INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Junxi Liu,Yan Liu,Yuheng Zheng,Haoyu Wang,Jinglin Wang,Yu Zhang,Kaiping Wang
SCLP remained intact in the stomach and small intestine after oral administration. • SCLP was degraded into oligosaccharide fragments in the cecum and colon. • The intestinal metabolism of SCLP was gut microbiota-dependent. • Phocaeicola vulgatus and Bacteroides uniformis were involved in SCLP metabolism. This study aimed to investigate the intestinal metabolic characteristics and mechanisms of the pectic polysaccharide isolated from the medicinal plant Smilax china L. (SCLP). Firstly, in vitro simulated digestion confirmed that SCLP remained stable in simulated digestive fluids. Subsequently, in vivo real-time tracking of intestinal metabolism based on fluorescent labeling revealed that SCLP maintained its prototype in the small intestine and began to be degraded into fragments (Mw < 4000 Da) upon reaching the cecum and colon, where it was retained for prolonged periods. Pseudo-sterile mouse experiments indicated the mediating role of gut microbiota in SCLP metabolism. Furthermore, metagenomic sequencing suggested that SCLP increased the proportion of polysaccharide utilization loci (PULs) from Phocaeicola vulgatus and Bacteroides uniformis , elevated the gene numbers of carbohydrate-active enzymes (CAZymes) including GHs, GTs and CBMs, and activated pathways of carbohydrate metabolism. Finally, in vitro bacterial culture study verified the degradation and utilization of SCLP by Phocaeicola vulgatus and Bacteroides uniformis . In summary, this work elucidates the intestinal metabolic profile of SCLP, providing valuable insights for its further development and utilization.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Wenjia Liu,Ying Wang,Xiangyu Hao,Yangxun Wang,Xiaofan Zhang,Jinchao Zhang,Kun Ge,Guoqiang Zhou
Triple-negative breast cancer (TNBC) is associated with rapid progression and poor sensitivity to monotherapies, necessitating combination strategies to enhance tumor immunogenicity, reprogram the tumor microenvironment (TME), and convert immunologically "cold" tumors into "hot" ones. In this study, we develop a pH-responsive, pyroptosis-inducing nanoreactor, ZnS/ZIF-90:Fe/Vk3 (ZZFV), designed to elicit potent antitumor immunity. Under acidic conditions, ZZFV rapidly degrades, releasing Zn 2+ , Fe 2+ , H 2 S, and Vk3. H 2 S promotes glucose uptake and consumption while downregulating cytochrome c oxidase subunit IV (COX IV) expression, whereas Zn 2+ suppresses lactate dehydrogenase (LDH) activity, collectively disrupting glucose metabolism and exacerbating intracellular acidification. Meanwhile, NAD(P)H quinone oxidoreductase 1 (NQO1), highly expressed in tumor cells, catalyzes the conversion of Vk3 to H 2 O 2 , which is subsequently converted to highly toxic •OH through Fe 2+ -mediated Fenton reactions. Additionally, Zn 2+ and H 2 S synergistically disrupt mitochondrial function, thereby amplifying reactive oxygen species (ROS) production. Excessive ROS triggers Caspase-1-dependent pyroptosis, leading to the liberation of damage-associated molecular patterns. These events promote dendritic cell (DC) maturation, robust T-cell activation, M1 macrophage polarization, and expansion of central memory T-cell populations, thereby conferring durable immune protection. Consequently, ZZFV demonstrates potent antitumor efficacy, effectively overcoming immune tolerance in TNBC and achieving robust, sustained antitumor immunity.
