TALANTA
·
2025
·
IF 6.1
Chen Yang,Guoqing He,Shukun Zhao,Shanpeng Dong,Yantong Liu,Yifan Wang,Tao Jiang,Yihan Ma,Longfei Chen,Xiaotong Si,Shilun Feng,Xuan Xiao,Yi Yang
Real-time detection of chloramphenicol (CAP) in food can effectively safeguard food safety. However, traditional chloramphenicol detection methods require large testing equipment, specialized operators and long testing times, therefore portable and rapid CAP detection remain a challenge. Here, we present a differential encodable microspheres separation system based (DEMSs) rapid, portable and accurate detection of CAP. DEMSs utilize specific binding of CAP-Antibody in DEMSs to antigen (CAP) in sample and filtration separation using a customized microfluidic chip for probe-free and wash-free CAP immunoassays. Integrated microscopic optical system and smart counting algorithms based on Hough Circle Transform deployed on a smartphone app enable portable and on-site CAP detection. The experimental results showed that the limit of detection (LoD) of CAP is 24.3 pg/mL and the detection range is 30-50,000 pg/mL. The single testing time is only 16 min. The device offers a novel CAP immunoassay strategy. By replacing antibodies coupled to encoded microspheres in DEMSs, this system is expected to enable the detection of multiple substances in foods.
研究领域:
食品安全
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2016
·Vol. 26
·期 34
·6146-6157
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IF 11.382
Ding Sheng-zi Zhang,Yang Jiang,Haiou Yang,Youjie Zhu,Shunjia Zhang,Ying Zhu,Dan Wei,Ye Lin,Pingping Wang,Qihua Fu,Hong Xu,Hongchen Gu
Bead-based suspension arrays have attracted increasing attention because of the fast growing requirements for high-throughput multiplexed bioassays. Here, novel dual-encoded microbeads (DEBs) based on a host–guest structure are developed. A strawberry-like structure composed of thousands of dye-doped nanoparticles closely packed onto the quantum dot-encoded microbeads is designed and synthesized. Using this 3D structure, the encoded space is fully developed for multicolor encoding. As a result, a 30-barcode library with an outstanding encoding capacity is successfully established and easily decoded via flow cytometry. The separation of organic dyes and quantum dots into two building blocks helps circumvent the optical mutual interference commonly observed in most multicolor barcodes, bringing great convenience for next-step decoding accuracy. As a proof of concept, six typical DEBs are chosen and their feasibility in six-plexed detection for influenza viruses is evaluated. The results show that DEBs can selectively capture the specific targets, with negligible nonspecific absorption; these results thus demonstrate the strong potential of the DEBs as a robust platform for multiplexed bioassays.
研究领域:
生物技术
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分析化学
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医学检测
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 32
·11110-11118
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IF 7.059
Dong Mo,Zhengqiang Wang,Kaiyao Sun,Xiyue Xie,Jixi Zhang,Kaiyong Cai
The photoluminescence stability of lanthanide complexes (Lnx) in biological media is of the utmost significance for the construction of optically encoded nano-on-microbeads in multiplex bioassays. Herein, we propose a new type of encoding guest consisting of MOF@MOF nanohybrids, where isomorphic ZIF-8 and ZIF-90 were employed as the core and shell domain, respectively, to separately modulate the hosting and the protection of the inner Lnx guests. Especially, the thin ZIF-90 shell epitaxially grown on the ZIF-8 core was employed as an intermediate layer to facilitate surface covalent fluorination via an aldehyde ligand. Thereby, a superhydrophobic surface was obtained, which led to strong water-resistance of the hybrid nanoparticles, stable host–guest structure, and high quantum yields (19.1%) in biological media. A coating layer of a surface active polymer (polydopamine, PDA) was utilized to conjugate the MOF nanoparticles on polystyrene microspheres for building the second-level host–guest architecture of 3D core–satellite pluralistic hybrids. Finally, single/dual-color encoded beads (EBs) were obtained by altering the ratios of the hybrid MOF guests emitting at different wavelengths. As a proof of concept, the detection of a model tumor marker (alpha-fetoprotein, AFP) was successfully performed using the encoded beads. These findings can pave the way for the rational design of core–shell MOFs and hierarchical structures with high synergy and excellent flexibility toward stable photoluminescence of lanthanide complexes in bioassays.
研究领域:
材料科学
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生物医学工程
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分析化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yixi Liu,Le Liu,Yonghong He,Qinghua He,Hui Ma
Quantum dots encoded microbeads have various advantages such as large surface area, superb optical properties and the ability of multiplexing. Molecularly imprinted polymer that can mimic the natural recognition entities has high affinity and selectivity for the specific analyte. Here, the concept of utilizing the quantum dots encoded microbeads as the supporting material and the polydopamine as the functional monomer to form the core–shell molecular imprinted polymer was proposed for the first time. The resulted imprinted polymer can provide various merits: polymerization can complete in aqueous environment ; fabrication procedure is facile and universal; the obvious economic advantage; the thickness of the imprinting layer is highly controllable; polydopamine coating can improve the biocompatibility of the quantum dot encoded microbeads. The rabbit IgG binding and flow cytometer experiment result showed the distinct advantages of this strategy: cost-saving, facile and fast preparation procedure. Most importantly, the ability for the multichannel detection, which makes the imprinted polydopamine modified encoded-beads very attractive in protein pre-concentration, recognition, separation and biosensing.
