MOLECULES
·
2023
·Vol. 28
·期 2
·596
·
IF 4.6
Qiongqiong Wang,Shiqi Liu,Long Xu,Bin Du,Lijun Song
Polysaccharides are the main bioactive components in safflower. In this study, safflower polysaccharides (SPs) were extracted by ultrasonic assisted extraction, and four purified safflower polysaccharide fractions (named SSP1, SSP2, SSP3, and SSP4, respectively) were obtained. The physicochemical properties and in vitro physiological activities of the four fractions were investigated. The molecular weights (MW) of the SSPs were 38.03 kDa, 43.17 kDa, 54.49 kDa, and 76.92 kDa, respectively. Glucuronic acid, galactose acid, glucose, galactose, and arabinose were the main monosaccharides. The Fourier transform infrared spectroscopy (FT-IR) indicated that the polysaccharides hadα- andβ-glycosidic bonds. Nuclear magnetic resonance (NMR) analysis showed that SSP1 had 6 different types of glycosidic bonds, while SSP3 had 8 different types. SSP3 exhibited relatively higher ABTS+scavenging activity, Fe+3-reduction activity, and antiproliferative activity. The results will offer a theoretical framework for the use of SPs in the industry of functional foods and medications.Keywords:safflower;polysaccharides;structures characterization;physiological activities
研究领域:
植物化学
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功能食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Yu-Xiao Wang,Ting Zhang,Yue Xin,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Agrocybe cylindracea is a common source of active polysaccharides, but their fine structures are not clearly elucidated. In the present study, four fractions were purified from the alkaline extract of A. cylindracea (JACP), and their chemical components and structures were compared by HPAEC-PAD, methylation combined with GC–MS, and 1D/2D NMR analysis. Results showed the purified fractions’ physicochemical properties, including monosaccharide compositions, molecular weights, viscosities and surface morphology considerably varied. JACP-30 was identified as a fucoglucogalactan with a α-(1 → 6)-galactopyranosyl as main chain. JACP-50p and JACP-80r were characterized as β-(1 → 6)-glucans with side chains composed of terminal and 3-substituted β-glucopyranosyl residues attached at O -3 for every three residues. Similarly, the backbone of JACP-80 was β-(1 → 6)-linked glucopyranosyl and β-(1 → 3,6)-linked glucopyranosyl residues at a ratio of 4:1. This work provides more information to the understanding of polysaccharides from A. cylindracea , further guiding its biological researches and developing the application in food and biomedicine industries.
研究领域:
食品科学
·
生物化学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Lishan Xu,Yaojie Zhang,Lizhi Wang
Dragon fruit is a tropical fruit with good taste. It can bring health benefits to human body. As one of the major bioactive components in this fruit, the polysaccharides might contribute to the health benefits. However, the precise structure information remains unknown. A leading polysaccharide of dragon fruit pulp, DFPP, was purified and identified by NMR and GC–MS. →4-β- d -Glc p A-1→, →6-β- d -Gal p -1→ and →4-α- l -Rha p -1→ constituted the backbone and α- l -Ara f -1 → 5-α- l -Ara f -1→ formed the branch chain. The precise structure was putatively identified as below. The molecular weight was 2.2 × 10 3 kDa. The structure information of polysaccharides will be helpful to understand this fruit. Download : Download high-res image (88KB) Download : Download full-size image
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 9
·3636-3643
·
IF 3.639
Jianyuan Jing,Shuiqin Zhang,Liang Yuan,Yanting Li,Yingqiang Zhang,Yanchen Wen,Bingqiang Zhao
BACKGROUND Humic acid (HA)-enhanced urea (HAU) is the top-selling efficiency-enhanced urea in China. Comprehensive investigation into the structure and efficacy of HA complex formation with urea (HACU) – the main reaction product during HAU's production – is required to clarify the reaction mechanism between HA and urea, and to provide guidance for the development of high-efficiency HAU. RESULTS HACU showed discrepant structural and compositional features from raw HA. Nitrogen (N) content in HACU was 7.3 times greater than that of HA. Several high-resolution analytical methods showed a sharp increase of ammonia in the gaseous product during HACU pyrolysis, suggesting that urea contributed N to HACU. HACU was characterized with significantly fewer carboxyl groups than in raw HA, implying that the carboxyl group was the main group in HA to participate in the reaction between HA and urea. The presence of amide-N in HACU verified the structure of the reaction product. Furthermore, both HACU and HA could enhance the biomass in hydroponically grown maize seedlings, but the highest stimulation for HACU came about when its carbon concentrations were 50–100 mg L −1 , higher than the optimal carbon concentration for HA (25 mg L −1 ), attributed to the lower carboxyl group content for HACU to some extent. CONCLUSION During HAU's production, reaction with N derived from urea to form amide-N decreased the carboxyl groups in HA, leading to higher concentrations for HACU required to achieve the similar bioefficacy of HA. © 2021 Society of Chemical Industry.
