BIOMATERIALS
·
2026
·
IF 13.6
Zhenrong Zhu,Peiyang Gu,Xing Li,Xiaolin Xiao,Hongzhe Wang,Jie Liang,Yujiang Fan,Yong Sun
Macrophages serve as central units in immune regulation, with their polarization influencing distinct stages of the bone healing process. Here, we report an immune-adaptive 3D-printed scaffold that integrates engineered macrophage capture traps, an HA network-mediated macrophage anchoring interface, onto a poly(lactic-co-glycolic acid)/hydroxyapatite microsphere composite framework. These traps selectively enrich macrophages via CD44 receptor-mediated recognition and dynamically guide their polarization. Intramuscular implantation experiments demonstrated a sequential shift in polarization of scaffold-enriched macrophages from M1 at 3 days to M2 at 7 days. Omics analysis of the short-term rabbit calvarial defect model revealed that HA released from this scaffold is phagocytosed by macrophages, gradually enters lysosomes, and activates antioxidant pathways, including negative regulator of reactive oxygen species (NRROS), sestrin 2 (SESN2), and glucose-6-phosphate dehydrogenase (G6PD), thereby reducing intracellular levels of reactive oxygen and nitrogen species. These processes collectively drive the shift in macrophage polarization from M1 to M2, and accelerate angiogenesis and bone matrix deposition. These findings provide new insights for the design of immunomodulatory bone regenerative materials.
✓ 已验证
产品关联
DOI
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2025
·
IF 3
Qun Gao,Qi Wang,Yanjiao Hu,Fangjie Xin,Jie Chen,Sha Yu,Xinli Chen,Qingqing Lv,Baoxia Cui,Jun Jiao,Xinlin Jiao
Purpose To investigate the impact of SIRT7 on the development of cervical cancer and its relationship with cuproptosis in cervical cancer. Method HeLa and SiHa cells were transfected with lentiviruses for SIRT7 overexpression and knockdown. The effects of SIRT7 on cervical cancer cell proliferation, apoptosis, invasion, and migration were analyzed using CCK8, plate cloning, flow cytometry, Transwell assays, and scratch assays. To verify the relationship between SIRT7 and cuproptosis, we utilized cuproptosis inhibitors and activators. Immunofluorescence, transmission electron microscope, flow cytometry, ELISA, and Western blot were used to analyze copper ion content, mitochondrial ultrastructure, cellular reactive oxygen species, mitochondrial membrane potential, pyruvate levels, cell viability and the cuproptosis-related proteins. Results SIRT7 enhanced the proliferation, migration, and invasion of HeLa and SiHa cells, inhibited apoptosis, and promoted cervical cancer growth. Knocking down SIRT7 caused cuproptosis of HeLa and SiHa cells, characterized by increased Cu 2+ content, disrupted mitochondrial structure, decreased membrane potential, elevated ROS production, and upregulation of cuproptosis-related proteins SLC31A1 and HSP70, and downregulation of FDX1, LIAS and DLAT. Low SIRT7 expression's effect on cuproptosis was reduced by TTM. SIRT7 overexpression inhibited cuproptosis, unlike SIRT7 knockdown. SIRT7 overexpression's inhibitory effect on cuproptosis is altered by rhSLC31A1. Conclusion SIRT7 was recognized as an oncogene in cervical cancer, which boosted cervical cancer cell proliferation and invasion, lowered intracellular copper levels, and prevented cuproptosis. SIRT7 downregulation triggered cuproptosis, inhibiting tumor cell growth.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xiaojiao Di,Zelong Chen,Wenhe Feng,Jiajia Sun,Shuang Chao,Zhichao Pei,Yuxin Pei
