the success of immobilizing polydopamine and nisin microspheres onto the polyvinylidene difluoride membrane surface. In comparison with pristine polyvinylidene difluoride membrane, the modified membrane showed promoted performance in terms of hydrophilicity, water flux and the rejection of Alicyclobacillus acidoterrestris . A simultaneous exclusion and inactivation of Alicyclobacillus acidoterrestris in apple juice was achieved by modified microfiltration membrane. The modified membrane also exhibited reliable stability under different membrane cleaning procedures. The present study provides an option for the development of tailored food functional membrane for specific applications in food processing.
研究领域:
食品科学
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微生物学
·
膜技术
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Ke Ma,Wei Chen,Shi-Qing Yan,Zhen-Zhen Liu,Xiao-Qi Lin,Jia-Bao Zhang,Yu Gao,Tao Wang,Jian-Gang Zhang,Yong-Jun Yang
Antimicrobial compounds from the commensal gut microbiota have gained much attention due to their multifunctionality in maintaining good health in the host and killing multidrug-resistant bacteria. Our previous study showed that Paenibacillus jilinensis YPG26 isolated from chicken intestine can antagonize multiple pathogens. Herein, we characterized a bacteriocin-like inhibitory substance, jileicin, purified from P. jilinensis YPG26. Mass spectrometry analysis revealed that jileicin was a protein consisting of 211 amino acids, which showed 88.98% identity to the SIMPL domain-containing protein. The jileicin showed a relatively broad-spectrum antibacterial ability, especially against enterococci. Additionally, the jileicin exhibited good stability after various treatments, no detectable resistance, no significant cytotoxicity, and very low levels of hemolytic activity. The mode of action against Enterococcus faecium demonstrated that jileicin could destroy cell membrane integrity, increase cell membrane permeability, and eventually lead to cell death. Furthermore, jileicin was efficient in controlling the growth of E. faecium in milk. In conclusion, jileicin, as a newly identified antibacterial agent, is expected to be a promising candidate for application in the food, pharmaceutical, and biomedical industries.
研究领域:
微生物学
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食品科学
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Xinran Lv,Huanhuan Ma,Mengtong Sun,Yang Lin,Fengling Bai,Jianrong Li,Bolin Zhang
A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish ( Trichiurus lepturus ) and identified as Lactobacillus plantarum . This bacteriocin reached the maximum production at stationary phase after 24 h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30 min at 121 °C) and kept active at the pH range of 2.5–5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens , P. aeruginosa , Vibrio parahaemolyticus , Aeromonas sobria and Listeria monocytogenes . The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4 °C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative.
研究领域:
微生物学
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食品科学
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海洋生物学
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产品关联
DOI
高影响因子
Processes
·
2020
·Vol. 8
·期 3
·259
·
IF 2.753
Jing Zhang,Muhammad Bilal,Shuai Liu,Jiaheng Zhang,Hedong Lu,Hongzhen Luo,Chuping Luo,Hao Shi,Hafiz M. N. Iqbal,Yuping Zhao
