Food & Function
·
2019
·Vol. 10
·期 2
·1123-1131
·
IF 3.241
Dongmei Li,Xiaokang Na,Wanru Zhou,Congcong Wang,Yuliang Li,Bei-Wei Zhu,Mingqian Tan
The effect of endogenous carbon nanoparticles from food sources is one of the hot topics in the current food research field. The relationship between the foodborne nanoparticle properties and the cytotoxic mechanism has been insufficiently studied. In this work, carbon dots (CDs) with strong fluorescence were found and purified from canned yellow croaker, and their cytotoxicity was investigated for the first time. The canned yellow croaker CDs are nearly spherical with a particle size distribution in the range of 1.8–5.8 nm. The fluorescence quantum yield of the isolated CDs is 9.7% and the maximum excitation wavelength is 340 nm, with a significant redshift phenomenon in fluorescence spectra. The surface elemental analysis showed that the composition of the canned yellow croaker CDs was C (76.42%), N (6.49%), and O (16.7%), and various functional groups are on the surface. The CDs have good stability in sodium chloride solution and the fluorescence intensity was stable within the pH value of 4 to 10. A strong fluorescence quenching effect was found upon the addition of Cu2+ and Fe3+ to the CD aqueous solution. The CDs can easily enter the interior of the live cells. Moreover, a concentration-dependent behavior of HepG2 cell viability was found when the cells were incubated with the canned yellow croaker CDs. Glycolysis and mitochondrial function analysis of HepG2 cells revealed that both the extracellular acidification rate and oxygen consumption rate significantly decreased in contrast to the normal level prior to the addition of CDs. In addition, the CDs significantly inhibited the glycolytic pathway by reducing the activity of key enzymes hexokinase and pyruvate kinase in the glycolytic pathway.
研究领域:
食品科学
·
毒理学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2019
·Vol. 43
·期 10
·e14111
·
IF 1.288
Yiqi Zhang,Xuting Ma,Zhiyuan Dai
Yellowfin tuna is one of the favorite aquatic foods and possesses high economic value due to its unique pleasant flavor and good nutrition quality. Volatile and nonvolatile compounds that affect the flavor quality of tuna fish during canning process were investigated. The volatiles were analyzed by headspace solid-phase microextraction combined with GC-MS and the nonvolatiles were characterized by free amino acids, 5′-nucleotides, and organic acids. Histidine, lysine, inosinic acid, tartaric acid, and succinic acid were the key taste compounds in tuna fish. A total of 7, 9, and 7 aroma-active compounds were identified in raw, cooked, and canned fish samples, respectively. Decanal, nonanal, octanal, and (E)-2-nonenal were the predominant odorants in raw samples. Octanal, nonanal, 2-pentylfuran, 1-octen-3-ol, hexanal, and 2-ethylfuran contributed to the characteristic flavor of cooked samples. 2-Methyl-3-furanthiol, octanal, and nonanal were the main aroma-active compounds detected in the canned samples responsible for the meaty and fatty odor. Practical applications Yellowfin tuna is one of the most commercial fish species for its excellent economic and nutritional value. The tuna fish processing methods are mainly frozen, cooked, smoked, and canned after thermal processing. The flavor components of tuna would vary greatly depending on the processing method. Although quality characteristics are very important for the economic value of tuna, there is scarce information comparing the volatile and nonvolatile compounds in tuna meat during canning process. This research could provide basis data for the process and the quality improvement of tuna products.