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Frontiers in Pharmacology
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2022
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IF 5.988
Wang Hongkang,Ji Zhe,Feng Yanru,Yan Tianhua,Cao Yongbing,Lu Hui,Jiang Yuanying
Introduction: Extrusion of azoles from the cell, mediated by an efflux pump Cdr1, is one of the most frequently used strategies for developing azole resistance in pathogenic fungi. The efflux pump Cdr1 is predominantly localized in lipid rafts within the plasma membrane, and its localization is sensitive to changes in the composition of lipid rafts. Our previous study found that the calcineurin signal pathway is important in transferring sphingolipids from the inner to the outer membrane.Methods: We investigated multiple factors that enhance the antifungal activity of fluconazole (FLC) using minimum inhibitory concentration (MIC) assays and disk diffusion assays. We studied the mechanism of action of myriocin through qRT-PCR analysis and confocal microscopy analysis. We tested whether myriocin enhanced the antifungal activity of FLC and held therapeutic potential using a mouse infection model.Results: We found that this signal pathway has no function in the activity of Cdr1. We found that inhibiting sphingolipid biosynthesis by myriocin remarkably increased the antifungal activity of FLC with a broad antifungal spectrum and held therapeutic potential. We further found that myriocin potently enhances the antifungal activity of FLC against C. albicans by blocking membrane localization of the Cdr1 rather than repressing the expression of Cdr1. In addition, we found that myriocin enhanced the antifungal activity of FLC and held therapeutic potential.Discussion: Our study demonstrated that blocking the membrane location and inactivating Cdr1 by inhibiting sphingolipids biogenesis is beneficial for enhancing the antifungal activity of azoles against azole-resistant C. albicans due to Cdr1 activation.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Huijun Wang,Jianxun Li,Chaoyang Zhang,Baoshan Sun,Yawen Zhou,Yanyan Zhou,Haiyu Zhao
Plucking position and harvest time influence the metabolites of vine tea which are closely related to the taste quality and antioxidant activity. In this study, 75 vine tea samples produced by three plucking positions and harvested in five months were analyzed by untargeted metabolomics, and in vitro and in vivo antioxidant activities of different vine teas were evaluated. 37 compounds were tentatively identified through various methods. Compared with the high-concentration results, VIP scores of the major components in the low-concentration model were much higher and were filtered as characteristic markers. Chemometrics analysis showed that plucking position had a stronger influence on vine tea quality than harvest time. 16 identified metabolites, including flavonoids, organic acids and catechins, were found to be responsible for the recognition of the plucking position. Vine tea infusion made of young green leaves exhibited higher scavenge radical activity in vitro , and tender tip leaves exhibited higher antioxidant activities in vivo zebrafish, which may be due to the presence of a higher concentration of flavonoids in their phytochemical profile. This research is anticipated to provide useful enlightenment for the effective utilization of vine tea resources.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2022
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IF 3.119
Fang Zhang,Fuwei Pan,Hairong Wang
The study mainly reported the equilibrated solubilities of nisoldipine in aqueous solutions of n-propanol/acetonitrile + water and non-aqueous solutions of ethyl acetate + ethanol/isopropanol by dint of experimentation and model correlations. Solubility was experimented via the shake-flask method under 101.2 kPa from 278.15 to 318.15 K. The maximum of nisoldipine solubility (in mole fraction unit) was found in neat n-propanol/acetonitrile/ethyl acetate at 318.15 K, and the minimum was found in neat water/isopropanol/ethanol. The total and three-dimensional Hansen solubility parameters were employed herein to explain the solubility behavior of nisoldipine. Modeling solubility through mixture response surface, Jouyban–Acree, and Jouyban–Acree–van’t Hoff models resulted in relative average deviations of <10.3%. Quantitative research upon preferential solvation was carried out by applying the approach of inverse Kirkwood–Buff integrals for the four solution systems considered in the present study and two aqueous solutions of ethanol/isopropanol reported in the literature. The local fractions of n-propanol/acetonitrile/ethanol/isopropanol for the four aqueous solutions as well as ethyl acetate for non-aqueous solution of ethyl acetate + isopropanol were greater than that of bulk ones in n-propanol/acetonitrile/ethanol/isopropanol/ethyl acetate-rich and middle composition proportions, demonstrating that the solvation of nisoldipine was preferred by cosolvents (n-propanol/acetonitrile/ethanol/isopropanol/ethyl acetate). It is able to speculate that nisoldipine displays Lewis acid behavior in these regions with the cosolvent molecules in aqueous solution systems and with the ethyl acetate molecules or isopropanol in the ethyl acetate + isopropanol solution system.
