ACS Nano
·
2025
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IF 16
Shuya Wang,Xiaojing Shi,Tingli Xiong,Wenxuan Zheng,Ruishi Li,Weimin Qi,Min Liu,Lin Dai,Min Zhou,Wei Dai,Qiong Huang,Xiaoying Wang,Kelong Ai
The strategy of restoring mitochondrial function by modulating mitochondrial membrane potential (MMP) through uncoupling protein 2 (UCP2) offers significant therapeutic potential against cerebral ischemia-reperfusion injury (CIRI). However, traditional strategies ignored elevation of mitochondrial reactive oxygen species (mtROS) resulting from UCP2 inhibition, resulting in poor therapeutic effects. Here, we reported a multifunctional SGB nanomedicine formed by pioneering the prepolymerization of the UCP2 inhibitor genipin and glycine and a cerebral infarction targeting peptide via a metastable imine bond. After intravenous injection, SGB was highly targeted to affected brain tissue and reached neuronal mitochondria. SGB could not only restore MMP by cleaving the metastable imine bond to release genipin to inhibit overexpressed UCP2, but also simultaneously eliminated excessive mtROS. Compared with traditional UCP2 inhibition, SGB could not only significantly improve the bioavailability of genipin and reduce systemic side effects, but also effectively protected neuronal mitochondria, reduced endoplasmic reticulum stress and inhibited the inflammatory storm of microglia, ultimately significantly reduced neuronal apoptosis. Correspondingly, SGB nearly reversed CIRI with a low 5 mg/kg dose. This innovative approach redefines the role of UCP2 inhibition and provides a framework for the treatment of CIRI by maintaining mitochondrial function.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Hongyan Lu,Ying Cheng,Xiangling Pu,Xingtao Zhao,Ruolan Li,Ruiqi Sun,Xiaoman Xie,Mingyue Wu,Shuo Zhang,Qiong Pan,Jin Chai
Simultaneously alleviating oxidative stress and remodeling inflammatory microenvironment holds great promise for treating inflammatory bowel disease (IBD). Nevertheless, the efficacy of conventional therapies remains limited by poor targeting, insufficient modulation of the inflammatory microenvironment and serious adverse effects. Here, inspired by the intrinsic biological properties of metal-curcumin (Cur) complexes, a nanozyme-mediated ion-exchange strategy was proposed to enhance the targeted therapeutic effect of Cur for IBD. Specifically, copper-doped mesoporous polydopamine nanoparticles (Cu-mPDA) with dual enzyme-like activities were served as drug carriers to encapsulate water-soluble zinc-curcumin (Zn-Cur) and further modified with hyaluronic acid (HA), yielding Zn-Cur/Cu-mPDA@HA. Encouragingly, Zn-Cur/Cu-mPDA@HA could degrade in inflammatory microenvironment, releasing Zn-Cur and Cu 2+ , which subsequently in situ generate Cu-Cur with high antioxidant activity by ion-exchange reaction. Notably, Zn-Cur/Cu-mPDA@HA not only exhibited favorable targeting specificity toward M1 macrophages in inflamed colon tissues, but also displayed enhanced abilities to scavenge ROS, inhibit pro-inflammatory factors secretion and promote macrophages repolarization from the pro-inflammatory M1 state to the anti-inflammatory M2 subtype. Mechanistically, Zn-Cur/Cu-mPDA@HA suppressed the TLR4/MyD88/NF-κB pathway, breaking the inflammation-free radical vicious cycle, thereby attenuating chronic inflammation and fostering a favorable microenvironment for intestinal barrier restoration. Such a nanozyme-mediated ion-exchange and in situ drug generation strategy offers a new insight for designing nanomedicines for the treatment of IBD and other inflammatory diseases.