研究领域:
食品科学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF DRUG TARGETING
·
2021
·
IF 5.121
Yongwei Hao,Cuixia Zheng,Qingxia Song,Hongli Chen,Wenbin Nan,Lei Wang,Zhenzhong Zhang,Yun Zhang
Drug delivery with the help of nanoparticles could transport more payloads to tumour site. Owing to their limited accumulation and penetration in the tumour tissues, to increase delivery efficiency is currently still required for applying nanomedicine to treat tumour. Here, we initially report a pressure-driven accumulation of drug-loaded nanoparticles to tumours for efficient tumour therapy with a dry cupping device. The mesoporous Mn-doped silica based nanoparticles delivering 5-aza-2-deoxycytidine and docetaxel were prepared, characterised and used as a model nanomedicine to investigate the potential of dry cupping treatment. For this system, the Mn doping not only endowed the mesoporous silica nanoparticles biodegradability, but also made it much easier to bind a tumour targeting group, which is a G-quadruplex-forming aptamer AS1411. On tumour-bearing mice, the in vivo results demonstrated that the dry cupping treatment could substantially improve the distribution of nanomedicines at tumour site, resulting in enhanced treatment efficacy. Overall, this method enables the therapeutical nanoparticles accumulate to tumour through increasing the blood perfusion as well as altering the biological barrier, which opened up possibilities for the development of pressure-driven nanomedicine accumulation at tumour site.
研究领域:
药学
·
生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2017
·
IF 1.427
Tianzhu Guan,Tiezhu Li,Tiehua Zhang,Zhuolin Li,Yongzhi Wang,Hansong Yu,Ping Ruan,Jie Zhang,Yongjun Wang
This work developed a fluorescence polarization (FP) assay for simultaneous monitoring BPA, BPF, BADGE, and BFDGE in canned tuna. The assay was based on the competitive binding between bisphenol analogues (BPs) and dexamethasone fluorescein (Dex-fl) for mouse peroxisome proliferator-activated receptor α ligand binding domain (mPPARα-LBD*). The soluble form of mPPARα-LBD* was expressed in Escherichia coli strain Rosetta (DE3), as a recognition element on detection of BPs. Under optimized conditions, four bisphenol analytes were detected at concentrations corresponding to the specific migration limits (SMLs) as required by the European Union. The FP assay showed IC50 values of 2.80, 6.51, 1.72, and 4.28 mg L−1 with a limit of detection of 0.35, 0.08, 0.10, and 0.49 mg L−1 for BPA, BPF, BADGE, and BFDGE, respectively. The analysis of spiked canned tuna samples showed the acceptable recoveries ranged from 87.9% to 77.3%, with variation coefficients ranging from 9.0% to 15.8%. With the high sensitivity and wide-range affinities to BPs, the developed assay based on mPPARα-LBD* exhibited the potential to be a screening assay for fast detection of BPs in canned tuna.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Qingkui Fang,Shiyu Li,Tingting Wang,Mingjin Zhong,Baoting Nie,Kang Zheng,Xiujun Tang,Jinjing Xiao,Min Liao,Haiqun Cao
Alternaria spp. infect and accumulate various toxic secondary metabolites in apples, posing a threat to human and animal health. This study established instrumental analytical methods for six Alternaria toxins, systematically investigated the contamination levels of Alternaria toxins in apples and their reduction and transfer patterns during processing. Apples exhibited heightened disease susceptibility at 25 °C and produced 6 Alternaria toxins in different degrees, while Alternaria toxins were not observed within 4 cm of the symptomatic site. The transfer rate to pomace after juice extraction was higher than that in juice, with transfer rates ranging from 51.9 to 62.75 % for pomace and from 21.44 to 35.79 % for juice. Canning reduced Alternaria toxins in pulp, with the highest reduction rate of 93.3 % for tenuazonic acid. A total of four Alternaria toxins were detected in the testing of market samples of apple products, and none of the assessed total daily exposure doses of toxins ingested by humans through apple products exceeded the daily intake level recommended by the EFSA. The evaluation results indicate that the risk of dietary exposure of humans is relatively low through apple products. These findings provide valuable data and theoretical support for ensuring food safety in apples.
