ENZYME AND MICROBIAL TECHNOLOGY
·
2023
·
IF 3.4
Yinghui Zhao,Yongxin Su,Zhenlin Li,Chen Luo,Yijun Chen,Xuri Wu
To solve the insufficient availability of mogrol, an 11α-hydroxy aglycone of mogrosides in Siraitia grosvenorii , snailase was employed as the enzyme to completely deglycosylate LHG extract containing 50% mogroside V. Other commonly used glycosidases performed less efficiently. Response surface methodology was conducted to optimize the productivity of mogrol, which peaked at 74.7% in an aqueous reaction. In view of the differences in water-solubility between mogrol and LHG extract, we employed an aqueous-organic system for the snailase-catalyzed reaction. Of five tested organic solvents , toluene performed best and was relatively well tolerated by snailase. After optimization, biphasic medium containing 30% toluene (v/v) could produce a high-quality mogrol (98.1% purity) at a 0.5 L scale with a production rate of 93.2% within 20 h. This toluene-aqueous biphasic system would not only provide sufficient mogrol to construct future synthetic biology systems for the preparation of mogrosides, but also facilitate the development of mogrol-based medicines.
研究领域:
生物化学
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生物技术
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药学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Zhaojin Zhang,Subhan Mahmood,Ahmad Ali,Zhiyi Ye,Shun Yao
For the first time, the valorization of waste shells from the Siraitia grosvenorii industry involves their conversion into carbon dots (CDs) for the detection of deep eutectic solvents (DESs) concentrations through their fluorescence effect. The material composition of the fruit shells was first analyzed, revealing that lignin constitutes 29.8 % of their total mass. Then four types of DESs were prepared for the extraction of lignin from these shells; among them, choline chloride-lactic acid (ChCl-Lac) achieving the highest extraction rate of 92.49 %. The obtained lignin was characterized and subsequently used as a raw material to synthesize carbon dots possessing tunable fluorescent properties with a quantum yield of 5.73 %. Moreover, it was found that Fe 3 + could quench the fluorescence of the CDs, while the addition of certain DESs could restore the quenched fluorescence. The presence of DESs alone could also induce changes in fluorescence when interacting with the CDs. Based on this effect, a new system for the effective quantitation of the concentration of DESs in aqueous solutions using CDs was established, and DESs were used in several main steps and played multiple roles. This study provides not only a solution for the value-added utilization of solid waste from current fruit industry, but also a new approach for the concentration detection of DESs in aqueous solutions.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
Applied Sciences-Basel
·
2025
·Vol. 15
·期 3
·1020
·
IF 2.5
Yuqiang Liu,Weiqian Yu,Mengfei Bi,Yuting Zhang,Yuan Guan,Tiemin Jiang
The primary objective of this study was to compare the nutrition and metabolite profiles ofSiraitia grosvenoriifrom different regions (namely Yongfu and Longsheng) and processing stages. Our findings showed that freshSiraitia grosvenoriifrom Longsheng contained higher levels of total sugars, protein, and crude fat compared with those from Yongfu, though both regions had similar dietary fiber and ash content. Dried YongfuSiraitia grosvenoriishowed increased nutrient levels. A mineral analysis revealed that freshSiraitia grosvenoriifrom Yongfu had the highest levels of calcium, magnesium, and potassium along with distinct differences in other mineral concentrations compared with Longsheng. Notably, fresh Yongfu fruits had higher mineral content than dried ones, except for aluminum and selenium. Through an untargeted metabolomics analysis, we identified 470 metabolites, showing significant variation between fresh samples from Yongfu and Longsheng and between fresh and dried Yongfu samples. Key metabolites included carboxylic acids, fatty acyls, and organooxygen compounds. Additionally, we observed significant enrichment in metabolic pathways such as phenylpropanoid biosynthesis, galactose metabolism, and linoleic acid metabolism, with notable differences in metabolite regulation depending on the region and processing stage. These findings highlight the influence of regional environmental factors and drying processes on the nutrient and metabolite composition ofSiraitia grosvenorii.