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Small
·
2026
·
IF 11.8
Yitian Zhang,Dianji Li,Minhao Liu,Ziqing Tang,Hui Yu,Min You,Qiang Chen,Xi Ding
Highly wet, dynamic, inflammatory, and fouling environments were significant challenges in the healing of diabetic oral ulcers. Inspired by the oral mucosa, a wet-adhesion Janus hydrogel (WAJH) with asymmetric bifunctionalities of antifouling/antioxidant was proposed for diabetic oral ulcer treatment. In the WAJH, the antifouling layer was composed of the agar/polyacrylamide double-network (Agar/PAAm DN) hydrogel, and the wet-adhesive layer was constructed by the poly(acrylic acid-co-2-phenoxyethyl acrylate)/tannic acid (PAEA/TA) hydrogel. The antifouling layer mimicked the oral epithelium to prevent pollutant invasion, and the adhesive layer imitated the basement membrane of oral mucosa to adhere firmly to wet diabetic oral ulcers. Importantly, the adhesion energies of the two layers were distinctly different, measuring 15 J m −2 for the antifouling layer and 316 J m −2 for the adhesive layer, respectively. Notably, TA not only significantly enhanced the toughness and wet adhesion but also endowed the WAJH with reactive oxygen species (ROS) scavenging and antioxidant capabilities. The WAJH remarkably accelerated the healing of oral ulcer by inhibiting inflammation at the ulcer, promoting angiogenesis, and promoting re-epithelialization in vivo. Consequently, the WAJH with antifouling and antioxidant exhibited promising prospects in the clinical treatment of diabetic oral ulcer.
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Biomaterials Science
·
2026
·
IF 6.1
Zixin Zhang,Yuxin Wang,Bingjie Sun,Huaqiang Luo,Bingtian Tu,Huan He,Jing Tu
Chronic diabetic wounds are marked by recurrent infection, persistent ROS and prolonged inflammation. Herein, we report a MOF-reinforced NIR-responsive composite hydrogel (CEPN) that features a dual-cross-linked network assembled from carboxymethyl chitosan (CMCS), polydopamine (PDA), epigallocatechin gallate (EGCG) and L-Cys@Cu MOF nanofibers. The CEPN composite hydrogel is fabricated via a facile one-pot procedure and undergoes rapid in-situ gelation while exhibiting robust tissue adhesion at room temperature. The composite hydrogel integrates ondemand photothermal sterilization, broad-spectrum antioxidant capacity and enhanced mechanical robustness. Moreover, it efficiently scavenges reactive oxygen species (ROS) including O 2 •-, H 2 O 2 , •OH, and neutralizes the synthetic radicals DPPH• and ABTS •+ . Upon near-infrared (NIR) irradiation, the composite hydrogel rapidly generates localized hyperthermia, reaching 60 °C within 8 min, and exhibits excellent bactericidal efficacy against both Gram-negative Escherichia coli (E. coli) and Grampositive Staphylococcus aureus (S. aureus). In vitro and in vivo studies confirm that the composite hydrogel is highly biocompatible and also promotes cell migration and angiogenesis. In a diabetic mouse wound model, the composite hydrogel significantly reduces the wound area and accelerates tissue regeneration. Overall, this multifunctional composite hydrogel offers a simple, safe and effective treatment strategy for chronic diabetic wounds.
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Yizhe Ding,Jiacheng Shi,Yuqian Lin,Qiya Sun,Yuxiang Zhao,Xi Wang,Qian Ping,Lin Wang,Yongmei Li
In this study, a novel riboflavin-mediated ultraviolet/peracetic acid system (RF/UV/PAA) was developed to enhance the degradation of ranitidine. Under optimal conditions, ranitidine was completely removed within 15 min, leading to a 68.9% mineralization rate due to the combined action of radical (hydroxyl radicals (·OH) and carbon-centered organic radicals (R-C·) and nonradical (singlet oxygen ( 1 O 2 )) reactive oxidative species (ROS). Electron paramagnetic resonance and quenching experiments indicated that RF significantly promoted ROS generation, with the respective contributions of ·OH, R-C· and 1 O 2 to ranitidine removal being 54.5%, 15.8%, and 24.3%, respectively. Demethylation, monooxygenation, dioxygenation, and C-S bond cleavage were identified as the primary degradation pathways for ranitidine. These pathways were initially inferred from structural analysis of transformation products (TPs) and subsequently validated through density functional theory (DFT) calculations and the TP-Transformer model. Furthermore, an integrated DFT-machine learning (ML) framework was employed to elucidate the degradation mechanism, with a specific focus on the interactions of principal molecular features with ROS. The results revealed that FractionCSP3 (SHAP value = 0.30) and MolWt (SHAP value = 0.44) were the dominant predictors of ·OH and 1 O 2 reactivity, respectively, whereas FormalCharge (SHAP value = 0.40) governed the R-C·-mediated processes. In addition, SHAP-based interaction analysis revealed that electron-rich sites facilitate ·OH attack, conjugated π-systems promote 1 O 2 reactivity, and molecular polarization drives R-C· reactions. Our study provides not only an effective technology for ranitidine removal but also a quantitative, interpretable framework for predicting reactivity and guiding the design of photo-assisted advanced oxidation processes.
