POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
·
IF 6.8
Jiayi Song,Maomao Ma,Yijian Wu,Yan Hu,Xinyi Wu,Peipei Guan,Bin Zeng
Green mold, caused by Penicillium digitatum , is one of the most destructive postharvest diseases of citrus fruits, leading to significant rot and economic losses. In this study, the antifungal activity and underlying mechanism of the novel antimicrobial peptide (AMP) N1–6L7A against P. digitatum were investigated. Additionally, the potential application of N1–6L7A for controlling postharvest green mold in Citrus sinensis (L.) Osbeck was examined. N1–6L7A exhibited potential antifungal efficacy by inhibiting both mycelial growth and spore germination of P. digitatum . Furthermore, it demonstrated excellent thermal stability, pH tolerance, and biosafety. The results of fluorescence microscopy assay, cell component leakage, and microscopic observations revealed that N1–6L7A severely damaged the fungal membrane of P. digitatum , leading to the release of intracellular nucleic acids and proteins. N1–6L7A treatment also induced excessive intracellular reactive oxygen species (ROS) production, collapse of mitochondrial membrane potential, and decreased activities of malate dehydrogenase (MDH) and ATPase. Gel retardation assay indicated that P. digitatum -DNA was a target of N1–6L7A. Furthermore, transcriptomic analysis suggested that N1–6L7A might inhibit the growth of P. digitatum by blocking the expression of genes related to the ribosome, amino acid metabolism, and pyrimidine metabolism. Meanwhile, the peptide potentially promoted the expression of genes involved in steroid biosynthesis. Notably, in vivo assays confirmed that N1–6L7A effectively controlled P. digitatum infection in citrus fruits and enhanced host resistance by activating defense-related enzymes. Collectively, our results suggested that N1–6L7A could be used as an alternative fungicide to prevent green mold in citrus fruits.
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Foods
·
2026
·Vol. 15
·期 16
·2867
·
IF 6
Peipei Ma,Runrun Zhang,Chen Li,Qiao He,Xinhui Zhang,Zhixiang Cai
Staphylococcus aureus(S. aureus) is a prominent foodborne pathogen that poses a continuous threat to global public health and food safety due to its possession of a variety of toxins and its multidrug resistance. Medium-chain fatty acids (MCFAs), notably lauric acid (LA), exhibit strong antimicrobial properties, but their application is heavily constrained by poor water solubility. In this study, optimized LA emulsions stabilized by chitosan (CS) and polyvinyl alcohol (PVA) were evaluated for their antibacterial activity and detailed mode of action againstS. aureusATCC 6538. The antibacterial activities were evaluated by the maximum inhibition zone, with the 20 CS-PVA/DLTA-LA formulation exhibiting stable dispersion and potent antibacterial activity at 1%. The underlying antibacterial mechanisms againstS. aureuswere specifically focused on cell membranes and peptidoglycan. Therein, the binding of emulsion droplets to the anionic bacterial surface was driven by electrostatic attraction. Membrane degradation was also observed with membrane dysfunctions involving membrane depolarization, increased permeability, and fluidity reduction triggered by their subsequent insertion into the lipid bilayer, which may cause cell dysmetabolism, disintegration, and eventual cell death. Overall, these findings substantiate that LA emulsions disruptS. aureusby operating potentially multi-targeted effects involving cell membrane disruption and peptidoglycan interference, offering a promising alternative approach warranting further investigation for foodborne pathogen control.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Chen Li,Peipei Ma,Xiaoyue Sui,Aminu Abdullahi Mahmoud,Xinyu Liao,Tian Ding,Runrun Zhang
