Chinese Medicine
·
2026
·Vol. 21
·期 1
·196
·
IF 7.4
Huang Yiqing,Zhao Yihang,Wei Jichang,Lu Jihui,Wang Zhiwei,Li Gen,Xu Helong,Li Yuanhua,Huang Xuemei,Wang Penglong
Background Scutellariae Radix - Coptidis Rhizoma herb pair is a classic traditional Chinese medicine (TCM) combination with heat-clearing, dampness-drying and detoxifying effects, extensively applied clinically against bacterial infections. Traditional water decoction and ethanol pretreatment are mainstream extraction approaches, whereas existing comparisons merely emphasize quantitative variations of bioactive compounds, neglecting the underlying supramolecular assembly differences of effective substances. Comparative analysis of solvent-induced supramolecular variations provides a novel perspective to interpret differential pharmacodynamic material bases of TCM formulas. Methods Macroscopic and microscopic morphologies of HH-0 and HH-50 were firstly characterized by visual inspection, SEM and DLS. Their phytochemical profiles were subsequently analyzed by UHPLC-Q-Orbitrap HRMS and HPLC. Antibacterial activities against MRSA were further quantified via turbidimetry and plate assays. UV, FT-IR and MD simulations revealed divergent supramolecular assembly behaviors. Finally, untargeted metabolomics and assembly-peptidoglycan interaction simulations elucidated the underlying anti-MRSA mechanisms. Results The solvent pretreatment endowed HH-0 and HH-50 supramolecules with distinct characteristics. HH-50 featured rougher supramolecular surfaces and higher Zeta potential with favorable dispersibility. The two supramolecules shared consistent active constituents, and HH-50 showed elevated berberine abundance and enhanced anti-MRSA activity. Notably, HH-50 maintained superior antibacterial efficacy and interference against peptidoglycan synthesis and energy metabolism even at identical berberine doses. This confirmed that divergent supramolecular conformations determined antibacterial differences, as diminished hydrogen bonds and weakened van der Waals and electrostatic interactions enhanced the binding affinity between assemblies and MRSA. Conclusion Beyond chemical composition, solvent pretreatment (water versus 50% ethanol) markedly modulates supramolecular assembly behaviors and subsequent assembly-bacteria interactions, thereby regulating the anti-MRSA efficacy of the Scutellariae Radix - Coptidis Rhizoma herb pair. This work emphasizes that supramolecular configurations, rather than simple differences in chemical contents, dominate the pharmacological activities of traditional Chinese medicine preparations. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·646
·
IF 5.4
Li Chunli,Zeng Linyun,Hong Ziyi,Xie Wenjia,Luo Tongxi,Pan Jialiang,Zhang Xinguo
Unique orange-emissive carbon dots (O-CDs) are synthesised via a solvothermal method by safranin T and 2,2’-dithiodibenzoic acid in ethanol. O-CDs show a maximum emission peak at 595 nm and a quantum yield of 81.63% when dissolved in dichloromethane. O-CDs are able to be fabricated into fluorescent hydrogels, powders and films exhibiting excellent fluorescence performance. The linear relationship is observed in the concentration range of 0-200 µM with R 2 of 0.9973, and the limit of detection is 0.19 µM. Meanwhile, this method is effectively utilized to analyze real food samples, such as egg yolk and milk, with recoveries ranging from 99.21% to 101.8%. Sensing mechanism is found to be the inner filter effect and dynamic quenching. Furthermore, O-CDs exhibit low toxicity and nice biocompatibility, facilitating the applications in bioimaging of B16F10-2 cells as well as zebrafish, and enabling VB12 detection in live cells. Therefore, the novel O-CDs developed present broad application prospects including food safety, biosensing, bioimaging and fluorescent films. Graphical
✓ 已验证
产品关联
DOI
高影响因子
Cell Host & Microbe
·
2026
·
IF 23.2
Weijie Wen,Runping Su,Yunyun Liu,Zhiyang Feng,Yancai Li,Jieli Li,Ziyu Huang,Fen Zhang,Shanshan Gao,Yingfei Ma,Qinghua Zhou,Emad M. El-Omar,Jeremy J. Barr,Tao Zuo