研究领域:
植物学
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食品科学
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代谢组学
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产品关联
DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-9
·
IF 4.38
Huang Xiao-Ping,Lei Lei,Lei Shun-Xin,Zhu Wei-Wei,Yan Jun
Siraitia grosvenorii (LHG) is widely used as a medicinal and edible material around the world. The objective of this study was to develop an effective method for the authentication of the geographical origin of LHG in its main producing area Guangxi, China, which is identified as Chinese Protected Designation of Origin product, against other producing regions in China. The content of 14 elements (K, Na, Ca, P, Mg, Al, B, Ba, Cu, Fe, Mn, Ni, Zn, and Sr) of 114 LHG samples was determined by inductively coupled plasma optical emission spectrometry. Multivariate analysis was then performed to classify the geographical origin of LHG samples. The contents of multielement display an obvious trend of clustering according to the geographical origin of LHG samples based on radar plot and principal component analysis. Finally, three supervised statistical techniques, including linear discriminant analysis (LDA), k -nearest neighbours (k-NN), and support vector machine (SVM), were applied to develop classification models. Finally, 40 unknown LHG samples were used to evaluate the predictive ability of model and discrimination rate of 100%, 97.5% and 100% were obtained for LDA, k -NN, and SVM, respectively. This study indicated that it is feasible to attribute unknown LHG samples to its geographical origin based on its multielement content coupled with chemometric techniques.
研究领域:
植物学
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食品科学
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分析化学
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Huahong Liu,Zhenni Lan,Yun Zhang,Ziwei Zhao,Yuxin Wu,Xuehui Tang,Jinfang Nie
Luohan Guo ( Siraitia grosvenorii ) is a traditional food homologous fruit in China, which is famous owing to its rich natural antioxidant secondary metabolites. However, the impacts of ripening stages and edible parts on high-resolution metabolic profiles of Luohan Guo are poorly understood till now, greatly limiting its comprehensive utilization. In this study, an LC-QTOF/MS-based non-targeted metabolomics approach was carried out to reveal the dynamic accumulation of metabolites in the peel and pulp of Luohan Guo across seven growth stages, and further revelation their potential pharmacological activities and mechanisms in the treatment of oxidative stress using the network pharmacology strategy. The results demonstrated that the majority of bioactive ingredients showed great accumulation at the immature stages in both peels and pulps, and the levels of flavonoid glycosides and terpenoid saponins gradually declined as ripening progressed. Notably, the comparative analysis between the full-blown peel and pulp indicated that the peel had higher concentrations of flavonoids, phenols, and alkaloids, whereas terpenoids and amino acids were the significantly enriched bioactive ingredients in the pulp. In addition, network pharmacology analysis screened 28 key pharmacological compounds in Luohan Guo, which primarily acted on 65 core targets to alleviate diseases induced by oxidative damage and predominantly accumulated in the peels collected between the 10th and 20th days, endowing immature peel with the best antioxidant properties. These findings offer valuabley theoretical insights for understanding the secondary metabolite accumulation in different ripening stages and edible parts of Luohan Guo and improving its comprehensive utilization rate.
研究领域:
植物学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2020
·Vol. 14
·期 4
·2300-2309
·
IF 1.648
Yan Jun,Huang Xiao-Ping,Zhu Wei-Wei
This study was conducted to evaluate the feasibility of using near-infrared spectroscopy (NIRS) for simultaneous determination of total phenolic content (TPC) and antioxidant properties of Siraitia grosvenorii , specifically 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Partial least squares (PLS) coupled with wavelength selection was applied to develop NIRS calibration models based on a set of 80 samples. All models established were cross- and test set validated. The root mean square error of cross validation (RMSECV) and the coefficient of determination for cross validation \(\left( {{\text{R}}_{{{\text{cv}}}}^{2} } \right) \) were calculated to evaluate the performance of models. The results showed good precision of models for TPC ( \({\text{R}}_{{{\text{cv}}}}^{2}\) = 0.9829, RMSECV = 0.0426), DPPH ( \({\text{R}}_{{{\text{cv}}}}^{2}\) = 0.9477, RMSECV = 0.0279), ABTS ( \({\text{R}}_{{{\text{cv}}}}^{2}\) = 0.8958, RMSECV = 0.0154), and FRAP ( \({\text{R}}_{{{\text{cv}}}}^{2}\) = 0.9137, RMSECV = 0.0176). In addition, 20 unknown samples were used to evaluate the predictive ability of the obtained models, and the ratio performance deviation (RPD) for TPC, ABTS, DPPH, and FRAP ranged from 2.70 to 4.57, which indicate that these models had acceptable predictive ability. These results demonstrated that NIRS has the potential to be a time saving and cost-effective method for the determination of both TPC and antioxidant capacities of Siraitia grosvenorii .
