MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qi Liang,Tao Liao,Liqin Zhu,Changxiang Liu,Qian Liu,Jianhua Xiong,Ruiping Deng,Xiaoning Liao
A novel nanocomposite consisting of core (Cu)-shell (Ag) nanoparticles encapsulated in carbon nanotubes (CTs) grown on a MOF-derived carbon framework was synthesized by in-situ pyrolyzing method. After further modification with multi-walled carbon nanotubes (MWCNTs), a hierarchical porous carbon network confined core (Cu)-shell (Ag) bimetal (Cu@Ag@CTs/MWCNTs) with excellent catalytic properties to isoproturon (ISO) and norfloxacin (NOR) was obtained. The electrochemical sensor based on the Cu@Ag@CTs/MWCNTs can simultaneously detect the ISO and NOR with excellent sensitivity, good selectivity and perfect stability as well as a separation of peak potential of 270 mV. The calibration curves for ISO and NOR achieved by the proposed sensor range from 80 nM to 18 μM and 7.0 nM to 1.7 μM with detection limits of 26 nM and 2.0 nM, respectively. Moreover, the sensor shows good recoveries in simultaneous detection of ISO and NOR in real sample analysis, highlighting its high feasibility in practical application.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Qin Zhou,Yushi Wei,Yijing Liao,Deming Gong,Guowen Zhang
This study investigated the inhibitory effect of vitamin B6 (VB6) on α-glucosidase (Glu) and its synergistic inhibition mechanism with liquiritigenin (LTG). VB6 was identified as a weak mixed-type inhibitor of α-Glu (IC 50 = 2.76 ± 0.95 mM). VB6 quenched the endogenous fluorescence mainly through hydrogen bonding, reducing surface hydrophobicity and loosening the structure of α-Glu. Furthermore, the combination of 6 μM LTG and 0.5 mM VB6 produced the strongest synergistic inhibition on α-Glu without changing the mixed-inhibition pattern. Computer simulation results showed that VB6 reversibly occupied the allosteric cavity of α-Glu, causing structural rearrangements and conformational changes, which allowed LTG to penetrate deeper into the active center, forming hydrogen bonds with the residues Asp69, Asp352 and Arg442, thereby enhancing LTG's binding affinity and inhibitory effect. These findings provided the potential of VB6 in enhancing LTG's anti-α-Glu activity and a basis for the formulation design of hypoglycemic functional food.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 18
·4401-4410
·
IF 4.3
Yuan Kaisong,Huang Rui,Gong Kaishuo,Xiao Ziyi,Chen Jialin,Cai Siyao,Shen Jiayi,Xiong Zuer,Lin Zhexuan
Polarized light microscopy (PLM) is a common but critical method for pharmaceutical crystallinity characterization, which has been widely introduced for research purposes or drug testing and is recommended by many pharmacopeias around the world. To date, crystallinity characterization of pharmaceutical solids is restricted to laboratories due to the relatively bulky design of the conventional PLM system, while little attention has been paid to on-site, portable, and low-cost applications. Herein, we developed a smartphone-based polarized microscope with an ultra-miniaturization design (“hand-held” scale) for these purposes. The compact system consists of an optical lens, two polarizers, and a tailor-made platform to hold the smartphone. Analytical performance parameters including resolution, imaging quality of interference color, and imaging reproducibility were measured. In a first approach, we illustrated the suitability of the device for pharmaceutical crystallinity characterization and obtained high-quality birefringence images comparable to a conventional PLM system, and we also showed the great promise of the device for on-site characterization with high flexibility. In a second approach, we employed the device as a proof of concept for a wider application ranging from liquid crystal to environmental pollutants or tissues from plants. As such, this smartphone-based hand-held polarized light microscope shows great potential in helping pharmacists both for research purposes and on-site drug testing, not to mention its broad application prospects in many other fields. Graphical Abstract
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JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Tong Chen,Shuya Wang,Xufang Zong,Bingxiang Li,Ye Shu,Xinyuan Di,Wanning Zhu,Guangsan Song,Jun Jiang
