INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jingjing Xie,Nansheng Wu,Dongming Li,Shiyi Xiong,Jinjiao Dong,Ruiling Wang,Guodong Zheng,Jingen Li
Iron deficiency anemia (IDA) is a prevalent nutritional deficiency problem. This study aimed to investigate the characteristics of Choerospondias axillaris polysaccharide-Fe (III) complex and its effect on IDA mice. CAP-Fe (III) complex was synthesized by co-thermal synthesis method with an iron content of 27.31 ± 0.97 %. The chelation of Fe 3+ reduced the crystallinity, increased the thermal stability and changed the morphological features of CAP, which was evidenced by XRD, TG, and SEM analysis. The probable existence of the β -FeOOH structure was supported by the characteristic absorption peaks in the FT-IR, XRD, and Mössbauer spectra. The CAP-Fe (III) complex showed a therapeutic effect on IDA mice, confirmed by improved erythroid parameters, iron levels, and oxidative stress. The results of gene expression levels determined by RT-qPCR demonstrated that the CAP-Fe (III) complex could regulate iron metabolism homeostasis, alleviate liver inflammation, and enhance intestinal barrier function. Furthermore, the dysbiosis of intestinal microbiota induced by IDA was effectively restored by CAP-Fe (III) complex, through enriching beneficial bacteria ( Firmicutes , Blautia , Oscillibacter , Colidextribacter ) and depleting harmful bacteria ( Actinobacteria , Proteobacteria , Parasutterella , Faecalibaculum , Escherichia-Shigella ). These findings suggested that CAP-Fe (III) may serve as a novel iron supplement for treating IDA.
研究领域:
营养学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·Vol. 21
·期 8
·e202302059
·
IF 2.3
Si-Mei Liu,Chong-Ying Liu,Zi-Long Chen,Yan Fang,Fang-Zheng Jiao,Li-Hua Zhang,Ting-Ting Zhang,Peng Zhao
This study extracted and purified a polysaccharide from Rehmanniae radix praeparata (RGP) with an average molecular weight. The structural characteristics of RGP and its iron (III) complex, RGP−Fe(III), were examined for their antioxidant properties and potential in treating iron deficiency anemia (IDA). Analysis revealed that RGP comprised Man, Rha, Gal, and Xyl, with a sugar residue skeleton featuring 1→3; 1→2, 3; and 1→2, 3, 4 linkages, among others. RGP−Fe(III) had a molecular weight of 4.39×10 4 Da. Notably, RGP−Fe(III) exhibited superior antioxidant activity compared to RGP alone. In IDA rat models, treatment with RGP−Fe(III) led to increased weight gain, restoration of key blood parameters including hemoglobin, red blood cells, and mean hemoglobin content, elevated serum iron levels, and decreased total iron-binding capacity. Histological examination revealed no observable toxic effects of RGP−Fe(III) on the liver and spleen. These findings suggest the potential of RGP−Fe(III) as a therapeutic agent for managing IDA and highlight its promising antioxidant properties. Graphical
研究领域:
中药学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Xiangjun Ding,Mengdan Xu,Haili Li,Xueling Li,Meiqing Li
There is an increasing amount of research into the development of a third generation of iron supplementation using peptide-iron chelates. Peptides isolated from mung bean were chelated with ferrous iron (MBP-Fe) and tested as a supplement in mice suffering from iron-deficiency anemia (IDA). Mice were randomly divided into seven groups: a group fed the normal diet, the IDA model group, and IDA groups treated with inorganic iron (FeSO 4 ), organic iron (ferrous bisglycinate, Gly-Fe), low-dose MBP-Fe(L-MBP-Fe), high-dose MBP-Fe(H-MBP-Fe), and MBP mixed with FeSO 4 (MBP/Fe). The different iron supplements were fed for 28 days via intragastric administration. The results showed that MBP-Fe and MBP/Fe had ameliorative effects, restoring hemoglobin (HGB), red blood cell (RBC), hematocrit (HCT), and serum iron (SI) levels as well as total iron binding capacity (TIBC) and body weight gain of the IDA mice to normal levels. Compared to the inorganic (FeSO 4 ) and organic (Gly-Fe) iron treatments, the spleen coefficient and damage to liver and spleen tissues were significantly lower in the H-MBP-Fe and MBP/Fe mixture groups, with reparative effects on jejunal tissue. Gene expression analysis of the iron transporters Dmt 1 ( Divalent metal transporter 1 ), Fpn 1 ( Ferroportin 1 ), and Dcytb ( Duodenal cytochrome b ) indicated that MBP promoted iron uptake. These findings suggest that mung bean peptide-ferrous chelate has potential as a peptide-based dietary supplement for treating iron deficiency.
