POULTRY SCIENCE
·
2026
·Vol. 105
·期 5
·106672
·
IF 4.5
Guangxiao Zhang,Yawen Sun,Ling He,Bowen Zhao,Hongchao Jiao,Jingpeng Zhao,Xiaojuan Wang,Min Liu,Kelin Li,Hai Lin,Haifang Li
Vitamin D 3 (VD 3 ) is an essential fat-soluble micronutrient that regulates diverse biological processes. However, its long-term impact on avian intestinal development remains largely unknown. In this study, we detected that the VD 3 content was significantly lower in late-cycle production eggs (68 weeks old, termed “LP eggs”) compared to the peak-cycle production eggs (44 weeks old, named “PP eggs”) ( P < 0.05). Notably, the intestine index was reduced ( P < 0.05), and the intestinal expression of tight junction (TJ) proteins (Occludin and ZO-1) was decreased for chicks hatched from LP eggs versus those from PP eggs ( P < 0.05). Thus serial dosages of VD 3 (2.5, 5.0, or 7.5 μg VD 3 /egg) were injected into the amniotic cavity of embryos hatched from LP eggs on embryonic day 17 (E17). This intervention effectively improved hatchability, promoted intestinal development that persisted through 14-days post-hatch, and showed immuno-modulatory effects manifesting as Interleukin-6 ( IL-6 ) downregulation and Interleukin-10 ( IL-10 ) upregulation. Further in vivo and in vitro analysis revealed that VD 3 improved intestinal development by activating TJ-related genes ( P < 0.05), with vitamin D receptor (VDR) mediating these effects. Chromatin immunoprecipitation (ChIP) assays confirmed that 1,25-(OH) 2 D 3 strengthened VDR binding to promoter regions of Occludin and ZO-1 but decreased the interaction degree of VDR with the IL-6 promoter ( P < 0.05). These findings establish that in ovo injection of VD 3 is an efficacious perinatal intervention strategy, which significantly mitigates developmental constraints in late-phase poultry production through VDR-dependent intestinal barrier potentiation and immuno-homeostasis modulation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Fan He,Qiaoqiao Ma,Huahua Zhang,Junheng Gou,Qingbo Deng,Jinsong Gong,Jinsong Shi,Zhenghong Xu,Hui Li
1α,25-dihydroxyvitamin D 3 (1α,25(OH) 2 VD 3 ), the biologically active form of Vitamin D 3 (VD 3 ), plays a crucial role in a wide range of physiological processes. However, the efficient biosynthesis of 1α,25(OH) 2 VD 3 is hindered by the low activity of natural enzymes, poor solubility of substrate VD 3 , and its intracellular metabolic loss. Herein, we developed an integrated bioprocess by synergizing engineered dihydroxylase (CYP109A2-H) with a deep eutectic solvent (DES) based resting cell system to overcome these limitations. A semi-rational design strategy was employed to enhance the performance of the enzyme, which yielded several beneficial mutants including E81G that increased the catalytic titer by 43.6%. Concurrently, a DES resting cell system was constructed, wherein DES dramatically increased VD 3 solubility and resting cells eliminated metabolic loss. This synergistic strategy achieved a record-high 1α,25(OH) 2 VD 3 space–time yield of 33.15 mg·L −1 ·d −1 , establishing the most efficient single-batch bioconversion reported to date. This work provides a sustainable and efficient platform for 1α,25(OH) 2 VD 3 production and a general strategy for the biohydroxylation of hydrophobic compounds.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xuehui Deng,Xuchen Wang,Wenlong Xiao,Li Song,Qiaofeng Guo,Kai Huang,Nani Wang
The pathogenesis of sarcopenia, a geriatric syndrome associated with chronic glucocorticoid exposure, involves redox homeostasis dysregulation of skeletal muscle. There is an urgent need for targeted therapeutic strategies. In this study, we developed a thermosensitive hydrogel composed of skeletal muscle-derived decellularized extracellular matrix, chitosan, and β-glycerophosphate. This multifunctional platform was co-encapsulated with a nuclear factor erythroid 2-related factor 2 (Nrf2) agonist (bardoxolone) and myoblast-derived exosomes to synergistically enhance muscle regeneration. The injectable hydrogel exhibited rapid sol-gel transition at 37 °C and sustained bardoxolone release kinetics. In dexamethasone (Dex)-induced C2C12 myoblasts, the hydrogel effectively normalized atrophic markers (muscle-specific RING finger protein 1, muscle atrophy F-box protein), while upregulating myogenic factors (myoblast determination protein 1, myosin heavy chain, myosin heavy chain 7, and myogenin). Intramuscular administration of the hydrogel in Dex-treated mice ameliorated sarcopenic manifestations through enhanced skeletal muscle mass, grip strength, weight-loaded swimming time, rotating rod time, and myofiber cross-sectional area. Mechanistically, the hydrogel restored mitochondrial impairment in Dex-treated myoblasts through Nrf2 pathway activation, evidenced by upregulated nuclear Nrf2, translocase of outer mitochondrial membrane 20, voltage dependent anion channel 1, and recovered oxygen consumption rate. Crucially, complete therapeutic efficacy ablation in both in vitro and in vivo Nrf2-knockout models confirmed Nrf2 as the central regulatory hub. This hydrogel integrates extracellular matrix-mimetic architecture, controlled molecular release kinetics, and exosome-mediated cytoprotection, establishing a therapeutic strategy for sarcopenia management.