ADVANCED FUNCTIONAL MATERIALS
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2016
·Vol. 26
·期 34
·6146-6157
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IF 11.382
Ding Sheng-zi Zhang,Yang Jiang,Haiou Yang,Youjie Zhu,Shunjia Zhang,Ying Zhu,Dan Wei,Ye Lin,Pingping Wang,Qihua Fu,Hong Xu,Hongchen Gu
Bead-based suspension arrays have attracted increasing attention because of the fast growing requirements for high-throughput multiplexed bioassays. Here, novel dual-encoded microbeads (DEBs) based on a host–guest structure are developed. A strawberry-like structure composed of thousands of dye-doped nanoparticles closely packed onto the quantum dot-encoded microbeads is designed and synthesized. Using this 3D structure, the encoded space is fully developed for multicolor encoding. As a result, a 30-barcode library with an outstanding encoding capacity is successfully established and easily decoded via flow cytometry. The separation of organic dyes and quantum dots into two building blocks helps circumvent the optical mutual interference commonly observed in most multicolor barcodes, bringing great convenience for next-step decoding accuracy. As a proof of concept, six typical DEBs are chosen and their feasibility in six-plexed detection for influenza viruses is evaluated. The results show that DEBs can selectively capture the specific targets, with negligible nonspecific absorption; these results thus demonstrate the strong potential of the DEBs as a robust platform for multiplexed bioassays.
研究领域:
生物技术
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分析化学
·
医学检测
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产品关联
DOI
高影响因子
Advanced Optical Materials
·
2025
·
IF 7.2
Yun Liu,Junwen Yu,Quanwang Niu,Xiangfu Wang
Optical information encryption is an effective security strategy currently used to protect data and prevent information leakage. However, the optical information encryption technology based on mechanoluminescent (ML) materials faces challenges of the single encryption mode and the lack of systematic dual-key decryption strategies. In this study, a three-layer structure optical encoder is proposed using porous ML material as the luminescent layer, a quartz transparent tube with pressure-sensing capabilities as the stress-loading layer, and photodetector as the optical recognition layer. A dual-key encryption system based on the evolution mechanism of the Ba-Gua is constructed. The encoding process of the optical encoder is completed within a micro darkroom fabricated using 3D printing technology. This system incorporates virtual position encoding in conjunction with trigram orientation, requiring two keys for correct decryption: the “Combination Relative Optical Intensity Ratio” and the “Virtual Position Space Code”. By linking the ML emission spectrum with the trigram information, a dynamic, rewritable encryption system for hybrid encoding of digital and Chinese character based on Ba-Gua evolution is established. The proposed optical encoder and dual-key encryption system offer new directions for concealing confidential information and multidimensional encryption applications.
研究领域:
光学
·
信息安全
·
材料科学
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DOI
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Zongguang Tai,Jinyuan Ma,Jianing Ding,Huijun Pan,Rongrong Chai,Congcong Zhu,Zhen Cui,Zhongjian Chen,Quangang Zhu
Purpose The clinical management of patients with castration-resistant prostate cancer (CRPC) is difficult. However, novel treatment methods are gradually being introduced. Considering the adverse effects of traditional treatments, recent studies have investigated gene therapy as a method to combat CRPC; but, the application of long non-coding (lnc) RNA in gene therapy remains scarce, despite their promise. Therefore, it is imperative to develop a system that can efficiently deliver lncRNA for the treatment of CRPC. Here, we investigated the efficacy of a delivery system by introducing the plasmid-encoding tumor suppressor lncRNA MEG3 (pMEG3) in CRPC cells.Materials and Methods An EpDT3 aptamer-linked poly(amidoamine) (PAMAM) dendrimer targeting EpCAM was used to deliver pMEG3 in CRPC cells. The PAMAM-PEG-EpDT3/pMEG3 nanoparticles (NPs) were tested using in vitro cellular assays including cellular uptake, entry, and CCK-8 measurement, and tumor growth inhibition, histological assessment, and safety evaluations in in vivo animal models.Results The EpDT3 aptamer promoted endocytosis of PAMAM and PAMAM-PEG-EpDT3/pMEG3 NPs in CRPC cells. PAMAM-PEG-EpDT3/pMEG3 NPs exhibited a significant anti-CRPC effect, both in vivo and in vitro, when compared to that of unfunctionalized PAMAM-PEG/pMEG3 NPs.Conclusion PAMAM-PEG-EpDT3/pMEG3 NPs can potentially improve gene therapy in CRPC cells.