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JOURNAL OF POWER SOURCES
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2026
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IF 8.4
Jinyang Chen,Xiaoyu Lin,Wei Wang,Li Gan,Zuliang Chen
The microbial reduction of graphene oxide (GO) represents a sustainable approach for producing functional nanomaterials, yet the mechanisms in Gram-positive bacteria remain poorly understood. In this study, Lysinibacillus sphaericus HJ-3 was shown to effectively reduce GO through both direct cytochrome-mediated electron transfer and flavin-dependent shuttling. Whole-genome sequencing revealed complete respiratory chain and flavin biosynthesis pathways, while metabolomic profiling indicated enrichment of riboflavin and aromatic amino acid metabolism under GO stress. qRT-PCR analysis further confirmed the upregulation of key electron transport genes, particularly c-type cytochromes, during GO reduction. Control experiments, including abiotic, heat-killed, and non-reducing Gram-positive strains, verified that the process is a genuine biotic activity requiring metabolic viability. Together, these findings provide multi-omics evidence for the extracellular electron transfer mechanism of GO reduction in L. sphaericus and highlight its adaptive responses to redox stress. Importantly, the study demonstrates that endogenous flavins act as efficient electron shuttles to enhance electron transfer, thereby facilitating the microbial reduction of GO. This work not only deepens the understanding of extracellular electron transfer in Gram-positive bacteria but also underscores the potential of microbial strategies as green and scalable approaches for advanced graphene-based materials.
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ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Zhuoheng Jiang,Zhaoyang Yue,Shuncheng Yao,Wenting Chen,Chuyu Tang,Quanhong Hu,Songjing Zhong,Linlin Li
Osteoarthritis (OA) presents a formidable therapeutic challenge due to the relentless progression of cartilage degradation, the intricate management of both pain and inflammation, and the difficulty in halting disease advancement into later stages. Here, we present a noninvasive, self-powered electrotherapy system for OA treatment, integrating a wearable triboelectric nanogenerator (TENG) with electro-responsive ROS-scavenging nanozymes. The TENG converts body movement into electricity, providing electroacupuncture (EA)-mimetic stimulation through microneedle electrodes. This approach minimizes tissue damage risks and liberates patients from mobility constraints. Under the TENG-generated frictional electric field, the electro-responsive CuxMn3-xO4 nanozymes demonstrate enhanced catalytic activity to effectively scavenge ROS, reprogramming the immune microenvironment toward an anti-inflammatory state, ultimately achieving superior therapeutic efficacy in rat model of OA. This synergistic approach offers a promising, patient-friendly platform with the potential to redefine OA therapeutic strategies and a broad range of inflammatory diseases, including psoriasis and encephalitis.