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Shanglin Li,Leiyi Zhang,Yulun Wu,Jiale Guo,Chengbo Yang,Kun Chen,Yajuan Li,Longjiang Tian,Jingbo Liu,Ting Zhang,Zhiyang Du
With the increasing demand for the co-encapsulation and controlled delivery of bioactive compounds, the design of liquid-based carriers has garnered considerable attention. Vitamin D 3 and riboflavin sodium phosphate (RP) suffer from poor stability and low bioavailability; nevertheless, high internal phase Pickering emulsions (HIPPEs) feature a tunable microstructure that allows for effective compartmentalization of multiple components. In this study, HIPPEs stabilized by egg white peptide (EWP)-konjac glucomannan (KGM) co-assemblies were constructed as a versatile vehicle for the co-delivery of vitamin D 3 and RP. EWP-KGM co-assemblies were comprehensively characterized using multispectral techniques and structural analyses. The EWP@KGM co-assembly (mass ratio 10:5) exhibited enhanced emulsifying activity index (EAI: 15.12 m 2 /g, 3.74-fold higher than EWP) and emulsifying stability index (ESI: 736.60 min, 53-fold higher than EWP), attributed to increased interfacial thickness and steric hindrance. All HIPPEs demonstrated shear-thinning behavior, gel-like viscoelasticity (G′ > G″), and long-term storage stability. Under simulated gastrointestinal digestion, EWP-KGM-HIPPEs protected RP integrity and improved vitamin D 3 bioaccessibility. FFA release kinetics followed Korsmeyer-Peppas model, indicating anomalous transport driven by diffusion and matrix relaxation. This study presents a promising platform for the dual encapsulation of bioactives with contrasting polarities, paving the way for applications in nutraceuticals.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Food Research
·
2026
·
IF 6.2
Lihui Yang,Xian’e Ren,Yuting Wu,Feng Yang,Kunming Zhang,Yongchun Huang,Xiaolong Miao
To enhance the bioaccessibility of vitamin D3 in fortified food systems, hydrodynamic cavitation, ultrasonic cavitation, and an ascorbic acid/hydrogen peroxide system were employed to generate free radicals that mediated the formation of gallic acid-soy protein isolate (GA-SPI) conjugates. The resulting conjugates were used to stabilize emulsions. The emulsion properties and their effects on vitamin D3 delivery were investigated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that covalent conjugation between GA and SPI was promoted by free radical generation in all three treatments. Compared with emulsions prepared with soy protein isolate alone, significant improvements were observed for emulsions stabilized by GA-SPI conjugates, including smaller particle size, higher absolute zeta potential, higher storage modulus, and improved storage stability and antioxidant capacity. These improvements were attributed to GA incorporation and the processing methods. More importantly, when used to encapsulate vitamin D3, bioaccessibility ranged from 53.36 ± 0.57% to 80.06 ± 0.12% for GA-SPI stabilized emulsions prepared by these methods, compared with the untreated group. These findings confirm that emulsion delivery performance can be improved by the three free radical-mediated treatments through promotion of polyphenol-protein conjugation.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Agriculture and Food Research
·
2026
·
IF 6.2
Jiaxin Lin,Tao Wang,Ren Wang,Wei Feng,Xiaohu Luo,Kai Huang,Jian He