研究领域:
肿瘤学
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分子生物学
·
药学
·
基因治疗
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产品关联
DOI
PubMed
高影响因子
CURRENT BIOLOGY
·
2025
·
IF 7.5
Junyu Zhao,Baishun An,Ning Cheng,Qiqi Dong,Cheng Wang,Xiaojing Chen
Reference frame is an essential concept for spatial navigation. The principles underlying the choice of reference frames by neuronal ensembles, especially under natural conditions, which involve sloping terrains, remain unclear. The posterior parietal cortex (PPC) is a central region for navigation and movement control, and it contains several distinct types of reference frames. Here, we investigated the influence of gravity signals on egocentric tuning and allocentric head direction tuning in the mouse PPC by introducing a mismatch between visual input and gravitational signals by tilting the ground. We found that the allocentric head direction tuning and egocentric boundary bearing tuning were differentially controlled by altered gravity signals. Specifically, the allocentric head direction signals became stronger, whereas the egocentric boundary signal was weakened. However, across horizontal and tilted conditions, the head direction tuning was less consistent, as the head directional signal could be anchored to a visual or gravity frame of reference. As the mismatch between gravitational and visual inputs increased, the gravity-anchored head direction cells became dominant. Notably, individual head direction cells can switch between reference frames. Together, these results suggest that PPC neurons exhibit a highly flexible selection of reference frames based on task demands, which may allow efficient navigation strategies in natural environments.
研究领域:
神经科学
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认知神经生物学
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空间行为学
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感觉整合
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运动控制
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xuejing Chen,Qinghua He,Tian Guan,Yonghong He,Guangxia Feng,Bei Wang,Bangrong Lu,Yanhong Ji
Aiming to satisfy the growing requirements of high-throughput multiplexed bioassays, a dual-digital encoded suspension array (SA) analysis based on Raman spectroscopy and laser induced breakdown spectroscopy (LIBS) is proposed. The SAs are prepared by the core-shell microbeads with Raman reporter molecules doped inside and elementary encoded nanoparticles (EENPs) assembled on the surface. Raman scattering spectra and LIBS spectra are obtained after excitation by laser, the specific peaks from the spectra are used for decoding. Highly sensitive and selective recognition of dual-digital encoded SA are realized by immunoassay experiments with a detection limit of 9.17 pM. Owing to the narrow bandwidths and unchangeable read-out wavelengths, the above two types of spectra provide high accuracy and stability for encoding. Meanwhile, Raman and LIBS spectra are independent from fluorescence, thus the cross talk between the encoding signal and fluorescent label on analytes can be avoided. Moreover, since the coding number grows exponentially along with the increase of types of encoding materials, the combination of two channels promote a considerable rise in detection throughput. All the above properties demonstrate the unique potential of dual-digital encoded SA in high-throughput biological detection.
研究领域:
生物技术
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分析化学
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生物传感器
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光谱学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2018
·
IF 5.123
Bangrong Lu,Qinghua He,Yonghong He,Xuejing Chen,Guangxia Feng,Siyu Liu,Yanhong Ji
To achieve the dual-channel (analog and digital) encoding, microbeads assembled with quantum dots (QDs) and element coding nanoparticles (ECNPs) have been prepared. Dual-spectra, including fluorescence generated from quantum dots (QDs) and laser induced breakdown spectrum obtained from the plasma of ECNPs, including AgO, MgO and ZnO nanoparticles, has been adopted to provide more encoding amounts and more accurate dual recognition for encoded microbeads in multiplexed utilization. The experimental results demonstrate that the single microbead can be decoded in two optical channels. Multiplexed analysis and contrast adsorption experiment of anti-IgG verified the availability and specificity of dual-channel-coded microbeads in bioanalysis . In gradient detection of anti-IgG, we obtained the linear concentration response to target biomolecules from 3.125 × 10 −10 M to 1 × 10 −8 M, and the limit of detection was calculated to be 2.91 × 10 −11 M.
研究领域:
生物分子检测
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纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yawen Li,Xuefan Qiu,Chencan Li,Zhihan Sun,Wei Qiu,Ling Wang
The dynamic control of fluorescence intensity in a time-dependent manner is crucial for enhancing the complexity of dynamic information encryption. In this study, we introduce a new system that combines a pH-responsive microgel with a non-equilibrium Eu-containing polyoxometalate. This system is created through dissipative self-assembly based on urease, enabling precise regulation of fluorescence intensity on a time scale. This intelligent fluorescent system exhibits potential applications in fluorescent ink, enzymatic logic gate and information encryption. Moreover, by simply manipulating the concentration of urease, the lifetime of encoded information can be accurately regulated. These time-encoding characteristics endow Eu-containing polyoxometalate with significant potential for advanced information encryption and data storage.
研究领域:
材料科学
·
生物化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 31
·8262-8271
·
IF 4.696
Yan Li,Youshen Wu,Cheng Luo,Bo Wang,Daocheng Wu
Rewritable magnetic fluorescence-encoded microspheres were prepared by coating magnetic microspheres with a fluorescence-encoded melamine-formaldehyde (MF) shell. Fluorescence encoding was realized by varying the dye types and concentrations incorporated into the MF shell. Ten sets of magnetic fluorescence-encoded microspheres including four sets doped with single dye and six sets doped with two dyes were obtained and successfully identified through flow cytometric analysis. High encoding capacity could be achieved by increasing the dye number and intensity levels. More importantly, the original fluorescence-encoded MF shell could be removed via calcination. The inner magnetic microspheres remained unaffected and could be re-coated with a new fluorescence-encoded MF shell easily. The fluorescent MF coating (encoding writing) and calcination (encoding erasing) composed a cycle. Results demonstrated that the magnetic microspheres retained their original characteristics after writing–erasing cycles and the writing–erasing cycle could be repeated numerous times. These kinds of rewritable magnetic fluorescence-encoded microspheres enabled recycling and regeneration. Combining magnetic separation and re-encoding, which can significantly simplify the analysis processes and decrease the analysis cost, these microspheres are expected to be widely used in suspension arrays for high-throughput analyses.