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Advanced Healthcare Materials
·
2026
·
IF 9.6
Yinuo Yang,Jian Shen,Keyi Huang,Haimang Wang,Yiran Jia,Xin Zhang,Yang Jiao,Xiechuan Weng,Hongyu Zhang,Zhenhong Fu
Cardiovascular diseases, particularly acute myocardial infarction (AMI), have posed a significant global health burden due to the high morbidity and mortality rates. Current treatment methods are compromised by a narrow therapeutic window, risk of ischemia-reperfusion injury, rapid systemic drug clearance, and inadequate targeting efficiency. To address these limitations, we developed an innovative multifunctional biomimetic nano-therapeutic system (PDMC/CHP@S/V), which comprised a triblock copolymer synthesized through controlled polymerization of dopamine methacrylamide (DMA), 2-methacryloyloxyethyl phosphorylcholine (MPC), and methacrylate-functionalized cyclodextrin. Adamantane-conjugated cardiac homing peptide was anchored onto the nanoparticles surface via host-guest interactions, enabling precise infarct-targeting drug delivery. The cell membrane-like MPC shell markedly reduced macrophage-mediated phagocytosis, prolonging circulation time, while the catechol groups in DMA provided robust antioxidant effects by scavenging reactive oxygen species (ROS). The in vitro experiments showed that the nanosystem effectively reduced ROS level, alleviated cell apoptosis, and restored mitochondrial membrane potential in hypoxic cardiomyocytes. Furthermore, the in vivo tests indicated that PDMC/CHP@S/V significantly improved cardiac function, as evidenced by a remarkable reduction of myocardial fibrosis and attenuated ventricular remodeling in AMI mice model. Collectively, the spatiotemporal collaborative delivery design of PDMC/CHP@S/V nanoparticles addressed limitations of conventional therapies, providing an efficient, stable, and safe strategy for achieving targeted AMI treatment.
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ACS Nano
·
2026
·
IF 16
Xuan Zhao,Ruilong Shao,Yiruo Yu,Zhiwen Hu,Yiting Jiang,Yilan Zhou,Zibiao Zhong,Jinping Zhou
Sepsis is a life-threatening inflammatory syndrome caused by bacterial infections, with limited therapeutic options in critical care. Lipopolysaccharide (LPS) from Gram-negative bacteria triggers sepsis by activating toll-like receptor 4 (TLR4) through myeloid differentiation protein-2 (MD-2) binding. The phosphate groups in LPS serve as key recognition elements for this interaction. This study developed a zirconium/cerium (Zr/Ce) dual-metal nanozyme with phosphatase activity that catalyzes LPS dephosphorylation, disrupting LPS–TLR4 binding to inhibit NF-κB signaling and reduce inflammation. The nanozyme incorporated l-arginine and tannic acid (TA) to enhance the LPS binding capacity, while TA modification improved the antioxidant capability, constructing a multifunctional TA-Zr/Ce nanozyme. Results show that the nanozyme effectively catalyzes LPS dephosphorylation and suppresses inflammatory cytokine release from macrophages. In septic mice, TA-Zr/Ce treatment improved organ function, reduced systemic inflammation, and increased survival rates while promoting anti-inflammatory M2 macrophage polarization. Furthermore, this study constructed a regenerated cellulose microsphere (RCM)-based hemoperfusion system for loading nanozymes and efficiently removing LPS from blood, demonstrating strong clinical translation potential. Therefore, this research provides additional approaches and tools for the clinical management of sepsis.
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ACS Sustainable Chemistry & Engineering
·
2026
·Vol. 14
·期 30
·13777-13787
·
IF 7.6
Liu Jupen,Wang Rongzheng,Zhu Shunfeng,Bao Siyu,Wang Qian,Pang Xiangchao,Lu Zhe,Yu You
Microgels exhibit excellent toughening capabilities for the construction of strong and resilient hydrogels; however, their fabrication often involves complex procedures, sophisticated architectures, or biocompatibility-compromising metal catalysts. To address these limitations, we develop a metal-free orthogonal photochemical strategy that employs organic riboflavin to prepare porous double-network microgels for the fabrication of tough and printable hydrogels. This metal-free system demonstrates photochemical performance comparable to that of conventional ruthenium-based catalysts. Under light irradiation, riboflavin rapidly and orthogonally triggers free-radical polymerization of vinyl monomers and covalent crosslinking via phenolic coupling, while simultaneously enabling controlled Ca 2+ release for ionic crosslinking (within several tens of seconds). The resulting microgels possess a synergistic architecture comprising a double-network structure, which imparts exceptional energy dissipation, and an internal porous structure that enhances entanglements. When incorporated as reinforcing fillers into the hydrogel matrix, these microgels endow the bulk hydrogels with