Vitamin K 2 (VK 2 ) and vitamin D 3 (VD 3 ) can synergistically promote calcium absorption and reduce the prevalence of osteoporosis. Given their hydrophobicity and environmental sensitivity, stabilizing both these vitamins is pivotal for their application. In this study, we designed shellac-gliadin composites (SGCs) with favorable amphiphilicity, yielding a self-emulsifying delivery system (SEDS) upon mild mixing with eugenol. By increasing eugenol/SGCs mass ratio, SEDS was transformed from spherical to a network-like structure, forming tunable topological nanostructures with tailorable delivery performance and bioavailability. Based on the dual-step process, VK 2 was encapsulated during shellac-gliadin assembly, while VD 3 was co-encapsulated via self-emulsification. The generated dual-vitamin self-emulsifying system (DSEDS) improved the stability under UV-light and long-term storage for both vitamins with high encapsulation capacities of 48.05 and 9.36 mg/g for VK 2 and VD 3 , respectively. The dual-step strategy introduced a novel approach for loading various fat-soluble nutrients, with potential applications in food and nutrition industry.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Xia Ke,Kai-Rui Wang,Tong Zheng,Ke-Qiang Xu,Ji-Dong Shen,Jun-Ping Zhou,Zhi-Qiang Liu,Yu-Guo Zheng
Fungal unspecific peroxygenases (UPOs) can directly utilize H2O2 as an oxygen and electron donor for selective C–H bond oxyfunctionalization. However, long-time duration of genetic modification and expression in Pichia pastoris limited its quick evolution. Herein, we propose a novel structure-guided combinatorial evolution strategy(SBPCS) to accelerate the iterative cycle of multipoint mutagenesis for C25 hydroxylation of VD3 by AaeUPO. Through mutational screening of key residues within the substrate-binding pocket, combined with molecular dynamics (MD) simulations, we observed shortened distance and a stabilized dihedral angle between the substrate’s C25 atom and the heme Fe═O center. After two rounds of coevolution, a triple mutant (G195A/V244I/S272A, M3) was successfully obtained, demonstrating a 5.16-fold increase in C25-hydroxylation efficiency, with a increased the yield of 25(OH)VD3 from 45.8 to 354.40 mg/L. Our study elucidate how the geometry of the heme-VD3 complex effect catalytic efficiency during selective hydroxylation and guided a rational design for accelerating AaeUPO coevolution toward VD3 C25-hydroxylation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying-zhuo Shen,Zheng Wang,Kangping Ning,Chuanli Ren,Dandan Yang,Xiao-Ya Hu,Qin Xu
A highly sensitive and selective split-type perovskite-based photoelectrochemical (PEC) platform was developed for measuring alkaline phosphatase (ALP) activity in milk and serum samples. ALP in the test sample hydrolyzed 2-phosphate sesquimagnesium salt hydrate (AAPS) in a 96-microwell plate to produce ascorbic acid (AA), a PEC electron donor. The resulting AA, which could preferentially annihilate the photogenerated holes, indirectly reflects ALP activity. The PEC used a cetyltrimethylammonium bromide (CTAB)-functionalized CH 3 NH 3 PbI 3 ( [email protected] 3 NH 3 PbI 3 ) film as the cathode to monitor the controlled AA production. Due to the excellent photoelectric characteristics of the CH 3 NH 3 PbI 3 perovskite and the split-type assay, excellent sensitivity and selectivity for ALP detection were obtained. Under the optimum experimental conditions, ALP activity with a limit of detection (LOD) of 2.6 × 10 −4 U/L in a linear dynamic range of 10 −3 ∼ 10 2 U/L was obtained. With its sensitive, rapid, and high-throughput detection capabilities, this split-type and label-free PEC platform has great potential for use in food and biomedical analysis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Jun Zheng,Xianwen Song,Zhaoyu Yang,Yun Tan,Chao Yin,Jiafu Yin,Yufang Lu,Yufan Yang,Chuntai Liu,Lunzhao Yi,Yi Zhang
Micellar hydrogels formed by direct self-assembly of natural compounds have attracted widespread attention as delivery systems owing to their distinctive structures, excellent biocompatibility and simple preparation methods. Herein, we report on glycyrrhizic acid (GA), which is directly self-assembled into micellar hydrogels to encapsulate curcumin (Cur) through non-covalent interactions without excipients or structural modifications. Owing to their reversible non-covalent interactions, the micellar hydrogels have outstanding shear-thinning, self-healing ability and temperature-responsive behavior. Notably, the mechanical properties and release rates of the micellar hydrogels can be easily tuned by adjusting the concentration of GA. Furthermore, the [email protected] micellar hydrogels have exhibited superior stability, remarkable biocompatibility and better antioxidant properties than free Cur, benefiting from improved solubility and stability. In brief, this micellar hydrogel inherits the advantages of both micelles and hydrogels: it has characteristics of micelles, naturally encapsulates Cur and has the ability of hydrogels to control long-term release. We believe this study will offer an innovative strategy to deliver Cur efficiently.