Herein, a bactericide-secretingBacillusstrain, potentially useful as a biocontrol agent, was isolated from the commercial Yanjing Natto food. Following the biochemical and physiological evaluation, the molecular identification was performed using 16S rDNA sequencing of polymerase chain reaction-amplified DNA that confirmed the natto isolate asBacillus subtilisnatto (B. subtilisnatto). The biocontrol (microbial inhibitory) capability ofB. subtilisnatto was investigated againstStaphylococcus aureus,Escherichia coli,Salmonella typhimurium, and yeast (Yarrowia lipolytica) and recorded. The antimicrobial activity ofB. subtilisnatto was further enhanced by optimizing the growth medium for optimal bactericides secretion. Under optimized conditions,B. subtilisnatto exhibited much higher inhibitory activity againstS. aureuswith a zone of inhibition diameter up to 27 mm. After 48 h incubation, the optimally yieldedB. subtilisnatto broth was used to extract and purify the responsible bactericides by silica gel column chromatography, gel column chromatography, and semi-preparative high-performance liquid chromatography. Structural identification of purified bactericides (designated as NT-5, NT-6, and NT-7) fromB. subtilisnatto was performed by13C-nuclear magnetic resonance (NMR) and mass spectral analyses. The NMR comparison also revealed that NT-5, NT-6, and NT-7 had identical structures, except for the fatty chain. In summary, the present study suggests the improved biocontrol and/or microbial inhibitory potential of newly isolated bactericides secretingB. subtilisnatto.Keywords:biocontrol agent;Bacillus subtilisnatto;isolation;molecular identification;medium optimization;antimicrobial activity;bactericides;spectral analyses
研究领域:
微生物学
·
生物技术
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Cell Genomics
·
2025
·
IF 9
Dengwei Zhang,Yinai Zou,Yuqi Shi,Junliang Zhang,Jing Liu,Gengfan Wu,Jian Zhang,Ying Gao,Muxuan Chen,Yong-Xin Li
Human gut microbiota produces unmodified bacteriocins, natural antimicrobial peptides that protect against pathogens and regulate host physiology. However, current bioinformatic tools limit the comprehensive investigation of bacteriocins’ biosynthesis, obstructing research into their biological functions. Here, we introduce IIBacFinder, a superior analysis pipeline for identifying unmodified class II bacteriocins. Through large-scale bioinformatic analysis and experimental validation, we demonstrate their widespread distribution across the bacterial kingdom, with most being habitat specific. Analyzing over 280,000 bacterial genomes, we reveal the diverse potential of human gut bacteria to produce these bacteriocins. Guided by meta-omics analysis, we synthesized 26 hypothetical bacteriocins from gut commensal species, with 16 showing antibacterial activities. Further ex vivo tests show minimal impact of narrow-spectrum bacteriocins on human fecal microbiota. Our study highlights the huge biosynthetic potential of unmodified bacteriocins in the human gut, paving the way for understanding their biological functions and health implications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2021
·
IF 3.066
Zhijing LIU,Cong XU,Ran TIAN,Wan WANG,Jiage MA,Liya GU,Fei LIU,Zhanmei JIANG,Juncai HOU
Lactic acid bacteria (LAB) are a representative probiotic. As the dominant flora in the human intestinal tract, LAB can regulate the balance of human intestinal flora and improve host health. The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population, with excellent probiotics and high antibacterial activity. After 16S ribosomal RNA (rRNA) homology and phylogenetic tree analysis, potential probiotics were tested for their antibacterial activity, resistance to artificial gastrointestinal fluid and drugs, surface hydrophobicity, and safety. Three strains of LAB with acid resistance, bile salt resistance, epithelial cell adhesion, and no multidrug resistance were selected: Lactobacillus salivarius , Leuconostoc lactis , and Lactobacillus paracasei . Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L. lactis were organic acids, whereas those of L. salivarius and L. paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity. These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.