研究领域:
生物化学
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分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Wenjing Lei,Liangwen Hao,Han Qiu,Kexin Bian,Tianming Cui,Weiwei Zeng,Yu Zhang,Weitao Yang,Bingbo Zhang
Nucleic acid detection plays a pivotal role in the accurate diagnosis of diseases. The CRISPR/Cas detection system, noted for its significant utility in a variety of applications, often necessitates enhanced sensitivity or specific signal amplification strategies, particularly for detecting low-abundance biomarkers. In this study, we present a quantum-dot-encoded beads (QDB)-energized CRISPR/Cas12-based lateral-flow assay (QDB-CRISPR-LFA). This method enables amplification-free, sensitive, and rapid detection (<40 min) of BRCA-1. We validated our method using contrived reference samples and nucleic acids extracted from tumor cells. The QDB-CRISPR-LFA provides a visual, more rapid alternative to the traditional BRCA-1 real-time RT-PCR assay. Significantly, through the integration of CRISPR’s specificity and the high signal output of QDB, the detection threshold for BRCA-1 has been reduced to the femtomolar level, representing an enhancement of 2–4 orders of magnitude over existing CRISPR/Cas detection methods. This advancement underscores the potential of our approach in advancing nucleic acid detection techniques, which is crucial for the early and precise diagnosis of diseases.
研究领域:
分子生物学
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生物技术
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医学诊断
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遗传学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Lijuan Sun,Yichen Zhang,Zhinan Fan,Chi Zhang,Li-Juan Fan
Fluorescence-encoded microspheres are largely demanded in high-throughput assays. A strategy for preparing encoded microspheres based on fluorescence resonance energy transfer (FRET) was advanced. Two fluorophores, poly(phenylenevinylene) nanoparticles (PPV NPs) as the donor and rhodamine 6G (R6G) as the acceptor were employed to construct the energy-transfer pair, and introduced onto the substrate polymeric microspheres. The fluorescence intensity ratio between PPV NPs and R6G molecules were tuned based on energy transfer mechanism. Nine codes (wavelength-intensity combinations) were obtained at the same excitation by keeping the concentration of PPV NPs constant and varying the concentration of R6G in the dipping solutions. Then, a melamine–formaldehyde (MF) resin layer was coated onto the encoded spheres to prevent the leakage of fluorophores. The encoded spheres were found to have good fluorescence stability against various solvents and even “dissociation agent“, and no significant variation of the codes were observed. Further modification of this MF layer with silanization reaction provided the amino reactive sites. In all, this study provides a general strategy for the preparation of fluorescence encoded microspheres based on energy transfer as well as a stable protective layer with reactive sites.
研究领域:
生物化学
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材料科学
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分析化学
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DOI
高影响因子
Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 31
·8262-8271
·
IF 4.696
Yan Li,Youshen Wu,Cheng Luo,Bo Wang,Daocheng Wu
Rewritable magnetic fluorescence-encoded microspheres were prepared by coating magnetic microspheres with a fluorescence-encoded melamine-formaldehyde (MF) shell. Fluorescence encoding was realized by varying the dye types and concentrations incorporated into the MF shell. Ten sets of magnetic fluorescence-encoded microspheres including four sets doped with single dye and six sets doped with two dyes were obtained and successfully identified through flow cytometric analysis. High encoding capacity could be achieved by increasing the dye number and intensity levels. More importantly, the original fluorescence-encoded MF shell could be removed via calcination. The inner magnetic microspheres remained unaffected and could be re-coated with a new fluorescence-encoded MF shell easily. The fluorescent MF coating (encoding writing) and calcination (encoding erasing) composed a cycle. Results demonstrated that the magnetic microspheres retained their original characteristics after writing–erasing cycles and the writing–erasing cycle could be repeated numerous times. These kinds of rewritable magnetic fluorescence-encoded microspheres enabled recycling and regeneration. Combining magnetic separation and re-encoding, which can significantly simplify the analysis processes and decrease the analysis cost, these microspheres are expected to be widely used in suspension arrays for high-throughput analyses.
研究领域:
材料科学
·
化学
·
生物传感
·
分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Min Hou,Liyang Shi,Yancen Zhou,Jiao Wang,Jiali Jiang,Jianhui Jiang,Jianjun He
Although suspension array technology (SAT), which uses encoded microspheres , provides high-quality results with versatile applicability for information-intensive bioanalytic applications, current encoding strategies limit the number of codes that can be distinguished. In this paper, we introduce density-encoded hydrogel microcarriers (DMs), which employ the intrinsic density property of biomaterials as a high-capacity coding dimension. Two hydrogel monomers were employed at different ratios to synthesize microgels with distinctive densities. DMs not only can be simultaneously decoded and separated using density gradient centrifugation, but also are compatible with flow cytometry detection. The size and color of DMs have been used as extra coding parameters, to construct an 8 × 2 × 4 (density × size × color) three-dimensionally encoded hydrogel microcarrier library. With aptamer-functionalized DMs (ADMs), we developed a 4-plex protein quantification method for the label-free detection of plasma biomarkers with sub-nanomolar detection limits and good linearities. Moreover, ADMs can be used for label-free naked-eye detection of tumor-derived exosomes. We believe that the simplicity and functionality of DMs will advance the field of suspension arrays and inspire the development of DM-based diagnostic applications.
研究领域:
生物技术
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生物分析化学
·
诊断医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）