研究领域:
营养学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Xiang-Rong Cheng,Yu-Yao Chen,Cong-Cong Cao,Jia-Hui Ma,Chen-Xi Zhang,Zi-Wei Zhao,Qian Wu,Yuan-Yuan Li,Mitchell N. Muskat,Guo-Qing Wu
Colla Corii Asini (Ejiao) peptide-iron (EPI) chelates formed during digestion have been revealed as potential hematopoietic components. However, it remains unclear how EPI regulates endogenous metabolism in vivo. Iron deficiency anemia (IDA) mice were gavaged with EPI chelates in a gradient dosage, while Ejiao hydrolysate-iron (EJI) chelates and FeSO 4 were used as positive controls. The effect of EPI on hematopoietic recovery was evaluated. For the plasma untargeted metabolomic analysis, liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied to identify metabolites in the samples. EPI significantly increased the levels of hemogram in IDA mice in a dose-dependent manner. The relative iron bioavailability in EPI was 123.45% of that in FeSO 4 in vivo . After high dose of EPI (EPI-H) supplementation, the levels of 22 potential metabolic markers in the IDA group returned to normal, including sphingomyelin and arachidonic acid . EPI-H affected endogenous metabolites by regulating the metabolism of energy, lipids , and amino acids , promoted hematopoiesis , regulated metabolic pathways, and improved iron homeostasis . It’s a comprehensive nutritional intervention of IDA by EPI supplementation.
研究领域:
营养学
·
代谢组学
·
血液学
✓ 已验证
产品关联
DOI
高影响因子
LIFE SCIENCES
·
2024
·
IF 5.2
Sijia Liu,Fang Guo,Tianli Zhang,Ying Zhu,Meng Lu,Xiayu Wu,Fuqin He,Ruiying Yu,Dan Yan,Zhangyin Ming,Dan Shu
Aims This research aimed to study the changes in platelet function and their underlying mechanisms in iron deficiency anemia. Main methods Initially, we evaluated platelet function in an IDA mice model. Due to the inability to accurately reduce intracellular Fe 2+ concentrations, we investigated the impact of Fe 2+ on platelet function by introducing varying concentrations of Fe 2+ . To probe the underlying mechanism, we simultaneously examined the dynamics of calcium in the cytosol, and integrin αIIbβ3 activation in Fe 2+ -treated platelets. Ferroptosis inhibitors Lip-1 and Fer-1 were applied to determine whether ferroptosis was involved in this process. Key findings Our study revealed that platelet function was suppressed in IDA mice. Fe 2+ concentration-dependently facilitated platelet activation and function in vitro. Mechanistically, Fe 2+ promoted calcium mobilization, integrin αIIbβ3 activation, and its downstream outside-in signaling. Additionally, we also demonstrated that ferroptosis might play a role in this process. Significance Our data suggest an association between iron and platelet activation, with iron deficiency resulting in impaired platelet function, while high concentrations of Fe 2+ contribute to platelet activation and function by promoting calcium mobilization, αIIbβ3 activation, and ferroptosis.
研究领域:
血液学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2024
·
IF 11.4
Pei-Yu Dong,Shuai Yuan,Yu-Mei Chen Yan,Yu Chen,Yue Bai,Yang Dong,Yin-Yin Li,Wei Shen,Xi-Feng Zhang
Iron deficiency is a common nutritional issue that seriously affects male reproductive health. Lotus root polysaccharide iron (LRPF), a novel nutritional supplement, may ameliorate the damage caused by iron deficiency, however, the mechanism is unclear. In this study, we comprehensively determined the benefits of LRPF on reproduction in iron-deficient mice by integrating transcriptomics, microbiomics and serum metabolomics. Microbiomics showed that LRPF could restore changes to the intestinal microbiota caused by iron deficiency. Metabolomics results showed that LRPF stabilised steroid hormone and fatty acid metabolism in iron-deficient mice, reduced the content of ethyl chrysanthemumate (EC) and ameliorated the reproductive impairment. The transcriptomic analysis showed that LRPF regulated steroid hormone synthesis and the peroxisome proliferator-activated receptor (PPAR) signalling pathway in iron-deficient mice. In vitro experiments showed that LRPF could promote steroid hormone synthesis in Leydig cells by activating PPARγ. In conclusion, this study highlights the advantage of LRPF to improve testicular development.