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Polymers
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2022
·Vol. 14
·期 12
·2410
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IF 4.967
Guoliang Ma,Zhiming Zheng,Han Wang,Li Wang,Genhai Zhao,Hengfang Tang,Xiumin Ding,Peng Wang
Nutritional food supplements and pharmaceutical products produced with vitamin K2as raw materials a very promising market in the global scope. The main production method of vitamin K2is microbial fermentation, but approximately 50% of vitamin K2synthesized by the main production strainBacillus subtilis nattoexists in extracellular form, which is not easy to separate and extract. In order to solve this problem, in this study, we synthesized a novel cellulose flocculant, MCC-g-LMA, by grafting reaction using microcrystalline cellulose (MCC) and lauryl methacrylate (LMA) as monomers, and ammonium persulfate as an initiator to flocculate VK2from the fermentation supernatant. The flocculant was characterized by Fourier transform infrared spectroscopy (FTIR), elemental analysis, and scanning electron microscopy (SEM), and the grafting reaction was successful. When the flocculant dosage was 48.0 mg/L and pH was 5.0, the flocculation rate of the MCC-g-LMA on the fermentation supernatant reached 85.3%, and the enrichment rate of VK2reached 90.0%. Furthermore, we explored the flocculation mechanism of VK2by the MCC-g-LMA and speculated that the flocculation mechanism mainly included adsorption bridging, hydrophobic association and net trapping and sweep effect. In this study, the extraction method for trace high-value biological products in the fermentation supernatant was improved, which provided a method and theoretical basis for the efficient separation and purification of VK2and other terpenoids.Keywords:cellulose;flocculant;Bacillus subtilis nattofermentation supernatant;vitamin K2
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Food & Function
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2022
·Vol. 13
·期 15
·8091-8106
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IF 6.317
Yanjing Ou,Mingdong Yan,Guanglin Gao,Wenjie Wang,Qiaoqiao Lu,Jiang Chen
Cinnamaldehyde (CA), the main active ingredient in cinnamon, has been proved to be a potential candidate for controlling inflammation; however, there has been little evidence demonstrating its role in alleviating periodontitis. The aim of this study was to investigate the effect of orally administered CA on ligature-induced periodontitis in mice and the administration of CA on the Porphyromonas gingivalis (Pg) supernatant-induced inflammatory responses of murine macrophages and human periodontal ligament cells (HPDLCs). In vivo experiments showed that the oral administration of CA significantly inhibited bone resorption, the accumulation of anaerobic bacteria and host immuno-inflammatory responses in ligature-induced periodontitis in mice. In vitro, CA inhibited the expression of Pg supernatant-induced IL6, IL8, TNFA and IL1B and reactive oxygen species in RAW 264.7 and HPDLCs, involving the inactivation of the NFKB signaling pathway, which was activated by the Pg supernatant. Also, the expression of adherent and chemotactic-related cytokines was inhibited by CA, accompanied with a reduction in adherent HPDLCs. Moreover, CA ameliorated the cellular senescence of HPDLCs induced by H2O2, together with a decrease in senescence-associated-β-galactosidase positive cells and decrease in the expression of P53, P21 and P16. Furthermore, CA promoted the osteogenic differentiation of HPDLCs with an increase in alkaline phosphatase expression and activity, formation of more mineralization nodules, and increased the expression of bone sialoprotein and osteopontin. Conclusions: Daily diet-added CA may be beneficial for oral health care, especially for the control of periodontic disease by suppressing the dysbiosis of biofilms and inhibiting the immunoinflammatory responses of migrated macrophages and local resident periodontal ligament cells to specific pathogen irritations.