Given the copper dependence of malignancies, copper depletion therapy has emerged as a promising anticancer strategy. However, its efficacy is hampered by the metabolic adaptability of tumors, which allows tumor cells switch their metabolic phenotype towards glycolysis to compensate for energy loss. Herein, we developed a glucose oxidase (GOx)-integrated nanoagent (designated as GDP NPs) using a supramolecular copper chelator (denoted as DP NPs) as the backbone. This nanoagent enables the simultaneous intracellular depletion of copper and deprivation of glycolytic substrates. The supramolecular chelator itself is formed via the self-assembly of 2,2′-dipicolylamine-perfunctionalized pillararene; it depletes copper to amplify oxidative stress and reduce adenosine triphosphate production. Concurrently, GOx disrupts the compensatory glycolytic pathway induced by copper depletion through the catalytic breakdown of glucose. Compared with copper depletion monotherapy, the combined effect of copper depletion and metabolic compensation blockade leads to an energy crisis in tumor cells. In vivo experimental results demonstrate that GDP NPs exhibit superior anticancer efficacy relative to DP NPs. Our work presents a novel supramolecular chemistry-based strategy for the concurrent depletion of copper and interference with metabolism, which overcomes the limitation of copper depletion monotherapy due to tumor metabolic adaptability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yuyang Qin,Hongjing Li,Jun Ma
The effect of chloride ion (Cl − ) on the degradation of organic contaminants (OCs) by thermally activated peroxymonosulfate (TAP) was assessed in this study. P -aminobenzoic acid (PABA), sulfadiazine (SDZ), bisphenol A (BPA), and chloramphenicol (CAP) were employed as model OCs with different structure. The degradation efficiencies of PABA, SDZ, BPA, and CAP were respectively 76.4 %, 90.8 %, 10.1 %, and 14.5 % by TAP (70 °C) after 60 min treatment, further raised to 97.6 %, 97.4 %, 55.2 %, and 30.5 % in the presence of Cl − (1 mM). When Cl − concentration increased to 10 mM, PABA and SDZ were completely degraded respectively after 7 min and 10 min, 97.1 % of BPA and 59.5 % of CAP were removed within 60 min. In TAP system, PABA and SDZ were primarily degraded by direct peroxymonosulfate (PMS) oxidation , while BPA and CAP were mostly removed by hydroxyl radical and sulfate radical oxidation. In Cl − /TAP mixtures, the degradation of PABA and BPA was mainly attributed to hypochlorous acid oxidation, SDZ was chiefly removed via direct PMS oxidation, and CAP was principally oxidized by radicals. Furthermore, the intermediate products of OCs degradation during Cl − /TAP oxidation were identified, and primary degradation pathways were proposed. The removal efficiencies of total organic carbon were markedly improved from 19.3 % to 25.3 % by TAP to 52.6 %–69.0 % in Cl/TAP system. The concentrations of typical chlorinated by-products after OCs oxidation were 17.2 μg/L–86.3 μg/L in Cl − /TAP system. Considering the complexity of actual wastewater, 51.3 %–100 % removal efficiencies of OCs by TAP oxidation were achieved in Cl − -containing secondary effluent. Understanding the role of Cl − in OCs degradation is essential for the practical application of Cl − /TAP oxidation in advanced wastewater treatment.