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Weijing Feng,Yintong Teng,Qingping Zhong,Yangmei Zhang,Jianwu Zhang,Peng Zhao,Guoqing Chen,Chunming Wang,Xing-Jie Liang,Caiwen Ou
As the prevalence of vascular calcification (VC), a strong contributor to cardiovascular morbidity and mortality, continues to increase, the need for pharmacologic therapies becomes urgent. Sodium thiosulfate (STS) is a clinically approved drug for therapy against VC; however, its efficacy is hampered by poor bioavailability and severe adverse effects. Plant-derived extracellular vesicles have provided options for VC treatment since they can be used as biomimetic drug carriers with higher biosafety and targeting abilities than artificial carriers. Inspired by natural grapefruit-derived extracellular vesicles (EVs), we fabricated a biomimetic nanocarrier comprising EVs loaded with STS and further modified with hydroxyapatite crystal binding peptide (ESTP) for VC-targeted delivery of STS. In vitro, the ESTP nanodrug exhibited excellent cellular uptake capacity by calcified vascular smooth muscle cells (VSMCs) and subsequently inhibited VSMCs calcification. In the VC mice model, the ESTP nanodrug showed preferentially the highest accumulation in the calcified arteries compared to other treatment groups. Mechanistically, the ESTP nanodrug significantly prevented VC via driving M2 macrophage polarization, reducing inflammation, and suppressing bone-vascular axis as demonstrated by inhibiting osteogenic phenotype trans-differentiation of VSMCs while enhancing bone quality. In addition, the ESTP nanodrug did not induce hemolysis or cause any damage to other organs. These results suggest that the ESTP nanodrug can prove to be a promising agent against VC without the concern of systemic toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Xuyuan Li,Sihan Zhang,Xiaohu Luo,Ren Wang,Wei Feng,Hao Zhang,Zhengxing Chen,Tao Wang
In this study, carboxymethyl cellulose (CMC) and wheat glutenins (WGs) formed complex structures (CWs) featuring nanoparticles (∼160 nm) fixed at the junctions of fibril networks (50 nm in thickness) with numerous smaller nanoparticles attached to the fibril trunks. The complexation was performed by dissolving CMC and WGs in an alkaline solution (pH 12.0) followed by neutralization. Fluorescence study and Fourier transform infrared spectroscopy indicated that electrostatic forces initiated the binding of WGs to CMC, while hydrophobic attractions contributed to the growth of WG nanoparticles on CMC fibrils. This enabled sufficient dispersing of WGs that can be harnessed as binding sites for vitamin D 3 (VD 3 ). Importantly, the microstructures of CWs can be delicately controlled by the substitution degree (DS) of carboxymethyls. Consequently, the stability of VD 3 against long-term storage and UV radiation by CWs was improved by 77.87–92.55% and 113.13–166.45%, respectively, which were highly dependent on DS. Furthermore, the CWs can realize sustained release of VD 3 in simulated gastrointestinal fluid controlled by varied DS. This study provided a facile and effective approach for advanced utilization of WGs with potential applications in the development of novel food structures and formulation of functional ingredients.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）