The stable food-grade lauric acid emulsions were developed. • Emulsions showed homogeneous structure and good viscoelastic stability. • The emulsions exhibited notable antioxidant activity and activity against S. aureus . • Antibacterial activity linked to membrane damage and energy disruption. Lauric acid (LA) is an antimicrobial medium-chain fatty acid, but its poor aqueous solubility limits its application in food matrices. To address this, ultrasound-assisted emulsion was developed using LA in vitamin E (VE) oil and chitosan-polyvinyl alcohol (CS-PVA) aqueous matrix. The emulsion exhibited uniformly dispersed oil droplets forming a compact network structure, along with superior stability and viscoelastic properties. No phase separation was observed during storage except for the 5 CP/VL system after 30 days, where CP/VL refers to the CS-PVA/VE-LA. Functionally, the emulsion disrupted bacterial membrane integrity and interfered with cellular energy metabolism. The 10 CP/VL formulation achieved a favorable balance between stability and bioactivity, with DPPH scavenging rate of 26.57% and 2.8 log CFU/mL reduction of Staphylococcus aureus after 2 h at 1% concentration. Overall, this study provides a feasible strategy for enhancing the dispersion and function of LA in aqueous systems for food-related applications. Download: Download high-res image (399KB) Download: Download full-size image
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Biomaterials Research
·
2026
·
IF 9.8
Kai Dong,Zelin Guan,Danyang Wang,Jinyao Sun,Ying Zhang,Cuiyu You,Yuanming Xing
In the pathogenesis of ulcerative colitis (UC), inflammatory responses, mucosal injury, and gutdysbiosis form a mutually reinforcing network that perpetuates disease progression. Current clinicalinterventions can rarely modulate these pathological modules simultaneously, underscoring theurgent need for more effective therapeutic strategies. Here, we report a dual-functional nano-therapythat couples aryl-hydrocarbon-receptor (AhR) activation with on-site reactive oxygen species (ROS)scavenging. An amphiphilic copolymer, HA-TK-LA (HTL), was synthesized by grafting lipoic acid(LA) to hyaluronic acid (HA) via a ROS-cleavable thioketal (TK) linker. The copolymer self-assembles into ~130 nm nanoparticles that encapsulate the natural AhR ligand indole-3-acetic acid(IAA) with a high loading efficiency of 73%. The resulting IAA@HTL nanoparticles exhibitROS/glutathione (GSH)-triggered drug release, and are actively taken up by inflamed intestinalepithelial cells and M1 macrophages through CD44-mediated endocytosis, leading to suppressedepithelial apoptosis and macrophage repolarization toward an anti-inflammatory phenotype. Indextran sulfate sodium (DSS)-induced murine colitis, IAA@HTL specifically accumulates in theinflamed colon and markedly alleviates disease activity. Mechanistically, IAA and LA releasedfrom the nanoparticles synergistically activate AhR/CYP1A1 and Nrf2/HO-1 signaling, skewmacrophages toward an anti-inflammatory state, dampen pro-inflammatory cytokine release, andreinforce the mucosal barrier by up-regulating tight-junction proteins and IL-22 secretion. Asinflammation subsides and mucosal integrity is restored, the gut microbiota gradually returns tohomeostasis. Collectively, this study establishes a ROS-scavenging and AhR-activating nano-delivery system that achieves a “one-stone-three-birds” outcome—attenuating inflammation,repairing mucosa, and rebalancing microbiota—providing new experimental evidence andtheoretical support for UC therapy.Keywords: ulcerative colitis, AhR receptor, reactive oxygen species, active targeting, macrophages
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Journal of Environmental Chemical Engineering
·
2026
·
IF 7.5
Junwei Yao,Qian Wang,Chunxin Wang,Hong Qi,Qingshan Shi,Jingxia Liu,Jin Feng,Ping Yang,Manli Yu,Xiaobao Xie
Attributing to poor effectiveness and inadmissible toxicity, fungicide is a tendency to restricted in the field of agriculture. Therefore, it was a prospective scene to exploit the inventive antifungal active ingredient delivery system for improving biological activity and environmental safety. The excellent adhesive fungicide formulation was endowed with an effective tactic to promote wettability and bioavailability. The elemental intention of this study was to illuminate synergistic mechanisms of dopamine (DA) coated prochloraz (Pro)-polycaprolactone (PCL) nanospheres on prominent roles of improving antifungal activity and environmental safety. Apart from enhancing wettability and anti-photodegradation ability, Pro-PCL-DA nanospheres was also