研究领域:
微生物学
·
食品科学
·
医学微生物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2020
·
IF 3.067
Hong Yan,Yingying Lu,Xin Li,Yanglei Yi,Xin Wang,Yuanyuan Shan,Bianfang Liu,Yuan Zhou,Xin Lü
Bacteriocins have potential in the food industry, especially in protecting food safety and controlling food-borne diseases. Class II bacteriocin genes were previously identified from the genome of Lactobacillus crustorum MN047 and successfully expressed in Escherichia coli BL21 (DE3) using the pET-30a system. In this study, the antibacterial mechanism and potential use as a food preservative were studied. The results showed that bacteriocin BM1829 had antimicrobial activity against Gram-positive and Gram-negative bacteria with MIC values of 1–16 mg/L. In addition, BM1829 showed good stabilities to chemicals, and to thermal and pH changes. BM1829 destroyed the integrity of membranes and led to the leakage of cytoplasmic content. Flow cytometry showed that BM1829 arrested the cell cycle at R-phase. Moreover, the number of E. coli and Staphylococcus aureus cells was reduced during beef storage with BM1829 treatment. These data suggested that BM1829 has the potential to be developed as a food preservative or antibacterial agent.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 19
·3377
·
IF 5.719
Wenqian Huang,Yirong Zhang,Zhi Li,Minjie Li,Fangfang Li,Monika Mortimer,Liang-Hong Guo
Medical applications of nanotechnology are promising in creating efficient and targeted therapies. However, so far, nanodrug design has not taken into consideration possible effects on human microbiota. The beneficial functions of bacteria could be stimulated by nanodrugs while negative effects on beneficial bacteria could cause risks to human health. Here, simulated intestinal fluid (IF) was optimized for culturing a human commensal and probiotic bacterial strain,Lactobacillus casei, to study the effects of medically relevant NPs—Ag and hyaluronic acid-coated Au NPs (HA-Au NPs)—in conditions pertinent to the gastrointestinal tract. When cultivated either aerobically or anaerobically, the specific growth rates ofL. caseiwere ~0.2 h−1in IF and ~0.4 h−1in the standard medium of lactobacilli (MRS). Ag NPs inhibited the growth ofL. caseiin IF at lower concentrations (EC50~ 65 and 15 mg/L in aerobic and anaerobic conditions, respectively) than in MRS (EC50> 100 mg/L), likely caused by differences in the composition of the two media and different intrinsic growth rates of bacteria in IF and MRS. Ag NP dissolution in IF and MRS did not explain the differences in growth inhibition, implying NP-specific effects. HA-Au NPs were not growth-inhibitory toL. caseiup to 250 mg/L. Still, both NPs at sub-growth-inhibitory concentrations suppressed the expression of bacteriocin genes inL. casei, suggesting an inhibitory effect of NPs on the probiotic properties ofL. casei, i.e., its competitiveness in microbial communities. However, HA-Au NPs did not appear to affect or even stimulated the immunomodulatory properties ofL. caseiin human intestinal epithelial cells. Thus, medically relevant NPs at low, sub-bacteriostatic levels can affect the metabolism of beneficial human bacteria and potentially induce changes in the microbiota and immune signaling.Keywords:commensal bacteria;microbiota;bacteriocin;Caco-2;intestinal fluid;antibacterial
研究领域:
纳米技术
·
微生物学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Qibin Li,Quanyi Chen,Yueying Wu,Zhibao Chen,Ying Liu,Zhijia Fang,Qi Deng
The bacteriocin CAMT6 is a novel bacteriocin produced by Enterococcus durans YQ-6 isolated from Larimichthys polyactis in the South China Sea. In this study, the bacteriocin CAMT6 was purified by extraction using ethyl acetate, Sephadex LH-20 column chromatography, cation exchange chromatography and reversed-phase high-performance liquid chromatography, in sequence. The Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS) analysis revealed that the relative molecular mass and amino acid sequence of the bacteriocin CAMT6 were 1254.63 Da and N-GAVHDVKDVLDS, respectively. Combined with the circular dichroism (CD) spectroscopy results, CAMT6 was determined to be a novel Class IId bacteriocin. CAMT6 exhibited broad-spectrum antibacterial activity, inhibiting both gram-negative and gram-positive bacteria, even fungi. CAMT6 exhibited strong temperature stability, was stable over pH 2–7, and was inactivated by proteases. CAMT6 also significantly removed L. monocytogenes biofilms and inhibited L. monocytogenes by disrupting the permeability of the cell membrane. Furthermore, CAMT6 can effectively inhibit the growth of L. monocytogenes in chicken breast. These results suggest that the bacteriocin CAMT6 could serve as a potential biological preservative for food.
研究领域:
微生物学
·
食品科学
·
生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）