superior mechanical performance, achieving a fracture energy of ∼500 J m –2 and a toughness of ∼380 kJ m –3 . Furthermore, they confer pronounced shear-thinning characteristics and rapid self-recovery to the precursor inks. As a result, high-fidelity 3D printing of intricate architectures with feature sizes down to ∼100 μm is readily achieved, as demonstrated by a customizable pressure sensor array capable of discriminating both applied loads and object shapes. This work offers a biocompatible, efficient, and versatile strategy for preparing advanced microgel tougheners and bulk hydrogels, paving the way toward printable, tough hydrogels for a broad range of applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yan-Ping Lin,Dong-Yan Zhou,Shang-Guang Du,Zi-Fan Yu,Rui-Na Zhou,Hao-Miao Li,Wei-Ming Chai
Protocatechualdehyde (PCA) is a better PPO inhibitor than ellagic acid. • PCA causes structural stretching and conformational change of PPO. • PCA is demonstrated as a potent anti-browning agent. • PCA regulates phenolic metabolism, reduces lipid peroxidation, boosts AsA-GSH cycle. • PCA regulates cell wall metabolism, inhibiting senescence-related browning. Polyphenol oxidase (PPO) is a vital polyphenol-metabolizing enzyme responsible for the enzymatic browning of fruits and vegetables. This study assessed the anti-PPO activity of protocatechualdehyde (PCA), yielding an IC 50 value of 94 μM (95% CI: 91.8 to 96.4 μM). Kinetic analysis revealed that PCA acted as a reversible and competitive inhibitor of PPO. Molecular docking indicated that hydrogen bonding and hydrophobic interactions were the main driving forces for PCA-PPO binding. Fluorescence spectroscopy further demonstrated that PCA binding altered the microenvironments around tyrosine and tryptophan residues, leading to conformational rearrangements and partial unfolding of the enzyme, evidenced by significant fluorescence quenching. Molecular dynamics simulation demonstrated that PCA perturbed the secondary structure of PPO and caused stretching of its overall structure, thereby inhibiting the catalytic activity of the enzyme. Furthermore, PCA demonstrated excellent anti-browning activity by regulating phenolic metabolism, reducing membrane lipid peroxidation, enhancing the ascorbic acid-glutathione cycle, and modulating cell wall metabolism to delay cellular senescence. Collectively, these findings offer some valuable insights into the development of PPO inhibitor and anti-browning agent, and provide a theoretical foundation for the potential application of PCA in the area of food preservation. Download: Download high-res image (249KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yue Tang,Zhen Wu,Jiali Chen,Xiaojuan Niu,Lian Xia,Yuangen Wu
Bio-derived FAD acts as a small-molecule light-driven oxidase mimic. • FAD exhibits wavelength-selectivity by responding only to blue and UV light. • Oxygen-dependent blue-light-driven and oxygen-independent UV-driven modes. • H 2 O 2 -free, metal-free colorimetric sensor achieves visual detection of nitrite. • A sensor enables sensitive detection with precise spatiotemporal control. Natural enzymes are constrained by poor stability and structural fragility, while existing enzyme mimics predominantly exhibit an “always-on” mode lacking spatiotemporal controllability. Herein, it was discovered that bio-derived flavin adenine dinucleotide (FAD), the core cofactor of natural photoenzymes, exhibits intrinsic light-driven oxidase-mimicking activity under blue and ultraviolet (UV) light. Notably, FAD operates in an oxygen-dependent mode under blue light and an oxygen-independent mode under UV light, enabling its application in various complex scenarios. Leveraging the superior biocompatibility and application potential of the blue-light mode, a novel colorimetric sensor for nitrite detection was successfully constructed. FAD catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB under blue light, and nitrite triggers the diazotization of oxTMB to generate yellow diazo-TMB, enabling visual detection for nitrite through color change. The colorimetric sensor achieves a wide linear range of 40–440 μM, with a low limit of detection (LOD) of 1.93 μM. Moreover, the sensor exhibits excellent anti-interference capability and satisfactory recoveries (85.0%–103.5%) in real water samples. This work establishes FAD as a bio-derived light-driven oxidase mimic, providing a new strategy for designing efficient, wavelength-selective enzyme mimic and opening a new avenue for advanced light-driven sensors with precise spatiotemporal control. A facile strategy employs bio-derived flavin adenine dinucleotide (FAD), which exhibits intrinsic light-driven oxidase-mimicking activity, enabling the construction of a metal-free, spatiotemporally controllable colorimetric sensor for nitrite detection. Download: Download high-res image (105KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）