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Tiaoyan Jiang,Tianying Jia,Yipengchen Yin,Tianyu Li,Xinran Song,Wei Feng,Sheng Wang,Li Ding,Yu Chen,Qin Zhang
Radiotherapy is crucial in local cancer management and needs advancements. Tumor cells elevate intracellular copper levels to promote growth and resist radiation; thus, targeted copper delivery to mitochondria could enhance radiotherapy by inducing cuproptosis in tumor cells. In this study, we engineered a multifunctional nanoliposome complex, termed Lipo-Ele@CuO2, which encapsulates both copper peroxide (CuO2) and the copper chelator elesclomol, which can delivery Cu ions to the mitochondria. The Lipo-Ele@CuO2 complex induces mitochondria-mediated cuproptosis in tumor cells and synergistically enhances the efficacy of radiotherapy. CuO2 acts as a copper donor and exhibits inherent sensitivity to acidic environments. Additionally, it depletes intracellular glutathione, thereby sensitizing cells to cuproptosis. Leveraging its pH-responsive properties in the acidic tumor microenvironment, the Lipo-Ele@CuO2 facilitate the controlled release of elesclomol, efficiently delivering copper ions to mitochondria at tumor sites. The combined in vitro and in vivo studies demonstrate that Lipo-Ele@CuO2-based therapy significantly improves antitumor efficacy and exhibits excellent safety profiles, effectively inducing cuproptosis in tumor cells and boosting the effectiveness of radiotherapy. Furthermore, metabolomic and transcriptomic analyses reveal that this combination therapy precipitates significant alterations in tumor energy metabolism, notably repressing genes related to iron–sulfur cluster assembly and glycolysis, thereby confirming the induction of cuproptosis. This therapeutic strategy provides a viable approach for addressing clinical radiotherapy resistance and demonstrates significant translational potential.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Lihua Wang,Yang Deng,Xionghongcan Zhou,Kaiyue Song,Cong Jiang,Feng Zhao,Xianglong Li
Cuproptosis holds promise for breast cancer therapy, yet systemic copper delivery risks off-target toxicity, and intrinsic tumor resistance mechanisms limit its therapeutic efficacy. To overcome this obstacle, a biodegradable hybrid nanomedicine (designated CaO 2 @CuPDA) was designed to dual-amplify cuproptosis through Ca 2+ -overload and photothermal effects for potent immunotherapy. The nanomedicine comprises a calcium peroxide (CaO 2 ) core encapsulated within a copper–polydopamine shell, which stabilizes CaO 2 against premature hydrolysis and confers tumor microenvironment-responsive behavior, photothermal capability, and controlled Cu 2+ release. Upon exposure to the acidic, glutathione (GSH)-rich tumor microenvironment, CaO 2 @CuPDA disintegrates, concurrently releaseing Cu 2+ /Ca 2+ and generating H 2 O 2 . The Fenton-like reaction catalyzed by GSH-reduced Cu + converts H 2 O 2 into hydroxyl radicals, elevating intracellular oxidative stress. Simultaneously, FDX1-mediated reduction of excess Cu 2+ to Cu + inhibits the synthesis of iron‑sulfur cluster proteins to trigger cuproptosis. Critically , this cuproptosis degree is dramatically potentiated by Ca 2+ -overload, increased ROS, and photothermal effects. The amplified cuproptosis elicits robust releasing damage-associated molecular patterns (DAMPs) that activate dendritic cells and recruit cytotoxic T lymphocytes, leading to potent antitumor immunity and suppressed tumor growth in vivo. This work presents a safe and efficient nanoplatform for cuproptosis potentiation, providing a novel strategy for breast cancer treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Rongxiang Zhang,Xuelei Yin,Zenghui Xu,Qikang Yan
Purpose Development of Ce/Cu-modified black ceramics and investigation of the mechanism of anti-osteosarcoma activity. Methods Black ceramics doped with 0.3–0.7 wt% CeO 2 and CuO were prepared and analyzed using energy-dispersive X-ray spectroscopy (EDS), compression stress-strain tests, weight change, and antibacterial assays. MNNG/HOS and MG63 cells were seeded on ceramic substrates, and their adhesion and morphology were analyzed using scanning electron microscopy (SEM) and phalloidin staining. In vitro, we first assessed the anti-tumor effects of ceramic materials, then validated their impact on cuproptosis by measuring Cu 2+ levels and the expression of related proteins, including SLC31A1, HSP70, and ATP7A. Mitochondrial