ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
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2021
·Vol. 40
·期 7
·1917-1925
·
IF 3.742
Hongyi Xian,Meilin Tang,Ying Chen,Zhenwei Lian,Yanfeng Li,Xiaowu Peng,Dalin Hu
The present study was conducted to assess the genotoxic potential of water from the Helong Reservoir, which was designated as a strategic drinking water source by the Guangdong Provincial Government of China in October 2016. Four kinds of common indigenous fish samples ( Labeo rohita , Cirrhinus molitorella , red tilapia, and Oreochromis niloticus ) were collected at 6 sampling sites during the period from July to November 2020. Fish from the clean drinking water source of the upper reaches of the Liuxihe Reservoir in Guangzhou were collected as the control. Both the alkaline single cell gel electrophoresis assay and the micronucleus test were used to detect DNA damage and the micronucleus rate in erythrocytes of fish samples, respectively. The results indicated that there was a significant increase in comet tail length, Olive tail moment, and micronucleus rates of all fish samples compared with those of the control ( p < 0.05). The order of sensitivity to DNA damage and micronucleus formation was Labeo rohita > Cirrhinus molitorella > red tilapia > Oreochromis niloticus . The results of the 2 kinds of experiments were in perfect agreement with each other. We conclude that there are obvious genotoxic effects from the water in the Helong Reservoir. As a strategic drinking water source, the safety of the Reservoir water quality should be considered. The local government should put the restoration of the Helong Reservoir water quality on the agenda as soon as possible. Environ Toxicol Chem 2021;40:1917–1925. © 2021 SETAC
研究领域:
环境科学
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毒理学
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水生生物学
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水质管理
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yali Shi,Peiyu Wei,Qiuge Shi,Jun Cao,Kexue Zhu,Zhongyuan Liu,Dayong Zhou,Xuanri Shen,Chuan Li
The quality changes in tilapia belly muscle (BM), dorsal muscle (DM) and tail muscle (TM) were studied and the hypothesis of browning of the fillets was revealed during partial freezing. Compared with DM and TM groups, BM samples had higher thiobarbituric acid reactive substances (TBARS) (0.41 mg malondialdehyde eq/kg at 49 d) and K values (61.81% at 42 d) ( P < 0.05). The microstructure of the BM group deteriorated most obviously during storage. Therefore, the BM group was considered to be the fastest to oxidize and deteriorate. In addition, 54 different micromolecular metabolites were identified from tilapia fillets by UHPLC - Q-TOF-MS analysis, and there were significant differences in the micromolecular metabolites in the three parts of tilapia. Therefore, proteins and lipids were degraded by the action of enzymes and microorganisms to produce some amines and small molecular acids, leading to the deterioration of the quality of tilapia fillets.
研究领域:
食品科学
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生物学
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DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Yun-Yao Tu,Jie Leng,Na Zhang,Qin Yang,Hong-Yu Tang,Cheng-Ke Zhu,Wei-Wei Zeng,Ming-Ji Lv,Song Zhu
Tilapia lake virus (TiLV) has caused serious deadly epidemics and still remains a devastating threat to tilapia aquaculture worldwide, with no practical therapeutic measures. In the study, anti-TiLV activities of 22 kinds of drugs are checked to develop a potential strategy for TiLV control. Safety of the selected drugs to E-11 cells is firstly evaluated to determine their maximum safe concentration (MSC). For preliminary screening in vitro , three 10-fold gradient-diluted drug concentrations (10 −2 , 10 −1 and 10 0 * MSC) are prepared. Baicalin shows the strongest inhibition activity on TiLV-induced cytopathic effects with an inhibition rate close to 80 % at 100 mg/L, and followed by favipiravir and amantadine hydrochloride. Baicalin can completely suppress viral infection at 200 mg/L as verification by Western blot and RT-qPCR analysis, and the 90 % inhibitory concentrations of baicalin on TiLV is 61.33 mg/L. Baicalin shows no obvious anti-TiLV activity by pre-treatment of E-11 cells before TiLV infection, while the infectivity of TiLV is significantly weakened following baicalin treatment, and baicalin has a strong anti-TiLV activity at the post-entry stage of TiLV infection. Besides, baicalin shows no obvious effects on the TiLV attachment to E-11 cells, but has significant inhibition on TiLV multiplication. Therefore, baicalin may have a direct virucidal effect, and/or block the viral genome translation and replication. For anti-TiLV activity evaluation in vivo , baicalin can improve the survival rate of TiLV-infected Nile tilapia ( Oreochromis niloticus ) to 81.8 % following treatment with 10 μg/g baicalin for 3 times, while that was 54.5 % for TiLV-infected tilapia without baicalin treatment. Moreover, the viral titers in liver of TiLV-infected tilapia are generally decreased after baicalin treatment. Results so far indicated that baicalin is a promising therapeutic drug against TiLV infection, providing a potential strategy for TiLV control and promoting the healthy development of tilapia aquaculture.