able to reduce the burst release rate and prolong release duration for the phenolic hydroxyl group in DA. The Pro-PCL-DA nanospheres was charged with the superior antifungal activity against Fusarium oxysporum . The predominant antifungal activity of Pro-PCL-DA nanospheres was predominantly ascribed to superior transmembrane transport capacity, inducing excessive reactive oxygen species and enhancing membrane permeability. The environmental safety assay indicated that Pro-PCL-DA nanospheres could effectively reduce the acute toxicity of Pro to adult zebrafish and Eisenia fetida . Overall, these findings indicated that DA coated Pro-PCL nanospheres was potential route to provide a bright platform for improving fungicide utilization with the long-term sustainable development of agriculture.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Haiyan Lin,Yiliang Cai,Jingjing Shan,Xinyu Mao,Zihan Zhang,Jianjun Huang,Chenggang Cai,Ruiyu Zhu
Light-emitting diode (LED) technology has emerged as an eco-friendly antimicrobial strategy for postharvest preservation. This study identified Cladosporium cladosporioides as a novel postharvest pathogen in Ehime Kashi No.28 citrus and systematically investigated the wavelength-dependent antifungal effects of LED irradiation. At 120 μmol m −2 s −1 intensity, white and blue spectra significantly suppressed C. cladosporioides mycelial growth by 35.5 % compared to the dark control, outperforming red/green wavelengths. Further investigation revealed that C. cladosporioides exposed to white and blue LED irradiation (120 μmol m −2 s −1 ) underwent apoptosis, concomitant with suppressed mycelial proliferation and conidial germination. These effects correlated mechanistically with a triad of cellular disruptions, including loss of membrane integrity, ultrastructural disorganization of organelles, and substantial oxidative stress-induced damage. Crucially, in vivo trials demonstrated 53.6 % (white LED irradiation) and 56.8 % (blue LED irradiation) reductions in citrus lesion diameters while maintaining fruit quality. The dual functionality of wavelength-specific pathogen suppression and quality preservation positions LED irradiation as a sustainable, non-thermal method for citrus postharvest management.
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Algal Research-Biomass Biofuels and Bioproducts
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2026
·
IF 4.5
Xibo Lu,Seyed Ali Johari,Zhuang Wang
The increasing prevalence of microplastics (MPs) in the aquatic environment poses a significant threat to aquatic organisms. However, research on their specific toxicological mechanisms remains limited. This study investigated the growth inhibition toxicity of 1 μm-sized polystyrene microplastics (PSMPs) at environmentally relevant concentrations (1.33 × 10 4 , 7.67 × 10 4 , 4.87 × 10 5 , and 2.78 × 10 6 particles/mL) to the microalga Tetradesmus obliquus and explored the physiological and transcriptional responses induced by PSMPs in the algae. Exposure to PSMPs did not significantly inhibit algal growth across all the concentrations. PSMPs at the highest concentration induced intracellular oxidative stress, leading to a statistically significant increase in reactive oxygen species levels, as well as elevated levels of superoxide dismutase and malondialdehyde. The decrease in mitochondrial membrane potential may serve as a signal for cell apoptosis triggered by oxidative damage caused by PSMPs. Exposure to PSMPs significantly altered the gene expression profile of T. obliquus , affecting biological processes such as cell differentiation, membrane integrity, material transport and ion regulation. Furthermore, PSMPs disrupted the sphingolipid metabolism pathway in T. obliquus , causing abnormal expression of key synthetic and degradative enzymes, which affected the structure and function of cellular membrane. Overall, these findings demonstrated that PSMPs not only induced oxidative stress responses in T. obliquus but also exerted adverse effects on the algae by disrupting their metabolic pathways and gene expression. This study provides novel molecular insights and ecological evidence regarding the impacts of MPs on aquatic organisms.