Periodic mesoporous organosilicas (PMO) hydrophilic microspheres were synthesized by co-condensation of sulfated polysaccharide from Lilum lancifolium Thunb. bridged silane (SLLTPBS) and polyhedral oligomeric silsesquioxane (POSS) as stationary phase (PMO(SLLTP-POSS)) for per aqueous liquid chromatography (PALC), which would overcome the disadvantages of using a large amount of acetonitrile on the hydrophilic interaction liquid chromatography (HILIC) columns. Average particle size of PMO (SLLTP-POSS) microspheres was 4.9 μm, which was suitable for stationary phase. The retention mechanism of the stationary phase in PALC was mainly hydrophobic interactions and also included some ion-exchange interactions and electrostatic interactions. The acid-base resistance was greatly improved compared to the C18 column. The PMO(SLLTP-POSS) column under PALC mode had increased the resolution when separating some hydrophilic compounds such as eight organic acids and eleven sweeteners compared with the C18 column and HILIC column. The new column was more efficient than the HILIC columns. Additionally, a PALC-triple quadrupole mass spectrometry approach for the simultaneous identification of the eleven sweeteners was developed. The averagere coveries of the eleven compounds were 70.20%–91.33% with the relative standard deviation (RSD) range of 1.74% to 4.27%. The results showed good precision and accuracy of the method.
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Foods
·
2022
·Vol. 11
·期 21
·3388
·
IF 5.561
Xijuan Tu,Yunmin Tao,Jiaxu Chen,Chunping Du,Qian Jin,Yuchang He,Ji Yang,Shaokang Huang,Wenbin Chen
In the present study, a lanthanide fluorescence sensor array was developed for the discrimination of honey’s botanical origin. Dipicolinic acid (DPA) was used as the antenna ligand for sensitizing the fluorescence of Tb3+and Eu3+to prepare the DPA-Tb3+/Eu3+complex. This lanthanide fluorescence sensor showed a cross-reactive response to the major constituents of honey, which led to the result that different classes of honey solution exhibited distinct quenching effects on the fluorescence of the DPA-Tb3+/Eu3+complex. Furthermore, a fluorescence sensor array composed of ten sensors was constructed by adjusting the pH and the component of the DPA-Tb3+/Eu3+complex to show multivariate responses towards honey. The visual fluorescence image of the sensor array was recorded by using a smartphone under excitation with portable UV lamp. Results indicated that the pattern of the visual image was related with the botanical origin. After extracting the RGB value of each sensor in 96-well plate, the ratio of R/G was used for principal component analysis (PCA). The results showed that three classes of honey (astragalus, logan, and litchi) were well distinguished. Moreover, the value of principal component 1 (PC1) showed good linearity with the composition of mixing honey and could be used for semi-quantitative analysis. The proposed lanthanide fluorescence sensor array presents a visual and portable method for the discrimination of a honey’s origin without the use of analytical instruments, and might provide a novel and simple strategy for the measurement of food origin.Keywords:honey;botanical origin;lanthanide complex;fluorescence;sensor array
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Zhi-feng Cai,Hao-yang Li,Xian-song Wang,Can Min,Jia-qi Wen,Rui-xin Fu,Zi-yue Dai,Jing Chen,Ming-zhu Guo,Hui-juan Yang,Ping-ping Bai,Xiao-mi Lu,Tao Wu,Ying Wu
Polyethyleneimine (PEI), playing roles as a templating agent, has been successfully applied to prepare blue-green-emitting copper nanoclusters (Cu NCs@PEI) on the basis of a rapid chemical reduction synthesis method. Some characterization methods, such as fluorescence spectrophotometer , UV spectrophotometer, Fourier transform infrared instrument (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron microscope (TEM) and fluorescence lifetime , were used for the analysis of physical, chemical and optical properties of Cu NCs@PEI. The Cu NCs@PEI displayed a blue-green emission wavelength at 485 nm and the corresponding quantum yield ( QY ) was 2.24%. Subsequently, the as-synthesized Cu NCs@PEI were applied for the trace analysis of oxytetracycline on the basis of the inner filter effect (IFE) and static quenching, which caused the fluorescence to be effectively quenched. Additionally, this proposed platform based on the Cu NCs@PEI for oxytetracycline sensing possessed an excellent linear range from 0.5 to 300 μM with a lower detection limit of 0.032 μM ( S/N = 3) and correlation coefficient R 2 of 0.9972. Surprisingly, as the temperature increased, the fluorescence intensities of Cu NCs@PEI decreased gradually. Thence, the Cu NCs@PEI could be applied for the sensing of temperature. Finally, the practicability of the detection method have been successfully verified by analyzing oxytetracycline in real samples, enlarging its applicable fields for oxytetracycline sensing.