The human gut harbors a diverse virome, believed to play a crucial role in maintaining intestinal homeostasis. However, the magnitude of heterogeneity of the gut mucosal virome, and how it differentially regulates host inflammation phenotypes, such as those observed in Crohn’s disease (CD), remains unclear. Here, we identify two mucosal DNA virome enterotypes (E1 and E2) in humans, with E2 subjects exhibiting higher virome diversity and nuanced bacteriophage-bacteria correlations. Moreover, E2-enterotyped CD patients exhibited higher Wulfhauvirus abundance than healthy controls. The E2 virome is causally more proinflammatory in inducing intestinal inflammation in mice. Mechanistically, the novel E2-virome-enriched phage øBTZT001P (belonging to Wulfhauvirus ) lysogenically infects Bacteroides thetaiotaomicron , increasing sphingosine production. Downstream, sphingosine suppresses the commensal bacterium Blautia obeum in the gut, leading to worsened intestinal inflammation. Our findings reveal that configurational differences in the gut virome may dictate disease outcome through a phage-bacteria-metabolite-intestine axis, highlighting the importance of gut phage-bacteria crosstalk in health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 33
·26290-26302
·
IF 6.7
He Ying,Yu Ziyang,Li Qiqiong,Tao Ze,Xu Yushan,Xie Junhua,Nie Shaoping
Bacteroides thetaiotaomicron (Bt)
degrades dietary glycans via carbohydrate-active enzymes (CAZymes),
but their spatial distribution and functional coordination remain
unclear. In this study, we characterized the “labor division”
during glycan degradation across four bacterial fractions: cytoplasm,
outer membrane, extracellular vesicles, and supernatant. All fractions
displayed hydrolytic activity. Proteomic analysis identified abundant
glycoside hydrolases, mainly GH32 enzymes encoded by PUL22, and their
components varied greatly. Untargeted metabolomics identified 45 shared
differential metabolites (e.g., carbohydrates and short-chain fatty
acids), indicating distinct hydrolytic capacities among fractions.
Notably, cross-feeding experiments confirmed that Bt EVs-mediated
degradation can support the growth of recipient bacteria. Collectively,
our results reveal a synergistic multicomponent strategy for glycan
degradation in Bacteroides , offering new insights
into glycan utilization and laying a foundation for developing postbiotics
and functional microbial products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jingyi Wang,Jiaxin Wei,Yuxuan Fu,Qinfei Wan,Jiayi Zhou,Kai Hu,Zisheng Han
Highland barley polysaccharide (HBP) improved lactose metabolism of gut microbiota. • HBP with a lower molecular weight exhibited a stronger improvement impact. • HBP was hugely degraded, especially the xylose residues and Glc p glycosidic bonds. • Segatella , Bifidobacterium , and Ligilactobacillus were obviously enriched. Lactose intolerance affects over 70% of the global population due to the untimely metabolism of lactose in the colon. To accelerate lactose metabolism, we innovatively employed highland barley polysaccharides (HBP) to modulate the colonic fermentation efficiency of lactose using an in vitro fermentation model and fecal samples from lactose intolerant patients. With the addition of HBP, the consumption efficiency of lactose began to increase after 6 h of fermentation. At 48 h, lactose consumption increased by 29%, surpassing the effect of commercial prebiotics. Meanwhile, HBP with lower molecular weight (HBP-L, 8.69 × 10 4 g/Mol) exhibited more effective impacts than HBP with high molecular weight (HBP-H, 1.22 × 10 5 g/Mol), which was particularly evident during the 12–48 h of fermentation. More importantly, HBP, particularly HBP-L, substantially enriched Segatella , Bifidobacterium , and Ligilactobacillus in a time-dependent manner and was positively correlated with decreased lactose levels and elevated bacterial lactase activity, resulting in significant conversion of lactose to lactic acid and short-chain fatty acids. During the first 12 h of fermentation, HBP-L underwent more significant degradation than HBP-H. Meanwhile, the xylose residues and Glc p glycosidic bonds in HBP-L were preferentially utilized, while it was glucose residue in HBP-H. Thus, it indicated that HBPs were degraded and altered the composition of the gut microbiota, thereby enhancing the fermentation efficiency of lactose. Our findings provide a theoretical basis for developing targeted prebiotic foods to manage lactose intolerance Download: Download high-res image (132KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
BMC GASTROENTEROLOGY
·
2026
·
IF 3.2