研究领域:
农业科学
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植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yu-Xiao Wang,Ting Zhang,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Polysaccharide from Agrocybe cylindracea has been demonstrated to exhibit excellently biological functions, but insufficient understanding of its physicochemical property and fine structure limits its wide application and development. In the present study, two water-soluble heteroglycans, a high molecular weight (SACP-40) and a low molecular weight (SACP-80), were isolated from the fruit bodies of A. cylindracea by hot water extraction and gradient ethanol precipitation. There was little difference in their physicochemical properties, including major chemicals and monosaccharide compositions. Rheological measurements revealed SACP-80 was a typical pseudoplastic fluid with shear thinning behavior at the concentration of 3.0%, whereas exhibited a near-Newtonian fluid at lower concentration. SEM observation suggested SACP-80 showed micro-spherical shapes loosely attached to fibrous filament in its surface. Besides, methylation and 1D/2D NMR analysis indicated that SACP-80 was a branched heteroglycan with a backbone composed of β-(1 → 6)-glucopyranosyl, α-(1 → 6)-galactopyranosyl and α-(1 → 2,6)-glucopyranosyl residue at a ratio of 3:1:1. This work provides a theoretical basis for gaining insight into the relationship between the fine structure and viscosity of polysaccharide from A. cylindracea , which could further guide its functional studies and advance the potential application in food and biomedicine industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Xuejing Jia,Chao Zhang,Jianfeng Qiu,Lili Wang,Jiaolin Bao,Kai Wang,Yulin Zhang,Meiwan Chen,Jianbo Wan,Huanxing Su,Jianping Han,Chengwei He
Three novel acidic polysaccharides termed PRM1, PRM3 and PRM5 were purified from Rhynchosia minima root using DEAE-52 cellulose and sephadex G-150 column chromatography. Their structures were characterized by ultraviolet (UV) and Fourier transform infrared (FTIR) spectrometry, gel permeation chromatography (GPC), gas chromatography–mass spectrometry (GC–MS), and differential scanning colorimeter (DSC) analysis. The uronic acid contents of PRM1, PRM3 and PRM5 were 30.7%, 12.7% and 47.7%, respectively. PRM1 (143.2 kDa), PRM3 (105.3 kDa) and PRM5 (162.1 kDa) were heteropolysaccharides because they were composed of arabinose, mannose, glucose and galactose. Their enthalpy values were 201.0, 111.0 and 206.8 J/g, respectively. PRM3 and PRM1 exhibited strong in vitro anticancer activity against lung cancer A549 and liver cancer HepG2 cells in a dose-dependent manner. These findings suggested that PRM1 and PRM3 could be potentially developed as natural anticancer agents.