研究领域:
水产养殖学
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病毒学
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药物开发
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DOI
高影响因子
Animal Nutrition
·
2025
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IF 7.5
Xinyao Zhang,Ke Cheng,Lixue Dong,Dexing Zhu,Qingyong Li,Zhongbao Guo,Yongju Luo,Apeng Liu,Juan Tian,Di Peng,Jianmin Zhang,Yangyang Liu,Hua Wen,Ming Jiang
Fava bean is currently utilized as a specialized feed ingredient to enhance the muscle texture of fish. When incorporated at appropriate levels, fava beans can improve fish muscle crispy texture. However, high dietary levels of fava beans may lead to liver damage in fish, although the specific mechanisms behind this effect remain unclear. In this study, 600 genetic improvement of farmed tilapia (GIFT; Oreochromis niloticus ) of uniform size (initial body weight 552.3 ± 10.12 g) and healthy appearance were randomly divided into four groups, each with three replicates of 50 fishes. The fish diets contained different levels of fava beans (0, 50%, 60%, and 70%), designated as D0, D50, D60, and D70, respectively, over a ten-week feeding experiment. A comprehensive analysis of the impact of fava beans on muscle texture and liver health in GIFT was performed. The results showed that 50%, 60%, and 70% dietary fava beans had no significant effect on the growth performance of GIFT ( P > 0.05), but 70% dietary fava beans increased feed conversion ratio (FCR) ( P = 0.022). Consumption of fava beans at levels of 50%, 60%, and 70% in the diet altered GIFT muscle texture ( P < 0.05), and adversely effected serum biochemistry and liver health ( P < 0.05). Non-targeted metabolome analysis of the liver revealed that dietary fava beans may cause irreversible damage to liver cells by disrupting the DNA methylation process ( P < 0.05). This finding enhances the understanding of the mechanisms behind the negative impact of fava bean consumption on liver function. It is crucial to investigate potential strategies to mitigate these adverse effects while maintaining the desired muscle quality when fava beans are used in aquatic feed.
研究领域:
水产养殖学
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分子生物学
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营养学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2025
·Vol. 41
·期 2
·358-366
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IF 3
Dong Jiaxin,Su Rui,Wu Xiaokang,Dong Deming,Wang Yining,Huang Chenni,Qiu Tao,Gu Yu,Hua Xiuyi,Liang Dapeng
The study of the metabolic toxicity of perfluorooctanoic acid (PFOA) is of great importance in assessing its potential impact on aquatic organisms and the aquatic environment, and provides a fundamental scientific basis for environmental protection and sustainable development. In this study, an electrospray ionization (ESI) source capable of direct analysis of solution samples was combined with a high-resolution Orbitrap Fusion Tribrid mass spectrometer to investigate the metabolic perturbations induced by different concentrations of perfluorooctanoic acid (PFOA) and their correlations in skin mucus and liver tissue of tilapia. Seven common metabolites were found in mucus and liver, and nitrogen metabolism pathways were disturbed in all of them. The up-regulation of glutamate in mucus may reflect an increased demand for amino acids and energy, whereas the down-regulation of glutamate in the liver may lead to dysregulation of nitrogen metabolism, protein synthesis and amino acid metabolism. Enrichment analyses suggest a dysregulation of nitrogen metabolism pathways, which may lead to impaired maintenance of tissue function and dysregulated energy metabolism in tilapia.