Yao Jinwen,Gao Xia,Zhu Lin,Wang Linlin,Chen Qian,Liang Weijuan,Feng Huaili,You Ting,Wei Changli,Liu Yuqi,Zhang Hongyang,Chen Chengzhi,Chen Li
Background Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder in childhood, with methylphenidate hydrochloride (MPH) as its first-line pharmacotherapy. Though effective, MPH is associated with underrecognized gastrointestinal adverse events warranting further investigation. Recently, the gut microbiota has attracted attention for treating gastrointestinal distress, but research on its role in alleviating MPH-induced gastrointestinal reactions remains scarce. Methods Herein, Wistar-Kyoto rats (WKY) served as controls alongside spontaneously hypertensive rats (SHR) to explore MPH-induced gastrointestinal adverse drug reactions. The gastrointestinal phenotypes, colonic histopathological characteristics, and inflammatory factor levels were evaluated, and the gut microbiota profiles were analyzed via 16S rRNA gene sequencing. The protective effect of Parabacteroides distasonis ( P. distasonis ) was further verified by supplementing the treated rats with this strain. Results We demonstrated MPH induced diarrhea and colonic inflammatory responses in rats. 16S rRNA gene amplicon sequencing revealed that MPH-treated rats had significantly decreased gut microbiota health index, increased dysbiosis index, and reduced P. distasonis abundance, suggesting MPH-induced gastrointestinal reactions may correlate closely with reduced P. distasonis . Further experiments confirmed that P. distasonis supplementation effectively alleviated diarrhea and colonic inflammatory responses in MPH-treated rats. Conclusions Collectively, this is the first study verifying that P. distasonis supplementation mitigates MPH-induced gastrointestinal adverse reactions, providing a novel intervention strategy to improve tolerance of ADHD pharmacotherapy.
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
·
IF 4.122
Shang Xiao-Fei,Liu Ying-Qian,Guo Xiao,Miao Xiao-Lou,Chen Cheng,Zhang Jun-Xiang,Xu Xiao-Shan,Yang Guan-Zhou,Yang Cheng-Jie,Li Jun-Cai,Zhang Xiao-Shuai
As important secondary plant metabolites, naphthoquinones exhibit a wide range of biological activities. However, their potential as sustainable alternatives to synthetic acaricides has not been studied. This study for the first time investigates the acaricidal activity of naphthoquinones against Psoroptes cuniculi in vitro . Furthermore, the in vivo activity, the skin irritation effects, the cytotoxicity and the inhibitory activities against mite acetylcholinesterase (AChE) and glutathione S-transferase (GST) of the two compounds that displayed the best insecticidal activity in vitro were evaluated. Among fourteen naphthoquinones and their analogs, juglone and plumbagin were observed to possess the strongest acaricidal activities against P. cuniculi with LC 50 values of 20.53 ppm and 17.96 ppm, respectively, at 24 h. After three treatments, these two chemicals completely cured naturally infested rabbits in vivo within 15 days, and no skin irritation was found in any of the treated rabbits. Compared to plumbagin, juglone presented no or weak cytotoxicity against HL-7702 cells. Moreover, these two chemicals significantly inhibited AChE and GST activity. These results indicate that juglone has promising toxicity against P. cuniculi , is safe for both humans and animals at certain doses, and could be used as a potential alternative bio-acaricide for controlling the development of psoroptic mange in agricultural applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Wu Yuehua,Liu Zhaoyi,Zhang Hongyang,Zhou Lixiao,Liang Weijuan,Su Peng,Zeng Zhijun,Zhao Feng,Qiu Jingfu,Chen Chengzhi
Obesity is a common health problem marked by the accumulation of excess body fat beyond the range characteristics of a healthy weight, affecting millions worldwide. Although advancements have been made in the realm of probiotic therapy for obesity, studies on the combined use of probiotics for the treatment of obesity have yet to be well explored. In this study, we investigated the potential of Parabacteroides goldsteinii ( P. goldsteinii ) and Bacteroides sartorii ( B. sartorii ) combinations and their microencapsulated forms for treating obesity. We identified different probiotic strains by 16S rRNA analysis in obese and healthy mice. The activity of these probiotics was evaluated in vitro under simulated gastrointestinal conditions. In