研究领域:
植物化学
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药理学
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肿瘤学
✓ 已验证
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yu-Xiao Wang,Ting Zhang,Jun-Yi Yin,Xiao-Jun Huang,Jun-Qiao Wang,Jie-Lun Hu,Shao-Ping Nie
A homogeneous β-glucan (JHMP-70) was obtained from Hypsizygus marmoreus by alkali extraction and ethanol precipitation, and had an apparent molecular weight of 394 kDa. Its fine structure was investigated by FT-IR, methylation combined with GC-MS and 1D and 2D NMR analysis. Results indicated JHMP-70 was a branched β-(1 → 6)-glucan substituted at O -3 by the non-reducing β-Glc p and/or β-(1 → 3)-linked β-Glc p side chains at a ratio of 1:1. SEM observation revealed that there were micro-spherical shapes intertwined with randomly distributed fibrous filaments. Rheological measurements showed that its flow behavior was highly dependent on the concentration. Newtonian behavior was evident at low concentration, whereas pseudoplastic behavior was observed at high concentration. Besides, the addition of salt ions (Na + or Ca 2+ ) significantly decreased the apparent viscosity of JHMP-70 solution. The low viscosity of JHMP-70 was probably attributed to its structural features, including main chain structure, branching degree and side chain length. This work can provide a basis theory to expand the application of the polysaccharides from H. marmoreus in food industry, because their lower viscosity and superior fluidity are desired in beverage products to impart stability against phase separation without compromising other sensorial properties.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Shihai Zhang,Chunqian Zhang,Meixia Li,Xia Chen,Kan Ding
Crataegus pinnatifida is widely used as functional food and traditional medicine in China for various applications. Polysaccharides may contribute to health benefits, such as modulating human gut microbiota (HGM). To investigate structure features of polysaccharides from this plant, fruits of C. pinnatifida were extracted with boiling water, yielding crude polysaccharides HAW. A novel polysaccharide, named HAW1-1, was pooled by DEAE Sepharose™ Fast Flow column and further purified by Sephacryl S-100 HR column. The molecular weight of HAW1-1 was 42.35 kDa. The carbohydrate content of HAW1-1 was 99.42% and no protein was detected. The monosaccharide analysis showed this polysaccharide was only composed of glucose. According to the partial acid hydrolysis, methylation analysis and NMR spectra, the backbone of HAW1-1 was consisted of 1,4-linked α-D-glucan and 1,4,6-linked α-D-glucan. The side chains were composed of 1,6-linked α-D-glucan and terminal-linked α-D-glucan. The bioactivity tests showed that HAW1-1 could promote the growth of three species of intestinal Bacteroides and produce short-chain fatty acids (SCFAs). The results suggested that HAW1-1 might be a potential prebiotic candidate to maintain intestinal homeostasis and improve human gut health.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Wei Tang,An-Qi Li,Jun-Yi Yin,Shao-Ping Nie
Three water-soluble pectic polysaccharides (WKBP-P2, P3 and P4) were isolated from white kidney bean by ion exchange combined with size-exclusion methods. The structural features were characterized by GC–MS, NMR spectroscopy and HPSEC-MALLS-RI. It was found that three pectic polysaccharides were the major water-extracted polysaccharides in white kidney bean. All the WKBP-P2, P3 and P4 were probably composed of various structural regions including homogalacturonan (HG), xylogalacturonan (XGA), rhamnogalacturonan I (RG-I) regions in backbone, and arabinan region mainly as side chain. However, these pectic polysaccharides were significantly different in molar ratios of these structural regions and molecular size. WKBP-P2 was HG-predominant pectin (partially methyl-esterified) with weight-average molecular weight ( M w ) of 1.2 × 10 4 g/mol, and contained minor RG-I, arabinan and probable XGA regions. WKBP-P3 ( M w of 4.0 × 10 4 g/mol) primarily embraced XGA, HG, arabinan regions and minor RG-I region. WKBP-4 with highest M w (4.5 × 10 5 g/mol) had the most arabinan region (51.3%), which was probably the side chain linked to the backbone composed of RG-I, HG and slight XGA regions. These findings provided a structural basis for study on polysaccharides from white kidney bean, which was benefit for development of functional food.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Zezhou Lin,Tianyu Li,Qian Yu,Haiyun Chen,Di Zhou,Ning Li,Chunyan Yan