研究领域:
环境科学
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毒理学
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代谢组学
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产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
·
IF 3.974
Linbao Zhang,Ying Hu,Wei Sun,Si Chen,Xiaoping Jia,Wengui Cai
Di-(2-ethylhexyl) phthalate (DEHP) is currently the most frequently detected phthalic acid esters (PAEs) compounds and can induce diverse toxicities on aquatic organisms. To understand the molecular responses of fish to DEHP, we performed transcriptomic profiles in liver of tilapia ( Oreochromis niloticus ) which were exposed to environmental concentration of DEHP. A total of 30.10 Mb and 30.16 Mb clean reads were retrieved from the control and DEHP treated libraries, respectively. De-novo assembly of all the clean reads obtained 58,585 unigenes. After comparing the two libraries, 2814 and 1790 genes were identified as significantly increased and depressed, respectively. Gene ontology (GO) classification system and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis demonstrated that DEHP significantly disturbed the expression level of genes associated with immunity, endocrine and reproductive system, lipid metabolism and so on. Quantitative real-time PCR was performed to validate the results of RNA-sequencing (RNA-seq) analysis. The resulting data provide new insights for exploring the molecular basis of tilapia response to DEHP exposure.
研究领域:
环境毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Talanta Open
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2025
·
IF 3.7
Xiao-Chen Huang,Shou-Lian Wei,Su Yao,Yu-Tong Lin,Jin-Kui Ma
This study developed a novel dummy molecularly imprinted polymer (DMIP) via emulsion polymerization for rapid, sensitive, and simultaneous detection of four fluoroquinolone (FQ) antibiotic residues in tilapia. The DMIP, coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), was thoroughly characterized for its structure and adsorption properties. Optimized parameters revealed a rough surface morphology with aggregated spherical structures (400-500 nm). Compared to a non-imprinted polymer (NIP), the DMIP exhibited faster adsorption kinetics and enhanced selectivity towards enrofloxacin and related FQ compounds. The DMIP-UPLC-MS/MS method achieved satisfactory recoveries (77-105%) for the four target FQs in tilapia, with intra-day and inter-day relative standard deviations of 3.6-9.2% and 4.0-11.7%, respectively. Method detection limits (LODs) ranged from 0.02-0.06 µg/kg, while quantification limits (LOQs) ranged from 0.06-0.2 µg/kg. This novel method demonstrates excellent potential for highly sensitive and selective monitoring of trace FQ residues in aquatic food products.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Rui Li,Zuli Sun,Yongqiang Zhao,Laihao Li,Xianqing Yang,Shengjun Chen,Ya Wei,Chunsheng Li,Yueqi Wang
The composition of free amino acids (FAAs) and nucleotides has an important effect on fish taste. In this study, the content of FAAs and nucleotides in raw, steamed, boiled and air-fried tilapia fillets were quantitatively analyzed. Thermal processing significantly influenced the composition of FAAs and nucleotides in tilapia. Moreover, there were substantial differences in the nutritional composition of tilapia processed by steaming, boiling and air frying. Compared with raw fillets, the total FAA (TFAA) increased after steaming, boiling, and air frying. The main umami and sweet amino acids in tilapia fillets were aspartic acid and glycine. The total nucleotide content of fresh tilapia was 299 mg/100 g, which increased to 305 and 366 mg/100 g after steaming and air frying, respectively, and decreased to 273 mg/100 g after boiling. The main taste nucleotide inosine monophosphate (IMP) with a taste activity value greater than 1, contributed greatly to the taste of tilapia. Equivalent umami concentration was used for taste evaluation: air frying > steaming > boiling. Therefore, our findings establish that the water-soluble taste substances of tilapia fillets, and the mutual regulatory effects of different compounds varied with different thermal processing methods. Hence, tilapia can be thermally processed according to different taste requirements.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 12
·5266-5277
·
IF 3.9
Xiaoli Liu,Huiyi Li,Dong He,Pingping Wang,Yuteng Li,Kegang Wu
This study was conducted to investigate the effects of dietary supplementation of 0%, 0.2%, 0.35%, and 0.5% cinnamon essential oil on growth performance, fatty acid, and fillet quality of tilapia ( Oreochromis niloticus ). The results of growth experiments showed that the weight gain rate linearly and quadratically increased with increasing cinnamon essential oil doses ( p < 0.05). The results of fatty acids experiments showed that the addition of essential oil significantly decreased the saturated fatty acids levels from 36.67% to 30.82% and increased the polyunsaturated fatty acids (PUFA) levels from 24.55% to 46.89%; especially, the n − 3 PUFA of 0.5% essential oil treatment showed the highest levels. Moreover, the n − 6 PUFA of 0.2% essential oil treatment were increased from 22.17% to 32.99%. The results of fillet quality experiments showed that the hardness and cohesiveness were linearly and quadratically increased with the increasing essential oil doses on days 4 and 7, respectively. The b * values linearly and quadratically decreased as the doses increased on day 7 ( p < 0.05). The total volatile basic nitrogen levels were quadratically decreased with increasing cinnamon essential oil doses on day 7 ( p < 0.05). In general, it can be concluded that cinnamon essential oil presented positive effects on the growth, nutritive values, and meat quality in tilapia.