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3 Biotech
·
2022
·Vol. 12
·期 9
·1-15
·
IF 2.893
Weng Chunyue,Mi Zheyan,Li Meijing,Qin Haibin,Hu Zhongce,Liu Zhiqiang,Zheng Yuguo,Wang Yuanshan
To improve S-Adenosyl-L-methionine (a compound with important physiological functions, SAM) production, atmospheric and room temperature plasma and ultraviolet-LiCl mutagenesis were carried out with Saccharomyces cerevisiae strain ZY 1–5. The mutants were screened with ethionine, L-methionine, nystatin and cordycepin as screening agents. Adaptive evolution of a positive mutant UV6-69 was further performed by droplet microfluidics cultivation with ethionine as screening pressure. After adaptation, mutant T11-1 was obtained. Its SAM titer in shake flask fermentation reached 1.31 g/L, which was 191% higher than that of strain ZY 1–5. Under optimal conditions, the SAM titer and biomass of mutant T11-1 in 5 L bioreactor reached 10.72 g/L and 105.9 g dcw/L (142.86% and 34.22% higher than those of strain ZY 1–5), respectively. Comparative transcriptome analysis between strain ZY 1–5 and mutant T11-1 revealed the enhancements in TCA cycle and gluconeogenesis/glycolysis pathways as well as the inhibitions in serine and ergosterol synthesis of mutant T11-1. The elevated SAM synthesis of mutant T11-1 may attribute to the above changes. Taken together, this study is helpful for industrial production of SAM.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 101
·期 15
·6271-6280
·
IF 3.639
Naymul Karim,Mohammad Rezaul Islam Shishir,Tao Bao,Wei Chen
BACKGROUND Shiitake mushroom is one of the most popular delicious vegetables, although fresh shiitake mushroom has short shelf life as a result of biochemical degradation. Drying can prolong the shelf life of mushroom. Additionally, application of cold plasma pretreatments (CPT) before drying can preserve the product quality, processing costs and nutritional values. Therefore, we aimed to explore the effect of cold plasma pretreated hot-air drying at 50, 60 and 70 °C on the physicochemical characteristics, nutritional values and antioxidant activity of shiitake mushroom. RESULTS Scanning electron microscopy micrographs showed that CPT induced the surface modification of fresh shiitake (such as cellular disarrangement, cellular shrinkages, disruption or break down of cell walls, and intracellular spaces and cavities) and facilitate the rapid drying than control samples. Furthermore, CPT improved the powder qualities (bulk density, water retention and swelling index) and preserved higher nutritional attributes (sugars, vitamins, phenolic acids contents and antioxidant activity) compared to the control groups. CONCLUSION Conclusively, CPT could be a suitable alternative technique for improving drying characteristics and preserving nutritional attributes of agro-based products. © 2021 Society of Chemical Industry.