Achyranthes bidentata is a species of flowering plant that is mainly distributed in China. The A. bidentata rhizome is a famous traditional Chinese medicine that has been widely used to treat lumbago, arthritis, and bone hyperplasia. In this work, A. bidentata rhizome was isolated and purified to obtain a pectic polysaccharide (ABPB-4). Chemical and spectral analyses showed that ABPB-4 had a main chain of →4)- α - d -Gal p A-(1→ and →2,4)- α - l -Rha p -(1→, and the branch chains included →4)- β - d -Gal p -(1→, →6)- β - d -Gal p -(1→, →3,6)- β - d -Gal p -(1→, →5)- α - l -Ara f -(1→ and →3,5)- α - l -Ara f -(1→, and it was terminated with α - l -Ara f -(1→ and β - d -Gal p -(1→. At concentrations of 0.01, 0.02, and 0.04 μmol/L, ABPB-4 significantly promotes the proliferation, differentiation, and mineralization of MC3T3-E1 cells in vitro , and it appreciably enhances the mRNA expression levels of osteogenic-related genes in these cells. Overall, the results reported herein indicate that ABPB-4 has outstanding osteogenic activity, and that it may be used as an anti-osteoporosis agent in the future.
研究领域:
中药学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jin-Jin Liu,Shi-Kang Chen,Xin Wang,Wei-Wei He,Xiao-Xiao Song,Xiao-Jun Huang,Jun-Yi Yin,Shao-Ping Nie
Polysaccharides derived from Agrocybe cylindracea have been demonstrated to exhibit various bioactivities. However, studies on their structural characteristics during the growth process are limited. This study aimed to compare the physicochemical properties and structural characteristics of alkali-extracted polysaccharides from A. cylindracea fruiting bodies (JACP) across four growth stages. Results showed that the extraction yields and protein levels of JACP declined along with the growth of A. cylindracea, while the contents of neutral sugar and glucose increased significantly. However, JACP exhibited structural characteristics similar to those across the four stages. Four polysaccharide subfractions were isolated from each growth stage, including JACP-Et30, JACP-Et50, JACP-Et60, and JACP-Et70. JACP-Et30 from the four stages and JACP-Et50 from the initial three stages were identified as heteroglucans with β-1,3-d-Glcp and β-1,6-d-Glcp residues as main chains, respectively. However, other subfractions were considered as β-1,6-d-glucans containing minor glucuronic acid. These subfractions were predominantly replaced by Glcp residues at the O-3 and O-6 positions. Overall, while JACP exhibited variable physicochemical properties, its structural characteristics remained stable during the growth process, offering new insights into its potential applications in the food and medicinal industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Yan Chen,Xue-Hui Li,Li-Yuan Zhou,Wan Li,Liu Liu,Dan-Dan Wang,Wen-Na Zhang,Sajid Hussain,Xiao-He Tian,Yong-Ming Lu
Tricholoma lobayense is a nutritious mushroom with great health benefits. Three polysaccharides with purity higher than 99% were successfully extracted from Tricholoma lobayense . The molecular weights of TLH-1, TLH-2 and TLH-3 were determined to be 8.43 × 10 5 ，5.36 × 10 5 and 4.53 × 10 3 Da, respectively. The backbones of TLH-1 and TLH-2 were mainly composed of 1,4-linked α- d -glucopyranosyl. However, polysaccharide TLH-3 was found to be a highly branched glucogalactan, which is made up of 1,3-linked α- d -glucopyranosyl branched at C-6 and 1,3-linked β - d -galactopyranosyl. In vitro antioxidant activity assays revealed that TLH-3 exhibited highest antioxidant activities among the polysaccharides from Tricholoma lobayense , which were comparable to those of ascorbic acid. The results suggested that the outstanding antioxidant activities of TLH-3 might depend on its low molecular weight, high branch degree, versatile linkage types and complex conformation. These characteristics make TLH-3 an attractive natural antioxidant for food and pharmaceutical applications.
研究领域:
食品科学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）