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JOURNAL OF NANOPARTICLE RESEARCH
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2025
·Vol. 27
·期 10
·1-16
·
IF 2.6
Zhu Junqiao,Tang Wei,Sun Zhiting,Zhu Xinhai,Quan Qiang,Yin Jiaxin,Huang Qiugui,Jia Guoxia,Zhao Yang,Tang Yuqin,Zhang Yan,Xie Linhao,Zhao Jianfu
Multiple cycles of transarterial embolization chemotherapy for hepatocellular carcinoma (HCC) can easily cause the overexpression of P-glycoprotein (P-gp), leading to multidrug resistance (MDR) of HCC. Herein, we linked PLA (polylactic acid) with doxorubicin (DOX) through acid-sensitive hydrazone bonds to prepare PLA-ADH/DOX. Then, PLA-ADH/DOX and disulfiram (DSF) were physically encapsulated into PLGA-PEG (polylactic-co-glycolic acid-polyethylene glycol) as the backbone to fabricate a drug delivery system (DOX/DSF NPs) by nanoprecipitation and emulsion solvent evaporation method. The data of physicochemical characterization showed that DOX/DSF NPs enabled excellent stability at room temperature and pH sensitivity in an acidic environment. More importantly, DOX/DSF NPs could avoid P-gp-mediated efflux through endocytosis pathways and inhibit the function of P-gp by DSF, which increases cellular uptake of DOX in HepG2/DOX cells (hepatocellular DOX-resistant cancer cell line) and promotes DOX to enter the nucleus of HepG2/DOX cells for exerting the therapeutic effect in vitro. In addition, DOX/DSF NPs could increase intracellular reactive oxygen species (ROS) levels and reduce intracellular adenosine triphosphate (ATP) levels, thereby further inhibiting the function of P-gp, increasing intracellular concentration of drugs, and ultimately overcoming MDR in HCC. Hence, the combination of DOX and DSF delivered by DOX/DSF NPs with pH-sensitive and sequentially controlled drug release may provide a new way for MDR of HCC.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Ruiyu Zhu,Weilun Wang,Zisheng Luo,Haiyan Lin,Yong Li,Weiqiang Lu,Zimu Xu,Chenggang Cai,Shuheng Hu
Blue light-emitting diode (LED) light has been considered to influence the growth, quality, and preservation of certain fruit and vegetable. However, the blue LED light impact on apple postharvest blue mold remains elusive. In this study, the influence of blue LED light on Penicillium expansum virulence and patulin secretion was investigated in vitro and in vivo, as well as on the apple quality. The growth of P. expansum was inhibited by blue LED light via changes in mycelium morphology changes, cell membrane integrity, and potential mitochondrial membrane destruction, thereby causing reactive oxygen species accumulation, oxidative stress , and mitochondrial dysfunction. Furthermore, the expression level of LaeA relevant to the pathogenicity of P. expansum , was down-regulated. Specifically, the patulin production of P. expansum was inhibited via expression down-regulation of the patulin biosynthesis-related genes PatA , PatE , and PatN rather than direct degradation by the blue LED light treatment. Additionally, no significant effects on the fruit quality were observed when the light intensity was lower than 120 μmol m -2 s -1 . In summary, blue LED light treatment has the potential for a low-cost, safe, and non-chemical approach to control apple postharvest decay.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 22
·63109-63120
·
IF 5.8
Yu Le,Wang Zhuang,Wang De-Gao
Layered double hydroxide (LDH) nanomaterials are utilized extensively in numerous fields because of their distinctive structural properties. It is critical to understand the environmental behavior and toxicological effects of LDHs to address potential concerns caused by their release into the environment. In this work, the toxicological effects of two typical LDHs (Mg-Al-LDH and Zn-Al-LDH) on freshwater green algae ( Scenedesmus obliquus ) and the main affecting factors were examined. The Zn-Al-LDH exhibited a stronger growth inhibition toxicity than the Mg-Al-LDH in terms of median effect concentration. This toxicity difference was connected to the stability of particle dispersion in water and the metallic composition of LDHs. The contribution of the dissolved metal ions to the overall toxicity of the LDHs was lower than that of their particulate forms. Moreover, the joint toxic action of different dissolved metal ions in each LDH belonged to additive effects. The Mg-Al-LDH induced a stronger oxidative stress effect in algal cells than the Zn-Al-LDH, and mitochondrion was the main site of LDH-induced production of reactive oxygen species. Scanning electron microscope observation indicated that both LDHs caused severe damage to the algal cell surface. At environmentally relevant concentrations, the LDHs exhibited joint toxic actions with two co-occurring contaminants (oxytetracycline and nano-titanium dioxide) on S. obliquus in an additive manner mainly. These findings emphasize the impacts of the intrinsic nature of LDHs, the aqueous stability of LDHs, and other environmental contaminants on their ecotoxicological effects.
