JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2016
·
IF 3.782
Daqun Chen,Lei Zhao,Weihua Hu
Mussel inspired polydopamine (PDA) film has attracted great interest as a versatile functional coating for biomolecule immobilization in various bio-related devices. However, the details regarding the interaction between a protein and PDA film remain unclear. Particularly, there is very limited knowledge regarding the protein immobilization on PDA film, even though it is of essential importance in various fields. The situation is even more complicated if considering the fact that quite a number of approaches (e.g., different oxidizing reagent, buffer pH, grown time, grown media, etc.) have been developed to grow PDA films. In this work, protein attachment on PDA film was systematically investigated by using the real-time and label-free surface plasmon resonance (SPR) technique. The kinetics of protein-PDA interaction was explored and the influence of buffer pH and deposition media on the protein attachment was studied. Fluorescent protein microarray was further printed on PDA-coated glass slides for quantitative investigations and together with SPR data, the interesting fluorescence quenching phenomenon of PDA film was revealed. This work may deepen our understanding on the PDA-protein interaction and offer a valuable guide for efficient protein attachment on PDA film in various bio-related applications.
研究领域:
生物技术
·
生物材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Separation Science Plus
·
2019
·Vol. 2
·期 5
·178-185
·
IF 0
Zhifeng Huang,Yingxiang Du,Xiaoqi Li,Xiaodong Sun,Zijie Feng,Xiaofei Ma
Transfersomes, a special kind of phospholipid vesicles with higher deformability and flexibility, were applied as coating material to prepare a transfersomes-coated capillary column. A capillary electrochromatography enantioseparation system based on the new transfersomes-coated column with carboxymethyl-β-cyclodextrin added to the running buffer as a chiral selector was established for the first time. Compared with a bare column, three basic drugs achieved baseline separation with better resolution (3.17 for Propranolol, 2.38 for Metoprolol, 2.34 for Esmolol) in this transfersomes-electrochromatography system. Several important parameters including concentration of chiral selector, buffer pH and applied voltage were systematically investigated to provide the optimal separation condition. This study constructed a flexible and efficient enantioseparation electrochromatography system with a small amount of transfersomes, and showed that transfersomes as the stationary phase could efficiently improve chiral separation effect.
研究领域:
分析化学
·
药学
·
色谱学
✓ 已验证
产品关联
DOI
Journal of Food Measurement and Characterization
·
2023
·
IF 3.4
Du Huan,Li Tao,Xue Qiaoli,Tian Yang,Hu Yongjin
Moringa oleifera leaves are potentially benign sources of natural folates, thus a reliable folate extraction protocol is necessary, which directly affects the accuracy of subsequent determination of total folates or various folate derivatives. With this aim, process variables including antioxidants, extraction buffer pH, Moringa oleifera leaves powder (MOLP)/extraction buffer ratio, heating extraction time, amounts of α- amylase, protease and rat serum, and also incubation time of rat serum were investigated and optimized by response surface methodology (RSM). It is interesting that no positive influence of α- amylase or protease solution was observed in this study, consequently a single-enzyme treatment was used. An average folate level of 1190.06 µg/100 g dry matter was achieved by microbial assay under the optimized conditions of extraction buffer pH 7.0, MOLP/extraction buffer ratio 1:24 (g/mL), rat serum 350 µL, digest time 2.0 h, in good agreement with the value predicted by quadratic model. In addition, the validity of protocol established in this study was confirmed. The intra-day precision (relative standard deviation) varied between 1.42% with 1.84%, inter-day precision varied in the range of 1.34–2.25%. The average recoveries were in the range of 101.56–103.27%. Besides, the matrix effects ranged from 101.41 to 103.34%. The protocol could provide a reference to fairly evaluate folates in Moringa oleifera leaves from diverse sources, and even in other leafy plants. Meanwhile, Moringa oleifera leaves with high folate level seem to have tremendous potential to increase nutritional value of foods as well as to enhance regional economies.
研究领域:
食品科学
·
营养学
·
分析化学
·
植物科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-8
·
IF 6.232
Sun Xiaodong,Chen Cheng,Li Xiaoqi,Du Yingxiang,Zhao Shiyuan,Feng Zijie
A new bilayer chiral stationary phase for use in open tubular capillary electrochromatography (OT-CEC) system is described. Gold nanoparticles were modified with L-cysteine, with a tetramethylammonium lactobionate ionic liquid that acts as the chiral selector. The gold nanoparticle-coated column provides good enantioseparation and favorable reproducibility. Compared with an uncoated separation system, the column developed displays improved separation of the racemic β-blockers propranolol, esmolol, bisoprolol and sotalol (resolutions of enotiomers are 6.29, 6.11, 6.12 and 6.02, respectively). The materials and coatings were characterized by scanning electron microscopy and transmission electron microscopy. The main driving forces (CEC and electro-osmotic flow) were studied to evaluate the variation of the immobilized columns. The effects of buffer pH value, concentration of chiral selector, type of organic modifier and applied voltage were optimized. Satisfactory relative standard deviations were achieved in run-to-run, day-to-day and column-to-column experiments.
Schematic preparation of a capillary column with bilayer chiral selectors coated gold nanoparticles. This novel OT-CEC system was applied for separation of four basic racemic β-blockers.
研究领域:
分析化学
·
药学
·
纳米技术
·
色谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）