研究领域:
水产养殖
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营养学
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食品科学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2020
·
IF 0
Hairong Wu,Liangliang Mu,Xiaoxue Yin,Kailiang Han,Fangfang Yan,Enxu Zhou,Biao Han,Zheng Guo,Jianmin Ye
Microfibril-associated glycoprotein 4 (MFAP4), a pattern recognition-like molecule with a fibrinogen-like domain (FBG), has the ability to combine and agglutinate pathogens, playing an essential role in the first line of innate immune defense. In this study, the sequence of Nile tilapia (Oreochromis niloticus) microfibril-associated glycoprotein 4 (OnMFAP4) open reading frame (ORF) was amplified and identified. The ORF of OnMFAP4 is 720 bp of nucleotides and codes for 239 amino acids. Spatial mRNA encoding analysis indicated that OnMFAP4 was highly produced in liver, intestine and head kidney in healthy tilapia, and with the lowest expression in muscle. After challenges with Streptococcus agalactiae ( S. agalactiae ) and Aeromonas hydrophila ( A. hydrophila ), the expression of OnMFAP4 mRNA was prominently produced in the liver, spleen and head kidney. The up-regulation of OnMFAP4 expression was also presented in head kidney monocytes/macrophages (MO/MΦ) and hepatocytes. Recombinant OnMFAP4 ((r)OnMFAP4) could bind and agglutinate both bacterial pathogens. Moreover, (r)OnMFAP4 could take part in the modulation of inflammation and phagocytosis . In conclusion, this study revealed that OnMFAP4 might take effect in host defense against bacterial infection in Nile tilapia , with agglutination and opsonization capability to bacterial pathogens.
研究领域:
免疫学
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分子生物学
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DOI
PubMed
AQUACULTURE
·
2021
·
IF 4.242
Xiaoxue Yin,Nuo Chen,Liangliang Mu,Hao Bai,Hairong Wu,Weiwei Qi,Yu Huang,Jichang Jian,Anli Wang,Jianmin Ye
Hepcidin (Hepc), as an antimicrobial peptide , plays a vital role in regulating the iron homeostasis and participating in innate immunity. In this study, the open reading frame of Nile tilapia ( Oreochromis niloticus ) hepcidin mRNA ( OnHepc ) was amplified, and its tissue distribution and expression pattern in response to bacterial infection and iron overload were characterized. The results showed that the ORF of OnHepc is 273 bp in length and encodes a peptide with 90 amino acids. Hepcidin has a certain degree of conservation among different species. OnHepc is widely expressed in ten tissues of natural Nile tilapia and is most abundant in the liver. The expression of OnHepc was apparently upregulated in liver, spleen, intestine and gills after challenged by S. agalactiae and A. hydrophila . In addition, the OnHepc expression in MO/Mø and hepatocytes was also upregulated after challenged by the above bacteria. On the other hand, the OnHepc transcripts were upregulated in the liver, spleen, intestine and gills after challenged by 1 μM and 10 μM FeCl 3 solutions, and the same trends were observed in the MO/Mø and hepatocytes after challenged by ferric ions. Moreover, the OnHepc expression was significantly upregulated after stimulated by (r)OnIL-6 protein, suggesting that inflammatory responses regulate the expression of OnHepc . In summary, the present study shows that OnHepc probably gets involved in against bacterial infection and regulating the iron homeostasis in O. niloticus .
研究领域:
鱼类生理学
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免疫学
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分子生物学
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DOI
高影响因子
AQUACULTURE NUTRITION
·
2024
·Vol. 2024
·期 1
·6670740
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IF 3
Dingfei Ma,Qiangwei Li,Yuanyuan Xie,Youqin Kong,Zhili Ding,Jinyun Ye,Chenglong Wu,Yan Liu
Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and β -oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.
研究领域:
水产养殖学
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营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）