Huan Zhou,Ying Yang,Mengmeng Yang,Wenjie Wang,Yaping Bi
Mesoporous hydroxyapatite is widely applied in biomedical and environmental fields due to its high surface area and adsorption capacity. In current work a one-step hydrothermal method to synthesize mesoporous HA was developed with the assistance of a cost-effective template vitamin C. It was observed the pore size and morphology of hydrothermally synthesized HA were correlated to applied solution composition. A combination of calcium hydroxide, orthophosphate, and vitamin C can finally result in mesoporous rod-like HA nanoparticles with surface area of 88 m 2 .g −1 and pore size of 15.7 nm on average. The mesoporous HA exhibited enhanced adsorption of model drug doxorubicin in comparison to conventionally synthesized HA as expected. Consequently, this work provides new pathway to prepare mesoporous HA with advantages of economy and convenience.
Switching aerobic respiration to anaerobic glycolysis of cancer cells plays an important role in development and progression of acquired resistance. Since vitamin C enabled the inhibition of glycolysis of cancer cells, and erlotinib-resistant sub-line of HCC827 (ER6 cells) switched its metabolic features to higher glycolysis for survival, we hypothesize that vitamin C is able to inhibit glycolysis of ER6 cells. In this study, we found that both reduced vitamin C and oxidized vitamin C (DHA) could selectively suppress the viability of ER6 cells. DHA was efficient in inhibiting glycolysis and leading to energy crisis, which could be one mechanism for overcoming drug resistance to erlotinib of ER6 cells. Our data suggest that applying DHA could be a novel treatment strategy for NSCLC with acquired resistance to targeted therapy.
Fast curing hydrogels are highly valuable in the skin care industry. Multi-arm polyethylene glycol (PEG) is a commonly used crosslinking agent for the preparation of fast curing hydrogels. However, the hydrogel preparation using multi-arm PEG crosslinking agents usually requires the end group modifications of reactants, which makes the preparation process complicated and produces hydrogels with low curing rates. In the present study, a fast in situ curing hydrogel, 4-arm-PEG-succinimidyl carbonate/carboxymethyl chitosan (PEG-SC/CMCS), was prepared using 4-arm-PEG-SC as the crosslinking agent and carboxymethyl chitosan (CMCS), a derivative of natural polysaccharide with excellent comprehensive properties, as the framework without any end groups modified. The structure and properties of the prepared hydrogel were characterized by FTIR, XRD, SEM, rheology and swelling kinetics. The effects of reaction conditions on the structure and properties of hydrogel were investigated. The PEG-SC/CMCS hydrogel exhibited pH-sensitive swelling behaviors. Its application potential in skin care products is demonstrated with its excellent sustained-release performance of Vitamin C, a model drug for skin treatment, and high adsorption capacity of heavy metal ions that are easily deposited on skin surface. Our work has provided a new strategy for building multifunctional gel materials for skin care and offered a PEG-SC/CMCS hydrogel with great application potentials.
危险品化学品经营许可证（带存储）