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MACROMOLECULAR RAPID COMMUNICATIONS
·
2020
·Vol. 41
·期 24
·2000441
·
IF 4.886
Yue Zhao,Zuhao Li,Qiuju Li,Longfei Yang,Hou Liu,Ruyue Yan,Lizhi Xiao,He Liu,Jingcheng Wang,Bai Yang,Quan Lin
Diabetic foot ulcers (DFU) remain a very considerable health care burden, and their treatment is difficult. Hydrogel-based wound dressings are appealing to provide an optimal environment for wound repair. However, the currently available hydrogel dressings still need surgical or mechanical debridement from the wound, causing reinjury of the newly formed tissues, wound infection, delayed healing time, and personal suffering. Additionally, to meet people's increasing demand, hydrogel wound dressings with improved performance and multifunctionality are urgently required. Here, a new multifunctional supramolecular hydrogel for on-demand dissolvable diabetic foot wound dressings is designed and constructed. Based on multihydrogen bonds between hydrophilic polymers, the resultant supramolecular hydrogels present controlled and excellent properties, such as good transparency, antibacterial ability, conductive, and self-healing properties. Thus, the supramolecular hydrogels improve the new tissue formation and provide a significant therapeutic effect on DFU by inducing angiogenesis, enhancing collagen deposition, preventing bacterial infection, and controlling wound infection. Remarkably, the resultant hydrogels also exhibit stimuli-responsive ability, which renders its capability to be dissolved on-demand, allowing for a facile DFU dressing removal. This multifunctional supramolecular hydrogel may provide a novel concept in the design of on-demand dissolvable wound dressings.
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DYES AND PIGMENTS
·
2020
·
IF 4.613
Xiuling Ji,Suping Wang,Yiyong Luo,Xiaotian Yuan,Yunlin Wei,Qi Zhang,Kunhao Qin,Yujiao Tu
Due to small size, strong fluorescence and low cytotoxicity, fluorescent carbon dots (CDs) attract people's attention. The CDs- Ws derived from Weissella sp. Kl-3 with bright blue photoluminescent were obtained via hydrothermal method . The CDs- Ws could selectively label dead bacterial and yeast cells, and show the potential for the differentiation of live/dead microbe. It showed that the prepared CDs- Ws had multicolor fluorescence emission properties, excellent biocompatibility and low biotoxicity with cell and in vivo imaging. In addition, CDs- Ws sensitized by ampicillin sodium was employed as fluorescence nanosensor (CDs- Ws -Amp Na) to detect Cr(VI) and VB 12 based on inner filter effet mechanism. The linear correction was obtained for VB 12 detection in 0–25 μM with 51.50 nM detection limit, and the linear range was 0–50 μM with 106.57 nM detection limits for Cr(VI) by CDs- Ws -Amp Na. The fluorescent nanosensor was used for detection of Cr(VI) in water and VB 12 in serum and milk. It shows excellent potential in bioimaging , Cr(VI) and VB 12 detection.
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OPTIK
·
2020
·
IF 2.187
Siliang Liu,Chunyu Liu,Yu Ren,Shangqi Kuang,Chengcheng Zhou,Hongxing Cai,Zhihai Yao
The chicken and skin were studied by fluorescence spectroscopy . The three-dimensional fluorescence spectra of chicken and skin were measured, It was concluded that the optimal excitation wavelength corresponding to the fluorescence peak of 330 nm in chicken was 270 nm, and that of 475 nm and 520 nm was 370 nm. The optimal excitation wavelength corresponding to the 475 nm fluorescence peak of chicken skin was 370 nm. The fluorescence spectra of chicken skin and chicken excited by the wavelength of 370 nm were analyzed. The fluorescence peak came from myoglobin at 555 nm at the beginning of roasting, and the contribution of fluorescence peak came from oxygenated myoglobin at 540 nm and 580 nm as the baking time increased. When the baking time was more than 12 min, the fluorescence intensity of oxygenated myoglobin at 580 nm was close to zero. At this point, a considerable part of myoglobin had been converted into ferric myoglobin, while Fe 3+ in ferric myoglobin could not be absorbed by human body. At this point, the skin of chicken was no longer suitable for large amounts of consumption, while the relative performance of chicken was not obvious.
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