研究领域:
食品科学
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分析化学
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植物学
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产品关联
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Ruiming Xiao,Wanci Liao,Guangjuan Luo,Zhenning Qin,Shuangyan Han,Ying Lin
Mogroside V (MV), a sweetener, is one of the major components inSiraitia grosvenorii. In our research, after in vitro incubation with MV for 24 h, the human gut microbiota diversity changed, with an enrichment of the genera Bacteroides, Lactobacillus, Prevotella, Megasphaera, and Olsenella and the inhibition of Clostridium XlVa, Dorea, and Desulfovibrio. Moreover, the synthesis of short-chain fatty acids, such as acetate, propionate, and butyrate, was increased by gut microbiota. According to ultraperformance liquid chromatography–mass spectrometry (UPLC-MS) analysis, MV was decomposed into secondary mogrosides, such as mogroside II/I and mogrol, by gut microbiota. Enhanced antioxidant abilities of the metabolites were found in the broth. The results suggested that MV, as a potential prebiotic, could benefit human health through its interaction with gut microbiota.
研究领域:
营养学
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2023
·
IF 5
Wei Zhou,Siyu Guo,Sheng Zhang,Zhaodi Lu,Ziyi Sun,Yulin Ma,Jinxiu Shi,Hao Zhang
Siraitia grosvenorii seeds are rich in abundant active compounds beneficial to human health. To clarify the digestion characteristics of Siraitia grosvenorii seed flour (SSF) and promote the use of SSF in the processing of functional staple foods, SSF was prepared, its composition and physicochemical properties were studied, and the processing characteristics of SSF-wheat flour were systematically investigated. The results showed that the torque curve and other parameters of the dough were significantly affected by the amount of SSF added. With the increase of SSF proportion, the water absorption showed an increasing trend, while the degree of protein weakening first weakened and then enhanced. At 20% SSF, the dough was more resistant to kneading. In response to an increase in SSF, the L* value decreased significantly, and the a* and b* values increased gradually, while the specific volume decreased gradually. Additionally, the hardness, adhesiveness, and chewiness of the bread enhanced gradually, while its elasticity, cohesiveness, and resilience decreased gradually. After the addition of 30% SSF, the inner tissue of steamed bread was more delicate. With an increase in SSF proportion, the predicted glycemic index (pGI) of steamed bread weakened markedly. Overall, these results showed that SSF, as a kind of food ingredient with hypoglycemic activity, can be used in the production of new functional steamed bread products. This study provides basic research data for the development of products containing S. grosvenorii seed.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Zongjin Pu,Yuhang Ma,Xiaonan Chen,Yixin Liu,Zhen Fang,Ying Peng,Xiaobo Li
Siraitiae Fructus (SF), the dried fruit of Siraitia grosuenorii (Swingle) C. Jeffreyex A. M. Lu et Z. Y. Zhan g , a traditional medicinal and edible herb, is known for its properties in dissipating heat, moistening the lungs and intestines, and promoting defecation, which contains saponins, flavonoids, and polysaccharides. However, the effective substances of SF have not been fully identified. This study systematically evaluated therapeutic efficacy of aqueous extract of Siraitiae Fructus (SFE) and its three fractions: Siraitiae Fructus total saponins (SFTS), polysaccharides (SFP), low-polarity saponins (SFLS). In lipopolysaccharide (LPS)-induced acute lung injury (ALI) rats, both SFE and SFTS demonstrated significant protective effects against pulmonary and colonic tissue damage. SFTS is more advantageous in regulating IL-1β and IL-17A in serum, W/D and TNF-α in lung tissues, and IL-1β, IL-17A in colon tissues, compared with SFLS and SFP. Subsequent validation in influenza A virus (IAV)-infected mice confirmed SFTS's ability to improve survival rates while alleviating viral pneumonia and enterocolitis. The quantitative analysis revealed that Mogroside V (399.728 mg/g) and 11-O-Mogroside V (202.660 mg/g) constituted 60.23% of SFTS. In summary, Both SFE and SFTS could alleviate the lung and colon injury induced by LPS and IAV by restoring the mucosal barrier, reducing the immune response, and decreasing the expression of inflammatory cytokines in the systemic circulation, thereby exerting "lung-gut co-treatment" effect. This study provides a foundation for the further investigation on the effective substances of SF, and also supports the "Lung-gut axis" theory.
研究领域:
药理学
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免疫学
·
中医药学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yongxin Su,Zhenlin Li,Yinghui Zhao,Yijun Chen,Chen Luo,Xuri Wu
Extracts of Siraitia grosvenorii (Swingle), in Chinese known as Luo Han Guo (LHG), is authorized for use as a natural sweetener . LHG is rich in mogroside V that contains five glucoses, but also contains mogroside IIIE and analogues with fewer than three glucose units that cause an unpleasant aftertaste, limiting the use of the extract. Snailase was applied here to convert mogroside V in LHG extract in favor of siamenoside I formation, the sweetest mogroside with a taste similar to sucrose . For application, snailase was immobilized by adsorption to NKA (a macroporous resin), resulting in 10.9 U per g of adsorbed protein. Reuse of the NKA-adsorbed snailase was demonstrated for four cycles, and a continuous production of improved LHG extract at a 0.5 L scale had a productivity of 68.4 g/(L⋅day). The resulting product containing over 50% siamenoside I displayed an improved taste profile with satisfying safety toward HEK293T cells.