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Journal of Cosmetic Dermatology
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2022
·Vol. 21
·期 11
·6145-6155
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IF 2.189
Jiahong Guo,Xiaoyu Ma,Fernando Bouffard,Sophia Yi Zhang
Background Fruit acids have long been recognized as highly effective actives with world-wide popularity, covering skin peeling, anti-acne, anti-wrinkle applications, and skin depigmentation. Aims There are complicated interconnections between the fruit acid formula and skin pigmentation behaviors. However, the lack of systematic researches on multi-ingredient formula restricted our understanding on its mechanism. Therefore, it is of great necessity to study the interactions and cascades among components, potential gene targets, signaling pathways, and biological processes via a system biology approach. Methods We used system biology, molecular fingerprint, and structural biology to calculate and collect target information of the functional formula. Gene Ontology (GO) enrichment analysis was further applied to study the biological processes and pathways of the formula, and a multi-level network model of “component - molecular target - signaling pathway - skin disease” was established. Besides, the zebrafish model was utilized to verify the formula. Results We obtained 69 hub targets by constructing a protein–protein interaction (PPI) network based on the intersection between multi-fruit acids formula (mandelic acid, lactobionic acid, niacinamide, and hydroxytyrosol) and skin indications targets (whitening-sebum balance). In vitro zebrafish models, including pigmentation, antioxidant, and radiation protection models, showed that the current formula significantly alleviated pigmentation intensity and intracellular free radical content, thus proving the efficacy of skin brightening and UV irradiation protection. Conclusions This research uncovered the underlying mechanism of multi-fruit acids formula and predicted its function in skin brightening and UV irradiation prevention.
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DRUG DELIVERY
·
2021
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IF 6.419
Bingfeng Liang,Dangxia Zhou
Although podophyllotoxin (POD) demonstrates high efficiency to inhibit various cancers, its clinic application is limited to poor bioavailability. Nanoparticles derived from homodimeric prodrugs with high drug loading potential are emerging as promising nanomedicines. However, complete intracellular drug release remains a major hindrance to the use of homodimeric prodrugs-based nanomedicine. We sought to develop a reactive oxygen species (ROS) responsive POD dimeric prodrug by incorporating vitamin K3 (VK3) and Pluronic F127 to synthesize a spheroid nanoparticle (PTV-NPs). PTV-NPs with high POD content could release drugs under the ROS enrichment microenvironment in cancer cells. The released VK3 could produce abundant ROS selectively in tumor cells catalyzed by the overexpressed NAD(P)H: quinone oxidoreductase-1 (NQO1) enzyme. In turn, the resultant high ROS concentration promoted the conversion of POD dimeric prodrug to POD monomer, thereby achieving the selective killing of cancer cells with weak system toxicity. In vitro and in vivo studies consistently confirmed that PTV-NPs exhibit high drug loading potential and upstanding bioavailability. They are also effectively internalized by tumor cells, induce abundant intracellular ROS generation, and have high tumor-specific cytotoxicity. This ROS-responsive dimeric prodrug nanoplatform characterized by selective self-amplification drug release may hold promise in the field of antitumor drug delivery.