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JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Xiaomei Liang,Yulu Yang,Chuanqing Huang,Zhibin Ye,Wujiang Lai,Jiamao Luo,Xiaoxuan Li,Xiao Yi,Jun-Bing Fan,Ying Wang,Yifeng Wang
Resistance to the chemotherapeutic agent cisplatin (DDP) is the primary reason for invalid chemotherapy of ovarian cancer. Given the complex mechanisms underlying chemo-resistance, the design of combination therapies based on blocking multiple mechanisms is a rationale to synergistically elevate therapeutic effect for effectively overcoming cancer chemo-resistance. Herein, we demonstrated a multifunctional nanoparticle ( [email protected] ), which could simultaneously co-deliver DDP and Olaparib (Ola, DNA damage repair inhibitor) using targeted ligand cRGD peptide modified with heparin (HR) as nanocarrier, enabling the concurrent tackling of multiple resistance mechanisms to effectively inhibit the growth and metastasis of DDP-resistant ovarian cancer. In combination strategy, heparin could suppress the function of multidrug resistance-associated protein 2 (MRP2) and P-glycoprotein (P-gp) to promote the intracellular accumulation of DDP and Ola by specifically binding with heparanase (HPSE) to down-regulate PI3K/AKT/mTOR signaling pathway, and simultaneously served as a carrier combined with Ola to synergistically enhance the anti-proliferation ability of DDP for resistant ovarian cancer, thus achieving great therapeutic efficacy. Our [email protected] could provide a simple and multifunctional combination strategy to trigger an anticipated cascading effect, thus effectively overcoming the chemo-resistance of ovarian cancer.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Jiali Qian,Hongli Li,Yuanhang Wang,Yixuan Li,Jie Yu,Lei Zhou,Qiaosheng Pu
Plastic microchips possess the advantages of easy fabrication and low-cost, but their surface properties are frequently incompatible with electrophoretic separation without proper surface modification. Meanwhile, the separation microchannels on typical microchips are usually only a few centimeters long, the pressurized flow may significantly affect the electrophoretic separation if their inner diameters (id) are relatively larger (approximately > 50 μm), viscous separation medium is therefore required for efficient separation. Herein, a zwitterionic surfactant, N-hexadecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (HDAPS), was used as a multifunctional additive to inhibit the analyte adsorption, improve the surface status, control Joule heating and modulate the resolution on cyclic olefin copolymer microchips with 80 μm id, 5 cm long separation microchannels, eliminating the necessity of viscous polymeric additives. The effectiveness of HDAPS was compared with an ionic polymeric additive, poly(diallydimethylammonium chloride). The streaming potential and electroosmotic flow measurements indicated an effective inhibition of the adsorption of rhodamine B and a stable negative surface charge with zwitterionic HDAPS. Using 15 mmol/L HDAPS, 40% (v/v) methanol, and 10 mmol/L boric acid (pH 3.2) as the running buffer, rapid separation of four rhodamines was achieved within 90 s under a separation electric field of 520 V/cm. The theoretical plate numbers were in a range of 5.0×10 5 –6.9×10 5 /m. The relative standard deviations were no more than 0.9% for retention time and 1.5% for peak area. The proposed system was verified by the determination of rhodamines in eyeshadow and wolfberry, with standard recoveries in a range of 98.2%–101.4%.
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