vivo, the effects of probiotics on obese mice were assessed by monitoring body weight, glucose tolerance, colon integrity, and various molecular markers. Our results suggested that P. goldsteinii and B. sartorii were shown to be less abundant in obese mice ( P < 0.05). Compared with the control group mice, the obese phenotypes of the probiotics intervention group mice were significantly improved ( P < 0.05). Further results showed that compared with the control group mice, the expression of the FTO in the liver tissue of the probiotics intervention group mice was downregulated ( P < 0.05), while the expression of the Ampk gene was upregulated ( P < 0.05), indicating that probiotic intervention had reduced the expression of obesity-related genes in mice and had alleviated their obese condition. Furthermore, Microcapsule was remarkably effective in maintaining the viability of probiotics and facilitating their persistence, leading to a significant reduction in weight gain in mice compared with controls within just one week ( P < 0.05). Taken together, our study demonstrates that the intervention using the combination of P. goldsteinii and B. sartorii is more effective in treating obesity than using either probiotic alone. The encapsulation approach further enhances the anti-obesity efficacy of the combined probiotic mixture.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2023
·
IF 5.7
Sun Hanyu,Li Pugeng,Kong Qingci,Deng Feilong,Yu Xiaolin
Zoledronic acid (ZA), one of the commonly used bisphosphonates, is mainly used for bone-metabolic diseases. Studies proved that ZA has adverse effects on oral soft tissues. As the first line of innate immunity, the gingival epithelium could be infected by periodontal pathogens, which is a key process of the initiation of periodontal diseases. Yet, how ZA affects the periodontal pathogens infecting the epithelial barrier remains unclear. This study aimed to investigate the influences of ZA on the process of Porphyromonas gingivalis (P. gingivalis) infecting the gingival epithelial barrier via in-vitro and in-vivo experiments. In the in-vitro experiments, under the condition of different concentrations of ZA (0, 1, 10, and 100 μM), P. gingivalis was used to infect human gingival epithelial cells (HGECs). The infections were detected by transmission electron microscope and confocal laser scanning microscope. Besides, the internalization assay was applied to quantify the P. gingivalis, which infected the HGECs, in the different groups. To evaluate the expression levels of pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and IL-8, by infected HGECs, real-time quantitative reverse transcription-polymerase chain reactions were applied. In the in-vivo experiments, rats were given ZA solution (ZA group) or saline (control group) by tail intravenous injection for 8 weeks. Subsequently, we put ligatures around the maxillary second molars of all the rats and inoculated P. gingivalis to the gingiva every other day from day 1 to day 13. The rats were sacrificed on days 3, 7, and 14 for micro-CT and histological analyses. The in-vitro results manifested that the quantity of P. gingivalis that had infected HGECs increased with the ZA concentrations. Pro-inflammatory cytokines expression by HGECs were significantly increased by 100 μM ZA. In the in-vivo study, compared to the control group, more P. gingivalis was detected in the superficial layer of gingival epithelium in the ZA group. Besides, ZA significantly increased the expression level of IL-1β on day 14 and IL-6 on days 7 and 14 in gingival tissues. These findings suggest that the oral epithelial tissues of patients who receive high-dose ZA treatment may be more susceptible to periodontal infections, resulting in severe inflammatory conditions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Bowen Qin,Yijun Yao,Jing Zhang,Lifeng Wang