研究领域:
食品科学
·
毒理学
·
植物病理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Jin-Yu Qiao,Yue-Hong Pang,Zhu-Ying Yan,Xiao-Fang Shen
Bisphenols (BPs), one of the endocrine disrupting chemicals (EDCs), are harmful to health with the potential carcinogenicity, teratogenicity and neurotoxicity. In this paper, we developed an electro-enhanced solid-phase microextraction with membrane protection method to enrich BPs. The extraction time can be shortened to 10 min with the electric field, and the prepared fiber can be repetitively used 100 times with the membrane protection. The factors affecting extraction such potential, pH, extraction temperature and extraction time were optimized. Under optimal conditions, the linear ranges were 2-600 ng mL −1 for BPAF and 2-800 ng mL −1 for the other four BPs (BPB, BPF, BPA, BPZ), and the limits of detection (S/N=3) were from 0.09 (BPAF) to 0.41 (BPA) ng mL −1 , with intra-day and inter-day precisions <5.16% and 9.29%, respectively. The developed method has been applied to the determination of trace BPs in canned meat with the recoveries of 73%-117%, showing a promising application for pretreatment in complex samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Jinlong Wu,Changwen Chen,Yong Li,Ke Cao,Xinwei Wang,Weichao Fang,Gengrui Zhu,Lirong Wang
The characterization of peach metabolites and carotenoids during canning and storage remains unclear. The present study identified 658 metabolites and 40 carotenoids in peach fruits throughout the canning and storage using ESI-MS/MS and APCI-MS/MS based metabolome approach. A total of 282 differentially accumulated metabolites were found, mainly including 78 phenolic acids, 74 lipids, 61 flavonoids. Five esterified carotenoids (rubixanthin palmitate, β-cryptoxanthin oleate, β-cryptoxanthin laurate, β-cryptoxanthin palmitate, and β-cryptoxanthin myristate) were the main peach carotenoids, with a proportion of approximately 90%, while free carotenoids accounted for 4.22–5.95% during the entire processing period. Moreover, the total carotenoid loss rates for canning and storage were 56.67% and 46.55%, respectively. Compared to the loss of free carotenoids, esterified carotenoids were more stable during storage, while canning led to a greater loss of esterified carotenoids. The results provided new insights into the maintenance of health-related phytochemicals from canning processes.
研究领域:
食品科学
·
代谢组学
·
农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2020
·Vol. 246
·期 9
·1857-1865
·
IF 2.366
Yang Fanfan,Xu Long,Liu Xiang,Chen Chen,Dias Alberto C. P.,Zhang Xiaoying
Abstract Bisphenol S (BPS) is a major alternative to Bisphenol A (BPA) newly applied in food packing material and disrupts reproductive and developmental endpoints through endocrine pathways. This study aimed to develop a rapid, sensitive, and accurate chemiluminescence immunoassay (CLIA) for the detection of BPS in canned beverage. Anti-BPS monoclonal antibody (mAb) was generated by immunizing Balb/c mice with immunogen (BPS–BSA conjugation). CLIA assay conditions, including 3-[9-(((3-( N -succinimidyloxycarboxypropyl) [4-methxylphenyl] sulfonyl) amine) carboxyl]-10-acridiniumyl)-1-propanesulfonate inner salt (NSP-SA-NHS) labeling ratio (1:40), antigen concentration (1 μg mL −1 ) and antibody titer (1:8000; 125 ng mL −1 in PBS (0.01 M, pH 7.4)) were determined, respectively. The half inhibition concentration (IC 50 ), detection limit (IC 10 ), and working range of the CLIA in canned beverage were 0.04, 1.48, and 0.32–5 ng mL −1 , respectively. Cross-reactivity with other bisphenols (4,4′-thiodiphenol, Bisphenol F, 4,4′- dihydroxy benzophenone, Bisphenol A and Bisphenol B) was less than 0.56%, and the recovery rates of BPS in canned beverage ranged from 80 to 109.2% (intra-assay) and from 82 to 108.4% (inter-assay). The results of CLIA were compared with high-performance liquid chromatography–UV (HPLC–UV) analysis, showing a good correlation of 0.956. After proper sample pre-treatment, this method allows relatively large amounts of sample analysis in a limited time period (30 min). Thus, it was deemed suitable and efficient for routine screening of BPS in canned beverage. Graphic abstract
研究领域:
分析化学
·
环境科学
·
食品安全
·
免疫学
·
毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）