dysfunction and TCA abnormalities were assessed by measuring mitochondrial membrane potential, reactive oxygen species, mitochondrial structure, TCA cycle metabolites (citric acid and lactic acid), ATP levels, and DLAT and LIAS protein levels. The cuproptosis inhibitor TTM, PI3K activator 740Y P, and AKT activator SC79 were used to explore the relationship between cuproptosis and the PI3K/AKT pathway in osteosarcoma cells treated with Ce/Cu-doped black ceramics. Results Our research confirmed the superior anti-tumor properties of the Ce/Cu-modified black ceramic and its potential mechanisms against osteosarcoma. The Ce/Cu-doped black ceramic caused mitochondrial dysfunction and disrupted TCA cycle metabolism, resulting in cuproptosis in MNNG/HOS and MG63 cells. The PI3K/AKT pathway was involved in cuproptosis in osteosarcoma cells treated with Ce/Cu-modified ceramics. Conclusion Our research confirmed that Ce/Cu-modified black ceramics have superior anti-tumor effects and potential mechanisms, confirming the clinical potential of Ce/Cu-modified black ceramics in cancer treatment and providing theoretical basis for the clinical translation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Yi Tang,Lingli Wang,Yu Fu,Zhaohui Wang
It is generally assumed that the degradation efficiency of thermally activated peroxymonosulfate (TAP) oxidation processes is constrained at lower temperatures. This study explores the degradation of organic pollutants using peroxymonosulfate at −40 °C to 80 °C. The oxidative destruction of organic pollutants appeared to follow pseudo first-order kinetics, and an N-shaped degradation profile was observed for Acid Yellow 17 (AY17) as well as for other pollutants. The total organic carbon of AY17 was reduced after 4 h by 23.9 % at −20 °C and by 18.1 % at 80 °C. The direct oxidation of organic pollutants by peroxymonosulfate was significantly enhanced at −20 °C, attributed to the freezing concentration effect. At T ≥ 5 °C, an increase in temperature promoted the oxidation of organic substrates. The involvement of free radicals ( OH and/or SO 4 − ) was verified at T ≥ 60 °C. The degradation rates of pollutants were markedly accelerated in the presence of chloride ions across the tested temperature range, as the freezing concentrated peroxymonosulfate (FCP) and TAP processes may facilitate the generation of hypochlorous acid (HClO). These findings contribute to a deeper understanding of the significant role that temperature plays in modulating the oxidation pathways of persulfate-based processes.
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Junli Yang,Xudong Xie,Qiaojian Duan,Yu Sun,Lin Yang,Bobin Mi,Peng Zhao,Kaiyong Cai
Bone fractures are often accompanied by poor bone healing and a disturbed osteoimmune microenvironment. Inspired by the native osteoimmune microenvironment, a bone-targeting multifunctional nanoplatform (RH@Ag2S@MMSNs) was developed using Ag2S/Mg2+-incorporated rhodamine B hydrazide (RH)-loaded mesoporous silica nanoparticles, which were then conjugated with alendronate and β-cyclodextrin. The nanoplatform not only showed bone-targeting ability ex vivo and in vivo but also exhibited pH-responsive biodegradable capability. The release of RH can efficiently scavenge inflammatory NO, regulate macrophage polarization, and balance the immune microenvironment. Moreover, the combination of Mg2+ and NIR-II imaging-guided mild photothermal therapy (PTT), triggered by near-infrared irradiation, promoted mesenchymal stem cell osteogenic differentiation. In vivo experiments demonstrated that the RH@Ag2S@MMSNs nanoplatform integrated with anti-inflammation, mild PTT, NIR-II imaging, and osteogenic activities significantly accelerated the reconstruction of bone defects. Therefore, this work presents a distinctive strategy to design a bioactive multifunctional nanoplatform for promoting bone tissue regeneration.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Zhiting Yang,Jiahua Tang,Yan Zhang,Xianzhi Hu,Futing Zi,Yue Lin,Jin Wu