研究领域:
食品科学
·
生物化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Wu Mengying,Huang Xiyang,Li Zhouquan,Gan Xiang,Zheng Yumei,Tang Hui,Xiong Jingwei,Yang Xiaoli,Yang Dongye,Zhang Lizhen,Liang Weiyuan,Li Hongtao
Bio-based fluorescent carbon dots (CDs) have emerged as promising material for advanced sensing applications due to their exceptional sensitivity, stability and biocompatibility. However, it was till constrained by ion selectivity and solution suitability especially in physiological solutions (e.g., PBS). Herein, a novel nitrogen-doped carbon dots (N-CDs) fluorescence quenching sensor derived from Siraitia grosvenorii (named “Luo Han Guo” in China) residue was developed for selective determination of trace Cu(II) ions in different solutions. The N-CDs were synthesized through a one-step hydrothermal process utilizing agricultural waste as carbon precursor and urea as the nitrogen source, demonstrating an eco-friendly synthesis strategy. The developed sensor exhibited remarkable anti-interference capability against acid, base, salt and various metal ions while maintaining excellent photostability and biocompatibility (cell viability > 75% at 1000 ug/mL). With a limit of detection (LOD) of 0.13 µmol/L, the system showed superior sensitivity compared to conventional methods. Furthermore, the sensor demonstrated strong universality across solution types and, more importantly, suitability for trace Cu²⁺ quantification in physiological solutions. Experimental results demonstrated that the fluorescence quenching process resulted from the binding of Cu²⁺ to amino groups on CDs, forming complexes through a non-radiative photoinduced electron transfer (PET) mechanism. This PET effect synergizes with static quenching, underlying the high selectivity and sensitivity of N-CDs for Cu²⁺ detection. The integration of novel phytochemical-based sensing materials with a fluorescence quenching mechanism enables highly selective detection of trace Cu²⁺ for environmental and biomedical applications.
研究领域:
分析化学
·
生物传感
·
环境监测
·
纳米材料
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生物医学工程
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药物分析
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2025
·Vol. 14
·期 9
·1065
·
IF 6.6
Fang He,Shenglan Su,Ruihan Song,Yan Li,Luyan Zou,Zongjun Li,Yu Xiao,Aixiang Hou,Ke Li,Yuanliang Wang
Although mulberry leaf (Morus albaL., ML) andSiraitia grosvenorii(SG) individually demonstrate anti-diabetic properties, their combined efficacy against type 2 diabetes mellitus (T2DM) remains unexplored. This study systematically explored the multi-target mechanisms and synergistic potential of the MLSG combination (MLSG) for T2DM intervention. We evaluated the in vitro inhibitory activities of MLSG, ML, and SG on α-amylase and α-glucosidase, alongside antioxidant capacity assessments through DPPH/ABTS radical scavenging, reducing power, and FRAP assays. Bioactive metabolites were identified using non-targeted metabolomics, while core targets and pathways were predicted using network pharmacology and validated through molecular docking. The results reveal MLSG’s significantly enhanced inhibition of α-amylase (IC50= 14.06 mg/mL) and α-glucosidase (IC50= 0.02 mg/mL) compared to individual extracts, exhibiting 1.3–15.5-fold higher potency with synergistic effects (combination index < 1). MLSG also showed improved antioxidant capacity, outperforming SG in DPPH/ABTS+scavenging and reducing power (p< 0.05), and surpassing ML in ABTS+scavenging, reducing power, and FRAP values (p< 0.05). Metabolomics identified 26 MLSG-derived metabolites with anti-T2DM potential, and network analysis pinpointed 26 active components primarily targeting STAT3, AKT1, PIK3CA, EGFR, and MAPK1 to regulate T2DM pathways. Molecular docking confirmed strong binding affinities between these components and core targets. Collectively, MLSG exerts potent synergistic anti-T2DM effects through dual-enzyme inhibition, elevated antioxidant activity, and multi-target pathway regulation, providing a solid foundation for developing MLSG as functional food ingredients.
研究领域:
药理学
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天然产物化学
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代谢疾病
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分子生物学
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功能食品
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中医药学
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系统生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）