研究领域:
营养学
·
生殖生物学
·
分子生物学
·
转录组学
·
代谢组学
·
微生物组学
✓ 已验证
DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2023
·Vol. 120
·期 27
·e2304306120
·
IF 11.1
Xiao-Dong Sun,Jing-Ya Ma,Li-Juan Feng,Jian-Lu Duan,Xiao-Min Xie,Xiao-Han Zhang,Xiangpei Kong,Zhaojun Ding,Xian-Zheng Yuan
Understanding the fundamental interaction of nanoparticles at plant interfaces is critical for reaching field-scale applications of nanotechnology-enabled plant agriculture, as the processes between nanoparticles and root interfaces such as root compartments and root exudates remain largely unclear. Here, using iron deficiency–induced plant chlorosis as an indicator phenotype, we evaluated the iron transport capacity of Fe3O4 nanoparticles coated with citrate (CA) or polyacrylic acid (PAA) in the plant rhizosphere. Both nanoparticles can be used as a regulator of plant hormones to promote root elongation, but they regulate iron deficiency in plant in distinctive ways. In acidic root exudates secreted by iron-deficient Arabidopsis thaliana, CA-coated particles released fivefold more soluble iron by binding to acidic exudates mainly through hydrogen bonds and van der Waals forces and thus, prevented iron chlorosis more effectively than PAA-coated particles. We demonstrate through roots of mutants and visualization of pH changes that acidification of root exudates primarily originates from root tips and the synergistic mode of nanoparticle uptake and transformation in different root compartments. The nanoparticles entered the roots mainly through the epidermis but were not affected by lateral roots or root hairs. Our results show that magnetic nanoparticles can be a sustainable source of iron for preventing leaf chlorosis and that nanoparticle surface coating regulates this process in distinctive ways. This information also serves as an urgently needed theoretical basis for guiding the application of nanomaterials in agriculture.
研究领域:
植物科学
·
纳米技术
·
农业
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrition & Metabolism
·
2022
·Vol. 19
·期 1
·1-9
·
IF 4.654
Qiu Fubin,Li Rui,Gu Siyu,Zhao Yimin,Yang Linxue
Background Iron and vitamin D (VD) is essential to health. Previous studies have shown that iron homeostasis has a potential effect on VD metabolism, but the mechanism is not fully understood.ObjectivesTo explore the relationship between VD metabolism and iron metabolism, as well as the regulatory mechanism of iron on VD metabolism.Methods 40 male rats were fed adaptively for 7 days and randomly divided into control (C, n = 6 normal diet) group and model (M, n = 24 iron deficient diet) by simple randomization, the latter was used to establish iron deficiency anemia (IDA) model. After 6 weeks of feeding, the M group was randomly divided into: iron deficiency group (DFe), low iron group (LFe), medium iron group (MFe) and high iron group (HFe) by block randomization. Different doses of iron dextran (based on iron content (100 g·bw·d)): 0, 1.1, 3.3 and 9.9 mg) were given respectively. After 4 weeks, the rats were anesthetized with 8% chloral hydrate, Blood (collected from the abdominal aorta), liver and kidney tissues were collected. The serum and tissues were separately packed and frozen at -80℃ for testing.Results The results showed that the levels of hemoglobin (Hb), red blood cell (RBC), serum iron (SI), liver iron, and kidney iron in DFe group were lower than those in the other four groups, while the levels of total iron-binding capacity (TIBC), transferrin (TF) and transferrin receptor (Tfr) in DFe group were higher than those in other groups; The serum levels of 25-(OH)D3 and 1,25-(OH)2D3 in DFe group were significantly lower than those in C group (P < 0.05). The correlation analysis showed that the levels of 25-(OH)D3 and 1,25-(OH)2D3 were negatively correlated with TIBC, TF and Tfr no correlation with SI. Western blotting, immunofluorescence, and q-PCR results showed that compared with C group, the protein and gene expressions of CYP2R1, CYP27A1, and CYP24A1 in DFe group were down-regulated, and the expression of CYP27B1 protein and gene was up-regulated in DFe group.Conclusion Iron may be involved in the metabolism of VD3 by regulating the expression of VD3 hydroxylase, suggesting that appropriate iron supplementation might promote the activation of VD3.