Acid mine drainage (AMD) is a persistent and challenging environmental pollution problem. The consequences of AMD include soil pollution, biodiversity loss, and ecosystem collapse. Our study found that rhodamine B acylhydrazone (RBA) exhibits high sensitivity to Fe3+ and can be used as a corrosion inhibitor. This finding led us to hypothesize that the application of RBA is an effective strategy for constructing self-warning and self-healing integrated pH-responsive mercaptopropyl trimethoxysilane (PeopS-SH) coatings. We herein successfully prepared RBA-loaded mesoporous silica nanoparticles (MSN@RBA) designed to exhibit localized, acid-responsive behavior to avoid oxidation site diffusion and enhance wound healing. These MSN@RBA were sensitive to acidic environments, triggering the rapid release of RBA. The experiments showed that MSN@RBA effectively inhibited the oxidation rate of pyrite and enhanced the long-term passivation effect of the coating. Our findings further demonstrated that MSN@RBA reduced the corrosion current density (0.17 μA cm−2), increased the corrosion potential (335 mV), and improved the thickness and density of the coating. The results of release experiments, electrochemical tests and chemical leaching indicate that MSN@RBA releases RBA in acidic environments, and its spironolactam structure opens and forms a complex with Fe3+. The damaged site is fluorescently labeled and repaired. In conclusion, this acid-responsive nanoparticle system presents a new strategy for the treatment of tailings in harsh environments for a long time.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Hongrui Zhang,Ya-nan Zhang,Like Li,Xuegang Li,Yong Zhao
A method for the determination of cholesterol using rhodamine derivatives as chromogenic substrates was proposed in this study. The oxidation of rhodamine B hydrazide (RBH) by hydrogen peroxide (H2O2) was catalyzed by a peroxide-mimicking enzyme to produce the fluorescent product. The reaction was further combined with the oxidase system to achieve indirect determination of cholesterol concentration, which can be catalyzed by cholesterol oxidase to produce H2O2 as an intermediate product. Optical fiber optofluidic lasers (FOFLs), as a sensitive biochemical detection platform, have the ability to distinguish small differences in the amount of fluorescence products generated by the reaction and were used to achieve sensitive cholesterol detection. Under optimized experimental conditions, the dynamic range of three orders of magnitude was obtained in the determination of H2O2 with a lower limit of detection (LOD) of 1.63 μM, and the assay time was as short as 3.5 min. On this basis, the designed cholesterol sensor obtained a linear range of 2.55–652.67 μM with a lower LOD of 2.55 μM and proved to have good selectivity. The possibility of cholesterol determination in human serum was initially tested. The sensor was also characterized by easy signal collection, wash-free procedure, controlled sample consumption, fast assay, low cost, and simple operation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Xiaoyang Li,Han Zhang,Jiahang Liu,Yingying Chu,Mengying Qian,Zhichao Yang,Ming Hua,Weiming Zhang
Chloride ion (Cl − ) is ubiquitous in diverse water bodies, yet poses a longstanding challenge in water pollution control by hindering the efficiency of pollutant degradation. Herein, we proposed a novel concept involving the direct utilization of endogenous Cl − ions in water for rapid water purification within a non-redox zirconium oxide (ZrO 2 )-activated peroxymonosulfate (PMS) system. In this process, PMS was complexed on the ZrO 2 surface through inner-sphere coordination, and effectively activated by the partial electron cloud deviation from Zr(IV) sites to PMS, thereby forming a metastable surface complex with an elevated redox potential. Afterwards, the coexistence of Cl − could trigger the transformation of the reactive complex into free chlorine species, thus leading to a 255.0-fold enhancement in the elimination rate of micropollutants compared with the ZrO 2 /PMS system. Quantitative structure-activity relationship analysis revealed that the ZrO 2 /PMS/Cl − system displayed strong target-dependence towards electron-rich compounds, showcasing a faster oxidation rate for pollutants with higher E HOMO energy levels. Significantly, the novel system performed robust resistance to complex water matrices, achieved low oxidant consumption for pollutant removal, and demonstrated adaptation across a broad range of Cl − concentrations (1.0–100.0 mM). Overall, our findings provide new mechanistic insights into the influence of Cl − ions on PMS activation, which refresh the understanding of the role of Cl − ions on pollutant degradation, and help to guide the treatment design for chloride-containing wastewater.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）