At present, most of the research on coix seed polyphenols (CSPs) focuses on the separation, purification, structure analysis, and biological functions of specific components, and few studies have considered the overall bioavailability and the metabolites that play a role after digestion and absorption and their biological functional activities. In this study, we constructed a MKN28 and Caco-2 cell monolayer continuous transport model (MCTM) to study the bioavailability of CSPs in the digestion and absorption stages of the stomach and small intestine. Using this model, we innovatively divided CSPs into easy-to-digest and hard-to-digest polyphenols and studied their intracellular lipid-lowering function and their influence on human intestinal flora. Transwell experiments showed that ferulic acid, rutin, naringin, arbutin, and syringetin had high transmembrane transport efficiency, especially syringetin. The methylation reaction in the monolayer membrane of Caco-2 cells may be the reason for the higher transport rate of syringetin. Further experiments showed that CPL reduced TG accumulation by more than 50% during 3T3-L1 differentiation and promoted the transformation of adipocytes into brown cells (p < 0.05). Finally, in vitro fermentation experiments showed that CSP_AP can increase the abundance of Lactobacillus and Bifidobacterium of human gut microbiota at the genus level (p < 0.05).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Linlin Gao,Lin Zhang,Huan Liu,Jielun Hu
In order to increase children's daily intake of dietary fiber and protein, the effects of soluble dietary fiber (SDF) supplement including high-amylose corn starch (HACS), inulin , fructo-oligosaccharides (FOS), galactose oligosaccharides (GOS), β-glucan (BG), arabinogalactan (AG), arabinoxylan (AX), and xylo-oligosaccharides (XOS) on protein digestion of multigrain noodles and the fecal microbiota of school-age children were evaluated by in vitro digestion and fermentation in this study. The addition of SDF in multigrain noodles retained a high protein content, which is beneficial for children intake. Except HACS and inulin, the addition of other SDFs significantly increased protein hydrolysis ( p < 0.05) during gastrointestinal digestion, which will enhance the protein digestion for the children. In addition, noodles (after digestion) containing SDF could reduce fermentation pH, increase short-chain fatty acid levels, and regulate microbiota composition during in vitro fermentation for 24 h. Noodles with HACS resulted in the highest short-chain fatty acids production during fermentation. Noodles containing FOS, GOS and HACS significantly increased the abundance of Bifidobacterium , Lactobacillus and Clostridium-sensu_stricto_1 ( p < 0.05) at genus level in fecal microbiota during fermentation. Results indicated that FOS, GOS and HACS are more suitable to be added to multigrain noodles to improve protein and fiber intake for school-age children.
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2022
·Vol. 14
·期 2
·857-873
·
IF 6.317
Ke Lan,Huabing Yang,Junping Zheng,Haiming Hu,Tianxiang Zhu,Xiaojuan Zou,Baifei Hu,Hongtao Liu
Poria cocos, a widely accepted function food in China, has multiple pharmacological activities. This study aimed to investigate the therapeutic effect and molecular mechanism of Poria cocos oligosaccharides (PCOs) against dextran sodium sulfate (DSS)-induced mouse colitis. In this study, BALB/c mice were treated with 3% (w/v) DSS for seven days to establish a colitis model. The results showed that oral administration of PCOs (200 mg per kg per day) significantly reversed the changes in the physiological indices in colitis mice, including body weight, disease activity index scores (DAI), spleen index, and colon length. From the qRT-PCR assay, it was observed that PCOs suppressed the mRNA expression of pro-inflammatory cytokines, such as Tnf-α, Il-1β, and Il-6. In addition, PCOs protected the intestinal barrier from damage by promoting the expression of mucins and tight junction proteins at both mRNA and protein levels. Upon 16S rDNA sequencing, it was observed that PCO treatment partly reversed the changes in the gut microbiota of colitis mice by selectively regulating the abundance of specific bacteria. And Odoribacter, Muribaculum, Desulfovibrio, Oscillibacter, Escherichia–Shigella, and Turicibacter might be the critical bacteria in improving colitis via PCOs. Finally, using antibiotic mixtures to destroy the intestinal bacteria, we documented that PCO fermentation broth (PCO FB) instead of PCOs prevented the occurrence of colitis in gut microbiota-depleted mice. In conclusion, PCOs showed a protective effect on colitis by reversing gut microbiota dysbiosis. Our study sheds light on the potential application of PCOs as a prebiotic for treating colitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）