研究领域:
营养学
·
生物化学
·
药理学
·
动物生理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xueqing He,Songyi Lin,Lei Chen,Yihan Huang,Jinhui Hu,Na Sun
Iron fortification with food supplements remains the primary dietary strategy for improving iron deficiency anemia (IDA). This study used Antarctic krill protein for fibrillar design to form an Antarctic krill protein amyloid fibril (AKAF). The results indicated that peptides generated by proteolysis were a prerequisite for fibril assembly, forming elongated fibril structures and cross-linking upon heating. During this process, hydrogen bonds were rearranged, forming ordered β-sheet conformations (49.36 ± 0.21%); π–π stacking interactions among aromatic residues contributed to fibril formation. Further studies showed that AKAF effectively maintained iron in a bioavailable state and exhibited a high binding capacity (60.67 ± 0.69%). Moreover, the AKAF-iron complex markedly ameliorated hematological abnormalities in IDA mice, enhanced iron storage in the liver and spleen, and positively influenced the expression of iron homeostasis genes. This complex was also effective in alleviating gastric inflammatory responses induced by IDA. Overall, AKAF holds promise as an efficient iron delivery carrier.
研究领域:
营养学
·
生物化学
·
食品科学
·
分子生物学
·
医学研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jing Jing,Zhijun Zhang,Zhiyong Hu,Xuemei Ma,Jianlan Cui,Hailin Zhu,Xueli Bai,Lijun Zhai
Iron deficiency anemia (IDA) is the most common nutritional disease worldwide. In this study, a low methoxyl pectin (LMP)‑iron(III) complex was prepared. The physicochemical and structural properties were characterized by HPSEC, HPIC, CV, FTIR, 1 H NMR, XRD, SEM and CD. The results showed that iron increased the molecular weight of the LMP‑iron(III) from 11.50 ± 0.32 to 12.70 ± 0.45 kDa and improved its crystallinity. Moreover, the findings demonstrated that -OH and -COOH groups in LMP coordinate with Fe 3+ to form β -FeOOH. The water-holding capacity, emulsion stability, and antioxidant activities of the LMP‑iron(III) were lower than those of LMP. Furthermore, the therapeutic effects of LMP‑iron(III) on IDA were investigated in rats. Following LMP‑iron(III) supplementation, compared with the model group, the administration of LMP‑iron(III) significantly increased the body weight, hemoglobin concentration, and serum iron concentration as well as decreased free erythrocyte protoporphyrin concentration. Therefore, the LMP‑iron(III) can potentially treat IDA in rats experiments, providing a theoretical basis for the development of a promising iron supplement.
研究领域:
营养学
·
生物化学
·
药学
·
动物科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2024
·Vol. 26
·期 11
·1-14
·
IF 2.1
Ma Yuying,Wu Hai,Jia Min,Zhang Zhijun,Wang Jingwei,Yue Zhenggang,Wu Hong,Yang Tiehong
This investigation is aimed to develop a novel iron supplement, iron oxide nanoparticles modified with Angelica sinensis polysaccharide (IONPs-ASP), for the treatment of iron deficiency anemia (IDA). IONPs-ASP was synthesized using the co-precipitation method and characterized for their structural features, physicochemical properties, and biocompatibility by TEM, SEM, FTIR, etc. The therapeutic effects of IONPs-ASP were assessed in a rat IDA model. IONPs-ASP was administered intravenously, and blood parameters were monitored. In addition, the preliminary safety of IONPs-ASP was evaluated by organ coefficients and histopathological staining. IONPs-ASP was successfully constructed, with a small particle size, core–shell structure, hydrophilicity, and good biocompatibility. Compared to unmodified IONPs, IONPs-ASP showed better stability and dispersion. In the IDA rat model, IONPs-ASP exhibited superior therapeutic efficacy than that of ASP or IONPs monotherapy. Furthermore, no abnormalities were observed in the organ coefficients and tissue section. IONPs-ASP not only has a superior therapeutic effect on IDA but also has the effect of the ASP on supplementing blood. Hence, it may be used as a new iron supplementing agent with double therapeutic efficacy on blood supplementation for the treatment of IDA. Graphical Abstract In this study, we constructed Angelica sinensis polysaccharide (ASP)–modified iron oxide nanoparticles (IONPs) and demonstrated the therapeutic effects of IONPs-ASP on iron deficiency anemia (IDA), which was associated with IONPs supplementation and APS-stimulated hematopoietic cell generation.
研究领域:
纳米技术
·
药理学
·
生物医学工程
·
血液学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）