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Bioactive Materials
·
2021
·
IF 14.593
Bailiang Wang,Liyang Zhou,Yishun Guo,Hanwen Guo,Yiming Zhong,Xiaomin Huang,Yifan Ge,Qingying Wang,Xiaoying Chu,Yingying Jin,Kaiyue Lan,Mei Yang,Jia Qu
Photodynamic therapy (PDT) is an important technique to deal with drug-resistant bacterial infections in the post-antibiotic era. However, the hypoxic environment in intractable infections such as refractory keratitis and periodontitis, makes PDT more difficult. In this work, spontaneous oxygen-producing cyanobacteria were used as the carrier of photosensitizer (Ce6), and ultrasmall Cu 5.4 O nanoparticles (Cu 5.4 O USNPs) with catalase activity for infection and inflammation elimination and rapid tissue repair (CeCycn-Cu 5.4 O). The loading of Ce6 and Cu 5.4 O USNPs onto cyanobacteria surface were confirmed by transmission electron microscopy, nano particle size analyzer, scanning electron microscopy. In vitro sterilization and biofilm removal experiments demonstrated that the restriction of hypoxic environment to PDT was significantly alleviated due to the oxygen production of cyanobacteria. Under laser irradiation, the close transfer of energy photons to oxygen produced by cyanobacteria reduced more than 90% of Ce6 dosages (660 nm, 200 mW/cm 2 , 2 min). It is worth mentioning that both rapid sterilization through PDT and long-term oxidized free radicals elimination were achieved by adjusting the ratio of Ce6 and Cu 5.4 O USNPs. Both periodontitis and refractory keratitis animal models proved the excellent self-oxygenation enhanced antibacterial property and promotion of tissue repair.
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Nanoscale
·
2021
·Vol. 13
·期 47
·20078-20090
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IF 7.79
Kai Zhang,Zhonghui Zhuo,Guorong Fan,Zongde Wang,Shangxing Chen,Lulu Xu,Yangping Wen,Peng Wang
The simultaneous detection of multiple biological small molecules is critical for human health evaluation and disease prevention. In this study, a nano-ZnS decorated hierarchically porous carbon (ZSHPC) electrocatalyst with multiple enzyme-like activities as a nanozyme sensing platform for simultaneous derivative voltametric detection of four important biological small molecules, dopamine (DA), uric acid (UA), guanine (G), and adenine (A), is successfully synthesized via an in situ hydrothermal reaction using leaves of Cinnamomum camphora (L.) after the extraction of essential oil as a carbon source, ZnCl2 as both zinc source and an activator, sulfuric acid as a sulfur source, and silica gel as a hard template. Activator together with the introduction of silica gel is beneficial for tuning pore structure. The in situ synthesized ZnS nanoparticles and sulfur doping improve the conductivity and cycling stability of the material. The ZSHPC electrode with multiple enzyme-like activities and oxidase-like characteristics was employed for the simultaneous detection of multiple target molecules in linear ranges of 0.3–500 μM with detection limits of 0.12 μM for DA, 0.26 μM for UA, 0.07 μM for G, and 0.075 μM for A. A derivative technique was selected for enhancing the peak resolution of the partial overlapped voltammograms and eliminating human error. Both the coefficient of determination and residual prediction deviation were used to evaluate this technique.
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FIBERS AND POLYMERS
·
2021
·Vol. 22
·期 5
·1208-1217
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IF 2.153
Zhao Chenyu,Wang Guangshu,Sun Mengtao,Cai Zewei,Yin Zichu,Cai Yurong
As one of the most important parameters to characterize the organic pollution of water, biochemical oxygen demand (BOD) determined through a rapid, in situ and on line method is very attractive. In this paper, a new BOD biosensor has been developed, which are composed of the ecofriendly precursor of Saccharomyces cerevisiae ( S. cerevisiae ) embedded in bacterial cellulose (BC) matrix and the Ketjen Black (KB) modified glassy-carbon electrode. Furthermore, a doublemediator system is constructed using potassium ferricyanide and menadione in reaction cell to transfer electron from the microbe to electrode. The responses of glucose glutamic acid (GGA) standard solutions are amperomertrically measured with an applied potential of 0.25 V versus Hg/Hg 2 Cl 2 in a three-electrode system. Under the optimum conditions, the (BC/ S. cerevisiae -menadione)/KB modified electrode shows a high operational stability with relative standard deviation (RSD) of 4.16% (fourteen assays), a good repeatability (RSD=3.10%), a fast response time (in 20 minutes) and a wide linear range (from 10–220 mg O 2 l −1 ). The BOD values measured by this method have been highly correlated with the standard BOD 5-day method for wastewater samples (R 2 =0.9859, n=3), indicating that it can meet the requirement of BOD rapid measurement.
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