研究领域:
材料科学
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化学
·
生物传感
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分析化学
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Xiaobin Ren,Zhirong Liu,Song Wang,Zelin Cao,Kaikai Gao,Guangdong Zhou,Lu Ye,Bai Sun
Despite the progress in machine vision, existing image recognition systems still operate in isolation from the physical world, lacking dynamic temporal encoding, in-memory processing, and intrinsic interpretability. Here, a memristive vision architecture that integrates multimodal sensing, physical memory, and medical inference within a unified device-level framework is developed. Specifically, spatial gradients from retinal fundus images are encoded into Ag/Indigo/FTO memristor array via pulse modulation, generating conductance maps (“G maps”) that serve both as nonvolatile memory and as neuromorphic inputs for lesion segmentation via lightweight U-Net models. Beyond static vision, the same memristor exhibits strong temporal sensitivity, enabling biologically realistic nociceptive encoding and Morse code recognition. These results demonstrate a cross-task, cross-scale system that merges physical encoding with machine intelligence, bridging the gap between material dynamics and high-level perception. Therefore, this paradigm offers a scalable, interpretable hardware foundation for next-generation bio-perceptive computing and intelligent diagnostics.
研究领域:
神经形态工程
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生物医学成像
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人工智能
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非易失性存储器
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可穿戴传感器
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医学诊断
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产品关联
DOI
高影响因子
DEVELOPMENTAL CELL
·
2025
·
IF 8.7
Meng Wang,Kailin Liu,Wu Bao,Bingqing Hang,Xianjiong Chen,Xinyi Zhu,Guifang Li,Lihong Liu,Haoyi Xiang,Hai Hu,Yanhui Lu,Zhangfa Song,Jiaxin Chen,Yuhao Wang
The intestinal microbiota is a key environmental factor in the development of colorectal cancer (CRC). Here, we report that, in the context of mild colonic inflammation, the microbiota protects against colorectal tumorigenesis in mice. This protection is achieved by microbial suppression of the long non-coding RNA (lncRNA) Snhg9 . Snhg9 promotes tumor growth through inhibition of the tumor suppressor p53. Snhg9 suppresses p53 activity by dissociating the p53 deacetylase sirtuin 1 (SIRT1) from the cell cycle and apoptosis regulator 2 (CCAR2). Consequently, the depletion of the microbiota by antibiotics causes upregulation of Snhg9 and accelerates CRC progression. Moreover, Snhg9 is functionally conserved. Human SNHG9 promotes tumor growth via the same mechanism as mouse Snhg9 , despite their low sequence similarity.
研究领域:
医学
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分子生物学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
Nanoscience and Nanotechnology Letters
·
2017
·Vol. 9
·期 6
·941-944
·
IF 1.889
Nie Libo,Liu Fuhua,Yang Hongcheng
CdTe quantum dots (QDs) encoded polystyrene (PS) microbeads were synthesized in this article. Two or three kinds of QDs with different volume ratios were mixed. The PS microbeads were conjugated with the mixed QDs based on the coupling reaction of the carboxyl groups of QDs and the
amino groups on the surface of PS microbeads. The prepared QDs encoded PS beads shown specific fluorescence emission wavelengths and fluorescence intensities, which could be used as the support of specific bio-recognition in liquid biochips.
研究领域:
材料科学
·
生物技术
·
分析化学
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DOI
高影响因子
ACS Applied Optical Materials
·
2023
·
IF 0
Yingzhen Wu,Lin Lin,Pan Lu,Wenjun Ma,Zheng Li,Mingze Zhang,Yuanming Yang,Niuniu Ju,Yanan Zhang,Huanjie Liao,Zhuohong Feng,Zhezhe Wang,Zhiqiang Zheng
Up until now, the applications of lanthanide ion-doped lead-free double perovskite Cs2AgInCl6 nanocrystals (NCs) have been rarely reported, especially in fluorescence anticounterfeiting and coding. Here, we successfully incorporated Tb3+/Ho3+ ions into Cs2AgInCl6 NCs by the hot-injection method. Benefiting from the energy transfer process, Tb3+- and Ho3+-codoped Cs2AgInCl6 NCs showed tunable emission color in a green-blue-orange triangle region. Combining our NCs with transparent ink for printing, we put forward the applications of color bar codes and color QR codes (3D codes) as well as information encryption. Compared with traditional codes, color bar codes and 3D codes conquer the shortcomings of low information density, weak information security, and poor aesthetics. Furthermore, through double-mode excitation, we list an encoding table corresponding to 26 letters and 10 numbers. The encoding table embodies the application value of information encryption and coding.
研究领域:
材料科学
·
光学
·
信息安全
✓ 已验证
DOI
危险品化学品经营许可证（带存储）